MRP8 (S100A8) (NM_002964) Human Untagged Clone

CAT#: SC118302

S100A8 (untagged)-Human S100 calcium binding protein A8 (S100A8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S100A8
• Synonyms: 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118302 sequence for NM_002964 edited (data generated by NextGen Sequencing)
  ATGTTGACCGAGCTGGAGAAAGCCTTGAACTCTATCATCGACGTCTACCACAAGTACTCC
  CTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGAAGAAATTGCTAGAGACC
  GAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGGTTCAAAGAGTTGGATATC
  AACACTGATGGTGCAGTTAACTTCCAGGAGTTCCTCATTCTGGTGATAAAGATGGGCGTG
  GCAGCCCACAAAAAAAGCCATGAAGAAAGCCACAAAGAGTAG
  
  Clone variation with respect to NM_002964.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002964 unedited
  ACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAAGACCTGGTGGG
  GCAAGTCCGTGGGCATCATGTTGACCGAGCTGGAGAAAGCCTTGAACTCTATCATCGACG
  TCTACCACAAGTACTCCCTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGA
  AGAAATTGCTAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGGT
  TCAAAGAGTTGGATATCAACACTGATGGTGCAGTTAACTTCCAGGAGTTCCTCATTCTGG
  TGATAAAGATGGGCGTGGCAGCCCACAAAAAAAGCCATGAAGAAAGCCACAAAGAGTAGC
  TGAGTTACTGGGCCCAGAGGCTGGGCCCCTGGACATGTACCTGCAGAATAATAAAGTCAT
  CAATACCTCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTG
  TTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCC
  TGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTT
  TGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGTGGTATGGAGCAGGGG
  CAAGTTGGGAAGACAACCTGTAGGGCCTGCGGNGTCTATTGGGAACCAAGCTGGAGTGCA
  GTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCT
  CAGCCTCCCGAGTTGTTGGGAATTCCAGCATGCATGNACCAGCTCAGCTAATTTTTGGTT
  TTTTGGTAGAAACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCACTCCTAATCTCAGT
  GATCTACCCACCTTGCCTCCAANTGCTGGATTACAGCGTGACACTGCTCCTTCTGTCTCT
  GATTTAAAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002964 unedited
  GGCCCCCGGCCCANACAANNNCCCCTCANNNNNNAAGGTTTTGACTTGNNACCGCGGCCG
  CATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTNGAGTATTGATGACTTTATTATTCTGC
  AGGTACATGTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTTTGTGGCTTT
  CTTCATGGCTTTTTTTGTGGGCTGCCACGCCCATCTTTATCACCAGAATGAGGAACTCCT
  GGAAGTTAACTGCACCATCAGTGTTGATATCCAACTCTTTGAACCAGACGTCTGCACCCT
  TTTTCCTGATATACTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGA
  CGGCATGGAAATTCCCCTTTATCAGGGAGTACTTGTGGTAGACGTCGATGATAGAGTTCA
  AGGCTTTCTCCAGCTCGGTCAACATGATGCCCACGGACTTGCCCCACCAGGTCTTCCTCG
  TGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAA
  CGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGN
  GCGGGGTTATTACGACATTNTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCA
  TTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCA
  TTGGGTGTACTGCCAAACCGCATCACCATGGTAATAGCGATGACTATACGTANATGTACT
  GCCAAGTANGAAAGTCCCCGTAAGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTAC
  CGTCATTGACGTTCATAGGGGGCCGGACTTGGCATATGATACACTCGATGAACTGGCAAG
  GGGGCAGTTTACCGGAAAACCTCCCCCATTGACGGCCATGGAAAAGCCCTTATTGGGTTA
  CTTTGGGAACTACGTACTTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002964
• Insert Size: 450 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002964.3, NP_002955.2
• RefSeq Size: 428 bp
• RefSeq ORF: 282 bp
• Locus ID: 6279
• Cytogenetics: 1q21.3
• Domains: S_100, EFh
• Gene Summary: 'The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (4) uses an alternate splice junction compared to variant 1. The resulting isoform (d) is shorter at the N-terminus compared to isoform a. Variants 4 and 5 both encode the same isoform (d).