PTPN12 (NM_002835) Human Untagged Clone

CAT#: SC118362

PTPN12 (untagged)-Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPN12
• Synonyms: PTP-PEST; PTPG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002835 edited
  GCGGCCGCGAATTCGGCACGAGGGCCGGGGGGACGCGGGAGGATGGAGCAAGTGGAGATC
  CTGAGGAAATTCATCCAGAGGGTCCAGGCCATGAAGAGTCCTGACCACAATGGGGAGGAC
  AACTTCGCCCGGGACTTCATGCGGTTAAGAAGATTGTCTACCAAATATAGAACAGAAAAG
  ATATATCCCACAGCCACTGGAGAAAAAGAAGAAAATGTTAAAAAGAACAGATACAAGGAC
  ATACTGCCATTTGATCACAGCCGAGTTAAATTGACATTAAAGACTCCTTCACAAGATTCA
  GACTATATCAATGCAAATTTTATAAAGGGCGTCTATGGGCCAAAAGCATATGTAGCAACT
  CAAGGACCTTTAGCAAATACAGTAATAGATTTTTGGAGGATGATATGGGAGTATAATGTT
  GTGATCATTGTAATGGCCTGCCGAGAATTTGAGATGGGAAGGAAAAAATGTGAGCGCTAT
  TGGCCTTTGTATGGAGAAGACCCCATAACGTTTGCACCATTTAAAATTTCTTGTGAGGAT
  GAACAAGCAAGAACAGACTACTTCATCAGGACACTCTTACTTGAATTTCAAAATGAATCT
  CGTAGGCTGTATCAGTTTCATTATGTGAACTGGCCAGACCATGATGTTCCTTCATCATTT
  GATTCTATTCTGGACATGATAAGCTTAATGAGGAAATATCAAGAACATGAAGATGTTCCT
  ATTTGTATTCATTGCAGTGCAGGCTGTGGAAGAACAGGTGCCATTTGTGCCATAGATTAT
  ACGTGGAATTTACTAAAAGCTGGGAAAATACCAGAGGAATTTAATGTATTTAATTTAATA
  CAAGAAATGAGAACACAAAGGCATTCTGCAGTACAAACAAAGGAGCAATATGAACTTGTT
  CATAGAGCTATTGCCCAACTGTTTGAAAAACAGCTACAACTATATGAAATTCATGGAGCT
  CAGAAAATTGCTGATGGAGTGAATGAAATTAACACTGAAAACATGGTCAGCTCCATAGAG
  CCTGAAAAACAAGATTCTCCTCCTCCAAAACCACCAAGGACCCGCAGTTGCCTTGTTGAA
  GGGGATGCTAAAGAAGAAATACTGCAGCCACCGGAACCTCATCCAGTGCCACCCATCTTG
  ACACCTTCTCCCCCTTCAGCTTTTCCAACAGTCACTACTGTGTGGCAGGACAATGATAGA
  TACCATCCAAAGCCAGTGTTGCATATGGTTTCATCAGAACAACATTCAGCAGACCTCAAC
  AGAAACTATAGTAAATCAACAGAACTTCCAGGGAAAAATGAATCAACAATTGAACAGATA
  GATAAAAAATTGGAACGAAATTTAAGTTTTGAGATTAAGAAGGTCCCTCTCCAAGAGGGA
  CCAAAAAGTTTTGATGGGAACACACTTTTGAATAGGGGACATGCAATTAAAATTAAATCT
  GCTTCACCTTGTATAGCTGATAAAATCTCTAAGCCACAGGAATTAAGTTCAGATCTAAAT
  GTCGGTGATACTTCCCAGAATTCTTGTGTGGACTGCAGTGTAACACAATCAAACAAAGTT
  TCAGTTACTCCACCAGAAGAATCCCAGAATTCAGACACACCTCCAAGGCCAGACCGCTTG
  CCTCTTGATGAGAAAGGACATGTAACGTGGTCATTTCATGGACCTGAAAATGCCATACCC
  ATACCTGATTTATCTGAAGGCAATTCCTCAGATATCAACTATCAAACTAGGAAAACTGTG
  AGTTTAACACCAAGTCCTACAACACAAGTTGAAACACCTGATCTTGTGGATCATGATAAC
  ACTTCACCACTCTTCAGAACACCCCTCAGTTTTACTAATCCACTTCACTCTGATGACTCA
  GACTCAGATGAAAGAAACTCTGATGGTGCTGTGACCCAGAATAAAACTAATATTTCAACA
  GCAAGTGCCACAGTTTCTGCTGCCACTAGTACTGAAAGCATTTCTACTAGGAAAGTATTG
  CCAATGTCCATTGCTAGACATAATATAGCAGGAACAACACATTCAGGTGCTGAAAAAGAT
  GTTGATGTTAGTGAAGATTCACCTCCTCCCCTACCTGAAAGAACTCCTGAATCGTTTGTG
  TTAGCAAGTGAACATAATACACCTGTAAGATCGGAATGGAGTGAACTTCAAAGTCAGGAA
  CGATCTGAACAAAAAAAGTCTGAAGGCTTGATAACCTCTGAAAATGAGAAATGTGATCAT
  CCAGCGGGAGGTATTCACTATGAAATGTGCATAGAATGTCCACCTACTTTCAGTGACAAG
  AGAGAACAAATATCAGAAAATCCAACAGAAGCCACAGATATTGGTTTTGGTAATCGATGT
  GGAAAACCCAAAGGACCAAGAGATCCACCTTCAGAATGGACATGATTCAAGGAGCTAGAA
  GACACTTTAAGTTATACTGGAAAATTCAGGTGCCACTGAAAGCCAGATTTATAGTATTCC
  ATCTTTAATATGTGGGACTAACAGCAGTGTAGATTGTTACCTTAATATTTTTTGCTGGGA
  CCATCTACCTGCCTTATACTACACTTAGGAAAAAGTATTACATATGGTTTATTTTGAAAC
  TTCAAGTATTATTGCCTTAATGTCTCTTAACCCTGTTACACGCTGCTTGTAGACATGTTA
  ATATAGTAATACCTTTATGATATATTGAGTTTAAAGACTACTCTTTTTCTGTTTTATCAT
  GTATGCATTATTTTGTATATGTACAGGGCAAGTAGGTATATAATTTGATAAAGTTGCAAT
  TGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002835 unedited
  GTTCGCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCCGGGGGGA
  CGCGGGAGGATGGAGCAAGTGGAGATCCTGAGGAAATTCATCCAGAGGGTCCAGGCCATG
  AAGAGTCCTGACCACAATGGGGAGGACAACTTCGCCCGGGACTTCATGCGGTTAAGAAGA
  TTGTCTACCAAATATAGAACAGAAAAGATATATCCCACAGCCACTGGAGAAAAAGAAGAA
  AATGTTAAAAAGAACAGATACAAGGACATACTGCCATTTGATCACAGCCGAGTTAAATTG
  ACATTAAAGACTCCTTCACAAGATTCAGACTATATCAATGCAAATTTTATAAAGGGCGTC
  TATGGGCCAAAAGCATATGTAGCAACTCAAGGACCTTTAGCAAATACAGTAATAGATTTT
  TGGAGGATGATATGGGAGTATAATGTTGTGATCATTGTAATGGCCTGCCGAGAATTTGAG
  ATGGGAAGGAAAAAATGTGAGCGCTATTGGCCTTTGTATGGAGAAGACCCCATAACGTTT
  GCACCATTTAAAATTTCTTGTGAGGATGAACAAGCAAGAACAGACTACTTCATCAGGACA
  CTCTTACTTGAATTTCAAAATGAATCTCGTAGGCTGTATCAGTTTCATTATGTGAACTGG
  CCAGACCATGATGTTCCTTCATCATTTGATTCTATTCTGGACATGATAAGCTTAATGAGG
  AAATATCAAGAACATGAAGATGTTCCTATNTGTATTCATTGCAGTGCAGGCTGTGGAAGA
  ACAGGTGCCATTTGTGCCATAGATTATACGTGGAAATTACTANAAGCTGGGAAAATACCA
  GAGGAATTTAATGTATTTAANTTAATACCAGAAATGAGAACACAAGGCATCCTGCAGTAC
  CAACCAAGGAGCATATGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002835 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCACTGTAAAACTGTTT
  GTTTTTTTTAATGACAGGTCAAATATCCTGAACACATACATGTATTTTGATAGTCTTCTA
  ATCCCACTTGAGTTCAAATATTAAAGAAACACACACAAGCTAAGAGAATTCAATATTGTT
  CCAAGGCACTTACTGAATCTCTGACTAGATGAAAACAGGAACAATTAGGATGAACAATTA
  GACTGTATATAATATAAAAATAACTTTTTGCTTACAATTTACAAAATTATTATTACAATC
  TTTGATCTCTGATAACCTGCAAAGCTGGCTGCATGAATCCAGCAAGTTTAAAATTCAGAA
  ACTATTTTCTAAAACTCCAGGGCAAATAATTTACAAATAAAGTACACAAAGATTTAGACC
  ATGCAGAAATTTCTTACATCTTCTGTTAATAATATTTCAATTGCAACTTTATCAAATTAT
  ATACCTACTTGCCCTGTACATATACAAAATAATGCATACATGATAAAACAGAAAAAGAGT
  AGTCCTTAAACTCAATATATCATAAAGGTATTACTATATTAACATGTCTACAAGCAGCGT
  GTAACAGGGTTAAGAGACATTAAGGCAATAATACTTGAAGTTTCAAAATAAACCATATGT
  AATACTTTTTCCTAAGTGTAGTATAAGGCAGGTAGATGGTCCCAGCAAAAAATATTAAGG
  TAACAATCTACACTGCTGTTAGTCCCACATATTAAAGATGGAATACTATAAATCTGGCTT
  TCAGTGGCACCTGAATTTCCAGTATAACTTAAAGGGTCTTCTAGCTCCCTGATCATGTCC
  ATTCTGAAAGTGGATCTCTGGGTCCTTGGGGTTTTCACATCGATTACAAAACCAATATCT
  GTGCTTCTGTTGGGATTTCTGAATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002835
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002835.2, NP_002826.2
• RefSeq Size: 3161 bp
• RefSeq ORF: 2343 bp
• Locus ID: 5782
• Cytogenetics: 7q11.23
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).