SELENOW (NM_003009) Human Untagged Clone

CAT#: SC118238

SEPW1 (untagged)-Human selenoprotein W, 1 (SEPW1) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: SELENOW
• Synonyms: selW; SEPW1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118238 sequence for NM_003009 edited (data generated by NextGen Sequencing)
  ATGGCTCTCGCCGTCCGAGTCGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTT
  CAGCTCAAGAAGAAGTTAGAAGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGA
  ACTCCCCAGGCCACCGGGTTCTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAG
  AAGAAAGGCGATGGCTACGTGGACACAGAAAGCAAGTTTCTGAAGTTGGTGGCCGCCATC
  AAAGCCGCCTTGGCTCAGGGCTAA
  
  Clone variation with respect to NM_003009.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003009 unedited
  GGATTTTGTATACGACTCACTATAGGCGGCCGCGNATTCGGCACGAGGGGTTAGTGTGGC
  CCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGAGCCATGGCTCTCGCCGTCCGAGT
  CGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTTCAGCTCAAGAAGAAGTTAGA
  AGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGAACTCCCCAGGCCACCGGGTT
  CTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAGAAGAAAGGCGATGGCTACGT
  GGACACAGAAAGCAAGTTTCTGAAGTTGGTGGCCGCCATCAAAGCCGCCTTGGCTCAGGG
  CTAATGCGCCCTGAAGGCAGAGTCCAGGGACCTTGACCCAGCCCCTCTCAGCAGACGCTT
  CATGATAGGAAGGACTGAAAAGTCTTGTGGACACCTGGTCTTTCCCTGATGTTCTCGTGG
  CTGCTGTTGGGGGCAGAGATTGACGCCCCCGGTCTTTGCCTCTGAGCGGGAGAGTCTGTG
  TGTATGTGTCTTCCCCGGAATCCACACCACCCCACCCTCCTCCTGTCCCGTGGTTTCATC
  ATATCTCTTTGCATACCCCATGTCTTCCCCAGTTGTCCCCTGGAGTTTGGGGGGACATCC
  CGCCTCAGGCATCCTTCTCAGGGGAAGCCAAGAGAGGGCATCAGATGGGTGGGTTTCTGA
  TTGTGGNCACGTTTGNCACCGTTCACGATTCAATAATATTGGATGAATTTAACCAAAAAA
  AAAAAAAAAACTCGCTCTAGATTGCGGCCGCGTCATTAGCTGTTCTGACAGATCCCGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003009 unedited
  AATTCTGTCCGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTGGTTAAATTCA
  TCCAATATTTATTGAATCGTGAACGCTTGCAAACGTTGCCACAATCAGAAACCCACCCAT
  CCTGATGCCTCTCTTGGCTTCCCCTTTTGAAGGATGCCTGAGGCGGGATGTCCCCCCAAA
  CTCCCTGGGACAACTGGGGAAGACATGGGGTATGCAAAGAGATATGATGAAACCACGGGA
  CAGGAGGAGGGTGGGGTGGTGTGGATTCCGGGGAAGACACATACACACAGACTCTCCCGC
  TCAGAGGCTAAGACCGGGGGCGTCAATCTCTGCCCCCAACAGCAGCCACGAGAACACCAG
  GGAAAGACCAGGTGTCCACAAGACTTTTCAGTCCTTTCTATCATGAAGCGCCTGCTGAGA
  GGGGCTGGGTCAAGGTCCCTGGACTCTGCCTTCAGGGCGCATTAGCCCTGAGCCAAGGCG
  GCTTTGATGGCGGCCACCAACTTCAGAAACTTGCTTTCTGTGTCCACGTAGCCCATCGCC
  TTTTTTCTTAGAGTGAATCAACTTCCCGGCTACCATCACTTCAAAGAACCCGGTGGCCTC
  GGGAGTTCCCTCCCCGCAAATGTCCAGCCGGCCCGGGAACCCCATCTTCTAACTTCTTCT
  TGAGCCGAAGATACTTGGCATTGTACCCTCCAGTGCCACCACTAAACCACTCGGCCGGCG
  AGAGCCCTGGTTTCGGGGGTGCCCCATCCGCTGGAGAAGGGGACGTCCCGGCCCAAACTA
  ACTCCTCGTGCCCAATTCCCGGGCCGCCCTAAAACGAGGTCCTATTCCAAAATTTTGAGG
  GATACCCAAACGACCTCTGTTCATCAGAACCCCCAACGTTACAGCTTCCGGGCAATTTGC
  GCAACGGGCGCGTGTTTTTCCCCCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003009
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003009.2, NP_003000.1
• RefSeq Size: 893 bp
• Locus ID: 6415
• Cytogenetics: 19q13.33
• Gene Summary: 'This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle, heart and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A retroprocessed pseudogene of this locus has been identified on chromosome 1. [provided by RefSeq, Aug 2017]'