Protein Phosphatase 1 beta (PPP1CB) (NM_002709) Human Untagged Clone

CAT#: SC118477

PPP1CB (untagged)-Human protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1CB
• Synonyms: HEL-S-80p; NSLH2; PP-1B; PP1B; PP1beta; PPP1CD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118477 sequence for NM_002709 edited (data generated by NextGen Sequencing)
  ATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGGCTGCTGGAGGTACGAGGA
  TGTCGTCCAGGAAAGATTGTGCAGATGACTGAAGCAGAAGTTCGAGGCTTATGTATCAAG
  TCTCGGGAGATCTTTCTCAGCCAGCCTATTCTTTTGGAATTGGAAGCACCGCTGAAAATT
  TGTGGAGATATTCATGGACAGTATACAGATTTACTGAGATTATTTGAATATGGAGGTTTC
  CCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGACAGAGGAAAGCAGTCTTTG
  GAAACCATTTGTTTGCTATTGGCTTATAAAATCAAATATCCAGAGAACTTCTTTCTCTTA
  AGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAA
  CGAAGATTTAATATTAAATTGTGGAAGACCTTCACTGATTGTTTTAACTGTCTGCCTATA
  GCAGCCATTGTGGATGAGAAGATCTTCTGTTGTCATGGAGGATTGTCACCAGACCTGCAA
  TCTATGGAGCAGATTCGGAGAATTATGAGACCTACTGATGTCCCTGATACAGGTTTGCTC
  TGTGATTTGCTATGGTCTGATCCAGATAAGGATGTGCAAGGCTGGGGAGAAAATGATCGT
  GGTGTTTCCTTTACTTTTGGAGCTGATGTAGTCAGTAAATTTCTGAATCGTCATGATTTA
  GATTTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTTTGCTAAACGA
  CAGTTGGTAACCTTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGA
  ATGATGAGTGTGGATGAAACTTTGATGTGTTCATTTCAGATATTGAAACCATCTGAAAAG
  AAAGCTAAATACCAGTATGGTGGACTGAATTCTGGACGTCCTGTCACTCCACCTCGAACA
  GCTAATCCGCCGAAGAAAAGGTGA
  
  Clone variation with respect to NM_002709.2
  201 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002709 unedited
  AACTCGNGATTTTGTAAACCCGACTTCACTATAGGGCGGCAGCGCAATTCGGCACGAGGG
  CGGCGCGCAAGGGACGTGCGGAGTGAGTGGCGCTGCGGGTGGGGCCGTCGGCGGCGCTGG
  TGAGAGAACGCCGAGCCGTCGCCGCAGCCTCCGCCGCCGAGAAGCCCTTGTTCCCGCTGC
  TGGGAAGGAGAGTCTGTGCCGACAAGATGGCGGACGGGTATTTGAACGTGGACAGCCTCA
  TCACCCGGCTGCTGGAGGTACGAGGATGTCGTCCAGGAAAGATTGTGCAGATGACTGAAG
  CAGAAGTTCGAGGCTTATGTATCAAGTCTCGGGAGATCTTTCTCAGCCAGCCTATTCTTT
  TGGAATTGGAAGCACCGCTGAAAATTTGTGGAGATATTCATGGACAGTATACAGATTTAC
  TGAGATTATTTGAATATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATT
  ATGTGGACAGAGGAAAGCAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTATAAAATCA
  AATATCCAGAGAACTTCTTTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCA
  TTTATGGATTCTATGATGAATGCAAACGAAGATTTAATATTAAATTGTGGAAGACCTTCA
  CTGATTGTTTTAACTGTCTGCCTATAGCAGCCATTGTGGATGAGAAGATCTTCTGTTGTC
  ATGGAGGATTGTCACCAGACCTGGCATCTATGGAGCAGATTCGGAGAATTATGAGACCTA
  CTGATGTCCCTGATACAGGGTTGCTCTGTGATTTGCTATGGTCTGATCCAGATNAGGATG
  TGCAAGGCTGGNNGAGAAATGATCGTGGTGGTTCCTTTACTTTTGGAGCTGATGTAGTCA
  GTAAAATTCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002709 unedited
  CTTGGCCGCGGCCGCAATCTTTAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTCTCCTAA
  GAACTTAGTTTTATTTATTTCAAGGCGTGGTGCCAAGCAAATATTTGTAAAGAACACATT
  TTTATGACTGGAGGCTGTTTAAGTGGTTTTAAGAGTGCTTGAATAATTTTTAAACATGAT
  AAATTTCCATCCTTTAAATTTAATGACCAATATTAATTAGCTTAAGTAGTGATTATTTTT
  TCACATTTAGTCTTTAAAAAAAAAAAAAGGATTCCTATTAACCAACATACTTAGTCTTGA
  CCTGGATGAATTTACAAAAGCCAGACAATGGGATACAGATTAATGTGGTTTAATACTGAG
  ATTAATCAACATTGCCCTTTTTCCCTGCCAAAGGCTTATTTTCTAGACCTGCTTCACTAT
  GACTACTAAAAGTGTACAACTAAAATTTTATTAGAAATCGCTCAGCTTACACACTCCAAT
  CAGGAAGTTATTTAAATCACCTCAGATAAAACCTTTGTGACCTAACCTAATACATCATAT
  GCAGATCATGAATACTTTCAGAAAAGAATCATTTCAATATGTCAAGGACATAGTATCTAT
  GTGAATAAAATGTGTGCCTAAATGTCTCACTGTGAGGAAAAAAAAAATCAGTCAGGCCTT
  CATATCCATTTAGACAACTTTTTACTAANATGGAGAAAACTACCTTTTTAAACTGTGCCC
  TTAAAACCAAAACGGGGATTTAGAAAAAAAAGACCTATGGCATTTTTAAAGAACCCTTTT
  GCCACTGTGCCTGAAAAATGAAATATTCCTTTTGCAGTGAAGGTAATACCAATGTGCGCC
  ATTCCCTAAAAATGGAAGGTTACCACGGTTTCTCTATTTTACACTTTTTCAAAGAACAAT
  TTATAGGGCCAAAAATGCCCACTCTTTGTGGCCCCATTATAGAGGGCCTTATAAGAATGG
  AGGCCCATCCCCTATGAGCCTTAACAATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002709
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002709.2, NP_002700.1
• RefSeq Size: 4991 bp
• RefSeq ORF: 984 bp
• Locus ID: 5500
• Cytogenetics: 2p23.2
• Domains: Metallophos, PP2Ac
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: 'The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. It encodes the longer isoform (1).