RPS27A (NM_002954) Human Untagged Clone

CAT#: SC118296

RPS27A (untagged)-Human ribosomal protein S27a (RPS27A), transcript variant 1


  "NM_002954" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RPS27A
Synonyms CEP80; HEL112; S27A; UBA80; UBC; UBCEP1; UBCEP80
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_002954 edited
GAATTCGGCACGAGGGCGGTGGAGCCGCCACCAAAATGCAGATTTTCGTGAAAACCCTTA
CGGGGAAGACCATCACCCTCGAGGTTGAACCCTCGGATACGATAGAAAATGTAAAGGCCA
AGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGC
AGCTGGAAGATGGACGTACTTTGTCTGACTACAATATTCAAAAGGAGTCTACTCTTCATC
TTGTGTTGAGACTTCGTGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCA
AGAAGAATAAGCACAAGAGAAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATAAGGTGG
ATGAGAATGGCAAAATTAGTCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCTG
GGGTGTTTATGGCAAGTCACTTTGACAGACATTATTGTGGCAAATGTTGTCTGACTTACT
GTTTCAACAAACCAGAAGACAAGTAACTGTATGAGTTAATAAAAGACATGAACTAACAAA
AAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_002954 unedited
GTAAAGTTCATACTTGTAATCGACTCATATAGGCGGCCGCGTAATTCGCACGAGGGCGGT
GGAGCCGCCCCAAATNGCAGATTTTCGTGAAACCCCTTACGGGNGAAGACCATCACCCTC
GAGGTTGAACCCTCGGATACCGATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGG
AATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGCAGCTGGAAGATGGACGTAC
TTTGTCTGACTACAATATTCAAAAGGAGTCTACTCTTCATCTTGTGTTGAGACTTCGTGG
TGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCAAGAAGAATAAGCACAAGAG
AAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATAAGGTGGATGAGAATGGCAAAATTAG
TCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCTGGGGTGTTTATGGCAAGTCA
CTTTGACAGACATTATTGTGGCAAATGTTGTCTGACTTACTGTTTCAACAAACCAGAAGA
CAAGTAACTGTATGAGTTAATAAAAGACATGAACTAACAAAAAAAAAAAAAAAAAACTCG
ACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTG
TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCT
TGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATATGG
GTGAGNGGGGGGGGTGTNTNNNNNACANGGGGGNNNNTTTGGNAAGGACACCGTGGGGGG
CCCTGCGGGGTCTATTGGGAACCAACCTGGAGTGCATTGGCACCATCTTGGCTCACTGCC
ATCTCCGCCTTCTGGGTTCCAGCCGTT
>OriGene 3' read for NM_002954 unedited
AGAGTCGAGTTTTTTTTTTTTTTTTTTGTTAGTTCATGTCTTTTATTAACTCATACAGTT
ACTTGTCTTCTGGTTTGTTGAAACAGTAAGTCAGACAACATTTGCCACAATAATGTCTGT
CAAAGTGACTTGCCATAAACACCCCAGCACCACATTCATCAGAAGGGCACTCTCGACGAA
GGCGACTAATTTTGCCATTCTCATCCACCTTATAATATTTCAGGACAGCCAGCTTAACCT
TCTTTCTCTTGTGCTTATTCTTCTTGGGAGTGGTGTAAGACTTCTTCTTCCTTTTCTTAG
CACCACCACGAAGTCTCAACACAAGATGAAGAGTAGACTCCTTTTGAATATTGTAGTCAG
ACAAAGTACGTCCATCTTCCAGCTGCTTGCCAGCAAAGATCAGTCTCTGCTGATCAGGAG
GAATTCCTTCCTTATCCTGGATCTTGGCCTTTACATTTTCTATCGTATCCGAGGGTTCAA
CCTCGAGGGTGATGGTCTTCCCCGTAAGGGTTTTCACGAAAATCTGCATTTTGGTGGCGG
CTCCACCGCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCT
GACGGTTCACTAAACGAGCTCTGCTTATATAGACCCTCCACCGTACACGCCTACCGNCCA
TTTGCGTCAACGGGGGCGGGGTTATTACGACATTNTGGAAAGTCCNNGTTGATTTGGTGC
CAAAACAAACTCCATTGACGTCAATGGGGTGGAGACTGGNAAATNCCCGTGAGTCAAACC
GCTATCCACGCCCATTGGTGTACTGGCAAAACCGCATCACATGGGTATAGCGATGACTAA
TACGTANATGTACTGCCCAGTANGAAAGTNCCCGTANGGTCATGTACTGGGCATAATGCC
ACGCGGCCCATTTACCGNNCATGACGTCAATGGGGGGGCGGACTNGGCATATGATCACCT
TGATGTACTGCCAGTGGGCAGNNTACCGTATTACTCCCCCATGACGTNATGGGAAGTCTT
ATGGNCGTCTTGGGAAACTACGTANTATGCGNCATGGGGCCGGGGGTTCTTGGCGGCA
Restriction Sites NotI-NotI     
ACCN NM_002954
Insert Size 490 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002954.3, NP_002945.1
RefSeq Size 541 bp
RefSeq ORF 471 bp
Locus ID 6233
Cytogenetics 2p16.1
Domains UBQ, Ribosomal_S27
Protein Families Druggable Genome
Protein Pathways Ribosome
Gene Summary 'Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]'
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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