SAT1 (NM_002970) Human Untagged Clone

CAT#: SC118307

SAT1 (untagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SAT1
• Synonyms: DC21; KFSD; KFSDX; SAT; SSAT; SSAT-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118307 sequence for NM_002970 edited (data generated by NextGen Sequencing)
  ATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGCCGACTGCAGTGACATACTGCGGCTG
  ATCAAGGAGCTGGCTAAATATGAATACATGGAAGAACAAGTAATCTTAACTGAAAAAGAT
  CTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTACCACTGCCTGGTTGCAGAAGTGCCG
  AAAGAGCACTGGACTCCGGAAGGACACAGCATTGTTGGTTTTGCCATGTACTATTTTACC
  TATGACCCGTGGATTGGCAAGTTATTGTATCTTGAGGACTTCTTCGTGATGAGTGATTAT
  AGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAATCTAAGCCAGGTTGCAATGAGGTGT
  CGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATGGAATGAACCATCCATCAACTTCTAT
  AAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGAGGGTTGGAGACTGTTCAAGATCGAC
  AAGGAGTACTTGCTAAAAATGGCAACAGAGGAGTGA
  
  Clone variation with respect to NM_002970.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002970 unedited
  ATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGTCGCGGGCCGACTGGTGTTTA
  TCCGTCACTCGCCGAGGTTCCTTGGGTCATGGTGCCAGCCTGACTGAGAAGAGGACGCTC
  CCGGGAGACGAATGAGGAACCACCTCCTCCTACTGTTCAAGTACAGGGGCCTGGTCCGCA
  AAGGGAAGAAAAGCAAAAGACGAAAATGGCTAAATTCGTGATCCGCCCAGCCACTGCCGC
  CGACTGCAGTGACATACTGCGGCTGATCAAGGAGCTGGCTAAATATGAATACATGGAAGA
  ACAAGTAATCTTAACTGAAAAAGATCTGCTAGAAGATGGTTTTGGAGAGCACCCCTTTTA
  CCACTGCCTGGTTGCAGAAGTGCCGAAAGAGCACTGGACTCCGGAAGGACACAGCATTGT
  TGGTTTTGCCATGTACTATTTTACCTATGACCCGTGGATTGGCAAGTTATTGTATCTTGA
  GGACTTCTTCGTGATGAGTGATTATAGAGGCTTTGGCATAGGATCAGAAATTCTGAAGAA
  TCTAAGCCAGGTTGCAATGAGGTGTCGCTGCAGCAGCATGCACTTCTTGGTAGCAGAATG
  GAATGAACCATCCATCAACTTCTTTAAAAGAAGAGGTGCTTCTGATCTGTCCAGTGAAGA
  GGGTTGGAGACTGTTCAAGATCGACAGGGAGTACTTGCTAAAAATGGCAACAGAGGAGTG
  AGGAGTGCTGCTGTAGATGACCACCTCCATTCTATTNTAGAAATAATTCCCAACTTCTCT
  TGCTTTCTATGCTGNTTGTAGGGAAATATAGAATGAGCACCCATTCCAAGCTNTATTACC
  AGTGGNCGTGTTGCATGTTGAAATGAGGTCTGTTTAAAGTGGCATCTCAGAAGCAGTTTG
  GAGAGTCAGAACTTTCTTCTTGAATATCTTTNCGATACACAGGGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002970 unedited
  NNNTTTACTCTGGACCGCGGCACGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTAAA
  TCAAANAGTAGTTTATTAAAAAAGAATCAAACAGAAACTCTAAGTACCAGTGTGTACATT
  GTACACATTTAAATGACTCACGAGAATGAAGTTTTTTTCAAATATATTAAGATCACACCA
  CCTTGTTGTTTATCGAAAGATATTCAAGGAGAAAGATCTGACTCTCCAAACTGCATCTGA
  GATTGCCACTTTAAACAGACCTCATTTCAAACATGCAACAACGCCACTGGTAATAAAGCT
  TTGGAATGGGTGCTCATTCTATTATTTCACTACAAACAGCATAGAAAGCAAGAGAAGTTG
  GGAATTTATTCTAAAATAGAATGGAGGTTGTCATCTACAGCAGCACTCCTCACTCCTCTG
  TTGCCATTTTTAGCAAGTACTCCTTGTCGATCTTGAACAGTCTCCAACCCTCTTCACTGG
  ACAGATCAGAAGCACCTCTTCTTTTATAGAAGTTGATGGATGGTTCATTCCATTCTGCTA
  CCAAGAAGTGCATGCTGCTGCAGCGACACCTCATTGCAACCTGGCTTAGATTCTTCAGAA
  TTTCTGATCCTATGCCAAAGCCTCTATAATCACTCATCACGAAGAAGTCCTCAAGATACA
  ATAACTTGCCAATCCACGGGTCATAGGTAAAATAGTACATGGCAAAACCAACAATGCTGT
  GTCCTTCCGGAGTCCAGTGCTCTTTCGGCACTTCTGCACCCAGCAGTGGTAAAGGGGTGC
  TCTCCAAACCATCTTCTAGCAGATCTTTTTCAGTTAAGATACTTGTTCTTCATGTATNAT
  ATTTAGCCAGCTCCTTGACAGCCGCATATGTACTGCATTCGCGGCAGTGCTGGGCGATCA
  CA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002970
• Insert Size: 970 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002970.1, NP_002961.1
• RefSeq Size: 1060 bp
• RefSeq ORF: 516 bp
• Locus ID: 6303
• Cytogenetics: Xp22.11
• Domains: Acetyltransf
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) represents the protein coding transcript.