RFC1 (NM_002913) Human Untagged Clone

CAT#: SC118344

RFC1 (untagged)-Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1


  "NM_002913" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RFC1
Synonyms A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002913, the custom clone sequence may differ by one or more nucleotides


ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGA
ATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAATAG
CTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCAGAT
TCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCTTCTA
AACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGACTTTAT
GTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAAC
ATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACAT
CAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGAAA
AGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTACAGCTTGAT
GAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCCATGTTGGATG
AAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCTGTCCAAGCCAA
TTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCAGATGAAAGAAAG
AGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACATTCCAAGTCATCAG
CTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATGAAAAGAAAAGAAGA
GAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATTAAATTGAAAGGAGAG
ACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGTCCTGAAGATTCTGAAA
AGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAGGCTCTGGGCTCCAA
AGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATCACAGGCGTGCTGGAGTCT
ATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTAACAGGAAATGTCAGCAAGA
AAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGAC
AAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCAGGCAAGAAATCCAAGTATGAA
ATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACACCCCAAAAAAATGTCCAAGGAA
AAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGCAGGCCGACTTCCAAAAGGGACAG
TTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGCCTGGATTTCAAGGAGCAGGTGGCT
GAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGACAGCAGTGAAAACAAAGTGGAAAATT
TGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATAATTGGACAGCAAGGTGACCAGAGCTG
TGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGTTCTTCCGAAGATAAAAAACACGCAAAG
TTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTTAAAGCAGCGTTGCTGTCAGGCCCTCCTGGTG
TTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAGTTGGGATACAGCTACGTGGAACTGAATGCAAG
TGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTTGCTGAGTCACTGAACAATACCAGCATCAAAGGC
TTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACGAAACATGCTCTCATCATGGATGAAGTAGATGGCA
TGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAATTAATTGGCCTGATAAAACATACTAAAATTCCCAT
TATTTGTATGTGCAATGATAGAAATCATCCCAAGATTCGCTCTCTGGTTCATTATTGTTTTGATCTTCGT
TTTCAAAGACCTCGGGTTGAACAGATTAAGGGTGCTATGATGTCTATTGCATTTAAAGAAGGTTTAAAGA
TTCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCCAATCAAGATATCAGACAGGTTTTACATAATCT
GAGTATGTGGTGTGCACGAAGTAAAGCATTAACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCCAAA
AAGGATATCAAAATGGGCCCATTTGATGTTGCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTCACA
TGTCACTTGTGGACAAGTCAGATCTCTTTTTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGAAAA
TTACATACACGTGAAGCCTGTAGCAGCAGGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGAGCA
GCAGACAGCATATGCGATGGTGACCTAGTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTCTGC
CTGCGCAGGCCATTTATGCCAGTGTTCTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCCCAC
CTTCCCAAGCTGGCTGGGGAAGCACTCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCCTTG
CATATGAGTCTCAGAACTTACTCCAGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGGATG
CACTTGTACAGCCCTTGACCTCACAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACACATA
TTATTTGATGAAAGAAGACTTTGAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCCTTT
TCAAAGCTGGATCCCAAGGTGAAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTCCAT
ACTCACTTCAAGCTATAAAGGCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGAAGA
ATTAAATGAAGATGACTCTCAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATCAAG
AAAAAGACAAAATCTTCAAAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAAAAA
GTTCGAAGAAATGA


>OriGene 5' read for NM_002913 unedited
GGGGTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTGCACT
TCGCGGGAGAAGTTGTTGGCGCGAATGGATCCTGAGCCTCGATAACAGATTCCTCAACCG
GCCCACCCGCCAGCCAGCCAGCGCCTTCATCCTGGGGCTGCGATGGACATTCGGAAATTC
TTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGAATGAGAAA
ACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAAT
AGCTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATC
TATGATTCAGATTCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCA
GAAAAACTGCCAGTATCTTCTAAACCTGGTAAAATTTCACGGCAGGATCCTGTTACATAC
ATTTCAGAAACAGATGAAGAAGATGACTTTATGTGTAAGAAGGCGGCCTCTAAATCAAAA
GAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAACATGAAAAAGAATGAAGAA
AACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACATCAGTACTT
GATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGA
AAAGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAA
TTACAGCTTGATGAAGATGCGGAGCTGCAGAGGCAGTTGCATGAAGATGAAGAGTTTGCC
CAGACATTAGCCATGTTGGATGAAGACCCAAGACCAAAAAGCTCGAAAGACACAGAAGCG
A
>OriGene 3' read for NM_002913 unedited
CGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTAATGTGGAACATTTTTATTCCTAAT
TTATACTTTNGAAAGATATCTGACAATGTACAAAGAATACCCAGTTCTAGGTGGTGACTG
TTTTGTATAATCATTTATTTACAAAATTTCACCACATAATAGGAACTGCCTTTAAAAAAA
ATCAGCATTGGTCCTATAAAGGCCATTAGGATAAATTCTTAAGCCTACAAAAGACGCCTT
ATTACTACTGCTCCCAGGCTCATTATTGTTAACTAAAATCTGACTCCAAAGCCCAGGTGT
AGTTTCTAGTTCCAATTCTACAGTCATTCAGAAGCACGTCTAACTAGATTGACAGAGGAC
AGTGGAGGACCCAAGCAGTCCTATCAACCTCTATAAGAGGTGTACATAAGCCTACTGCTC
ATACCCTTCTAGCCATACCACCCTTTATTTATAATGGGACACCAGGTCATCCCATTATGC
TAAAACATGGTTGCTCTGGGAAAAAACAAGGCTTTCTCTAGACCAGCTGGACTGGTCAGG
AGGGAGAGTAAAAAGTGGCTGTCGCTAGTGAAAAATGGTTTCATTTCTTCGAACTTTTCC
TNTTCCTTTCTGGGCTCCTTATCTTTTTTCTGGTTTGAAGGCTTTGAAGATTTTGTCTTT
TTTCTTGATCATGGCATCCAGTTTCTATAGCATCCTAGCTCTTTTCTCATTCAGATTAGA
AAGTCATCCTAATTTAATTTCTTCATTGTATTCCCGAATCCAAGGGATGGGCTTGTGCCT
GGCTCTAAAAGCCTTTATACCTTGAAGTGAGTTTGAAGTAAGGTCGGCCTTCTTTATTGA
AGCCTCTGAGAAAGCTGTTTTACCTTGGAACCATCTTGAAAAGAACTATGTTGCCGCCCA
CCTGTGATTTCCAGAATTCCAAGTCTTCTTTATAAATAATGGGCCCTTATGCAAAACATC
TGATCTCCGTTCTCTTTGGGAGCAAGCTTGGAAGGGCCTCCTT
Restriction Sites NotI-NotI     
ACCN NM_002913
Insert Size 4170 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002913.3, NP_002904.3
RefSeq Size 4896 bp
RefSeq ORF 4896 bp
Locus ID 5981
Cytogenetics 4p14
Domains BRCT, AAA, AAA
Protein Families Transcription Factors
Protein Pathways DNA replication, Mismatch repair, Nucleotide excision repair
Gene Summary 'This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]'
Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.

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