RPL15 (NM_002948) Human Untagged Clone

CAT#: SC118292

RPL15 (untagged)-Human ribosomal protein L15 (RPL15)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL15
• Synonyms: DBA12; EC45; L15; RPL10; RPLY10; RPYL10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118292 sequence for NM_002948 edited (data generated by NextGen Sequencing)
  ATGGGTGCATACAAGTACATCCAGGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGCGC
  TTTCTTCTGAGGGTCCGCTGCTGGCAGTACCGCCAGCTCTCTGCTCTCCACAGGGCTCCC
  CGCCCCACCCGGCCTGATAAAGCGCGCCGACTGGGCTACAAGGCCAAGCAAGGTTACGTT
  ATATATAGGATTCGTGTTCGCCGTGGTGGCCGAAAACGCCCAGTTCCTAAGGGTGCAACT
  TACGGCAAGCCTGTCCATCATGGTGTTAACCAGCTAAAGTTTGCTCGAAGCCTTCAGTCC
  GTTGCAGAGGAGCGAGCTGGACGCCACTGTGGGGCTCTGAGAGTCCTGAATTCTTACTGG
  GTTGGTGAAGATTCCACATACAAATTTTTTGAGGTTATCCTCATTGATCCATTCCATAAA
  GCTATCAGAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGAG
  ATGCGTGGGCTGACATCTGCAGGCCGAAAGAGCCGTGGCCTTGGAAAGGGCCACAAGTTC
  CACCACACTATTGGTGGCTCTCGCCGGGCAGCTTGGAGAAGGCGCAATACTCTCCAGCTC
  CACCGTTACCGCTAA
  
  Clone variation with respect to NM_002948.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002948 unedited
  CTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCAGCCATCAGGTAAGCCAAGATGG
  GTGCATACAAGTACATCCAGGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGCGCTTTC
  TTCTGAGGGTCCGCTGCTGGCAGTACCGCCAGCTCTCTGCTCTCCACAGGGCTCCCTGCC
  CCACCCGGCCTGATAAAGCGCGCCGACTGGGCTACAAGGCCAAGCAAGGTTACGTTATAT
  ATAGGATTCGTGTTCGCCGTGGTGGCCGAAAACGCCCAGTTCCTAAGGGTGCAACTTACG
  GCAAGCCTGTCCATCATGGTGTTAACCAGCTAAAGTTTGCTCGAAGCCTTCAGTCCGTTG
  CAGAGGAGCGAGCTGGACGCCACTGTGGGGCTCTGAGAGTCCTGAATTCTTACTGGGTTG
  GTGAAGATTCCACATACAAATTTTTTGAGGTTATCCTCATTGATCCATTCCATAAAGCTA
  TCAGAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGAGATGC
  GTGGGCTGACATCTGCAGGCCGAAAGAGCCGTGGCCTTGGAAAGGGCCACAAGTTCCACC
  ACACTATTGGTGGCTCTCGCCGGGCAGCTTGGAGAAAGCGCAATACTCTCCAGCTNCACC
  GTTACCGCTATATAAGTAAAGTTTGTAAATTCATACTTATAAACATTTANGACAGTCATG
  TCTGCTTACAGGTNGTATTTGTCTGTAAAACTAGTCTGCAGATGTTTCTGAAAATGCTTG
  TCAATAAGAAAGNTAAGTGCANNTATGTTTGAGACCATAGTGGTGGTTGTATCTGGNTCT
  ATAAGAAAACTTTTNTGNCTTGCNTATCTTTTAAGGGANNTGTATGTCAGGATAAACATA
  CTGGGTGGATAACAGGCTNATAATCTTTAAAGGAAGAACTGACAGCCTGGAAATTGGCTG
  GGCAGGGAGAACCGCGCTTTTCGATATGCAGCTCTCGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002948 unedited
  GGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGTTTAATAGACATTTATTCC
  TTCAGTTGAACAACTTCTACACAATTAAAATGTATGACTTAAGATCTTTTCTTTTTTTGT
  GAAGAAATTTAGGTCTCAAGAACTTTTATGAACTTGCTATGAGTACTTCCTGGAAATCAA
  TTAACTGAGTCTTTTGAAACCCCTAGAGAAGATAGGAGAAAATTGGTTCAGAACGAGCAT
  TTAAATTAAGTCAGCAAAGTCAGAATTTAAAATTGGGCAATTCCTTGTCTACATTTTCTT
  TACACTCAAATTGACCCTGGACAACAGTAGTCTAGTCTCCTGGAAGACATGGTGCAAACA
  GAAATGCAATGTGGTGGTATACTCATAGTCAAAGTGGGTGCACAAGTGATGGTCCAACTT
  TGTGGAATGGTAAGGATTTTTAGGGTTGTTTGGCCAGAACAAGAGAAATAACTGCAGAAA
  ACACATATGGTTGGAAACCATGCGCTTGTGACTTTTTCTGTAGCCTATGGGAGTGGACAG
  AGTGGGTAACCCAAGATGTTTTTAAGACTGACTGGACTAAGAATGACGTACTTATAGCCA
  ACTACTTCCCCCCTAATGTGACTGAAGGGATTCATAATGATCACAATTAGCATTACGGTT
  AAGTATTTTAGGGTTGACGTCTAAGCTCACACTTGAAAGGTATTTATCTAATGGCCACTG
  GCTCACCAGCTTCCCACCCTAGCATTCATTGCTAGCTTACCAATCTTAAGGGCTAATATT
  TTATCGCACATTCAACTTCCAAACACACCAGTGTTTACCATTACCACTAATCACTGAATG
  CCATGAAGCCATGCNCACTGACCAGNAGCTTTCACCTTTTATGTGATTTAAAATGGNACT
  GTGGNAGCTGAGGCANTTTCTTCAGCTACCCAGATTTCTAAGCCCACTAAAGTCTACCCT
  GCCACTAAAAACGCAGCCCTGAAATAACACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002948
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002948.2, NP_002939.2
• RefSeq Size: 2018 bp
• RefSeq ORF: 615 bp
• Locus ID: 6138
• Cytogenetics: 3p24.2
• Domains: Ribosomal_L15e
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L15E family of ribosomal proteins and a component of the 60S subunit. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Elevated expression of this gene has been observed in esophageal tumors and gastric cancer tissues, and deletion of this gene has been observed in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1-5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.