PPP6C (NM_002721) Human Untagged Clone

CAT#: SC118443

PPP6C (untagged)-Human protein phosphatase 6, catalytic subunit (PPP6C), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP6C
• Synonyms: PP6; PP6C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118443 sequence for NM_002721 edited (data generated by NextGen Sequencing)
  ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCA
  GAGAACGACCTGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAAT
  GTTCAGCCAGTATCAACACCAGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGAC
  CTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCCTGACACAAACTACATATTTATGGGT
  GATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCACTTACCTTCTTGCATTAAAG
  GCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAGACAGATAACA
  CAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
  TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTG
  TGTGTCCATGGTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAA
  CGGAATCAGGAAATTCCTCATAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAA
  GATGTGGATACCTGGGCTATCAGTCCCCGAGGAGCAGGTTGGCTTTTTGGAGCAAAGGTC
  ACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCTGCAGAGCACATCAACTAGTG
  CACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTCTGCTCCTAAT
  TACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
  GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACA
  ACGCCATATTTCCTTTGA
  
  Clone variation with respect to NM_002721.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002721 unedited
  GTTCAGATATAGTATACGACTTCACTATANNGGCGGCCGCGCAATTCGGCACGAGGCCTC
  GTGCCTAATTCGGCACGAGGCTTAAAAGGCGCCGCTAGACCTGGACAAGTATGTGGAAAT
  AGCGCGGCTGTGCAAGTACCTGCCAGAGAACGACCTGAAGCGGCTATGTGACTACGTTTG
  TGACCTCCTCTTAGAAGAGTCAAATGTTCAGCCAGTATTAACACCAGTAACAGTGTGTGG
  AGATATCCATGGACAGTTTTATGACCTTTGTGAACTGTTCAGAACTGGAGGTCAGGTTCC
  TGACACAAACTACATATTTATGGGTGATTTTGTAGACAGAGGTTACTATAGTTTGGAGAC
  CTTCACTTACCTTCTTGCATTAAAGGCTAAATGGCCTGATCGTATTACACTTTTGCGAGG
  AAATCATGAGAGTAGACAGATAACACAGGTCTATGGATTTTATGATGAGTGCCAAACCAA
  ATATGGAAATGCTAATGCCTGGAGATACTGTACCAAAGTTTTTGACATGCTCACAGTAGC
  AGCTTTAATAGATGAGCAGATTTTGTGTGTCCATGGTGGTTTATCTCCTGATATCAAAAC
  ACTGGATCAAATTCGAACCATCGAACGGAATCAGGAAATTCCTCATAAAGGAGCATTTTG
  TGATCTGGTTTGGTCAGATCCTGAAGATGTGGATACCTGNGCTATCAGTCCCCGAGGAGC
  AGGTTGGCTTTTTGGAGCAAAGGTCACAAATGAGTNTGTTCATATCAACAACTTANAACT
  CATCTGCAGAGCACATCAACTAGTGCACGAANGGCTATAATTTATGTTTGATGAGAAGCT
  GGTGACAGTATGGCCTGCTCCTCATTCCTGCTATCCGTGTGGAAAATATGCTCCGATCAT
  GGTCTCCCAAGATGTAAATACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002721 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCTTT
  AAACCAATTTATTTTATATCCATTTAAAATATCATCCAACAAAGGACAATGAAGTTTACA
  TCTGGGACAGGTATACATAACTTGAGGCATTTGGAGTCCATCGTGCCCACTGAATAACAA
  CTTTGCTGCTGAAATTTCTTAAAACTTACCAAACTAAAACAAAAGGCTGAATGATACAAA
  AGGAGGAGTATTTAGGTGTAATCTGTCAATTGGCTTTTAAAAAAAGGAAACACATCCTGT
  TTCCCAGACCAGTAAATAAGCAAGAGCTACATGCAGCAGTGTGAGTATTAAGACATTTCT
  CAAGTCTTCTCAAATGAGTCCAGGGTGGGGATGGGATGGGGGAAAATTGATAGAAAAACT
  TTGCTATAGACACCACAACAAACAATAAATTTAGATAATTTAAAATTTAAAAAAAAAAAG
  GCAAGAGGCAGCATTTCAGCAGCAAAGTGCTCAATAAAAAGTATATTGTAGAGGGTAATA
  CAAGAAAATTGGGGTAAGAAGAGGGCAGAAAAATGGGTCAGCAGGATGGGCGAAGGCCTC
  AAAGGAAATATGGCGTTGTCGTTCTGGGAGGAATAACACGTTCTGAATCTGGAACTGCCC
  GGAATAACTNTGGTTCTCTTGTATTTACATCTTTGAAGACCATGATCGAAGCAATATTTC
  CACAACGATAGCAGTAATTAGGAGCAGACCATACTGTCACCAGCTTCTCATCAACATAAA
  TTTATAGCCTTCGTGCACTAAGTGATGTGCTCTGCAGATGAGTTTTAAGTGTTGAATGAA
  CAACTCATTTGTGACCTTTGCTTCAAAAGCCAACCTGCTCTCTGGGACTGATACCCCAGT
  TTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002721
• Insert Size: 1540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002721.3, NP_002712.1
• RefSeq Size: 1563 bp
• RefSeq ORF: 918 bp
• Locus ID: 5537
• Cytogenetics: 9q33.3
• Domains: Metallophos, PP2Ac
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in the coding region, compared to variant 1; it encodes a shorter isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence and transcripts to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.