MEK3 (MAP2K3) (NM_002756) Human Untagged Clone

CAT#: SC118431

MAP2K3 (untagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A


  "NM_002756" in other vectors (4)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MAP2K3
Synonyms MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_002756 edited
GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGGGCCGTCTGCCCGCAGCCAT
GAGCGTGCTCGGCCCCGGTGGAGCCCGCAGTCCTCTAGATTAGTCTCCACCGCCGTCCAG
GACCCACTTGCAGCATGGAGTCGCCCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCA
AAGGAAAATCCAAGAGGAAGAAGGATCTACGGATATCCTGCATGTCCAAGCCACCCGCAC
CCAACCCCACACCCCCCCGGAACCTGGACTCCCGGACCTTCATCACCATTGGAGACAGAA
ACTTTGAGGTGGAGGCTGATGACTTGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATG
GGGTGGTAGAGAAGGTGCGGCACGCCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCC
GGGCCACCGTGAACTCACAGGAGCAGAAGCGGCTGCTCATGGACCTGGACATCAACATGC
GCACGGTCGACTGTTTCTACACTGTCACCTTCTACGGGGCACTATTCAGAGAGGGAGACG
TGTGGATCTGCATGGAGCTCATGGACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGG
ATAAAAACATGACAATTCCAGAGGACATCCTTGGGGAGATTGCTGTGTCTATCGTGCGGG
CCCTGGAGCATCTGCACAGCAAGCTGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATG
TCCTTATCAACAAGGAGGGCCATGTGAAGATGTGTGACTTTGGCATCAGTGGCTACTTGG
TGGACTCTGTGGCCAAGACGATGGATGCCGGCTGCAAGCCCTACATGGCCCCTGAGAGGA
TCAACCCAGAGCTGAACCAGAAGGGCTACAATGTCAAGTCCGACGTCTGGAGCCTGGGCA
TCACCATGATTGAGATGGCCATCCTGCGGTTCCCTTACGAGTCCTGGGGGACCCCGTTCC
AGCAGCTGAAGCAGGTGGTGGAGGAGCCGTCCCCCCAGCTCCCAGCCGACCGTTTCTCCC
CCGAGTTTGTGGACTTCACTGCTCAGTGCCTGAGGAAGAACCCCGCAGAGCGTATGAGCT
ACCTGGAGCTGATGGAGCACCCCTTCTTCACCTTGCACAAAACCAAGAAGACGGACATTG
CTGCCTTCGTGAAGGAGATCCTGGGAGAAGACTCATAGGGGCTGGGCCTCGGACCCCACT
CCGGCCCTCCAGAGCCCCACAGCCCCATCTGCGGGGGCAGTGCTCACCCACACCATAAGC
TACTGCCATCCTGGCCCAGGGCATCTGGGAGGAACCGAGGGGGCTGCTCCCACCTGGCTC
TGTGGCGAGCCATTTGTCCCAAGTGCCAAAGAAGCAGACCATTGGGGCTCCCAGCCAGGC
CCTTGTCGGCCCCACCAGTGCCTCTCCCTGCTGCTCCTAGGACCCGTCTCCAGCTGCTGA
GATCCTGGACTGAGGGGGCCTGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGG
CTGCCAGTGCCTGGGTGGATGGGCCACCGCCTTGCCCAGCCTGGATGCCATCCAAGTTGT
ATATTTTTTTAATCTCTCGACTGAATGGACTTTGCACACTTTGGCCCAGGGTGGCCACAC
CTCTATCCCGGCTTTGGTGCGGGGTACACAAGAGGGGATGAGTTGTGTGAATACCCCAAG
ACTCCCATGAGGGAGATGCCATGAGCCGCCCAAGGCCTTCCCCTGGCACTGGCAAACAGG
GCCTCTGCGGAGCACACTGGCTCACCCAGTCCTGCCCGCCACCGTTATCGGTGTCATTCA
CCTTTCGTGTTTTTTTTAATTTATCCTCTGTTGATTTTTTCTTTTGCTTTATGGGTTTGG
CTTGTTTTTCTTGCATGGTTTGGAGCTGATCGCTTCTCCCCCACCCCCTAGGGTACCAGC
AGGCAGAGCCTTGCCCTCTGCTCAGGCTGGGGTCCAGTGGGAGGGGCCCAAGATCTCTGC
TCAGAGAAGTGCAGGGGGAGCCTTCCAGCTCACTCTCCCTGAGGACTGGCTTGACAGGGG
CTATGGGTTTGCTTTGGTGTTGTTTTTAAAAAAAGAAAATATATTTTTTTGAAAAAACGA
CTGCCCATCCCGGGTCCTTTCCCTGATGGGTTGGGGCAGTTACCTGGTTGCTGTTTTAAT
TAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_002756 unedited
NGGGTTGGTAGTTGTATACGATCACTATAGGCGGCCGCGAATTCGCACGAGCCTCGTGCC
GAATTCGGCACGAGGGGGCCGTCTGCCCGCAGCCATGAGCGTGCTCGGCCCCGGTGGAGC
CCGCAGTCCTCTAGATTAGTCTCCACCGCCGTCCAGGACCCACTTGCAGCATGGAGTCGC
CCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCAAAGGAAAATCCAAGAGGAAGAAGG
ATCTACGGATATCCTGCATGTCCAAGCCACCCGCACCCAACCCCACACCCCCCCGGAACC
TGGACTCCCGGACCTTCATCACCATTGGAGACAGAAACTTTGAGGTGGAGGCTGATGACT
TGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATGGGGTGGTAGAGAAGGTGCGGCACG
CCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCCGGGCCACCGTGAACTCACAGGAGC
AGAAGCGGCTGCTCATGGACCTGGACATCAACATGCGCACGGTCGACTGTTTCTACACTG
TCACCTTCTACGGGGCACTATTCANAGAGGGAGACGTGTGGATCTGCATGGAGCTCATGG
ACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGGATAAAAACATGACAATTCCAGAGG
ACATCCTTGGGGAGATTGCTGCGTCTATCGTGCGGGCCCTGGAGCATCTGCACAGCGAGC
TGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATGTCCTTATCAACAAGGAGGGCCATG
TGAAGAAGTGTGACTTTGGCATCAGTGGCTACCTGGTGGACTTTGTGGCCCAGACAAGGG
TGCCCGCTGGCAACCCTAACTGGGCCCTGAGAGGTACAACCCAGAGCTGACCCGCAGGGC
TTCCATGTN
>OriGene 3' read for NM_002756 unedited
ATTTGGCCGCGGCCGCATTTTANATCGAGTTTTTTTTTTTTTTTTTTAATTAAAACAGCA
ACCAGGTAACTGCCCCAACCCATCAGGGAAAGGACCCGGGATGGGCAGTCGTTTTTTCAA
AAAAATATATTTTCTTTTTTTAAAAACAACACCAAAGCAAACCCATAGCCCCTGTCAAGC
CAGTCCTCAGGGAGAGTGAGCTGGAAGGCTCCCCCTGCACTTCTCTGAGCAGAGATCTTG
GGCCCCTCCCACTGGACCCCAGCCTGAGCAGAGGGCAAGGCTCTGCCTGCTGGTACCCTA
GGGGGTGGGGGAGAAGCGATCAGCTCCAAACCATGCAAGAAAAACAAGCCAAACCCATAA
AGCAAAAGAAAAAATCAACAGAGGATAAATTAAAAAAAACACGAAAGGTGAATGACACCG
ATAACGGTGGCGGGCAGGACTGGGTGAGCCAGTGTGCTCCGCAGAGGCCCTGTTTGCCAG
TGCCAGGGGAAGGCCTTGGGCGGCTCATGGCATCTCCCTCATGGGAGTCTTGGGGTATTC
ACACAACTCATCCCCTCTTGTGTACCCCGCACCAAAGCCGGGATAGAGGTGTGGCCACCC
TGNGCCAAGTGTGCANAGTCCATTCAGTCGAGAGATAAAAAAAATATACACTTGGATGGG
CATCCAGCTGGGCAGGGCGGTGGCCCATCCACCCAGCACTGGCAGCCTGCTGTGCANGGG
CAGCAGCATCCACAGGGGGCATNNCAGCCCNCTCAGTCCAGATCTCAGCAGCTGGAGACG
GGTTCCTAGAGCANCAGGGAGAAGCACTGGTGGGGCCGACAGAGGCCTGGCTGGGAGCCC
ACATGGTCTGCTTCTTTGGCACTTGGNACAAATGCCTCGCCACAGACCAGGTGGGAGCAG
CCCCTCTGTTCCTCCAAATGCCCTGGCCANGATGGCAGTANCTAATGTGTGGGTGAACAC
CTGCCCGCAGATGGGCTGC
Restriction Sites NotI-NotI     
ACCN NM_002756
Insert Size 2210 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002756.2, NP_002747.2
RefSeq Size 2029 bp
RefSeq ORF 957 bp
Locus ID 5606
Cytogenetics 17p11.2
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway
Gene Summary 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (A) represents use of an alternate promoter and 5' UTR and uses a downstream start codon compared to variant B. The resulting isoform (A) is shorter at the N-terminus compared to isoform B. Variants A and D both encode the same isoform (A).

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