SIAT4A (ST3GAL1) (NM_003033) Human Untagged Clone

CAT#: SC118260

ST3GAL1 (untagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST3GAL1
• Synonyms: 1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA; ST3GalA.1; ST3GalIA; ST3O
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118260 sequence for NM_003033 edited (data generated by NextGen Sequencing)
  ATGGTGACCCTGCGGAAGAGGACCCTGAAAGTGCTCACCTTCCTCGTGCTCTTCATCTTC
  CTCACCTCCTTCTTCCTGAACTACTCCCACACCATGGTGGCCACCACCTGGTTCCCCAAG
  CAGATGGTCCTGGAGCTCTCCGAGAACCTGAAGAGACTGATCAAGCACAGGCCTTGCACC
  TGCACCCACTGCATCGGGCAGCGCAAGCTCTCGGCCTGGTTCGATGAGAGGTTCAACCAG
  ACCATGCAGCCGCTGCTGACTGCCCAGAACGCGCTCTTGGAGGACGACACCTACCGATGG
  TGGCTGAGGCTCCAGCGGGAGAAGAAGCCCAATAACTTGAATGACACCATCAAGGAGCTG
  TTCAGAGTGGTGCCTGGGAATGTGGACCCTATGCTGGAGAAGAGGTCGGTGGGCTGCCGG
  CGCTGCGCCGTTGTGGGCAACTCGGGCAACCTGAGGGAGTCTTCTTATGGGCCTGAGATA
  GACAGTCACGACTTTGTCCTCAGGATGAACAAGGCGCCCACGGCAGGGTTTGAAGCTGAT
  GTTGGGACCAAGACCACCCACCATCTGGTGTACCCTGAGAGCTTCCGGGAGCTGGGAGAT
  AATGTCAGCATGATCCTGGTGCCCTTCAAGACCATCGACTTGGAGTGGGTGGTGAGCGCC
  ATCACCACGGGCACCATTTCCCACACCTACATCCCGGTTCCTGCAAAGATCAGAGTGAAA
  CAGGATAAGATCCTGATCTACCACCCAGCCTTCATCAAGTATGTCTTTGACAACTGGCTG
  CAAGGGCACGGGCGATACCCATCTACCGGCATCCTCTCGGTCATCTTCTCAATGCATGTC
  TGCGATGAGATGGACTTGTACGGCTTCGGGGCAGACAGCAAAGGGAACTGGCACCACTAC
  TGGGAGAACAACCCATCCGCGGGGGCTTTTCGCAAGACGGGGGTGCACGATGCAGACTTT
  GAGTCTAACGTGACGGCCACCTTGGCCTCCATCAATAAAATCCGGATCTTCAAGGGGAGA
  TGA
  
  Clone variation with respect to NM_003033.3
  261 c=>t;850 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003033 unedited
  NTTACCGTTCCGTATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGA
  GGGAGGAGGAGGAGGAAGAAGAGCGGAGAGAGAAGGAAGAGGCGATGTGAGCTGGGAAGG
  GGGCAAGTGTCCGGGACACCCACACCCCTGTATTCTCCTCCGAACCCCTTCATGCCCAAA
  TCCCGGAAACTCCAGCGTGTCTCCAGCCGTGTTGGTACCATTTTCAGATTTCATCTTCCT
  AAACTGGAAATGTCAATGAGAGGAAATTAACACCCCCAAGAGCTGCAGTGAGCAAATGCA
  TTGAGCTTGGGTCAGGACAATTCCATTTGGGGACCAGAGATGGACGGTCACTCAGCCTAT
  GGAGATGAAGAAACTGAGGTTCAGAGAGGTTAAGAGACTCCACTGAGGTCACACAGCCGA
  TGACAGACAACCTTCTGTGCCTTCATCAAGCTGGTTGTGTACCCACCATGTCCCTGGCGA
  CAGGATGGGAAAGAAAAAGCCCTAATTAAGGATCGTCAGAAACCACAGTTGGAGGAGGAC
  GGCAGAGACAGTTTCCCTCCCCGCTATACCAACACCCTTCCTTCGAGGTCCTCGCTCCTG
  AGGGACCCTGGACTGTCACAGAGATTAATGACCCCTTATCTTCTTTGGATGTGAAAGGAA
  ATCACTGGTTAAAGCTTGATCGAGAGACATTATCAGCTCTTTAAGGATTGCAGGAGAATA
  GGCTACTTTATTTTCTGAAAAGGAGGAGTTCCTGCTACCCATCGTGGGAGGCCACCATCA
  GGACTGCGAAGATGGTGACCCTGCGGAAGAGACCCTGAAGTGCTCACTTCCTCGTGCTCT
  TCATCTTCCTCACTCCTTCTTCCTGACTACTCCACACCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003033 unedited
  GCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTCAAAGACAGGGTCTCACTCTGTTGCC
  CAGGCTGGAGTGCAGTGGTGTGATCACAGCCCACTGCAGCCTCGAACTCCTAGGCACAAG
  AGATCCTCCCTCCTCAGCCTCCCTAGTAGCTGGGGCTACAAGTGTGTGCCACCGTGCCTG
  GCTAATTTTTTTAAATCTTTTATTTTTGTAGAGACAGGAGTCTCACTATGTTGCCCAGGC
  TGGTCTTGAACTCCTGGGCTCAAGTGATCCTCCCGCCTCGGCCTCCCAAAGTGTTCAGAT
  TACAGGTGTGAGCCACGGCACCTGGCCTCAAGTTTCTGAGAAAATCTTTGGGGTAGGAGC
  CTCTGGTGGCTCATAAATGAGCCTTGGGAAAATGACAGGGTTATCACGCCAAGCAAGAGG
  CCAGCCTTCCTGCAAAGAAGGGCAGCATCTCGCCCCGAGCGCCAACACCGCCTGACACGT
  TTCTGTGCTGGAGGTAATCCCCCCTCAAGTCTCTGCCCATCCTTCCGGGAAACCGCACTG
  ACTCTAAAAACATACGGCATCTTACACATTATTATCACCAAGCTGGCCCAATATTCCAAA
  AATGTTTCTCACCGGCGCGAGGCTATACTTTCTCCCCACTTTCACCTTCACTTCGTAAAT
  AACTGGGTGGTCCCCCCCCACCCCCACAACTCCGCATACCTAACTATTACTTCCACCCAA
  CTAGGCACATCCTATCCTATACTTCTTGTCACCCCCTCCTCACATGCCTATCTATCCCTC
  ATTCCATTTTTCTCACTCTTCCGACACCCATAAGCAATCTCTATTTTTCTTTCTATCTCT
  GTTTGCTCTTTCTCCGCCCTCTTTTCCTCCCCCTCCCCCCCCTACTCCCCACCGCACCTA
  TGTCTGTTTCATCTCTTCTCTATACCCATCTGTATTCACTTCTTAGTCATCATCACCCCC
  CCCCCCCCCTCCTCCCCCCCCCCCGCTTCCATCTTTTATTCTTCATTTTCAACCCTCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003033
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003033.2, NP_003024.1
• RefSeq Size: 2911 bp
• RefSeq ORF: 1023 bp
• Locus ID: 6482
• Cytogenetics: 8q24.22
• Domains: Glyco_transf_29
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Keratan sulfate biosynthesis, Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.