ABCE1 (NM_002940) Human Untagged Clone

CAT#: SC118287

ABCE1 (untagged)-Human ATP-binding cassette, sub-family E (OABP), member 1 (ABCE1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCE1
• Synonyms: ABC38; OABP; RLI; RLI1; RNASEL1; RNASELI; RNS4I
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118287 sequence for NM_002940 edited (data generated by NextGen Sequencing)
  ATGGCAGACAAGTTAACGAGAATTGCTATTGTCAACCATGACAAATGTAAACCTAAGAAA
  TGTCGACAGGAATGCAAAAAGAGTTGTCCTGTAGTTCGAATGGGAAAATTATGCATAGAG
  GTTACACCCCAGAGCAAAATAGCATGGATTTCCGAAACTCTTTGTATTGGTTGTGGTATC
  TGTATTAAGAAATGCCCCTTTGGCGCCTTATCAATTGTCAATCTACCAAGCAACTTGGAA
  AAAGAAACCACACATCGATATTGTGCCAATGCCTTCAAACTTCACAGGTTGCCTATCCCT
  CGTCCAGGTGAAGTTTTGGGATTAGTTGGAACTAATGGTATTGGAAAGTCAACTGCTTTA
  AAAATTTTAGCAGGAAAACAAAAGCCAAACCTTGGAAAGTACGATGATCCTCCTGACTGG
  CAGGAGATTTTGACTTATTTCCGTGGATCTGAATTACAAAATTACTTTACAAAGATTCTA
  GAAGATGACCTAAAAGCCATCATCAAACCTCAATATGTAGACCAGATTCCTAAGGCTGCA
  AAGGGGACAGTGGGATCTATTTTGGACCGAAAAGATGAAACAAAGACACAGGCAATTGTA
  TGTCAGCAGCTTGATTTAACCCACCTAAAAGAACGAAATGTTGAAGATCTTTCAGGAGGA
  GAGTTGCAGAGATTTGCTTGTGCTGTCGTTTGCATACAGAAAGCTGATATTTTCATGTTT
  GATGAGCCTTCTAGTTACCTAGATGTCAAGCAGCGTTTAAAGGCTGCTATTACTATACGA
  TCTCTAATAAATCCAGATAGATATATCATTGTGGTGGAACATGATCTAAGTGTATTAGAC
  TATCTCTCCGACTTCATCTGCTGTTTATATGGTGTACCAAGCGCCTATGGAGTTGTCACT
  ATGCCTTTTAGTGTAAGAGAAGGCATAAACATTTTTTTGGATGGCTATGTTCCAACAGAA
  AACTTGAGATTCAGAGATGCATCACTTGTTTTTAAAGTGGCTGAGACAGCAAATGAAGAA
  GAAGTTAAAAAGATGTGTATGTATAAATATCCAGGAATGAAGAAAAAAATGGGAGAATTT
  GAGCTAGCAATTGTAGCTGGAGAGTTTACAGATTCTGAAATTATGGTGATGCTGGGGGAA
  AATGGAACGGGTAAAACGACATTTATCAGAATGCTTGCTGGAAGACTTAAACCTGATGAA
  GGAGGAGAAGTACCAGTTCTAAATGTCAGTTATAAGCCACAGAAAATTAGTCCCAAATCA
  ACTGGAAGTGTTCGCCAGTTACTACATGAAAAGATAAGAGATGCTTATACTCACCCACAA
  TTTGTGACCGATGTAATGAAGCCTCTGCAAATTGAAAACATCATTGATCAAGAGGTGCAG
  ACATTATCTGGTGGTGAACTACAGCGAGTAGCTTTAGCCCTTTGCTTGGGCAAACCTGCT
  GATGTCTATTTAATTGATGAACCATCTGCATATTTGGATTCTGAGCAAAGACTGATGGCA
  GCTCGAGTTGTCAAACGTTTCATACTCCATGCAAAAAAGACAGCCTTTGTTGTGGAACAT
  GACTTCATCATGGCCACCTATCTAGCGGATCGCGTCATCGTTTTTGATGGTGTTCCATCT
  AAGAACACAGTTGCAAACAGTCCTCAAACCCTTTTGGCTGGCATGAATAAATTTTTGTCT
  CAGCTTGAAATTACATTCAGAAGAGATCCAAACAACTATAGGCCACGAATAAACAAACTT
  AATTCAATTAAGGATGTAGAACAAAAGAAGAGTGGAAACTACTTTTTCTTGGATGATTAG
  
  Clone variation with respect to NM_002940.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002940 unedited
  TGTTCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCGGCTGGG
  CACCGCCATTTTGGCCGGTGGCCGTGAGAACACGCTGTGTGGCTGAAAAGTGAAGGCAAG
  AGCTGATTTGGCCTCTGTGCTCCCCTCCGCAAGGGGATCGTTTTCTCCAGAAGAGCTGGA
  TATTCTTTCGCCCAGTTATGGCAGACAAGTTAACGAGAATTGCTATTGTCAACCATGACA
  AATGTAAACCTAAGAAATGTCGACAGGAATGCAAAAAGAGTTGTCCTGTAGTTCGAATGG
  GAAAATTATGCATAGAGGTTACACCCCAGAGCAAAATAGCATGGATTTCCGAAACTCTTT
  GTATTGGTTGTGGTATCTGTATTAAGAAATGCCCCTTTGGCGCCTTATCAATTGTCAATC
  TACCAAGCAACTTGGAAAAAGAAACCACACATCGATATTGTGCCAATGCCTTCAAACTTC
  ACAGGTTGCCTATCCCTCGTCCAGGTGAAGTTTTGGGATTAGTTGGAACTAATGGTATTG
  GAAAGTCAACTGCTTTAAAAATTTTAGCAGGAAAACAAAAGCCAAACCTTGGAAAGTACG
  ATGATCCTCCTGACTGGCAGGAGATTTTGACTTATTTCCGTGGATCTGAATTACAAAATT
  ACTTTACAAAGATTCTAGAAGATGACCTANAAGCCATCATCAAACCTCAATATGTAGACC
  AGATTCCTANAGCTGCAAAGGGGACAGTGGGGATCTATTTGGNACCGAAAGATGAAACAN
  AGACACAGGCATTTGTATGTCAGCAGCTTGATTTAACCCACCCTANAGAAACGAATGTTG
  NNAGATCTTTCACGGAGGAGAGTGCAGAGATTTGCTTGTGCTGNCGTTGGCATACAAAAG
  CTGATATNTTCATGGTNGATGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002940 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAACATAATCCTGATG
  TTTATTTATCAAATGTCAATCAGTGGAATCTTTGACTGGCTTTTAAAATAAGGGCAGTTT
  CATTACACTGGCATACTTGGCAAACCAGGTAAGTACAGAAACCCTTTTCAAGATTTAAAA
  AATGGAGCAACACTGGTCAGCATTTGGTACCGTGAATATAATACACCTACTGAAAATCTT
  CCCATACATCATGAAAACTTGGAAGTAATTTGTATAGCTTGAAATGTCTTCATTTTAGAA
  TATTATGCACAAAAGAGCCTCTTCAGGAACAGAAAAGTGGCATTTTCTGTGTTTCAACTA
  CAATTTAGAACCCAACTGGCTTTTTATGTTATATTAAGTATATTATACTTCAAGTTCCAG
  AGTATGTGGGGCATAAAATCCTGATTCAAGTTTTATATGACAAAAAATTCTAGTAAATAC
  TCCTTTTAATAAATGGCTTATCAATATTCTCAGAGTCAGTCTAATCATCCAAGAAAAAGT
  AGTTTCCACTCTTCTTTTGTTCTACATCCTTAATTGAATTAAGTTTGTTTATTCGTGGCC
  TATAGTTGTTTGGATCTCTTCTGATGTAATTTCAAGCTGAGACAAAAATTATTCATGCCA
  GCCAAAGGGGTTGAGGACTGTTTGCACTGTGTTCTAGATGGAACCCATCAAAACCATGAC
  GCGATCCGGTAGATAGGTGGCATGATGAAATCATGTTCCAACCAAAGCTGTCTTTTTTGA
  TGGAGTATGAAAGTTTGAAACTCGACCGCCTTAATCTTGTTAAAATCAAATTGCGATGGG
  TATCATTAATAAATTACAGGTTGCCAACAAGGCTAAGTATCTTGTGATACCACAAAAAGG
  TTGCCTTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002940
• Insert Size: 2910 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002940.1, NP_002931.1
• RefSeq Size: 3568 bp
• RefSeq ORF: 1800 bp
• Locus ID: 6059
• Cytogenetics: 4q31.21
• Domains: fer4, ABC_tran, AAA, RLI
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.