RCN2 (NM_002902) Human Untagged Clone

CAT#: SC118340

RCN2 (untagged)-Human reticulocalbin 2, EF-hand calcium binding domain (RCN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RCN2
• Synonyms: E6BP; ERC-55; ERC55; TCBP49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118340 sequence for NM_002902 edited (data generated by NextGen Sequencing)
  ATGCGGCTGGGCCCGAGGACCGCGGCGTTGGGGCTRCTGCTGCTGTGCGCCGCCGCGGCC
  GGCGCCGGCAAGGCCGAGGAGCTGCACTACCCGCTGGGCGAGCGCCGCAGCGACTACGAC
  CGCGAGGCGCTGCTGGGCGTCCAGGAAGATGTGGATGAATATGTTAAACTCGGCCACGAA
  GAGCAGCAAAAAAGACTGCAGGCGATCATAAAGAAAATCGACTTGGACTCAGATGGCTTT
  CTCACTGAAAGTGAACTCAGTTCATGGATTCAGATGTCTTTTAAGCATTATGCTATGCAA
  GAAGCAAAACAACAGTTTGTTGAATATGATAAAAACAGTGATGATACTGTGACTTGGGAT
  GAATATAACATTCAGATGTATGATCGTGTGATTGACTTTGATGAGAACACTGCTCTGGAT
  GATGCAGAAGAGGAGTCCTTTAGGAAGCTTCACTTAAAGGACAAGAAGCGATTTGAAAAA
  GCTAACCAGGATTCAGGTCCCGGTTTGAGTCTTGAAGAATTTATTGCTTTTGAGCATCCT
  GAAGAAGTTGATTATATGACGGAATTTGTCATTCAAGAAGCTTTAGAAGAACATGACAAA
  AATGGTGATGGATTTGTTAGTTTGGAAGAATTTCTTGGTGATTACAGGTGGGATCCAACT
  GCAAATGAAGATCCAGAATGGATACTTGTTGAGAAAGACAGATTCGTGAATGATTATGAC
  AAAGATAACGATGGCAGGCTTGATCCCCAAGAGCTGTTACCTTGGGTAGTACCTAATAAT
  CAGGGCATTGCACAAGAGGAGGCGCTTCATCTAATTGATGAAATGGATTTGAATGGTGAC
  AAAAAGCTCTCTGAAGAAGAGATTCTGGAAAACCCGGACTTGTTTCTCACCAGTGAAGCC
  ACAGATTATGGCAGACAGCTCCATGATGACTATTTCTATCATGATGAGCTTTAA
  
  Clone variation with respect to NM_002902.2
  36 g=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002902 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCG
  GCACGAGGTCGCAGCCGGCCCGGGCCCCCGCCAGCCTCCCTCCTCGCGTCCCTCGGTGTC
  CTCCGCGGGCCGGCGCGATGCGGCTGGGCCCGAGGACCGCGGCGTTGGGGCTGCTGCTGC
  TGTGCGCCGCCGCGGCCGGCGCCGGCAAGGCCGAGGAGCTGCACTACCCGCTGGGCGAGC
  GCCGCAGCGACTACGACCGCGAGGCGCTGCTGGGCGTCCAGGAAGATGTGGATGAATATG
  TTAAACTCGGCCACGAAGAGCAGCAAAAAAGACTGCAGGCGATCATAAAGAAAATCGACT
  TGGACTCAGATGGCTTTCTCACTGAAAGTGAACTCAGTTCATGGATTCAGATGTCTTTTA
  AGCATTATGCTATGCAAGAAGCAAAACAACAGTTTGTTGAATATGATAAAAACAGTGATG
  ATACTGTGACTTGGGATGAATATAACATTCAGATGTATGATCGTGTGATTGACTTTGATG
  AGAACACTGCTCTGGATGATGCAGAAGAGGAGTCCTTTAGGAAGCTTCACTTAAAGGACA
  AGAAGCGATTTGAAAAAGCTAACCAGGATTCAGGTCCCGGTTTGAGTCTTGAAGAATTTA
  TTGCTTTTGAGCATCCTGAAGAAAGTGATTATATGACGGAATTTGTCATTCAAGAAGCTN
  TAGAAGAACATGACANANATGGTGATGGATTTTGTTAGTTTGGAAGAATTTCTTGGTGAT
  TACAGGTGGGATCCCACTGCAAATGNAGATCCAGAATGGGATACTTGNTGAGAAAGACAG
  ATTCGTGAATGATTATGACCAAGAAACGATGGCAGGCTTGATCCCCAGAGCTGTACCCTT
  GGGTAGACCCTATATCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002902 unedited
  TGGACCGCGNGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTCAGGGGCACATTCAATTATTTTATAACATGATTCTTTTATTTAAAAAAAAA
  ATAAAAAAATGCAGCAAGAATAATTTGACAGTTTTGGGAACTTTCCACATAAAGGAATGG
  ATGAGGCAAAATAATGTACAATACCACAAAACCTATGCTAAATTCAATATTTATCTGAAA
  GTTAAAAAAAAGCAAACAAACTTTTCCAAAGGACTTTTCATTTTTAATCACTCAAAAATG
  AAAAAGGTTTGGACAATGTCAAATTCCACTCACCATAAGGCTATAGTTATACATTAAATA
  TAATTACTATCTATACATATGCAAAAATATAAAGTTCTATTTCATACAATAAAACCCTTT
  ATAAAAACCATTTTAAAATTAACCAAAACTTCTCAACATTAATATGGGAGGTCTAAGTCC
  TTCTAAAGGTTTCTTTAAAGGTTTTTAAACAAAATGCTAAACCTAAAAACATTGTCCTGT
  CAGTTCCCAAATTAAATCTACTTAAAACAAAAACAAAAATTTATAGCTCGGCCACATACT
  ACTTAAATAATATTGTTCAGGCATCTCTAAAATCCCTCCATGTTTTCAAGTATGGAAATA
  GAACTCAAATATTCCACATTACAGTACTAAACAGATGGAGTATTTAGGAAAGACTTTTGT
  GTCATATGGCACAATATAAATATTTTGTTGCTTCAATACGTTTTGAAATAAATATCAAAT
  TTTGTTTTTTTTTCCTCAAAAACCAAAATTTAATCTACCTTAAAAATATTCTGACTGGGG
  TTAAGACTTAGAGTGGAAAACCACCTCATTTTTTATCACAAAGTTAAGCTGTAACCAATT
  AAAAAGGAGCCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002902
• Insert Size: 2010 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002902.1, NP_002893.1
• RefSeq Size: 1700 bp
• RefSeq ORF: 954 bp
• Locus ID: 5955
• Cytogenetics: 15q24.3
• Domains: EFh
• Gene Summary: 'The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]'
  Transcript Variant: This variant (1) is a predominant transcript and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.