SATB1 (NM_002971) Human Untagged Clone

CAT#: SC118309

SATB1 (untagged)-Human SATB homeobox 1 (SATB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SATB1
• Synonyms: DNA-binding protein SATB1; SATB homeobox 1; special AT-rich sequence binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118309 sequence for NM_002971 edited (data generated by NextGen Sequencing)
  ATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTG
  AGTGATCCGAAGGGTCCACCAGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCGCTA
  GGAAGAGGAAGGCTTGGGAGTACAGGTGCAAAAATGCAGGGAGTGCCTTTAAAACACTCG
  GGCCATCTGATGAAAACCAACCTTAGGAAAGGAACCATGCTGCCAGTTTTCTGTGTGGTG
  GAACATTATGAAAACGCCATTGAATATGATTGCAAGGAGGAGCATGCAGAATTTGTGCTG
  GTGAGAAAGGATATGCTTTTCAACCAGCTGATCGAAATGGCATTGCTGTCTCTAGGTTAT
  TCACATAGCTCTGCTGCCCAGGCCAAAGGGCTAATCCAGGTTGGAAAGTGGAATCCAGTT
  CCACTGTCTTACGTGACAGATGCCCCTGATGCTACAGTAGCAGATATGCTTCAAGATGTG
  TATCATGTGGTCACATTGAAAATTCAGTTACACAGTTGCCCCAAACTAGAAGACTTGCCT
  CCCGAACAATGGTCGCACACCACAGTGAGGAATGCTCTGAAGGACTTACTGAAAGATATG
  AATCAGAGTTCATTGGCCAAGGAGTGCCCCCTTTCACAGAGTATGATTTCTTCCATTGTG
  AACAGTACTTACTATGCAAATGTCTCAGCAGCAAAATGTCAAGAATTTGGAAGGTGGTAC
  AAACATTTCAAGAAGACAAAAGATATGATGGTTGAAATGGATAGTCTTTCTGAGCTATCC
  CAGCAAGGCGCCAATCATGTCAATTTTGGCCAGCAACCAGTTCCAGGGAACACAGCCGAG
  CAGCCTCCATCCCCTGCGCAGCTCTCCCATGGCAGCCAGCCCTCTGTCCGGACACCTCTT
  CCAAACCTGCACCCTGGGCTCGTATCAACACCTATCAGTCCTCAATTGGTCAACCAGCAG
  CTGGTGATGGCTCAGCTGCTGAACCAGCAGTATGCAGTGAATAGACTTTTAGCCCAGCAG
  TCCTTAAACCAACAATACTTGAACCACCCTCCCCCTGTCAGTAGATCTATGAATAAGCCT
  TTGGAGCAACAGGTTTCGACCAACACAGAGGTGTCTTCCGAAATCTACCAGTGGGTACGC
  GATGAACTGAAACGAGCAGGAATCTCCCAGGCGGTATTTGCACGTGTGGCTTTTAACAGA
  ACTCAGGGCTTGCTTTCAGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCCAG
  TCTTTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTGCAGTTACCGGAAGCTGAAAGA
  GACCGAATATACCAGGACGAAAGGGAAAGGAGCTTGAATGCTGCCTCGGCCATGGGTCCT
  GCCCCACTCATCAGCACACCACCCAGCCGTCCTCCCCAGGTGAAAACAGCTACTATTGCC
  ACTGAAAGGAATGGGAAACCAGAGAACAATACCATGAACATTAATGCTTCCATTTATGAT
  GAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCTCAAGCACTGTTTGCAAAGGTTGCA
  GCAACCAAAAGCCAGGGATGGTTGTGCGAGCTGTTACGCTGGAAAGAAGATCCTTCTCCA
  GAAAACAGAACCCTGTGGGAGAACCTCTCCATGATCCGAAGGTTCCTCAGTCTTCCTCAG
  CCAGAACGTGATGCCATTTATGAACAGGAGAGCAACGCGGTGCATCACCATGGCGACAGG
  CCGCCCCACATTATCCATGTTCCAGCAGAGCAGATTCAGCAACAGCAGCAGCAACAGCAA
  CAGCAGCAGCAGCAGCAGCAGGCACCGCCGCCTCCACAGCCACAGCAGCAGCCACAGACA
  GGCCCTCGGCTCCCCCCACGGCAACCCACGGTGGCCTCTCCAGCAGAGTCAGATGAGGAA
  AACCGACAGAAGACCCGGCCACGAACAAAAATTTCAGTGGAAGCCTTGGGAATCCTCCAG
  AGTTTCATACAAGACGTGGGCCTGTACCCTGACGAAGAGGCCATCCAGACTCTGTCTGCC
  CAGCTCGACCTTCCCAAGTACACCATCATCAAGTTCTTTCAGAACCAGCGGTACTATCTC
  AAGCACCACGGCAAACTGAAGGACAATTCCGGTTTAGAGGTCGATGTGGCAGAATATAAA
  GAAGAGGAGCTGCTGAAGGATTTGGAAGAGAGTGTCCAAGATAAAAATACTAACACCCTT
  TTTTCAGTGAAACTAGAAGAAGAGCTGTCAGTGGAAGGAAACACAGACATTAATACTGAT
  TTGAAAGACTGA
  
  Clone variation with respect to NM_002971.4
  1386 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002971 unedited
  NTTTAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGCCTTCAGG
  TCTGCTGCTTTTTTATTTTTTTTTTTTAATTAAAAAAAAAAGGACATAGAAAACATCAGT
  CTTGAACTTCTCTTCAAGAACCCGGGCTGCAAAGGAAATCTCCTTTGTTTTTGTTATTTA
  TGTGCTGTCAAGTTTTGAAGTGGTGAGTTTCAGGTGATCTTTAGACAGTGACTGAGTATG
  GATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGT
  GATCCGAAGGGTCCACCAGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCGCTAGGA
  AGAGGAAGGCTTGGGAGTACAGGTGCAAAAATGCAGGGAGTGCCTTTAAAACACTCGGGC
  CATCTGATGAAAACCAACCTTAGGAAAGGAACCATGCTGCCAGTTTTCTGTGTGGTGGAA
  CATTATGAAAACGCCATTGAATATGATTGCAAGGAGGAGCATGCAGAATTTGTGCTGGTG
  AGAAAGGATATGCTTTTCAACCAGCTGATCGAAATGGCATTGCTGTCTCTAGGTTATTCA
  CATAGCTCTGCTGCCCAGGCCAAAGGGCTAATCCAGGTTGGAAAGTGGAATCCAGTTCCA
  CTGTCTTACGTGACAGATGCCCCTGATGCTACAGTAGCAGATATGCTTCAAGATGTGTAT
  CATTGTGGTACATTGAAAATTTCAGTACACAGTTGCCCCAACTAGAAAGACTTGCCTNCC
  GACCATGGTCCCACACCACATGAGGAATGCTTGAAGGGACTTACTGAAGAATGNTNCAAA
  GTTCATGCCAAGGAATGGCCCCCTCACAAATATGATTNCTTCATTGGGGAACGGACTACT
  TT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002971 unedited
  GGTATGGCAACTTCCAGGGCCAGNANAGGCACTGGGGAGGGGTCACAGGGATGCCACCCG
  GGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAGGGGCAAGTTTCCA
  AAAATCAGGGGGGAGGGCTCCAAAAATAATTATGGGAGAGGAAATCATAATCCCAAAAGG
  TTTAATGCTTGTTTGAGGCTCCGGAATAAAAACTAAAAAAAAAACAAAAAACACTGGTTT
  CATGCTTACGGGGTACACACTTTGGGGCATCCCGGGAACCCAAATTTTAATACCAAACAA
  TCCTTGATGCTTCACCTGGGGCTGCCAAGCAGTTTGTAAAACAAAGGAAAACATTTAGGG
  CAGTCTGTATTATCCTTTTCCAACTTTTCTGTTTGGGCAAGTTTTTGAAAATTCATTGGC
  CAAACAATGAACAACAAAGGTTTTCTGAAAAAAAACAAGGGGGACTTTTCATTTTGTTAA
  AAAATACCAGGGGCACTGTTAAACAAAACAAATACTTTTATCTCAGTCTTTCAAAACAAT
  ATTAAGGGCTGTGTTTCCTTCCACTGACAGCTCTTCTTTTAGTTTCACTGAAAAAGGGTG
  TTAGTATTTTTATCTTGGACACTCTCTTCCAAATCCTTAACAGCTCCTCTTCTTTATATT
  CTGCCACATTGACCTCTAAACGGGAATTGCCCTTCATTTTGCCCGGGGGCTTGAGAAATA
  CCCCTTGGTTCTGAAAAAACTGATGATGGTGACTTGGGGAAGGTCAACTGGCAAAACAAA
  TTGGAATGCCCTTTCTCAGGTACAGGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002971
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002971.2, NP_002962.1
• RefSeq Size: 3782 bp
• RefSeq ORF: 2292 bp
• Locus ID: 6304
• Cytogenetics: 3p24.3
• Domains: homeobox, CUT
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. [provided by RefSeq, Apr 2016]'
  Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate exon in the 3' coding region, compared to variant 3. Isoform 1 has a shorter C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.