PRPS2 (NM_002765) Human Untagged Clone

CAT#: SC118398

PRPS2 (untagged)-Human phosphoribosyl pyrophosphate synthetase 2 (PRPS2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRPS2
• Synonyms: PRSII
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118398 sequence for NM_002765 edited (data generated by NextGen Sequencing)
  ATGCCCAACATCGTGCTGTTCAGCGGCAGCTCGCATCAGGACCTGTCCCAGCGCGTGGCC
  GACCGCCTGGGCCTGGAGCTGGGCAAGGTGGTCACGAAGAAGTTCAGCAACCAGGAGACC
  AGCGTGGAGATTGGTGAAAGCGTGAGAGGGGAAGATGTCTACATCATCCAGAGCGGCTGC
  GGGGAAATTAACGACAACCTGATGGAACTCCTCATCATGATCAATGCCTGCAAGATTGCG
  TCATCATCCAGAGTAACTGCCGTGATCCCGTGTTTCCCATACGCCCGACAAGATAAAAAG
  GACAAGAGTCGTGCCCCAATTTCTGCAAAACTTGTGGCCAATATGCTGTCGGTGGCTGGG
  GCGGATCACATCATCACCATGGACCTGCATGCTTCTCAGATACAGGGATTCTTTGATATT
  CCTGTGGATAATTTGTATGCGGAGCCCGCAGTCCTGCAGTGGATTCGGGAAAACATTGCC
  GAGTGGAAGAACTGTATCATTGTTTCACCTGACGCAGGGGGAGCCAAAAGGGTTACATCA
  ATTGCAGACAGGTTGAATGTGGAATTTGCTTTGATCCACAAAGAGAGGAAGAAGGCGAAT
  GAAGTGGACCGGATGGTCCTGGTGGGCGACGTGAAGGACCGTGTGGCCATCCTCGTGGAT
  GACATGGCTGACACTTGCGGCACCATCTGCCATGCTGCGGACAAGCTGCTGTCAGCTGGA
  GCCACCAAAGTGTATGCTATCCTTACCCATGGGATCTTCTCTGGACCAGCTATTTCCAGA
  ATAAATAATGCCGCCTTTGAGGCTGTTGTCGTCACAAACACAATTCCGCAAGAGGACAAA
  ATGAAACACTGCACCAAGATTCAGGTCATTGACATTTCCATGATCTTGGCCGAAGCAATC
  CGAAGGACACACAATGGGGAATCCGTGTCCTACCTGTTCAGCCATGTCCCGCTATAA
  
  Clone variation with respect to NM_002765.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002765 unedited
  NNGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGTCGCTG
  TCGCTGTTGCCTCCGCCACCTCCTCCGCCGCCGCGCGCCCCTCGGAGTTCCGCGCCCCAC
  CATGCCCAACATCGTGCTGTTCAGCGGCAGCTCGCATCAGGACCTGTCCCAGCGCGTGGC
  CGACCGCCTGGGCCTGGAGCTGGGCAAGGTGGTCACGAAGAAGTTCAGCAACCAGGAGAC
  CAGCGTGGAGATTGGTGAAAGCGTGAGAGGGGAAGATGTCTACATCATCCAGAGCGGCTG
  CGGGGAAATTAACGACAACCTGATGGAACTCCTCATCATGATCAATGCCTGCAAGATTGC
  GTCATCATCCAGAGTAACTGCCGTGATCCCGTGTTTCCCATACGCCCGACAAGATAAAAA
  GGACAAGAGTCGTGCCCCAATTTCTGCAAAACTTGTGGCCAATATGCTGTCGGTGGCTGG
  GGCGGATCACATCATCACCATGGACCTGCATGCTTCTCAGATACAGGGATTCTTTGATAT
  TCCTGTGGATAATTTGTATGCGGAGCCCGCAGTCCTGCAGTGGATTCGGGAAAACATTGC
  CGAGTGGAAGAACTGTATCATTGTTTCACCTGACGCAGGGGGAGCCAAAAGGGTTACATC
  AATTGCAGACAGGTTGAATGGTGGGATTTGCTTTGATCCACAAAGAGAGGGAAGAAGGCG
  AATGGAAGTGGCCCGCGGATGGTCCCTGGTGGGNCGACCGTGAAAGAACCCGTTGTGGCC
  ATCCCTCGTTGGGATGACCATGGGCTGGACACTTTGCGGGCACCATTCTGCCATGNCTGC
  GGNACAAGGCTGCTGTCCAAGCTGGAGCCACCCAAGAGTGTATGGCTATCCCTACCCCCT
  GGGGGATCTTTCTCTGGGACCAGCTAATTTCCAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002765 unedited
  CGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTGAACCCCCCCAAAAAGATTTTATTGAAAGGTTTTAA
  TACCGGGGTAAAGGATTTTAAAATTTTACTTTTTAAACCAAAACAAGCCCTGGAAAGGAA
  ATACCCCCGAATTTAGTGGGGGGAGTATCTAAAAAAAAAAGGGGTCTTTTGAGTTTTTTT
  AAGTGGACTAAGGGAATTTAAGGGGGGGGGTAAACAAAAAACTTTGGGAAATTCAAATTT
  CTCTGGCCCCCGGTTTTGGCCAAAAAAATGCCAGGGCCCCCCCCCTTTAATAAAATTGGA
  CCGGACCCAGGGTTTTGGGGGGCTCACTTAAGTTTCCCCCCCCGGAGCCCCAAAAAAGGG
  CCAAATTTTGGGATTAAACTGGTCCCAAAAAAGTTTTTATTACCTTTGGGAACGGGAAAC
  CCCAAAAAGGCAAGGGCTGAATTTCCATTGGGGGGGGGTAAAACCAACCCCATTGGGTCA
  TAAATGGTTTTTGAAGGGTTTTAAAAAACAAAGGGGGGGCTAAAAAATGAAAAAAACCCT
  CAGTTTTTAATTCAAAAAAAGACAAGGCAGGGTCCTATATTCTTTTTAAAAATCTGGAAA
  CATTATTTTAAAACAAGCCCTATACCTTCTTAGGCTTTAAATTTAAACTTAACCGAAAGG
  AATCCCTTTAAACCCGGGGGCTTTAGGCCCTGGGGAGTGCCCAAAAAATTTTGGAATTTT
  AGGAGCCCCCTTTCCGGAATGGAAGTTTGTTTAAAAAGGGCTTTAAAAAGGACCCAAAAC
  CCTGTCTGAATTGGTAAGGTTCCCCCCAAAGGGTGCGGGGCCATATTACCTAAAAATTGG
  CCAATAACCCCCACCCGAGGGTTTTGAACCGGTTTTCAAAAAAACAAAAGGGAATCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002765
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002765.2, NP_002756.1
• RefSeq Size: 2517 bp
• RefSeq ORF: 957 bp
• Locus ID: 5634
• Cytogenetics: Xp22.2
• Domains: Pribosyltran
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway, Purine metabolism
• Gene Summary: 'This gene encodes a phosphoribosyl pyrophosphate synthetase that plays a central role in the synthesis of purines and pyrimidines. The encoded protein catalyzes the synthesis of 5-phosphoribosyl 1-pyrophosphate from ATP and D-ribose 5-phosphate. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.