Nectin 2 (NECTIN2) (NM_002856) Human Untagged Clone

CAT#: SC118376

PVRL2 (untagged)-Human poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NECTIN2
• Synonyms: CD112; HVEB; PRR2; PVRL2; PVRR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002856 edited
  GAATTCGGCACGAGGGCCGGGGGTGCCGAGCCGGGCGGGGAGAGCTGGGCCGGGAGAGCA
  GAACAGGGAGGCTAGAGCGCAGCGGGAACCGGCCCGGAGCCGGAGCCGGAGCCCCACAGG
  CACCTACTAAACCGCCCAGCCGATCGGCCCCCACAGAGTGGCCCGCGGGCCTCCGGCCGG
  GCCCAGTCCCCTCCCGGGCCCTCCATGGCCCGGGCCGCTGCCCTCCTGCCGTCGAGATCG
  CCGCCGACGCCGCTGCTGTGGCCGCTGCTGCTGCTGCTGCTCCTGGAAACCGGAGCCCAG
  GATGTGCGAGTTCAAGTGCTACCCGAGGTGCGAGGCCAGCTCGGGGGCACCGTGGAGCTG
  CCGTGCCACCTGCTGCCACCTGTTCCTGGACTGTACATCTCCCTGGTGACCTGGCAGCGC
  CCAGATGCACCTGCGAACCACCAGAATGTGGCCGCCTTCCACCCTAAGATGGGTCCCAGC
  TTCCCCAGCCCGAAGCCTGGCAGCGAGCGGCTGTCCTTCGTCTCTGCCAAGCAGAGCACT
  GGGCAAGACACAGAGGCAGAGCTCCAGGACGCCACGCTGGCCCTCCACGGGCTCACGGTG
  GAGGACGAGGGCAACTACACTTGCGAGTTTGCCACCTTCCCCAAGGGGTCCGTCCGAGGG
  ATGACCTGGCTCAGAGTCATAGCCAAGCCCAAGAACCAAGCTGAGGCCCAGAAGGTCACG
  TTCAGCCAGGACCCTACGACAGTGGCCCTCTGCATCTCCAAAGAGGGCCGCCCACCTGCC
  CGGATCTCCTGGCTCTCATCCCTGGACTGGGAAGCCAAAGAGACTCAGGTGTCAGGGACC
  CTGGCCGGAACTGTCACTGTCACCAGCCGCTTCACCTTGGTGCCCTCGGGCCGAGCAGAT
  GGTGTCACGGTCACCTGCAAAGTGGAGCATGAGAGCTTCGAGGAACCAGCCCTGATACCT
  GTGACCCTCTCTGTACGCTACCCTCCTGAAGTGTCCATCTCCGGCTATGATGACAACTGG
  TACCTCGGCCGTACTGATGCCACCCTGAGCTGTGACGTCCGCAGCAACCCAGAGCCCACG
  GGCTATGACTGGAGCACGACCTCAGGCACCTTCCCGACCTCCGCAGTGGCCCAGGGCTCC
  CAGCTGGTCATCCACGCAGTGGACAGTCTGTTCAATACCACCTTCGTCTGCACAGTCACC
  AATGCCGTGGGCATGGGCCGCGCTGAGCAGGTCATCTTTGTCCGAGAAACCCCCAGGGCC
  TCGCCCCGAGATGTGGGCCCGCTGGTGTGGGGGGCCGTGGGGGGGACACTGCTGGTGCTG
  CTGCTTCTGGCTGGGGGGTCCTTGGCCTTCATCCTGCTGAGGGTGAGGAGGAGGAGGAAG
  AGCCCTGGAGGAGCAGGAGGAGGAGCCAGTGGCGACGGGGGATTCTACGATCCGAAAGCT
  CAGGTGTTGGGAAATGGGGACCCCGTCTTCTGGACACCAGTAGTCCCTGGTCCCATGGAA
  CCAGATGGCAAGGATGAGGAGGAGGAGGAGGAGGAAGAGAAGGCAGAGAAAGGCCTCATG
  TTGCCTCCACCCCCAGCACTCGAGGATGACATGGAGTCCCAGCTGGACGGCTCCCTCATC
  TCACGGCGGGCAGTTTATGTGTGACCTGGACACAGACAGAGACAGAGCCAGGCCCGGCCC
  TCCCGCCCCCGACCTGACCACGCCGGCCTAGGGTTCCAGACTGGTTGGACTTGTTCGTCT
  GGACGACACTGGAGTGGAACACTGCCTCCCACTTTCTTGGGACTTGGAGGGAGGTGGAAC
  AGCACACTGGACTTCTCCCGTCTCTAGGGCTGCATGGGGAGCCCGGGGAGCTGAGTAGTG
  GGGATCCAGAGAGGACCCCCGCCCCCAGAGACTTGGTTTTGGCTCCAGCCTTCCCCTGGC
  CCCGTGACACTCAGGAGTTAATAAATGCCTTGGAGGAAAAAAAAAAAAAAAAAAAAAAAA
  CTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002856 unedited
  TTCACCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCCGGGGGTG
  CCGAGCCGGGCGGGNGAGACTGGGCCGGGAGAGCAGAACAGGAGGCTAGAGCGCAGCGGG
  AACCGGCCCGGAGCCGGAGCCGGAGCCCCACAGGCACCTACTAAACCGCCCAGCCGATCG
  GCCCCCACAGAGTGGCCCGCGGGCCTCCGGCCGGGCCCAGTCCCCTCCCGGGCCCTCCAT
  GGCCCGGGCCGCTGCCCTCCTGCCGTCGAGATCGCCGCCGACGCCGCTGCTGTGGCCGCT
  GCTGCTGCTGCTGCTCCTGGAAACCGGAGCCCAGGATGTGCGAGTTCAAGTGCTACCCGA
  GGTGCGAGGCCAGCTCGGGGGCACCGTGGAGCTGCCGTGCCACCTGCTGCCACCTGTTCC
  TGGACTGTACATCTCCCTGGTGACCTGGCAGCGCCCAGATGCACCTGCGAACCACCAGAA
  TGTGGCCGCCTTCCACCCTAAGATGGGTCCCAGCTTCCCCAGCCCGAAGCCTGGCAGCGA
  GCGGCTGTCCTTCGTCTCTGCCAAGCAGAGCACTGGGCAAGACACAGAGGCAGAGCTCCA
  GGACGCCACGCTGGCCCTCCACGGGCTCACGGTGGAGGACGAGGGCAACTACACTTGCGA
  GTTTGCCACCTTCCCCAAGGGTCCGTCCGAGGGATGACCTGNCTCAGAGTCATAGCCAAG
  CCCAGAACCAAGCTGAGGCCCAGAAAGTCACGTTCAGCCAGGACCCTACGACAGTGGCCC
  TCTGCATCTCCAAGAAGGCCGGCCACCTGCCCGAACTCCTGCCTCTCATCCCTGGACTGG
  GAACCAAAGAGACTCCAGTGTCAGGGACCCTGGCCCGAACTGTACTGTCACCAGCCGCTT
  CACCTTGTGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002856 unedited
  CGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTCCTCCAGGCATT
  TATTAACTCCTGAGTGTCACGGGGCCAGGGGAAGGCTGGAGCCAAAACCAAGTCTCTGGG
  GGCGGGGGTCCTCTCTGGATCCCCACTACTCAGCTCCCCGGGCTCCCCATGCAGCCCTAG
  AGACGGGAGAAGTCCAGGGGGCTGTTCCACCTACCTCCAAGTCCCAAGAAAGGGGGAGGC
  AGTGCCCCACTCCAGGGCCGCTCAGACGAACAAGTCCAACCAGTCTGGACCCCTAGGCCG
  ACGTGGACAGGCCGGGGGCGGAAGGAACGGTCACTGCTCTGAATCTGACTGCGCCCACAC
  CACACATTAACCGAACCCTTGAGACATGAGGGAATCAAACCCCTGGCAACCACTGCCACA
  ACACAACGCTGGGGGAGGCGGCTACATCGGCTATTTTTTTGCTCACTCTCTCTCCCCCGA
  CCTCCCCTATCTTGCTCTTTGCTCACCGGAATCTCCGCCCTCTGCCCCCTTTATTAGGGT
  TCTCTTTTCACTCTCTCACCCCTTCCCCATCCTCAACTCCCTCTCTCCCTGCCCCCCCTC
  CCCGTACCTCCGGCTGTTTCTCATTCCCCCCCTTTCCCCTGTCAGGGCACTCTCCGCTTT
  CCGCCCGGCAATTATTCTCTTCTTCCCCTCTTCGTCGTCGATCTTTATTTTCTTCTTTAA
  TCCTTCAGTCCCTCTGGACTTCTCCCTCACACGCTTGCACTTTTACATATGTATCCTCCA
  CAATGTCCCTCCCTCCTCAACGCCCCACCCTCAATCTCCCTCATCCTGCGTACCTATCCC
  CCCCCCGCCCTCGCCTGTTGCTTTCTCGTCCTACCAGTCTCCATTACTTTTCCACTCTTA
  CCCTGACTCTCATTTCCCCCCACATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002856
• Insert Size: 1910 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002856.1, NP_002847.1
• RefSeq Size: 1928 bp
• RefSeq ORF: 1440 bp
• Locus ID: 5819
• Cytogenetics: 19q13.32
• Domains: ig, IGv, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (alpha) has multiple differences in the coding region, compared to variant delta, one of which results in an early stop codon. The resulting protein (isoform alpha) has a distinct C-terminus and is shorter than isoform delta.