PSMD1 (NM_002807) Human Untagged Clone

CAT#: SC118428

PSMD1 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD1
• Synonyms: P112; Rpn2; S1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118428 sequence for NM_002807 edited (data generated by NextGen Sequencing)
  ATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACCACAGCTTAAG
  GAATTTGCACTACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGAAATTTCCGAG
  TCCGTAGACAAAATAGAGGTTTTATACGAAGATGAAGGTTTCCGGAGTCGGCAGTTTGCA
  GCCTTAGTGGCATCTAAAGTATTTTATCACCTGGGGGCTTTTGAGGAGTCTCTGAATTAT
  GCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACTCTGAATATGTGGAAACTATT
  ATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGATTTGCCTGAA
  GGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAATGTTCCAGCGA
  TGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGACACGAAGACTG
  GACGTCTTTGAAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTTAGCTTATAGC
  CTTAAGCTCTGCATGTCTTTAATGCAGAATAAACAGTTTCGGAATAAAGTACTAAGAGTT
  CTAGTTAAAATCTACATGAACTTGGAGAAACCTGATTTCATCAATGTTTGTCAGTGCTTA
  ATTTTCTTAGATGATCCTCAGGCTGTGAGTGATATCTTAGAGAAACTGGTAAAGGAAGAC
  AACCTCCTGATGGCATATCAGATTTGTTTTGATTTGTATGAAAGTGCTAGCCAGCAGTTT
  TTGTCATCTGTAATCCAGAATCTTCGAACTGTTGGCACCCCTATTGCTTCTGTGCCTGGA
  TCCACTAATACGGGTACTGTTCCGGGATCAGAGAAAGACAGTGACTCGATGGAAACAGAA
  GAAAAGACAAGCAGTGCATTTGTAGGAAAGACACCAGAAGCCAGTCCAGAGCCTAAGGAC
  CAGACTTTGAAAATGATTAAAATTTTAAGTGGTGAAATGGCTATTGAGTTACATCTGCAG
  TTCTTAATACGAAACAATAATACAGACCTCATGATTCTAAAAAACACAAAGGATGCAGTA
  CGGAATTCTGTATGTCATACTGCAACCGTTATAGCAAACTCTTTTATGCACTGTGGGACA
  ACCAGTGACCAGTTTCTTAGAGATAATTTGGAATGGTTAGCCAGAGCCACTAACTGGGCA
  AAATTTACTGCTACAGCCAGTTTGGGTGTAATTCATAAGGGTCATGAAAAAGAAGCATTA
  CAGTTAATGGCAACATACCTTCCCAAGGATACTTCTTCAGGATCAGCCTATCAGGAAGGT
  GGAGGTCTCTATGCACTAGGTCTTATTCATGCCAATCATGGTGGTGATATAATTGACTAT
  CTGCTTAATCAGCTTAAGAACGCCAGCAATGATATCGTTAGACACGGTGGCAGTCTGGGC
  CTTGGTTTGGCAGCCATGGGAACTGCACGTCAAGATGTTTATGATTTGCTAAAAACAAAC
  CTTTATCAGGATGATGCAGTAACAGGGGAAGCAGCTGGCCTGGCCCTAGGTTTGGTTATG
  TTGGGCTCTAAAAATGCTCAGGCTATTGAGGACATGGTTGGTTATGCACAAGAAACTCAA
  CATGAGAAGATTCTGCGTGGTCTTGCAGTTGGCATAGCTTTAGTAATGTATGGGAGGATG
  GAAGAGGCTGATGCTCTCATTGAATCTCTCTGTCGTGACAAGGACCCAATTCTTCGAAGG
  TCTGGAATGTATACTGTAGCCATGGCTTATTGTGGCTCTGGTAACAACAAAGCAATTCGA
  CGCCTGCTACATGTTGCTGTAAGTGATGTTAATGATGATGTCAGGAGGGCAGCAGTAGAA
  TCACTTGGGTTCATTCTATTCAGAACCCCTGAACAGTGCCCAAGTGTTGTCTCTTTGTTG
  TCAGAGAGTTACAACCCTCATGTGCGCTACGGAGCTGCAATGGCCTTGGGGATATGCTGT
  GCTGGTACAGGAAACAAGGAAGCCATTAATTTGCTAGAACCAATGACAAACGACCCCGTG
  AACTACGTGAGGCAAGGGGCACTCATAGCTTCAGCTCTCATCATGATCCAGCAGACTGAA
  ATCACTTGTCCAAAGGTGAATCAGTTCAGACAGCTGTATTCCAAAGTCATCAATGATAAG
  TATGATGATGTCATGGCCAAGTTTGGCGCTATTCTGGCCCAGGGCATACTGGATGCAGGT
  GGTCATAATGTCACAATCTCCTTGCAGTCCAGGACTGGGCATACTCATATGCCTTCTGTG
  GTTGGCGTCCTTGTATTTACCCAGTTTTGGTTCTGGTTTCCTCTTTCACACTTCCTGTCA
  TTGGCTTATACCCCTACCTGTGTCATTGGCCTTAACAAGGACTTAAAGATGCCGAAAGTT
  CAGTATAAATCGAACTGTAAACCATCCACATTTGCATATCCTGCCCCTCTGGAAGTACCA
  AAAGAAAAAGAAAAGGAAAAGGTTTCTACTGCTGTATTATCTATAACTGCCAAGGCTAAA
  AAGAAGGAAAAAGAAAAGGAAAAAAAGGAGGAGGAGAAAATGGAAGTGGATGAGGCAGAG
  AAAAAGGAGGAAAAAGAGAAGAAAAAAGAACCTGAGCCAAACTTCCAGTTATTGGATAAC
  CCAGCCCGAGTTATGCCTGCCCAGCTTAAGGTCCTAACCATGCCGGAGACCTGTAGATAC
  CAGCCTTTCAAACCACTCTCTATTGGAGGCATCATCATTCTGAAGGATACCAGTGAAGAC
  ATTGAGGAGCTGGTGGAACCTGTGGCAGCACATGGCCCAAAAATCGAGGAGGAGGAACAA
  GAGCCAGAACCCCCAGAACCATTTGAGTATATTGATGATTAA
  
  Clone variation with respect to NM_002807.3
  1297 c=>t;2161 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002807 unedited
  GGGTTCAAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCCTG
  AGGAGGCGACTGACTGAGCAGCGCACCCGGGGAGCAAGGAGGCGCGGTGAACTGAGCGGC
  CCCTGAGCTGACAGATACACTGCGCAGCTGGAACGGCGAGCGAGCCGACGGGCGAGTGAG
  GGGCGCAGCCATGATCACCTCGGCCGCTGGAATTATTTCTCTTCTGGATGAAGATGAACC
  ACAGCTTAAGGAATTTGCACTACACAAATTGAATGCAGTTGTTAATGACTTCTGGGCAGA
  AATTTCCGAGTCCGTAGACAAAATAGAGGTTTTATACGAAGATGAAGGTTTCCGGAGTCG
  GCAGTTTGCAGCCTTAGTGGCATCTAAAGTATTTTATCACCTGGGGGCTTTTGAGGAGTC
  TCTGAATTATGCTCTTGGAGCAGGGGACCTCTTCAATGTCAATGATAACTCTGAATATGT
  GGAAACTATTATAGCAAAATGCATTGATCACTACACCAAACAATGTGTGGAAAATGCAGA
  TTTGCCTGAAGGAGAAAAAAAACCAATTGACCAGAGATTGGAAGGCATCGTAAATAAAAT
  GTTCCAGCGATGTCTAGATGATCACAAGTATAAACAGGCTATTGGCATTGCTCTGGAGAC
  ACGAAGACTGGACGTCTTTGAAAAGACCATACTGGAGTCGAATGATGTCCCAGGAATGTT
  AGCTTATAGCCTTAAGCTCTGCATGTCTTTAATGCAGAATANACAGTTTCGGAATAAAGT
  ACTAAGAGTNCTAGTTAAAATCTACATGAACTTGGAGAAACCTGATTTCATCAATGTTTG
  TCAGTGCTTAAATTTCTTAGATGATCCCTCAGCTGTGAGTGATATCTTAGAGAAACTGGT
  NAAGGAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002807 unedited
  AATCTCTGNCCGCGGCCGCATTCTANAGTCGGTTTTTTTTTTTTTTTTTTGTCCATCTGG
  AGATTTTATATTTATTTGAAAACCGGGGGGGGGGAAAGAGCACACTCAACAGTCATATAT
  CTTCTCTTCAACCAAAGACTTGGAGACAGGGTTCTTCAGGTCAGTGCAGAGGCAACGATA
  CATGCATTGAATAGCAGGTCTCGGCATGAATTTCCTCATATTCATTCCCAATATGAGCCT
  GGACAGTGGGCTTCAGATAAGCATGTGAGATCCTCTGGCCCTTAATCATCAATATACTCA
  AATGGTTCTGGGGGTTCTGGCTCTTGTTCCTCCTCCTCGATTTTTGGGCCATGTGCTGCC
  ACAGGTTCCACCAGCTCCTCAATGTCTTCACTGGTATCCTTCAGAATGATGATGCCTCCA
  ATACAGAGTGGTTTGAAAGGCTGGTATCTACAGGTCTCCGGCATGGTTAGGACCTTAAGC
  TGGGCAGGCATAACTCGGGCTGGGTTATTCAATAACTGGAAGTTTGGCTCAGGCTCTTTC
  TTCTTCTCTTTTTCCTCCTTTTTCTCTGCCTCATTCACTTCCATTTTCTCCTCCTCCTTT
  TTTTCCTTTTTTTTTTCCTTCTTTCTTACCTTGGCCGTTATAGATAATACAGCAGTAGAA
  ACCTTTTCCCTTTTTTTTTCTTCTGGTCCTTCCACACGGGCCAGATATGCAAAAGTGGCT
  GGTTTACACGTTCATCCATATCGACCTTTCCGCACTCTTTAAGCACTTTGCAAGGGCCAC
  TGACCAGGTAGGGGTATAAGCCCCACGCCCGCAATGCCGAAAGAAGAAACCCGACCCCTA
  ACTGGGTTACAACCAAGGCGCCCACCCCCAAAAGGCATATGCAATTGCCCCATNTCTGGA
  ATGCCCCGGAATTGTGACATTACGCACACCCGCATCCATTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002807
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002807.2, NP_002798.2
• RefSeq Size: 3227 bp
• RefSeq ORF: 2862 bp
• Locus ID: 5707
• Cytogenetics: 2q37.1
• Domains: PC_rep
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. There is evidence that this proteasome and its subunits interact with viral proteins, including those of coronaviruses. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1).