CCL3 (NM_002983) Human Untagged Clone

CAT#: SC118318

CCL3 (untagged)-Human chemokine (C-C motif) ligand 3 (CCL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCL3
• Synonyms: G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118318 sequence for NM_002983 edited (data generated by NextGen Sequencing)
  ATGCAGGTCTCCACTGCTGCCCTTGCTGTCCTCCTCTGCACCATGGCTCTCTGCAACCAG
  TTCTCTGCATCACTTGCTGCTGACACGCCGACCGCCTGCTGCTTCAGCTACACCTCCCGG
  CAGATTCCACAGAATTTCATAGCTGACTACTTTGAGACGAGCAGCCAGTGCTCCAAGCCT
  GGTGTCATCTTCCTAACCAAGCGAAGCCGGCAGGTCTGTGCTGACCCCAGTGAGGAGTGG
  GTCCAGAAATATGTCAGCGACCTGGAGCTGAGTGCCTGA
  
  Clone variation with respect to NM_002983.2
  180 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002983 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGACAGTGG
  TCAGTCCTTTCTTGGCTCTGCTGACACTCGAGCCCACATTCCGTCACCTGCTCAGAATCA
  TGCAGGTCTCCACTGCTGCCCTTGCTGTCCTCCTCTGCACCATGGCTCTCTGCAACCAGT
  TCTCTGCATCACTTGCTGCTGACACGCCGACCGCCTGCTGCTTCAGCTACACCTCCCGGC
  AGATTCCACAGAATTTCATAGCTGACTACTTTGAGACGAGCAGCCAGTGCTCCAAGCCTG
  GTGTCATCTTCCTAACCAAGCGAAGCCGGCAGGTCTGTGCTGACCCCAGTGAGGAGTGGG
  TCCAGAAATATGTCAGCGACCTGGAGCTGAGTGCCTGAGGGGTCCAGAAGCTTCGAGGCC
  CAGCGACCTCGGTGGGCCCAGTGGGGAGGAGCAGGAGCCTGAGCCTTGGGAACATGCGTG
  TGACCTCCACAGCTACCTCTTCTATGGACTGGTTGTTGCCAAACAGCCACACTGTGGGAC
  TCTTCTTAACTTAAATTTTAATTTATTTATACTATTTAGTTTTTGTAATTTATTTTCGAT
  TTCACAGTGTGTTTGTGATTGTTTGCTCTGAGAGTTCCCCTGTCCCCTCCCCGTTCCCTC
  ACACCGCGTCTGGTGACAACCGAGTGGCTGTCATCGGCCTGTGTANGCAGTCATGGCACC
  AAAGCCACCAGACTGACAAATGTGTATCGGATGCTTTTGTTCAGGNCTGTGATNCGNCTG
  NGGAAATAATAAAGATGCTCTTTTAAAAGTANNAAAAAAAAAAAAAACTCGACTCTAGAT
  TGCNNGCCGCGTCATAGCTGNTTCCCTGACAGATCCCNGGTGGCATCCCTGTGACCCCTC
  CCNATGCCTCTCCTGGCCCTGNAAGNTGCACTCCGTGCCCACAGCCCTGTCTATAAAATA
  AGTGCATCATTTGCTGACTAGTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002983 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTACCTTTTAAAAGACATCTTTA
  TTATTTCCCCAGGCCGATCACAGCCCTGAACAAAAGCATCCGATACACATTTGTCAGTCT
  GGTGGCTTTGGTGCCATGACTGCCTACACAGGCCGATGACAGCCACTCGGTTGTCACCAG
  ACGCGGTGTGAGGGAACGGGGAGGGGACAGGGGAACTCTCAGAGCAAACAATCACAAACA
  CACTGTGAAATCGAAAATAAATTACAAAAACTAAATAGTATAAATAAATTAAAATTTAAG
  TTAAGAAGAGTCCCACAGTGTGGCTGTTTGGCAACAACCAGTCCATAGAAGAGGTAGCTG
  TGGAGGTCACACGCATGTTCCCAAGGCTCAGGCTCCTGCTCCTCCCCACTGGGCCCACCG
  AGGTCGCTGGGCCTCGAAGCTTCTGGACCCCTCAGGCACTCAGCTCCAGGTCGCTGACAT
  ATTTCTGGACCCACTCCTCACTGGGGTCAGCACAGACCTGCCGGCTTCGCTTGGTTAGGA
  AGATGACACCAGGCTTGGAGCACTGGCTGCTCGTCTCAAAGTAGTCAGCTATGAAATTCT
  GTGGAATCTGCCGGGAGGTGTAGCTGAAGCAGCAGGCGGTCGGCGTGTCAGCAGCAAGTG
  ATGCAGAGAACTGGTTGCAGAGAGCCATGGTGCAGAGGAGGACAGNAGGGGCAGCAGTGG
  AGACCTGCATGATTCTGAGCAGGTGACGGAATGTGGGCTCGAGTGTCAGCAGAGCCAAGA
  AAGGACTGACCACTGTCTGCCTCGTGCCGAATTCGCGGCCGCCCTTAGTGAGTCGTATAC
  AAAATCTGACGGTTCACTAACGAGCTCTGCTTATTAGACTTCCCCGAACACGCTACCGCC
  CATTGCGTAACCGGCCGGGGTATTACAACATTTGGAAAGTCCGTGATTTTGGGCAAACAA
  CTTCATGCCGTCATGGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002983
• Insert Size: 780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002983.1, NP_002974.1
• RefSeq Size: 776 bp
• RefSeq ORF: 279 bp
• Locus ID: 6348
• Cytogenetics: 17q12
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway
• Gene Summary: 'This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]'