P5CS (ALDH18A1) (NM_002860) Human Untagged Clone

CAT#: SC118378

ALDH18A1 (untagged)-Human aldehyde dehydrogenase 18 family, member A1 (ALDH18A1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH18A1
• Synonyms: ADCL3; ARCL3A; GSAS; P5CS; PYCS; SPG9; SPG9A; SPG9B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118378 sequence for NM_002860 edited (data generated by NextGen Sequencing)
  ATGTTGAGTCAAGTTTACCGCTGTGGGTTCCAGCCCTTCAACCAACATCTTCTGCCCTGG
  GTCAAGTGTACAACCGTCTTCAGATCTCATTGTATCCAGCCTTCAGTCATCAGACATGTT
  CGTTCTTGGAGCAACATCCCGTTTATCACTGTACCCCTCAGTCGTACACATGGCAAGTCC
  TTCGCCCACCGCAGTGAGCTGAAGCATGCCAAGAGAATCGTGGTGAAGCTCGGCAGTGCC
  GTGGTGACCCGAGGGGATGAATGTGGCCTGGCCCTGGGGCGCTTGGCATCTATTGTTGAG
  CAGGTATCAGTGCTGCAGAATCAGGGCAGAGAGATGATGCTGGTGACCAGTGGAGCCGTA
  GCCTTTGGCAAACAACGCTTGCGCCATGAGATCCTTCTGTCTCAGAGCGTGCGGCAGGCC
  CTCCACTCGGGGCAGAACCAGCTGAAAGAAATGGCAATTCCAGTCTTAGAGGCACGAGCC
  TGTGCAGCTGCCGGACAGAGTGGGCTGATGGCCTTGTATGAGGCTATGTTTACCCAGTAC
  AGCATCTGTGCTGCCCAGATTTTGGTGACCAATTTGGATTTCCATGATGAGCAGAAGCGC
  CGGAACCTCAATGGAACACTTCATGAACTCCTTAGAATGAACATTGTCCCCATTGTCAAC
  ACAAATGATGCTGTTGTCCCCCCAGCTGAGCCCAACAGTGACCTGCAGGGGGTAAATGTT
  ATTAGTGTTAAAGATAATGATAGCCTGGCTGCCCGACTGGCTGTGGAAATGAAAACTGAT
  CTCTTGATTGTTCTTTCAGATGTAGAAGGCCTTTTTGACAGCCCCCCAGGTTCAGATGAT
  GCAAAGCTTATTGATATATTTTATCCCGGAGATCAGCAGTCTGTGACATTTGGAACCAAG
  TCTAGAGTGGGAATGGGTGGCATGGAAGCCAAGGTGAAAGCAGCCCTCTGGGCTTTGCAA
  GGTGGCACTTCTGTTGTTATTGCCAATGGAACCCACCCAAAGGTGTCTGGGCACGTCATC
  ACAGACATTGTGGAGGGGAAGAAAGTTGGTACCTTCTTTTCAGAAGTAAAGCCTGCAGGC
  CCTACTGTTGAGCAGCAGGGAGAAATGGCGCGATCTGGAGGAAGGATGTTGGCCACCTTG
  GAACCTGAGCAGAGAGCAGAAATTATCCATCATCTGGCTGATCTGTTGACGGACCAGCGT
  GATGAGATCCTGTTAGCCAACAAAAAAGACTTGGAGGAGGCAGAGGGGAGACTTGCAGCT
  CCTCTGCTGAAACGTTTAAGCCTCTCCACATCCAAATTGAACAGCCTGGCCATCGGTCTG
  CGACAGATCGCAGCCTCCTCCCAGGACAGCGTGGGACGTGTTTTGCGCCGCACCCGAATC
  GCCAAAAACTTGGAACTGGAACAAGTGACTGTCCCAATTGGAGTTCTGCTGGTGATCTTT
  GAATCTCGTCCTGACTGTCTACCCCAGGTGGCAGCTTTGGCTATCGCAAGTGGCAATGGC
  TTGTTACTCAAAGGAGGGAAGGAGGCTGCACACAGCAACCGGATTCTCCACCTCCTGACC
  CAGGAGGCTCTCTCAATCCATGGAGTCAAGGAGGCCGTGCAACTGGTGAATACCAGAGAA
  GAAGTTGAAGATCTTTGCCGCCTAGACAAAATGATAGATCTGATCATTCCACGTGGCTCT
  TCCCAGCTGGTCAGAGACATCCAGAAAGCTGCTAAGGGGATTCCAGTGATGGGGCACAGC
  GAAGGGATCTGTCACATGTATGTGGATTCCGAGGCCAGTGTTGATAAGGTCACCAGGCTA
  GTCAGAGACTCTAAATGTGAATATCCAGCTGCCTGTAATGCTTTGGAGACTTTGTTAATC
  CACCGGGATCTGCTCAGGACACCATTATTTGACCAGATCATTGATATGCTGAGAGTGGAA
  CAGGTAAAAATTCATGCAGGCCCCAAATTTGCCTCCTATCTGACCTTCAGCCCCTCCGAA
  GTGAAGTCACTCCGAACTGAGTATGGGGACCTGGAATTATGCATTGAAGTAGTGGACAAC
  GTTCAGGATGCCATTGACCACATCCACAAGTATGGCAGCTCCCACACGGATGTCATCGTC
  ACAGAGGACGAAAACACAGCGGAGTTCTTCCTGCAGCACGTAGACAGTGCCTGTGTGTTC
  TGGAATGCCAGCACTCGCTTTTCTGATGGTTACCGCTTTGGACTGGGAGCTGAAGTGGGA
  ATCAGTACATCGAGAATCCACGCCCGGGGACCAGTAGGACTTGAGGGACTGCTTACTACT
  AAGTGGCTGCTGCGAGGGAAGGACCACGTGGTCTCAGATTTCTCAGAGCATGGAAGTTTA
  AAATATCTTCATGAGAACCTCCCTATTCCTCAGAGAAACACCAACTGA
  
  Clone variation with respect to NM_002860.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002860 unedited
  TTCACCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGTGGACGGAA
  GAAAAAGNAAGTGAGCGAGCGCCGGAATCAGCCCGGCGTGGAGTGCGGACCCGCGGTAGT
  GCCAGGGGGCGAAGGCGGCGGTGGTGAGGAAGATACTTTGGTTAGTGACCACATCGCAGC
  ATGTTGAGTCAAGTTTACCGCTGTGGGTTCCAGCCCTTCAACCAACATCTTCTGCCCTGG
  GTCAAGTGTACAACCGTCTTCAGATCTCATTGTATCCAGCCTTCAGTCATCAGACATGTT
  CGTTCTTGGAGCAACATCCCGTTTATCACTGTACCCCTCAGTCGTACACATGGCAAGTCC
  TTCGCCCACCGCAGTGAGCTGAAGCATGCCAAGAGAATCGTGGTGAAGCTCGGCAGTGCC
  GTGGTGACCCGAGGGGATGAATGTGGCCTGGCCCTGGGGCGCTTGGCATCTATTGTTGAG
  CAGGTATCAGTGCTGCAGAATCAGGGCAGAGAGATGATGCTGGTGACCAGTGGAGCCGTA
  GCCTTTGGCAAACAACGCTTGCGCCATGAGATCCTTCTGTCTCAGAGCGTGCGGCAGGCC
  CTCCACTCGGGGCAGAACCAGCTGAAAGAAATGGCAATTCCAGTCTTAGAGGCACGAGCC
  TGTGCAGCTGCCGGACAGAGTGGGCTGATGGCCTTGTATGAGGCTATGTTTACCCAGTAC
  AGCATCTGTGCTGCCCAGATTTTGGTGACCAATTTGGATTTCCATGATGAGCAGAAGCGC
  CGGAACCTCAATGGAACACTTCATGAACTNCTTAGAATGAACATTGTCCCCATTGTCAAC
  ACANATGATGCTGTTGTCCNCCCAGCTGAGCCCACAGTGACCTGCAGGNGGTAAATGNTA
  TTAGTGNTAAAGATATGATAACCTGNCTGCCCGACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002860 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATAAAAACTTTATTTCTCAAAA
  AGGGGGAAGAATCCAAAGTATTAAAATAATCCTCTAATTTTTGTTTTGGAGGAAAAGGGT
  AGGACGACAAAATAATTCATACAAAAATTTCAAACATCACTGCTGTAGATATTCCTCCAG
  CTCAGTGGGGAACATCCTGAAACTTGCATCTCCTGCTGCAGCTTGGGTTCCAGCTGCATC
  ACGGGGAACACAGCATCTCCTGGATGCAGGAAGCTGCAAGCATCTGGAATGCTTATTAAT
  TTACCCCACAAATAAATACAAAAGGTCAATCTTCCCAGTGGAAATGATTCCATCGATTTT
  TGTGACTTTCTGATGAGAATGCTAAAAAGAAGAGTTGCCCCTTTTCTAAAATTCCAAATC
  TTTCCTTTTTGAAGATGACTGCATGTGAAAGAAATAAAATGTGAAAAGATTTGTTAAGGA
  TGACTGGCTCTAGTACCAACTAAATGCCAAGGGGGACTGTAAGTCACTGAGGTGACACAA
  AGCAGCCATGGGTTTTCCTCGCCTTTTTTATGGGGAAAATGCACCTTTCAATCCTAGAAG
  ATAATTGGACTTGGCAAAGTCCCTATCGGTAGCAACTATTTTCTTACTTTAAAAAAAAAG
  GGTGAGCTGGGAGCCAGACTGTGCACATCAGCCAAGATTGCTATTGCCAAACGGAGCCCA
  GAAGCATCCAGGTACACTTTCCACAGGCAGACCCTACCAGGAACTGGNGAGACAGAGCGG
  GCTCTCTCCTGAGATAGACAAGTTAACGTGAAGACCTTNTGGAAAATCCCNGGTTTCCCT
  GCTCTTTCAGNTGTGTTTTCTGAGGATAGGAGGTCTATGAGATATTTAACTNCTGCTTGA
  GAATTGAACCACTGTCCTTCCTCCAGAGCACTATATAGCAGTCTCAGTCTCTGTCCCCGC
  GGNTNCTCAGTCTGATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002860
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002860.3, NP_002851.2
• RefSeq Size: 3470 bp
• RefSeq ORF: 2388 bp
• Locus ID: 5832
• Cytogenetics: 10q24.1
• Domains: aakinase, aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1), also known as P5CSL, encodes the longest isoform (1). Variants 1, 4, and 5 all encode the same isoform (1).