PSMD1 (NM_002807) Human Untagged Clone

CAT#: SC118428

PSMD1 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1


  "NM_002807" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PSMD1
Synonyms P112; Rpn2; S1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118428 sequence for NM_002807 edited (data generated by NextGen Sequencing)
ATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACCACAGCTTAAG
GAATTTGCACTACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGAAATTTCCGAG
TCCGTAGACAAAATAGAGGTTTTATACGAAGATGAAGGTTTCCGGAGTCGGCAGTTTGCA
GCCTTAGTGGCATCTAAAGTATTTTATCACCTGGGGGCTTTTGAGGAGTCTCTGAATTAT
GCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACTCTGAATATGTGGAAACTATT
ATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGATTTGCCTGAA
GGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAATGTTCCAGCGA
TGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGACACGAAGACTG
GACGTCTTTGAAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTTAGCTTATAGC
CTTAAGCTCTGCATGTCTTTAATGCAGAATAAACAGTTTCGGAATAAAGTACTAAGAGTT
CTAGTTAAAATCTACATGAACTTGGAGAAACCTGATTTCATCAATGTTTGTCAGTGCTTA
ATTTTCTTAGATGATCCTCAGGCTGTGAGTGATATCTTAGAGAAACTGGTAAAGGAAGAC
AACCTCCTGATGGCATATCAGATTTGTTTTGATTTGTATGAAAGTGCTAGCCAGCAGTTT
TTGTCATCTGTAATCCAGAATCTTCGAACTGTTGGCACCCCTATTGCTTCTGTGCCTGGA
TCCACTAATACGGGTACTGTTCCGGGATCAGAGAAAGACAGTGACTCGATGGAAACAGAA
GAAAAGACAAGCAGTGCATTTGTAGGAAAGACACCAGAAGCCAGTCCAGAGCCTAAGGAC
CAGACTTTGAAAATGATTAAAATTTTAAGTGGTGAAATGGCTATTGAGTTACATCTGCAG
TTCTTAATACGAAACAATAATACAGACCTCATGATTCTAAAAAACACAAAGGATGCAGTA
CGGAATTCTGTATGTCATACTGCAACCGTTATAGCAAACTCTTTTATGCACTGTGGGACA
ACCAGTGACCAGTTTCTTAGAGATAATTTGGAATGGTTAGCCAGAGCCACTAACTGGGCA
AAATTTACTGCTACAGCCAGTTTGGGTGTAATTCATAAGGGTCATGAAAAAGAAGCATTA
CAGTTAATGGCAACATACCTTCCCAAGGATACTTCTTCAGGATCAGCCTATCAGGAAGGT
GGAGGTCTCTATGCACTAGGTCTTATTCATGCCAATCATGGTGGTGATATAATTGACTAT
CTGCTTAATCAGCTTAAGAACGCCAGCAATGATATCGTTAGACACGGTGGCAGTCTGGGC
CTTGGTTTGGCAGCCATGGGAACTGCACGTCAAGATGTTTATGATTTGCTAAAAACAAAC
CTTTATCAGGATGATGCAGTAACAGGGGAAGCAGCTGGCCTGGCCCTAGGTTTGGTTATG
TTGGGCTCTAAAAATGCTCAGGCTATTGAGGACATGGTTGGTTATGCACAAGAAACTCAA
CATGAGAAGATTCTGCGTGGTCTTGCAGTTGGCATAGCTTTAGTAATGTATGGGAGGATG
GAAGAGGCTGATGCTCTCATTGAATCTCTCTGTCGTGACAAGGACCCAATTCTTCGAAGG
TCTGGAATGTATACTGTAGCCATGGCTTATTGTGGCTCTGGTAACAACAAAGCAATTCGA
CGCCTGCTACATGTTGCTGTAAGTGATGTTAATGATGATGTCAGGAGGGCAGCAGTAGAA
TCACTTGGGTTCATTCTATTCAGAACCCCTGAACAGTGCCCAAGTGTTGTCTCTTTGTTG
TCAGAGAGTTACAACCCTCATGTGCGCTACGGAGCTGCAATGGCCTTGGGGATATGCTGT
GCTGGTACAGGAAACAAGGAAGCCATTAATTTGCTAGAACCAATGACAAACGACCCCGTG
AACTACGTGAGGCAAGGGGCACTCATAGCTTCAGCTCTCATCATGATCCAGCAGACTGAA
ATCACTTGTCCAAAGGTGAATCAGTTCAGACAGCTGTATTCCAAAGTCATCAATGATAAG
TATGATGATGTCATGGCCAAGTTTGGCGCTATTCTGGCCCAGGGCATACTGGATGCAGGT
GGTCATAATGTCACAATCTCCTTGCAGTCCAGGACTGGGCATACTCATATGCCTTCTGTG
GTTGGCGTCCTTGTATTTACCCAGTTTTGGTTCTGGTTTCCTCTTTCACACTTCCTGTCA
TTGGCTTATACCCCTACCTGTGTCATTGGCCTTAACAAGGACTTAAAGATGCCGAAAGTT
CAGTATAAATCGAACTGTAAACCATCCACATTTGCATATCCTGCCCCTCTGGAAGTACCA
AAAGAAAAAGAAAAGGAAAAGGTTTCTACTGCTGTATTATCTATAACTGCCAAGGCTAAA
AAGAAGGAAAAAGAAAAGGAAAAAAAGGAGGAGGAGAAAATGGAAGTGGATGAGGCAGAG
AAAAAGGAGGAAAAAGAGAAGAAAAAAGAACCTGAGCCAAACTTCCAGTTATTGGATAAC
CCAGCCCGAGTTATGCCTGCCCAGCTTAAGGTCCTAACCATGCCGGAGACCTGTAGATAC
CAGCCTTTCAAACCACTCTCTATTGGAGGCATCATCATTCTGAAGGATACCAGTGAAGAC
ATTGAGGAGCTGGTGGAACCTGTGGCAGCACATGGCCCAAAAATCGAGGAGGAGGAACAA
GAGCCAGAACCCCCAGAACCATTTGAGTATATTGATGATTAA

Clone variation with respect to NM_002807.3
1297 c=>t;2161 c=>t
>OriGene 5' read for NM_002807 unedited
GGGTTCAAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCCTG
AGGAGGCGACTGACTGAGCAGCGCACCCGGGGAGCAAGGAGGCGCGGTGAACTGAGCGGC
CCCTGAGCTGACAGATACACTGCGCAGCTGGAACGGCGAGCGAGCCGACGGGCGAGTGAG
GGGCGCAGCCATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACC
ACAGCTTAAGGAATTTGCACTACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGA
AATTTCCGAGTCCGTAGACAAAATAGAGGTTTTATACGAAGATGAAGGTTTCCGGAGTCG
GCAGTTTGCAGCCTTAGTGGCATCTAAAGTATTTTATCACCTGGGGGCTTTTGAGGAGTC
TCTGAATTATGCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACTCTGAATATGT
GGAAACTATTATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGA
TTTGCCTGAAGGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAAT
GTTCCAGCGATGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGAC
ACGAAGACTGGACGTCTTTGAAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTT
AGCTTATAGCCTTAAGCTCTGCATGTCTTTAATGCAGAATANACAGTTTCGGAATAAAGT
ACTAAGAGTNCTAGTTAAAATCTACATGAACTTGGAGAAACCTGATTTCATCAATGTTTG
TCAGTGCTTAAATTTCTTAGATGATCCCTCAGCTGTGAGTGATATCTTAGAGAAACTGGT
NAAGGAGAN
>OriGene 3' read for NM_002807 unedited
AATCTCTGNCCGCGGCCGCATTCTANAGTCGGTTTTTTTTTTTTTTTTTTGTCCATCTGG
AGATTTTATATTTATTTGAAAACCGGGGGGGGGGAAAGAGCACACTCAACAGTCATATAT
CTTCTCTTCAACCAAAGACTTGGAGACAGGGTTCTTCAGGTCAGTGCAGAGGCAACGATA
CATGCATTGAATAGCAGGTCTCGGCATGAATTTCCTCATATTCATTCCCAATATGAGCCT
GGACAGTGGGCTTCAGATAAGCATGTGAGATCCTCTGGCCCTTAATCATCAATATACTCA
AATGGTTCTGGGGGTTCTGGCTCTTGTTCCTCCTCCTCGATTTTTGGGCCATGTGCTGCC
ACAGGTTCCACCAGCTCCTCAATGTCTTCACTGGTATCCTTCAGAATGATGATGCCTCCA
ATACAGAGTGGTTTGAAAGGCTGGTATCTACAGGTCTCCGGCATGGTTAGGACCTTAAGC
TGGGCAGGCATAACTCGGGCTGGGTTATTCAATAACTGGAAGTTTGGCTCAGGCTCTTTC
TTCTTCTCTTTTTCCTCCTTTTTCTCTGCCTCATTCACTTCCATTTTCTCCTCCTCCTTT
TTTTCCTTTTTTTTTTCCTTCTTTCTTACCTTGGCCGTTATAGATAATACAGCAGTAGAA
ACCTTTTCCCTTTTTTTTTCTTCTGGTCCTTCCACACGGGCCAGATATGCAAAAGTGGCT
GGTTTACACGTTCATCCATATCGACCTTTCCGCACTCTTTAAGCACTTTGCAAGGGCCAC
TGACCAGGTAGGGGTATAAGCCCCACGCCCGCAATGCCGAAAGAAGAAACCCGACCCCTA
ACTGGGTTACAACCAAGGCGCCCACCCCCAAAAGGCATATGCAATTGCCCCATNTCTGGA
ATGCCCCGGAATTGTGACATTACGCACACCCGCATCCATTC
Restriction Sites NotI-NotI     
ACCN NM_002807
Insert Size 3200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002807.2, NP_002798.2
RefSeq Size 3227 bp
RefSeq ORF 2862 bp
Locus ID 5707
Cytogenetics 2q37.1
Domains PC_rep
Protein Pathways Proteasome
Gene Summary 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. There is evidence that this proteasome and its subunits interact with viral proteins, including those of coronaviruses. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Aug 2020]'
Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1).

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