MEK3 (MAP2K3) (NM_002756) Human Untagged Clone

CAT#: SC118431

MAP2K3 (untagged)-Human mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP2K3
• Synonyms: MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002756 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGGGCCGTCTGCCCGCAGCCAT
  GAGCGTGCTCGGCCCCGGTGGAGCCCGCAGTCCTCTAGATTAGTCTCCACCGCCGTCCAG
  GACCCACTTGCAGCATGGAGTCGCCCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCA
  AAGGAAAATCCAAGAGGAAGAAGGATCTACGGATATCCTGCATGTCCAAGCCACCCGCAC
  CCAACCCCACACCCCCCCGGAACCTGGACTCCCGGACCTTCATCACCATTGGAGACAGAA
  ACTTTGAGGTGGAGGCTGATGACTTGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATG
  GGGTGGTAGAGAAGGTGCGGCACGCCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCC
  GGGCCACCGTGAACTCACAGGAGCAGAAGCGGCTGCTCATGGACCTGGACATCAACATGC
  GCACGGTCGACTGTTTCTACACTGTCACCTTCTACGGGGCACTATTCAGAGAGGGAGACG
  TGTGGATCTGCATGGAGCTCATGGACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGG
  ATAAAAACATGACAATTCCAGAGGACATCCTTGGGGAGATTGCTGTGTCTATCGTGCGGG
  CCCTGGAGCATCTGCACAGCAAGCTGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATG
  TCCTTATCAACAAGGAGGGCCATGTGAAGATGTGTGACTTTGGCATCAGTGGCTACTTGG
  TGGACTCTGTGGCCAAGACGATGGATGCCGGCTGCAAGCCCTACATGGCCCCTGAGAGGA
  TCAACCCAGAGCTGAACCAGAAGGGCTACAATGTCAAGTCCGACGTCTGGAGCCTGGGCA
  TCACCATGATTGAGATGGCCATCCTGCGGTTCCCTTACGAGTCCTGGGGGACCCCGTTCC
  AGCAGCTGAAGCAGGTGGTGGAGGAGCCGTCCCCCCAGCTCCCAGCCGACCGTTTCTCCC
  CCGAGTTTGTGGACTTCACTGCTCAGTGCCTGAGGAAGAACCCCGCAGAGCGTATGAGCT
  ACCTGGAGCTGATGGAGCACCCCTTCTTCACCTTGCACAAAACCAAGAAGACGGACATTG
  CTGCCTTCGTGAAGGAGATCCTGGGAGAAGACTCATAGGGGCTGGGCCTCGGACCCCACT
  CCGGCCCTCCAGAGCCCCACAGCCCCATCTGCGGGGGCAGTGCTCACCCACACCATAAGC
  TACTGCCATCCTGGCCCAGGGCATCTGGGAGGAACCGAGGGGGCTGCTCCCACCTGGCTC
  TGTGGCGAGCCATTTGTCCCAAGTGCCAAAGAAGCAGACCATTGGGGCTCCCAGCCAGGC
  CCTTGTCGGCCCCACCAGTGCCTCTCCCTGCTGCTCCTAGGACCCGTCTCCAGCTGCTGA
  GATCCTGGACTGAGGGGGCCTGGATGCCCCCTGTGGATGCTGCTGCCCCTGCACAGCAGG
  CTGCCAGTGCCTGGGTGGATGGGCCACCGCCTTGCCCAGCCTGGATGCCATCCAAGTTGT
  ATATTTTTTTAATCTCTCGACTGAATGGACTTTGCACACTTTGGCCCAGGGTGGCCACAC
  CTCTATCCCGGCTTTGGTGCGGGGTACACAAGAGGGGATGAGTTGTGTGAATACCCCAAG
  ACTCCCATGAGGGAGATGCCATGAGCCGCCCAAGGCCTTCCCCTGGCACTGGCAAACAGG
  GCCTCTGCGGAGCACACTGGCTCACCCAGTCCTGCCCGCCACCGTTATCGGTGTCATTCA
  CCTTTCGTGTTTTTTTTAATTTATCCTCTGTTGATTTTTTCTTTTGCTTTATGGGTTTGG
  CTTGTTTTTCTTGCATGGTTTGGAGCTGATCGCTTCTCCCCCACCCCCTAGGGTACCAGC
  AGGCAGAGCCTTGCCCTCTGCTCAGGCTGGGGTCCAGTGGGAGGGGCCCAAGATCTCTGC
  TCAGAGAAGTGCAGGGGGAGCCTTCCAGCTCACTCTCCCTGAGGACTGGCTTGACAGGGG
  CTATGGGTTTGCTTTGGTGTTGTTTTTAAAAAAAGAAAATATATTTTTTTGAAAAAACGA
  CTGCCCATCCCGGGTCCTTTCCCTGATGGGTTGGGGCAGTTACCTGGTTGCTGTTTTAAT
  TAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002756 unedited
  NGGGTTGGTAGTTGTATACGATCACTATAGGCGGCCGCGAATTCGCACGAGCCTCGTGCC
  GAATTCGGCACGAGGGGGCCGTCTGCCCGCAGCCATGAGCGTGCTCGGCCCCGGTGGAGC
  CCGCAGTCCTCTAGATTAGTCTCCACCGCCGTCCAGGACCCACTTGCAGCATGGAGTCGC
  CCGCCTCGAGCCAGCCCGCCAGCATGCCCCAGTCCAAAGGAAAATCCAAGAGGAAGAAGG
  ATCTACGGATATCCTGCATGTCCAAGCCACCCGCACCCAACCCCACACCCCCCCGGAACC
  TGGACTCCCGGACCTTCATCACCATTGGAGACAGAAACTTTGAGGTGGAGGCTGATGACT
  TGGTGACCATCTCAGAACTGGGCCGTGGAGCCTATGGGGTGGTAGAGAAGGTGCGGCACG
  CCCAGAGCGGCACCATCATGGCCGTGAAGCGGATCCGGGCCACCGTGAACTCACAGGAGC
  AGAAGCGGCTGCTCATGGACCTGGACATCAACATGCGCACGGTCGACTGTTTCTACACTG
  TCACCTTCTACGGGGCACTATTCANAGAGGGAGACGTGTGGATCTGCATGGAGCTCATGG
  ACACATCCTTGGACAAGTTCTACCGGAAGGTGCTGGATAAAAACATGACAATTCCAGAGG
  ACATCCTTGGGGAGATTGCTGCGTCTATCGTGCGGGCCCTGGAGCATCTGCACAGCGAGC
  TGTCGGTGATCCACAGAGATGTGAAGCCCTCCAATGTCCTTATCAACAAGGAGGGCCATG
  TGAAGAAGTGTGACTTTGGCATCAGTGGCTACCTGGTGGACTTTGTGGCCCAGACAAGGG
  TGCCCGCTGGCAACCCTAACTGGGCCCTGAGAGGTACAACCCAGAGCTGACCCGCAGGGC
  TTCCATGTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002756 unedited
  ATTTGGCCGCGGCCGCATTTTANATCGAGTTTTTTTTTTTTTTTTTTAATTAAAACAGCA
  ACCAGGTAACTGCCCCAACCCATCAGGGAAAGGACCCGGGATGGGCAGTCGTTTTTTCAA
  AAAAATATATTTTCTTTTTTTAAAAACAACACCAAAGCAAACCCATAGCCCCTGTCAAGC
  CAGTCCTCAGGGAGAGTGAGCTGGAAGGCTCCCCCTGCACTTCTCTGAGCAGAGATCTTG
  GGCCCCTCCCACTGGACCCCAGCCTGAGCAGAGGGCAAGGCTCTGCCTGCTGGTACCCTA
  GGGGGTGGGGGAGAAGCGATCAGCTCCAAACCATGCAAGAAAAACAAGCCAAACCCATAA
  AGCAAAAGAAAAAATCAACAGAGGATAAATTAAAAAAAACACGAAAGGTGAATGACACCG
  ATAACGGTGGCGGGCAGGACTGGGTGAGCCAGTGTGCTCCGCAGAGGCCCTGTTTGCCAG
  TGCCAGGGGAAGGCCTTGGGCGGCTCATGGCATCTCCCTCATGGGAGTCTTGGGGTATTC
  ACACAACTCATCCCCTCTTGTGTACCCCGCACCAAAGCCGGGATAGAGGTGTGGCCACCC
  TGNGCCAAGTGTGCANAGTCCATTCAGTCGAGAGATAAAAAAAATATACACTTGGATGGG
  CATCCAGCTGGGCAGGGCGGTGGCCCATCCACCCAGCACTGGCAGCCTGCTGTGCANGGG
  CAGCAGCATCCACAGGGGGCATNNCAGCCCNCTCAGTCCAGATCTCAGCAGCTGGAGACG
  GGTTCCTAGAGCANCAGGGAGAAGCACTGGTGGGGCCGACAGAGGCCTGGCTGGGAGCCC
  ACATGGTCTGCTTCTTTGGCACTTGGNACAAATGCCTCGCCACAGACCAGGTGGGAGCAG
  CCCCTCTGTTCCTCCAAATGCCCTGGCCANGATGGCAGTANCTAATGTGTGGGTGAACAC
  CTGCCCGCAGATGGGCTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002756
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002756.2, NP_002747.2
• RefSeq Size: 2029 bp
• RefSeq ORF: 957 bp
• Locus ID: 5606
• Cytogenetics: 17p11.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (A) represents use of an alternate promoter and 5' UTR and uses a downstream start codon compared to variant B. The resulting isoform (A) is shorter at the N-terminus compared to isoform B. Variants A and D both encode the same isoform (A).