RFC1 (NM_002913) Human Untagged Clone

CAT#: SC118344

RFC1 (untagged)-Human replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC1
• Synonyms: A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002913, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGA
  ATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAATAG
  CTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCAGAT
  TCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCTTCTA
  AACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGACTTTAT
  GTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAAC
  ATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACAT
  CAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGAAA
  AGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTACAGCTTGAT
  GAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCCATGTTGGATG
  AAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCTGTCCAAGCCAA
  TTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCAGATGAAAGAAAG
  AGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACATTCCAAGTCATCAG
  CTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATGAAAAGAAAAGAAGA
  GAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATTAAATTGAAAGGAGAG
  ACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGTCCTGAAGATTCTGAAA
  AGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAGGCTCTGGGCTCCAA
  AGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATCACAGGCGTGCTGGAGTCT
  ATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTAACAGGAAATGTCAGCAAGA
  AAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGAC
  AAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCAGGCAAGAAATCCAAGTATGAA
  ATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACACCCCAAAAAAATGTCCAAGGAA
  AAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGCAGGCCGACTTCCAAAAGGGACAG
  TTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGCCTGGATTTCAAGGAGCAGGTGGCT
  GAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGACAGCAGTGAAAACAAAGTGGAAAATT
  TGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATAATTGGACAGCAAGGTGACCAGAGCTG
  TGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGTTCTTCCGAAGATAAAAAACACGCAAAG
  TTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTTAAAGCAGCGTTGCTGTCAGGCCCTCCTGGTG
  TTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAGTTGGGATACAGCTACGTGGAACTGAATGCAAG
  TGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTTGCTGAGTCACTGAACAATACCAGCATCAAAGGC
  TTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACGAAACATGCTCTCATCATGGATGAAGTAGATGGCA
  TGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAATTAATTGGCCTGATAAAACATACTAAAATTCCCAT
  TATTTGTATGTGCAATGATAGAAATCATCCCAAGATTCGCTCTCTGGTTCATTATTGTTTTGATCTTCGT
  TTTCAAAGACCTCGGGTTGAACAGATTAAGGGTGCTATGATGTCTATTGCATTTAAAGAAGGTTTAAAGA
  TTCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCCAATCAAGATATCAGACAGGTTTTACATAATCT
  GAGTATGTGGTGTGCACGAAGTAAAGCATTAACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCCAAA
  AAGGATATCAAAATGGGCCCATTTGATGTTGCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTCACA
  TGTCACTTGTGGACAAGTCAGATCTCTTTTTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGAAAA
  TTACATACACGTGAAGCCTGTAGCAGCAGGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGAGCA
  GCAGACAGCATATGCGATGGTGACCTAGTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTCTGC
  CTGCGCAGGCCATTTATGCCAGTGTTCTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCCCAC
  CTTCCCAAGCTGGCTGGGGAAGCACTCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCCTTG
  CATATGAGTCTCAGAACTTACTCCAGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGGATG
  CACTTGTACAGCCCTTGACCTCACAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACACATA
  TTATTTGATGAAAGAAGACTTTGAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCCTTT
  TCAAAGCTGGATCCCAAGGTGAAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTCCAT
  ACTCACTTCAAGCTATAAAGGCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGAAGA
  ATTAAATGAAGATGACTCTCAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATCAAG
  AAAAAGACAAAATCTTCAAAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAAAAA
  GTTCGAAGAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002913 unedited
  GGGGTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTGCACT
  TCGCGGGAGAAGTTGTTGGCGCGAATGGATCCTGAGCCTCGATAACAGATTCCTCAACCG
  GCCCACCCGCCAGCCAGCCAGCGCCTTCATCCTGGGGCTGCGATGGACATTCGGAAATTC
  TTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGAATGAGAAA
  ACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAAT
  AGCTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATC
  TATGATTCAGATTCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCA
  GAAAAACTGCCAGTATCTTCTAAACCTGGTAAAATTTCACGGCAGGATCCTGTTACATAC
  ATTTCAGAAACAGATGAAGAAGATGACTTTATGTGTAAGAAGGCGGCCTCTAAATCAAAA
  GAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAACATGAAAAAGAATGAAGAA
  AACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACATCAGTACTT
  GATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGA
  AAAGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAA
  TTACAGCTTGATGAAGATGCGGAGCTGCAGAGGCAGTTGCATGAAGATGAAGAGTTTGCC
  CAGACATTAGCCATGTTGGATGAAGACCCAAGACCAAAAAGCTCGAAAGACACAGAAGCG
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002913 unedited
  CGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTAATGTGGAACATTTTTATTCCTAAT
  TTATACTTTNGAAAGATATCTGACAATGTACAAAGAATACCCAGTTCTAGGTGGTGACTG
  TTTTGTATAATCATTTATTTACAAAATTTCACCACATAATAGGAACTGCCTTTAAAAAAA
  ATCAGCATTGGTCCTATAAAGGCCATTAGGATAAATTCTTAAGCCTACAAAAGACGCCTT
  ATTACTACTGCTCCCAGGCTCATTATTGTTAACTAAAATCTGACTCCAAAGCCCAGGTGT
  AGTTTCTAGTTCCAATTCTACAGTCATTCAGAAGCACGTCTAACTAGATTGACAGAGGAC
  AGTGGAGGACCCAAGCAGTCCTATCAACCTCTATAAGAGGTGTACATAAGCCTACTGCTC
  ATACCCTTCTAGCCATACCACCCTTTATTTATAATGGGACACCAGGTCATCCCATTATGC
  TAAAACATGGTTGCTCTGGGAAAAAACAAGGCTTTCTCTAGACCAGCTGGACTGGTCAGG
  AGGGAGAGTAAAAAGTGGCTGTCGCTAGTGAAAAATGGTTTCATTTCTTCGAACTTTTCC
  TNTTCCTTTCTGGGCTCCTTATCTTTTTTCTGGTTTGAAGGCTTTGAAGATTTTGTCTTT
  TTTCTTGATCATGGCATCCAGTTTCTATAGCATCCTAGCTCTTTTCTCATTCAGATTAGA
  AAGTCATCCTAATTTAATTTCTTCATTGTATTCCCGAATCCAAGGGATGGGCTTGTGCCT
  GGCTCTAAAAGCCTTTATACCTTGAAGTGAGTTTGAAGTAAGGTCGGCCTTCTTTATTGA
  AGCCTCTGAGAAAGCTGTTTTACCTTGGAACCATCTTGAAAAGAACTATGTTGCCGCCCA
  CCTGTGATTTCCAGAATTCCAAGTCTTCTTTATAAATAATGGGCCCTTATGCAAAACATC
  TGATCTCCGTTCTCTTTGGGAGCAAGCTTGGAAGGGCCTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002913
• Insert Size: 4170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002913.3, NP_002904.3
• RefSeq Size: 4896 bp
• RefSeq ORF: 4896 bp
• Locus ID: 5981
• Cytogenetics: 4p14
• Domains: BRCT, AAA, AAA
• Protein Families: Transcription Factors
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.