Arginyl tRNA synthetase (RARS) (NM_002887) Human Untagged Clone

CAT#: SC118328

RARS (untagged)-Human arginyl-tRNA synthetase (RARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RARS
• Synonyms: ArgRS; DALRD1; HLD9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118328 sequence for NM_002887 edited (data generated by NextGen Sequencing)
  ATGGACGTACTGGTGTCTGAGTGCTCCGCGCGGCTGCTGCAGCAGGAAGAAGAGATTAAA
  TCTCTGACTGCTGAAATTGACCGGTTGAAAAACTGTGGCTGTTTAGGAGCTTCTCCAAAT
  TTGGAGCAGTTACAAGAAGAAAATTTAAAATTAAAGTATCGACTGAATATTCTTCGAAAG
  AGTCTTCAGGCAGAAAGGAACAAACCAACTAAAAATATGATTAACATTATTAGCCGCCTA
  CAAGAGGTCTTTGGTCATGCAATTAAGGCTGCATATCCAGATTTGGAAAATCCTCCTCTG
  CTAGTGACACCAAGTCAGCAGGCCAAGTTTGGGGACTATCAGTGTAATAGTGCTATGGGT
  ATTTCTCAGATGCTCAAAACCAAGGAACAGAAAGTTAATCCAAGAGAAATTGCTGAAAAC
  ATTACCAAACACCTCCCAGACAATGAATGTATTGAAAAAGTTGAAATTGCTGGTCCTGGT
  TTTATTAATGTCCACTTAAGAAAGGATTTTGTATCAGAACAATTGACCAGTCTTCTAGTG
  AATGGAGTTCAACTACCTGCTCTGGGAGAGAATAAAAAGGTTATAGTTGACTTTTCCTCC
  CCTAATATAGCTAAAGAGATGCATGTAGGCCACCTGAGGTCAACTATCATAGGAGAGAGT
  ATAAGCCGCCTCTTTGAATTTGCAGGGTATGACGTGCTCAGGTTAAATCATGTAGGAGAC
  TGGGGGACCCAGTTTGGCATGCTCATCGCTCACCTGCAAGACAAATTTCCAGATTATCTA
  ACAGTTTCACCTCCTATTGGGGATCTTCAGGTCTTTTATAAGGAATCTAAGAAGAGGTTT
  GATACTGAGGAGGAATTTAAGAAGCGAGCATATCAGTGTGTAGTTCTGCTCCAGGGTAAA
  AACCCAGATATTACAAAAGCTTGGAAGCTTATCTGTGATGTCTCCCGCCAAGAGTTAAAT
  AAAATCTATGATGCATTGGACGTCTCTTTAATAGAGAGAGGGGAATCCTTCTATCAAGAT
  AGGATGAATGATATTGTAAAGGAATTTGAAGATAGAGGATTTGTGCAGGTGGATGATGGC
  AGAAAGATTGTATTTGTCCCAGGGTGTTCCATACCATTAACCATAGTAAAATCAGATGGA
  GGTTATACCTATGATACATCTGACCTGGCTGCTATTAAACAAAGACTATTTGAGGAAAAA
  GCAGATATGATTATCTATGTTGTGGACAATGGACAATCTGTGCACTTCCAGACAATATTT
  GCTGCTGCTCAAATGATTGGTTGGTATGACCCTAAAGTAACTCGAGTCTTCCATGCTGGA
  TTTGGTGTGGTGCTAGGGGAAGACAAGAAAAAGTTTAAAACACGTTCGGGTGAAACAGTG
  CGCCTCATGGATCTTCTGGGAGAAGGACTAAAACGATCCATGGACAAGTTGAAGGAAAAA
  GAAAGAGACAAGGTCTTAACTGCAGAGGAATTGAATGCTGCTCAGACATCCGTTGCGTAT
  GGCTGCATCAAATATGCTGACCTTTCCCATAACCGGTTGAATGACTACATCTTCTCCTTT
  GACAAAATGCTAGATGACAGAGGAAATACAGCTGCTTACTTGTTGTATGCCTTCACTAGA
  ATCAGGTCTATTGCACGTCTGGCCAATATTGATGAAGAAATGCTCCAAAAAGCTGCTCGA
  GAAACCAAGATTCTTTTGGATCATGAGAAGGAATGGAAACTAGGCCGGTGCATTTTACGG
  TTCCCTGAGATTCTGCAAAAGATTTTAGATGACTTATTTCTCCACACTCTCTGTGATTAT
  ATATATGAGCTGGCAACTGCTTTCACAGAGTTCTATGATAGCTGCTACTGTGTGGAGAAA
  GATAGACAGACTGGAAAAATATTGAAGGTGAACATGTGGCGTATGCTGCTATGTGAAGCA
  GTAGCTGCTGTCATGGCCAAGGGGTTTGATATCCTGGGAATAAAACCTGTCCAAAGGATG
  TAA
  
  Clone variation with respect to NM_002887.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002887 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGACGCTGATGGGAGGA
  TGGACGTACTGGTGTCTGAGTGCTCCGCGCGGCTGCTGCAGCAGGAAGAAGAGATTAAAT
  CTCTGACTGCTGAAATTGACCGGTTGAAAAACTGTGGCTGTTTAGGAGCTTCTCCAAATT
  TGGAGCAGTTACAAGAAGAAAATTTAAAATTAAAGTATCGACTGAATATTCTTCGAAAGA
  GTCTTCAGGCAGAAAGGAACAAACCAACTAAAAATATGATTAACATTATTAGCCGCCTAC
  AAGAGGTCTTTGGTCATGCAATTAAGGCTGCATATCCAGATTTGGAAAATCCTCCTCTGC
  TAGTGACACCAAGTCAGCAGGCCAAGTTTGGGGACTATCAGTGTAATAGTGCTATGGGTA
  TTTCTCAGATGCTCAAAACCAAGGAACAGAAAGTTAATCCAAGAGAAATTGCTGAAAACA
  TTACCAAACACCTCCCAGACAATGAATGTATTGAAAAAGTTGAAATTGCTGGTCCTGGTT
  TTATTAATGTCCACTTAAGAAAGGATTTTGTATCAGAACAATTGACCAGTCTTCTAGTGA
  ATGGAGTTCAACTACCTGCTCTGGGAGAGAATAAAAAGGTTATAGTTGACTTTTCCTCCC
  CTAATATAGCTAAAGAGATGCATGGTAGGCCACCTGAGTCAACTATCATAGGAGAGAGTA
  TAAGCCGCCTCTTTGAATTGCAGGGTATGACGTGCTCANGTTAAATCATGTANGAGACTG
  GGGNACCCAGTTNGNCATGCTCATCGCTCACCTGCAGAAACAAATTTCAGATTATCTACA
  GTTTCACCTCCTATTGGGGATCCTCAAGTCTTTTTATAGGATCTAANNAGAGGTTTGTAC
  TNGAGAGGAATTTAAGAGCGAGCTATCATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002887 unedited
  GGCCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGTTGACAAATTTTCTTTACT
  TATGCTTGTGTCCACATGATTGTAAAAAAGCAAACAGTGCCAATGGCCACTTTGGTAAAA
  ACACACAGTGTTCAAACCTATGAAGGATTACATCCTTTGGACAGGTTTTATTCCCAGGAT
  ATCAAACCCCTTGGCCATGACAGCAGCTACTGCTTCACATAGCAGCATACGCCACATGTT
  CACCTTCAATATTTTTCCAGTCTGTCTATCTTTCTCCACACAGTAGCAGCTATCATAGAA
  CTCTGTGAAAGCAGTTGCCAGCTCATATATATAATCACAGAGAGTGTGGAGAAATAAGTC
  ATCTAAAATCTTTTGCAGAATCTCAGGGAACCGTAAAATGCACCGGCCTAGTTTCCATTC
  CTTCTCATGATCCAAAAGAATCTTGGTTTCTCGAGCAGCTTTTTGGAGCATTTCTTCATC
  AATATTGGCCAGACGTGCAATAGACCTGATTCTAGTGAAGGCATACAACAAGTAAGCAGC
  TGTATTTCCTCTGTCATCTAGCATTTTGTCAAAGGAGAAGATGTAGTCATTCAACCGGTT
  ATGGGAAAGGTCAGCATATTTGATGCAGCCATACGCAACGGATGTCTGAGCAGCATTCAA
  TTCCTCTGCAGTTAAGACCTTGTCTCTTTCTTTNTCCTTCAACTTGTCCATGGATCGTTN
  TAGTCCTTCTCCCAGAAGACCATGAGGCGCACTGTNTCACCCGAACGTGTTTTAAACTTT
  TTCTTGTCTTCCCTAGCCCACACCAATCCAGCATGGAAGATCGAGTACTTTAGGGTCTAC
  CACCCATCATTTGAGCACAGCAAATATGGCTGGAGTGCCAGATTGTCCATGTCACAATAG
  AAACATATCGCTTTTCTCAATAGNCTTGTTTATAGCAGCAGTCAGAGTTCATAGTTTACC
  TCTCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002887
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002887.3, NP_002878.2
• RefSeq Size: 2154 bp
• RefSeq ORF: 1983 bp
• Locus ID: 5917
• Cytogenetics: 5q34
• Domains: tRNA-synt_1d, N-Arg
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: 'Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]'