UPF1 (NM_002911) Human Untagged Clone

CAT#: SC118343

UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UPF1
• Synonyms: HUPF1; NORF1; pNORF1; RENT1; smg-2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118343 sequence for NM_002911 edited (data generated by NextGen Sequencing)
  ATGAGCGTGGAGGCGTACGGGCCCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAG
  GCCGAGCTGCTTGGCGCCGACACACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTT
  CCTAGCCAGACGCAGACGCCCCCCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGA
  GGCCCGGGCGGCGCGGGCGCGGGCGCTGCGGCGGGACAGCTCGACGCGCAGGTTGGGCCC
  GAAGGCATCCTGCAGAACGGGGCTGTGGACGACAGTGTAGCCAAGACCAGCCAGTTGTTG
  GCTGAGTTGAACTTCGAGGAAGATGAAGAAGACACCTATTACACGAAGGACCTCCCCATA
  CACGCCTGCAGTTACTGTGGAATACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGC
  AAGAAGTGGTTCTGCAACGGACGTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTT
  GTGAGGGCAAAATGCAAAGAGGTGACCCTGCACAAGGACGGGCCCCTGGGGGAGACAGTC
  CTGGAGTGCTACAACTGCGGCTGTCGCAACGTCTTCCTCCTCGGCTTCATCCCGGCCAAA
  GCTGACTCAGTGGTGGTGCTGCTGTGCAGGCAGCCCTGTGCCAGCCAGAGCAGCCTCAAG
  GACATCAACTGGGACAGCTCGCAGTGGCAGCCGCTGATCCAGGACCGCTGCTTCCTGTCC
  TGGCTGGTCAAGATCCCCTCCGAGCAGGAGCAGCTGCGGGCACGCCAGATCACGGCACAG
  CAGATCAACAAGCTGGAGGAGCTGTGGAAGGAAAACCCTTCTGCCACGCTGGAGGACCTG
  GAGAAGCCGGGGGTGGACGAGGAGCCGCAGCATGTCCTCCTGCGGTACGAGGACGCCTAC
  CAGTACCAGAACATATTCGGGCCCCTGGTCAAGCTGGAGGCCGACTACGACAAGAAGCTG
  AAGGAGTCCCAGACTCAAGATAACATCACTGTCAGGTGGGACCTGGGCCTTAACAAGAAG
  AGAATCGCCTACTTCACTTTGCCCAAGACTGACTCTGACATGCGGCTCATGCAGGGGGAT
  GAGATATGCCTGCGGTACAAAGGGGACCTTGCGCCCCTGTGGAAAGGGATCGGCCACGTC
  ATCAAGGTCCCTGATAATTATGGCGATGAGATCGCCATTGAGCTGCGGAGCAGCGTGGGT
  GCACCTGTGGAGGTGACTCACAACTTCCAGGTGGATTTTGTGTGGAAGTCGACCTCCTTT
  GACAGGATGCAGAGCGCATTGAAAACGTTTGCCGTGGATGAGACCTCGGTGTCTGGCTAC
  ATCTACCACAAGCTGTTGGGCCACGAGGTGGAGGACGTAATCATCAAGTGCCAGCTGCCC
  AAGCGCTTCACGGCGCAGGGCCTCCCCGACCTCAACCACTCCCAGGTTTATGCCGTGAAG
  ACTGTGCTGCAAAGACCACTGAGCCTGATCCAGGGCCCGCCAGGCACGGGGAAGACGGTG
  ACGTCGGCCACCATCGTCTACCACCTGGCCCGGCAAGGCAACGGGCCGGTGCTGGTGTGT
  GCTCCGAGCAACATCGCCGTGGACCAGCTAACGGAGAAGATCCACCAGACGGGGCTAAAG
  GTCGTGCGCCTCTGCGCCAAGAGCCGTGAGGCCATCGACTCCCCGGTGTCTTTTCTGGCC
  CTGCACAACCAGATCAGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTG
  AAAGACGAGACTGGGGAGCTGTCGTCTGCCGACGAGAAGCGGTACCGGGCCTTGAAGCGC
  ACCGCAGAGAGAGAGCTGCTGATGAACGCAGATGTCATCTGCTGCACATGTGTGGGCGCC
  GGTGACCCGAGGCTGGCCAAGATGCAGTTCCGCTCCATTTTAATCGACGAAAGCACCCAG
  GCCACCGAGCCGGAGTGCATGGTTCCCGTGGTCCTCGGGGCCAAGCAGCTGATCCTTGTA
  GGCGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCGGCCAAGGCCGGGCTG
  TCACAGTCGCTCTTCGAGCGCCTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTC
  CAGTACCGGATGCACCCTGCACTCAGCGCCTTCCCATCCAACATCTTCTACGAGGGCTCC
  CTCCAGAATGGTGTCACTGCAGCGGATCGTGTGAAGAAGGGATTTGACTTCCAGTGGCCC
  CAACCCGATAAACCGATGTTCTTCTACGTGACCCAGGGCCAAGAGGAGATTGCCAGCTCG
  GGCACCTCCTACCTGAACAGGACCGAGGCTGCGAACGTGGAGAAGATCACCACGAAGTTG
  CTGAAGGCAGGCGCCAAGCCGGACCAGATTGGCATCATCACGCCCTACGAGGGCCAGCGC
  TCCTACCTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACCAAGCTCTACCAGGAG
  GTGGAGATCGCCAGTGTGGACGCCTTTCAGGGACGCGAGAAGGACTTCATCATCCTGTCC
  TGTGTGCGGGCCAACGAGCACCAAGGCATTGGCTTTTTAAATGACCCCAGGCGTCTGAAC
  GTGGCCCTGACCAGAGCAAGGTATGGCGTCATCATTGTGGGCAACCCGAAGGCACTATCA
  AAGCAGCCGCTCTGGAACCACCTGCTGAACTACTATAAGGAGCAGAAGGTGCTGGTGGAG
  GGGCCGCTCAACAACCTGCGTGAGAGCCTCATGCAGTTCAGCAAGCCACGGAAGCTGGTC
  AACACTATCAACCCGGGAGCCCGCTTCATGACCACAGCCATGTATGATGCCCGGGAGGCC
  ATCATCCCAGGCTCCGTCTATGATCGGAGCAGCCAGGGCCGGCCTTCCAGCATGTACTTC
  CAGACCCATGACCAGATTGGCATGATCAGTGCCGGCCCTAGCCACGTGGCTGCCATGAAC
  ATTCCCATCCCCTTCAACCTGGTCATGCCACCCATGCCACCGCCTGGCTATTTTGGACAA
  GCCAACGGGCCTGCTGCAGGGCGAGGCACCCCGAAAGGCAAGACTGGTCGTGGGGGACGC
  CAGAAGAACCGCTTTGGGCTTCCTGGACCCAGCCAGACTAACCTCCCCAACAGCCAAGCC
  AGCCAGGATGTGGCGTCACAGCCCTTCTCTCAGGGCGCCCTGACGCAGGGCTACATCTCC
  ATGAGCCAGCCTTCCCAGATGAGCCAGCCCGGCCTCTCCCAGCCGGAGCTGTCCCAGGAC
  AGTTACCTTGGTGACGAGTTTAAATCACAAATCGACGTGGCGCTCTCACAGGACTCCACG
  TACCAGGGAGAGCGGGCTTACCAGCATGGCGGGGTGACGGGGCTGTCCCAGTATTAA
  
  Clone variation with respect to NM_002911.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002911 unedited
  CCGCGGAATTCGCACGAGGCCGGGGCGGGCGGTTTGGTCCTTTCCGGGCGCGCGGGGGCG
  ACAGCGGCAGCGACCCGAGGCCTGCGGCCTAGGCCTCAGCGCGGCGGCGGGCTCGAGTGC
  AGCGCGGAACCGGCCCGAGGGCCCTACCCGGAGGCACCATGAGCGTGGAGGCGTACGGGC
  CCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTGCTTGGCGCCGACA
  CACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACGCCCC
  CCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGG
  GCGCTGCGGCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGG
  CTGTGGACGACAGTGTAGCCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAG
  ATGAAGAAGACACCTATTACACGAAGGACCTCCCCATACACGCCTGCAGTTACTGTGGAA
  TACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGCAACGGAC
  GTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTTGTGAGGGCAAAATGCAAAGAGG
  TGACCCTGCACAAGGACGGGGCCCCTGGGGGAGACAGTCCCTGGAGTGCTACAACTGGCG
  GCTGTCGCAACGTCTTTCTCCTCGGCTTCATCCCGGGCAAAGCTGACTCAATGGGTGGGG
  GCTGCTGTGCAGGCAACCCCTGTGCCAACCCGAGCAGCCTCAAAGGACATAACTGGGACA
  ACTCGCAGTGGCAGACCGGTGATCCAGGAACGCGGGTTCCTGTCCTGGGTTGGGCAAAGA
  ACCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002911 unedited
  AACGTAGGGGAAACAGGTATCCGTTTTTTTTTATTTTTTATTACCCAGNATAAGATGCTG
  ATGGACTAAGCCACGTTGCTTAGCTCTTCCGCCGCCACCTTTTAATACTGGGACAGCCCC
  GTCACCCCGCCATGCTGGTAAGCCCGCTCTCCCTGGTACGTGGAGTCCTGTGAGAGCGCC
  ACGTCGATTTGTGATTTAAACTCGTCACCAAGGTAACTGTCCTGGGACAGCTCCGGCTGG
  GAGAGGCCGGGCTGGCTCATCTGGGAAGGCTGGCTCATGGAGATGTAGCCCTGCGTCAGG
  GCGCCCTGAGAGAAGGGCTGTGACGCCACATCCTGGCTGGCTTGGCTGTTGGGGAGGTTA
  GTCTGGCTGGGTCCAGGAAGCCCAAAGCGGTTCTTCTGGCGTCCCCCACGACCAGTCTTG
  CCTTTCGGGGTGCCTCGCCCTGCAGCAGGCCCGTTGGCTTGTCCAAAATAGCCAGGCGGT
  GGCATGGGTGGCATGACCAGGTTGAAGGGGATGGGAATGTTCATGGCAGCCACGTGGCTA
  GGGCCGGCACTGATCATGCCAATCTGGTCATGGGTCTGGAAGTACATGCTGGAAGGCCGG
  CCCTGGCTGCTCCGATCATAGACGGAGCCTGGGATGATGGCCTCCCGGGCATCATACATG
  GCTGTGGTCATGAAGCGGGCTCCCGGTTGATAGTGTTGACCAGCTTCCGTGGCTTGCTGA
  ACTGCATGAGGCTTTCACCGCAGTTGTTGAGCGGGCCCTTCAACAGCACCCTTTTGTTCC
  TTATAGTAGTTCAGCAGGTGGGTTTCAGAGCCGGCTGCTTTGTTAAGTGCTCTCGGTTGC
  CCCCAATGATGAACCCCTAACCTTGCTCTGGTCAGGGCCACCCTCAACCCCCTTGGGTTA
  TTTTAAAAAACCATGGCTCTGGGCTTTCTTGCCCCACAACGGGAAGGT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002911
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002911.2, NP_002902.2
• RefSeq Size: 5300 bp
• RefSeq ORF: 3357 bp
• Locus ID: 5976
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.