RANGAP1 (NM_002883) Human Untagged Clone

CAT#: SC118326

RANGAP1 (untagged)-Human Ran GTPase activating protein 1 (RANGAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RANGAP1
• Synonyms: Fug1; RANGAP; SD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118326 sequence for NM_002883 edited (data generated by NextGen Sequencing)
  ATGGCCTCGGAAGACATTGCCAAGCTGGCAGAGACACTTGCCAAGACTCAGGTGGCCGGG
  GGACAGCTGAGTTTCAAAGGCAAGAGCCTCAAACTCAACACTGCAGAAGATGCTAAAGAT
  GTGATTAAAGAGATTGAAGACTTTGACAGCTTGGAGGCTCTGCGTCTGGAAGGCAACACA
  GTGGGCGTGGAAGCAGCCAGGGTCATCGCCAAGGCCTTAGAGAAGAAGTCGGAGTTGAAG
  CGCTGCCACTGGAGTGACATGTTCACGGGAAGGCTGCGGACCGAGATCCCACCAGCCCTG
  ATCTCACTAGGGGAAGGACTCATCACAGCTGGGGCTCAGCTGGTGGAGCTGGACTTAAGC
  GACAACGCATTCGGGCCCGACGGTGTGCAAGGCTTCGAGGCCCTGCTCAAGAGCTCAGCC
  TGCTTCACCCTGCAGGAACTCAAGCTCAACAACTGTGGCATGGGCATTGGCGGCGGCAAG
  ATCCTGGCTGCAGCTCTGACCGAATGTCACCGGAAATCCAGTGCCCAAGGCAAGCCTCTG
  GCCCTGAAGGTCTTTGTGGCTGGCAGAAACCGTCTGGAGAATGATGGCGCCACTGCCTTG
  GCAGAAGCTTTTAGGGTCATCGGGACCCTGGAGGAGGTCCACATGCCACAGAATGGGATC
  AACCACCCTGGCATCACTGCCCTGGCCCAGGCTTTCGCTGTCAACCCCCTGCTGCGGGTC
  ATCAACCTGAATGACAACACCTTCACTGAGAAGGGCGCCGTGGCCATGGCCGAGACCTTG
  AAGACCTTGCGGCAGGTGGAGGTGATTAATTTTGGGGACTGCCTGGTGCGCTCCAAGGGT
  GCAGTTGCCATTGCAGATGCCATCCGCGGCGGCCTGCCCAAGCTAAAGGAGCTGAACTTG
  TCATTCTGTGAAATCAAGAGGGATGCTGCCCTGGCTGTTGCTGAGGCCATGGCAGACAAA
  GCTGAGCTGGAGAAGCTGGACCTGAATGGCAACACCCTGGGAGAAGAAGGCTGTGAACAG
  CTTCAGGAGGTGCTGGAGGGCTTCAACATGGCCAAGGTGCTGGCGTCCCTCAGTGATGAC
  GAGGACGAGGAGGAGGAGGAGGAAGGAGAAGAGGAAGAAGAGGAAGCAGAAGAAGAGGAG
  GAGGAAGATGAGGAAGAGGAGGAAGAAGAGGAGGAGGAGGAGGAAGAAGAGCCTCAGCAG
  CGAGGGCAGGGAGAGAAGTCAGCCACGCCCTCACGGAAGATTCTGGACCCTAACACTGGG
  GAGCCAGCTCCCGTGCTGTCCTCCCCACCTCCTGCAGACGTCTCCACCTTCCTGGCTTTT
  CCCTCTCCAGAGAAGCTGCTGCGCCTAGGGCCCAAGAGCTCCGTGCTGATAGCCCAGCAG
  ACTGACACGTCTGACCCCGAGAAGGTGGTCTCTGCCTTCCTAAAGGTGTCATCTGTGTTC
  AAGGACGAAGCTACTGTGAGGATGGCAGTGCAGGATGCAGTAGATGCCCTGATGCAGAAG
  GCTTTCAACTCCTCGTCCTTCAACTCCAACACCTTCCTCACCAGGCTCCTCGTGCACATG
  GGTCTGCTCAAGAGTGAAGACAAGGTCAAGGCCATTGCCAACCTGTACGGCCCCCTGATG
  GCGCTGAACCACATGGTGCAGCAGGACTATTTCCCCAAGGCCCTTGCACCCCTGCTGCTG
  GCGTTCGTGACCAAGCCCAACAGCGCCCTGGAATCCTGCTCCTTCGCCCGCCACAGTCTG
  CTGCAGACGCTGTACAAGGTCTAG
  
  Clone variation with respect to NM_002883.2
  1548 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002883 unedited
  GTTCAGNCATATTTGTNAATACGACTCACTATTAGGGCGGCCGCGNAATTCGCACCAGGC
  TGGATCCGGAGTCTGCAGATCTCCAGGGGGGGCCCACCAGCCTAGTCAACATGGCCTCGG
  AAGACATTGCCAAGCTGGCAGAGACACTTGCCAAGACTCAGGTGGCCGGGGGACAGCTGA
  GTTTCAAAGGCAAGAGCCTCAAACTCAACACTGCAGAAGATGCTAAAGATGTGATTAAAG
  AGATTGAAGACTTTGACAGCTTGGAGGCTCTGCGTCTGGAAGGCAACACAGTGGGCGTGG
  AAGCAGCCAGGGTCATCGCCAAGGCCTTAGAGAAGAAGTCGGAGTTGAAGCGCTGCCACT
  GGAGTGACATGTTCACGGGAAGGCTGCGGACCGAGATCCCACCAGCCCTGATCTCACTAG
  GGGAAGGACTCATCACAGCTGGGGCTCAGCTGGTGGAGCTGGACTTAAGCGACAACGCAT
  TCGGGCCCGACGGTGTGCAAGGCTTCGAGGCCCTGCTCAAGAGCTCAGCCTGCTTCACCC
  TGCAGGAACTCAAGCTCAACAACTGTGGCATGGGCATTGGCGGCGGCAAGATCCTGGCTG
  CAGCTCTGACCGAATGTCACCGGAAATCCAGTGCCCAAGGCAAGCCTCTGGCCCTGAAGG
  TCTTTGTGGCTGGCAGAAACCGTCTGGAGAATGATGGCGCCACTGCCTTGGCAGAAGCTN
  TTAGGGTCATCGGGACCCTGNGAGGAGTCCACATGCCACAGAATGGGATCAACCACCCTG
  GCATCACTGCCCTGGCCCCAGCTTTCGCTGTCAACCCCCTGCTGCGGNTCATCAACCTGA
  ATGANCACACCTCACTGAAAAGGGCGCCGTGGCCATGGCCGAGACCTTGAAGAACTTGCN
  GCAGGTGGAGGTGATTAATTNTTGGGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002883 unedited
  NNNNGGGTTATACTAAGNAACCGCGGCCGCAANCTAGNGATCGATTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACCGC
  CCCCCCACTTTTTTTTGGAAACCCCCGGGGCCCGCCCAAACCCCCCCCGAGCACAAAGGG
  GAAAACCTGGGGCAATCCCCGGGGCCCAGGGGGGGCAAAAAACATTTGCCCAGTCCAAAA
  CCTACCGCTCCCCTGGCCCAAGAGGGCCTTTAAAAACCACGTCCAAAAGCCCCTCGGACA
  TTGGGGACCCCGCCCCTCCCCCCCCAAAATGGAAAAAAACTTTGGGTTGTTAAAACTCCC
  CCCCCAGCCCGGCCAGTTACAAAAAAAATTTTAACAGCCCAGAAAAAGCCCCCTAAGTCC
  GGGGCCCACGCAATCCCAACAACTCCTGGTCCTGGGAAAATTGGGGGCTGCCCGTCCACC
  CCGGGGGGGGCCCCAGGGTGCCCTTTGGCAAAAACCCCCCGATTCCACCCCCACAATCCC
  CCCCCTGGGATTTTCTGGTTTTTAAGGTGAACGGGCAACACAATGGTTTTGATGGGAAGG
  GGGAAAACTTCCCCAAGAACCCGGCTTTGTGACCAAAAACAACCCCCGGCCGGGGGGGGA
  ACAACCCCATCCCACACAAAGGGGAAGGGCCACCCCGCGGGCCACTCCTTGGGTTCTGCC
  TCCCCCCCGGGACCCGGCCCCAAAGCCAGTGGGGGGGGAACCGCCCCTCAGGCCCCAGCC
  GGAAATTAACAAAACCCCCCCCGGGGGGGGGGAAATTAAAGGGGGGAAACCTTCAAACCC
  TTTCCCGGGGGGCCAACCCCCCAAAAAAAAAGGGTGGGCCCACAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002883
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002883.2, NP_002874.1
• RefSeq Size: 3040 bp
• RefSeq ORF: 1764 bp
• Locus ID: 5905
• Cytogenetics: 22q13.2
• Domains: LRR, LRR_RI
• Gene Summary: 'This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.