SFRP1 (NM_003012) Human Untagged Clone

CAT#: SC118246

SFRP1 (untagged)-Human secreted frizzled-related protein 1 (SFRP1)


  "NM_003012" in other vectors (6)

Reconstitution Protocol

USD 660.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SFRP1
Synonyms FRP; FRP-1; FRP1; FrzA; SARP2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118246 sequence for NM_003012 edited (data generated by NextGen Sequencing)
ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG
CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG
TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC
ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC
CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG
CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC
TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC
GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC
CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC
CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA
CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT
GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG
AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG
CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG
CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA
ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTGA

Clone variation with respect to NM_003012.4
>OriGene 5' read for NM_003012 unedited
TATAGGCGGCCGCGAATTCGGCACGAGGGCAGCCCCGACGTCGCGGAGACAGGGCGCAGA
GCCGGCATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCCCTGGGCGTGCTGCTG
GCGCTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTC
CAGTCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTG
GACATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCC
AACCTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCCAGCAGCTGGGTGCC
CCTGCTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCC
CGTCTGCCTGGACCGGCCCATCTACCCGTGTTCGCTGGCTCTGCGAGGCCGTGCGCGACT
CGTGCGAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACA
AGTTCCCGAGGGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCC
AAGCCCCAAGACAAACGGGTGTGTCCTCCCTGTGACACGAGTTGAAATCTGAGGCCATCA
TTGGACATCTCTGTGCAANNCGAGTTTGCNCTGGAGGATGAAATAAAAAGAAGTGAAAAA
GAAATGGCGACAGGAAAATGGTCCCCAGAGAAGAAGCCCTGGGGGTTGGGGGCCATCAAN
AANAAAGACTGAAAAACCTTGGGCTGACTTGAAAAAGGGGGGTGGATTGCCCTGGCACCA
GTGGACAACTTAGCCCCATTTCTCATATGGCCCAAAGGGGGAAACCAGACTTTGTGGCGG
CATCCCACATGGGACAGAAAAAAAGGGGTCAAAA
>OriGene 3' read for NM_003012 unedited
NAATCTATGNACGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTGATTAAT
TGAGTGACTTTAGTCAAGTGAATATTGATACATGNGCTGAAAAAGCATGAAAATAAAATG
AACACGTACGGGAATTACTATTAACATAAGCGATAACATCAAAACATCTGGTAAAATGCA
GTTAAAAAAACAACACAAATGAAATGGAATGTAAAACATTTTCACAGTATTCAAAGCTTT
TGTAAGAGACTTAGGATCTAAAAGAGGAGTACTACAGGAAGAAACTAGAGGAAACGGGAA
TTTCATCCATGTCCTGTGTATCTGCTGGCAACAGGTCAGAACGGCCAGTATGTTATTCCC
TGCAGGCTGCCTAGGGTGCTCTCCTCAAACAGATCACCTGAGCCTCCTGCATCTATGAAG
TTATGACACAGCAACCAGTTACTCAGAGTCTGATGAGAAAAACAGATTTTAGGTTTGGGA
AATGGGATTACTGTAATTTACACATCCAAATGCAAACTGGAGCTCTGATTGAATTCTACC
CTGNGGAGAACTTGATGCTAACCCACAGGTACCAAGAGCCAAGTGTTACACAGGATATTN
TAAAAATAAAATGTTTTTGGAATCCTCACCTNCCATGCTATCTTCTAAGAAACTACAAAT
ATTCTTCAAAGATTTACCTGAGTTCTGCCAAGGACCTCCCAGGACTCTATCCAGAATGGA
TATTTGTAAAGCTTTACAAATNCCACCTTGGCCTTAGCGAATATTAGGAAATNACAGGGC
AACCTCCTCTCTCGGGAACCATGACCAGGCCATTAGTCTGACATTGGGTTTGTTAAAACC
TTGGGGGAAAAACAAAGCTCGGGATAATGCACTCTTGGCCTCAAGAAAGCCCCTTTTGGG
TTTGAAATGGCGGAGGGACAAAAAAACTTGTATGGGTTAAAACTCAAATAGAACTATTGA
CCTA
Restriction Sites NotI-NotI     
ACCN NM_003012
Insert Size 4300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation A True Clone.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003012.3, NP_003003.3
RefSeq Size 4465 bp
RefSeq ORF 945 bp
Locus ID 6422
Cytogenetics 8p11.21
Domains FRI, NTR
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
Protein Pathways Wnt signaling pathway
Gene Summary 'This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]'

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