SFRP1 (NM_003012) Human Untagged Clone

CAT#: SC118246

SFRP1 (untagged)-Human secreted frizzled-related protein 1 (SFRP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFRP1
• Synonyms: FRP; FRP-1; FRP1; FrzA; SARP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118246 sequence for NM_003012 edited (data generated by NextGen Sequencing)
  ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG
  CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG
  TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC
  ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC
  CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG
  CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC
  TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC
  GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC
  CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC
  CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA
  CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT
  GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG
  AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG
  CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG
  CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA
  ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTGA
  
  Clone variation with respect to NM_003012.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003012 unedited
  TATAGGCGGCCGCGAATTCGGCACGAGGGCAGCCCCGACGTCGCGGAGACAGGGCGCAGA
  GCCGGCATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCCCTGGGCGTGCTGCTG
  GCGCTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTC
  CAGTCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTG
  GACATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCC
  AACCTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCCAGCAGCTGGGTGCC
  CCTGCTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCC
  CGTCTGCCTGGACCGGCCCATCTACCCGTGTTCGCTGGCTCTGCGAGGCCGTGCGCGACT
  CGTGCGAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACA
  AGTTCCCGAGGGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCC
  AAGCCCCAAGACAAACGGGTGTGTCCTCCCTGTGACACGAGTTGAAATCTGAGGCCATCA
  TTGGACATCTCTGTGCAANNCGAGTTTGCNCTGGAGGATGAAATAAAAAGAAGTGAAAAA
  GAAATGGCGACAGGAAAATGGTCCCCAGAGAAGAAGCCCTGGGGGTTGGGGGCCATCAAN
  AANAAAGACTGAAAAACCTTGGGCTGACTTGAAAAAGGGGGGTGGATTGCCCTGGCACCA
  GTGGACAACTTAGCCCCATTTCTCATATGGCCCAAAGGGGGAAACCAGACTTTGTGGCGG
  CATCCCACATGGGACAGAAAAAAAGGGGTCAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003012 unedited
  NAATCTATGNACGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTGATTAAT
  TGAGTGACTTTAGTCAAGTGAATATTGATACATGNGCTGAAAAAGCATGAAAATAAAATG
  AACACGTACGGGAATTACTATTAACATAAGCGATAACATCAAAACATCTGGTAAAATGCA
  GTTAAAAAAACAACACAAATGAAATGGAATGTAAAACATTTTCACAGTATTCAAAGCTTT
  TGTAAGAGACTTAGGATCTAAAAGAGGAGTACTACAGGAAGAAACTAGAGGAAACGGGAA
  TTTCATCCATGTCCTGTGTATCTGCTGGCAACAGGTCAGAACGGCCAGTATGTTATTCCC
  TGCAGGCTGCCTAGGGTGCTCTCCTCAAACAGATCACCTGAGCCTCCTGCATCTATGAAG
  TTATGACACAGCAACCAGTTACTCAGAGTCTGATGAGAAAAACAGATTTTAGGTTTGGGA
  AATGGGATTACTGTAATTTACACATCCAAATGCAAACTGGAGCTCTGATTGAATTCTACC
  CTGNGGAGAACTTGATGCTAACCCACAGGTACCAAGAGCCAAGTGTTACACAGGATATTN
  TAAAAATAAAATGTTTTTGGAATCCTCACCTNCCATGCTATCTTCTAAGAAACTACAAAT
  ATTCTTCAAAGATTTACCTGAGTTCTGCCAAGGACCTCCCAGGACTCTATCCAGAATGGA
  TATTTGTAAAGCTTTACAAATNCCACCTTGGCCTTAGCGAATATTAGGAAATNACAGGGC
  AACCTCCTCTCTCGGGAACCATGACCAGGCCATTAGTCTGACATTGGGTTTGTTAAAACC
  TTGGGGGAAAAACAAAGCTCGGGATAATGCACTCTTGGCCTCAAGAAAGCCCCTTTTGGG
  TTTGAAATGGCGGAGGGACAAAAAAACTTGTATGGGTTAAAACTCAAATAGAACTATTGA
  CCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003012
• Insert Size: 4300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A True Clone.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003012.3, NP_003003.3
• RefSeq Size: 4465 bp
• RefSeq ORF: 945 bp
• Locus ID: 6422
• Cytogenetics: 8p11.21
• Domains: FRI, NTR
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
• Protein Pathways: Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]'