SELENOW (NM_003009) Human Untagged Clone

CAT#: SC118238

SEPW1 (untagged)-Human selenoprotein W, 1 (SEPW1) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)


  "NM_003009" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Symbol SELENOW
Synonyms selW; SEPW1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118238 sequence for NM_003009 edited (data generated by NextGen Sequencing)
ATGGCTCTCGCCGTCCGAGTCGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTT
CAGCTCAAGAAGAAGTTAGAAGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGA
ACTCCCCAGGCCACCGGGTTCTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAG
AAGAAAGGCGATGGCTACGTGGACACAGAAAGCAAGTTTCTGAAGTTGGTGGCCGCCATC
AAAGCCGCCTTGGCTCAGGGCTAA

Clone variation with respect to NM_003009.2
>OriGene 5' read for NM_003009 unedited
GGATTTTGTATACGACTCACTATAGGCGGCCGCGNATTCGGCACGAGGGGTTAGTGTGGC
CCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGAGCCATGGCTCTCGCCGTCCGAGT
CGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTTCAGCTCAAGAAGAAGTTAGA
AGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGAACTCCCCAGGCCACCGGGTT
CTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAGAAGAAAGGCGATGGCTACGT
GGACACAGAAAGCAAGTTTCTGAAGTTGGTGGCCGCCATCAAAGCCGCCTTGGCTCAGGG
CTAATGCGCCCTGAAGGCAGAGTCCAGGGACCTTGACCCAGCCCCTCTCAGCAGACGCTT
CATGATAGGAAGGACTGAAAAGTCTTGTGGACACCTGGTCTTTCCCTGATGTTCTCGTGG
CTGCTGTTGGGGGCAGAGATTGACGCCCCCGGTCTTTGCCTCTGAGCGGGAGAGTCTGTG
TGTATGTGTCTTCCCCGGAATCCACACCACCCCACCCTCCTCCTGTCCCGTGGTTTCATC
ATATCTCTTTGCATACCCCATGTCTTCCCCAGTTGTCCCCTGGAGTTTGGGGGGACATCC
CGCCTCAGGCATCCTTCTCAGGGGAAGCCAAGAGAGGGCATCAGATGGGTGGGTTTCTGA
TTGTGGNCACGTTTGNCACCGTTCACGATTCAATAATATTGGATGAATTTAACCAAAAAA
AAAAAAAAAACTCGCTCTAGATTGCGGCCGCGTCATTAGCTGTTCTGACAGATCCCGGTG
>OriGene 3' read for NM_003009 unedited
AATTCTGTCCGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTGGTTAAATTCA
TCCAATATTTATTGAATCGTGAACGCTTGCAAACGTTGCCACAATCAGAAACCCACCCAT
CCTGATGCCTCTCTTGGCTTCCCCTTTTGAAGGATGCCTGAGGCGGGATGTCCCCCCAAA
CTCCCTGGGACAACTGGGGAAGACATGGGGTATGCAAAGAGATATGATGAAACCACGGGA
CAGGAGGAGGGTGGGGTGGTGTGGATTCCGGGGAAGACACATACACACAGACTCTCCCGC
TCAGAGGCTAAGACCGGGGGCGTCAATCTCTGCCCCCAACAGCAGCCACGAGAACACCAG
GGAAAGACCAGGTGTCCACAAGACTTTTCAGTCCTTTCTATCATGAAGCGCCTGCTGAGA
GGGGCTGGGTCAAGGTCCCTGGACTCTGCCTTCAGGGCGCATTAGCCCTGAGCCAAGGCG
GCTTTGATGGCGGCCACCAACTTCAGAAACTTGCTTTCTGTGTCCACGTAGCCCATCGCC
TTTTTTCTTAGAGTGAATCAACTTCCCGGCTACCATCACTTCAAAGAACCCGGTGGCCTC
GGGAGTTCCCTCCCCGCAAATGTCCAGCCGGCCCGGGAACCCCATCTTCTAACTTCTTCT
TGAGCCGAAGATACTTGGCATTGTACCCTCCAGTGCCACCACTAAACCACTCGGCCGGCG
AGAGCCCTGGTTTCGGGGGTGCCCCATCCGCTGGAGAAGGGGACGTCCCGGCCCAAACTA
ACTCCTCGTGCCCAATTCCCGGGCCGCCCTAAAACGAGGTCCTATTCCAAAATTTTGAGG
GATACCCAAACGACCTCTGTTCATCAGAACCCCCAACGTTACAGCTTCCGGGCAATTTGC
GCAACGGGCGCGTGTTTTTCCCCCCCN
Restriction Sites NotI-NotI     
ACCN NM_003009
Insert Size 800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003009.2, NP_003000.1
RefSeq Size 893 bp
Locus ID 6415
Cytogenetics 19q13.33
Gene Summary 'This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle, heart and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A retroprocessed pseudogene of this locus has been identified on chromosome 1. [provided by RefSeq, Aug 2017]'

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