PHKA1 (NM_002637) Human Untagged Clone

CAT#: SC118481

PHKA1 (untagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKA1
• Synonyms: PHKA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002637 edited
  TGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAAT
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAGCGGTCGTTGACCTGTTGGC
  GGCGGCCGGGCGGGAGGCTGTGGGCCGCGGCGGTCTCCCGGGGTTGAGGGGAGGATGCAG
  GAGTCACACGCGGAGGCCGGACTGGACTAAGGGGGCAGGAGCGGCGCCTGGGGCGCTGCG
  GCGGGGCTCTAGACCGCGGGAAGCCAGTGCTAGCGCTGGCGCGTGGTGGGCCCCTGAGGC
  CGCCGGAGTCCGGAGAGGGACCGAAGGCTCCGGTGGCACCCGGGCTATTTCTCAGAGGAC
  AATTAGTAACGTGTCGCCATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTAC
  GCTCGACTGGTGCAACAGACCATCCTGTGCCATCAGAATCCAGTGACTGGCTTGCTTCCA
  GCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAATGTGTACAGCATCTTGGCTGTG
  TGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGATAAGGCAAAGGCC
  TATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCAGA
  CAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCA
  AAGTACAACACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAG
  TTGGATGCTACCTCTGTGTACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCAT
  ATCATCCACAGCCTAGATGAAGTCAATTTCATACAGAACCTTGTGTTTTACATTGAAGCT
  GCATATAAAACTGCTGACTTCGGGATATGGGAACGTGGAGACAAGACCAACCAAGGGATC
  TCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGGCAGCCCTGGAAGCATTAGATGAA
  CTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATGAA
  GTACAGCACTGCCAGTCTATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAG
  GTTGATGCTAGTCTACTCTCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAG
  TTGGTGGAGCTCACAAAACAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGT
  CGCTTTCTACGAGATGGATATAAAACTCCTAAAGAGGATCCCAATCGTCTGTACTATGAA
  CCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGTGAATGGCCATTGTTCTGGACATAC
  TTTATTCTTGATGGGGTCTTCAGTGGCAATGCAGAACAGGTTCAAGAATATAAAGAGGCT
  CTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACAGT
  GTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCC
  ATGGGGAAATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCA
  GAGGGATTTTTAGCCCCTGGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCG
  AAGCCCGATGTTGTGGTTCAAGTCTCCATTCTAGCTGAAACAGAAGAAATCAAGACCATT
  TTGAAGGACAAGGGAATTTACGTGGAGACCATTGCTGAGGTATACCCCATCAGAGTACAA
  CCAGCTCGTATTCTCAGCCACATTTATTCCAGCCTAGGATGCAACAATAGAATGAAACTC
  AGTGGACGACCCTACAGACACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTCGG
  AAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTG
  GACAACAAGATGATAGTGGAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGG
  CGGATGACAGGCCAGCCCACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGAT
  GGAACAAGCTTGAATTCAAGTATCCTGGCAGCACTCCGAAAAATGCAAGATGGGTATTTT
  GGTGGGGCAAGGGTTCAAACAGGTAAATTGTCAGAGTTTTTGACAACATCTTGTTGCACA
  CACTTGAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAAGATTATGATGAC
  AACTATGATTACCTGGAATCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATGCT
  CGCTGTGGTGATGAAGTTGCTCGTTATTTAGATCACCTTTTGGCGCACACTGCTCCCCAT
  CCTAAACTAGCCCCTACCTCACAGAAGGGAGGGCTAGATCGGTTCCAAGCTGCTGTGCAA
  ACAACCTGCGACTTAATGTCCTTGGTGACCAAGGCCAAGGAACTGCATGTACAGAATGTT
  CACATGTATCTTCCTACGAAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGAT
  TCACCTCATCCCCGACAGGAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCT
  AGAGACCAGTCTGGGGAGGTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCA
  AGCTTACAGGAACAAGCTGATATCCTCTATATGCTGTATACTATGAAAGGACCTGACTGG
  AACACTGAATTGTATAATGAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTAT
  GGCAAAGTGGGAGAAATTCGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGG
  AAGAAAGTGGAAGCACTTGATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTG
  ACAGTAGGACTTCCTCCAGAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAG
  GCGCTCACTCAGCTGATAGATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACA
  CAGGAAATAATGGTATATCTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAA
  ATGTTTCGACTTCGAATTGGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACTCC
  CTTCGATGCTCAGCTGAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATG
  AAGAATCTCCTGCATCACATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGT
  CCCACTGATTCAAATGTCAGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCA
  ACCAAAACAGAACGAACTGGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCACCTGGA
  ACCTCTATGACTCCAAGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAA
  GATAGTCGTCAAGGTCAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTT
  CCAGTTGGATTTTATCAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTT
  GAAGGGTTTGTCCTTCCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTC
  TCTGTTCATGTGGAGTCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTG
  GTTGAAGCCATCCTTGTCCTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGC
  ATCATTGCTGTGGAAAAAATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGAAA
  ACCCTTGGCGCAGATGATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTCTT
  CTGTATGACAGTGCACCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCC
  GCCACCTACGTGCAGGAGTTCCTGCCCCACAGCATCTGTGCCATGCAATGAGGGCTTTGG
  TTCCTGGCTTCTGGGAGCCTTTTGACAGCTGGTCCCTGCCTCGGTTGATTGTGCATGGAA
  CTAAAATGTTATTGCCTAATCACTCCAACCCTGCCCCTTTCTGTCCCATCCTTCCCAAGA
  AGAGAGAACTTTTTCGATAAACTAACTACTGTAGAAGAAGTGAACACTTACCTGGAGGCT
  CACCTTGCAGAACCAGTGACAATCTTATGAGTATAATGAACACTCAGCCAGGCCTGTCAT
  GATTGGCTTTATTTCTTTCATCATTCATAAAAGTTTGCATGTGTTTTTATTCTCTAGATC
  TGTTACCAATATAGTTTTCTAACTCCTGTTTGGGGAGCAAGTGTTAATAATAACTTATTC
  CTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTG
  AACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002637 unedited
  GTAATTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACCAGGCAGCGGTCGT
  TGACCTGTTGGCGGCGGCCGGGCGGGAGGCTGTGGGCCGCGGCGGTCTCCCGGGGTTGAG
  GGGAGGATGCAGGAGTCACACGCGGAGGCCGGACTGGACTAAGGGGGCAGGAGCGGCGCC
  TGGGGCGCTGCGGCGGGGCTCTAGACCGCGGGAAGCCAGTGCTAGCGCTGGCGCGTGGTG
  GGCCCCTGAGGCCGCCGGAGTCCGGAGAGGGACCGAAGGCTCCGGTGGCACCCGGGCTAT
  TTCTCAGAGGACAATTAGTAACGTGTCGCCATGAGGAGCCGGAGTAACTCCGGGGTCCGG
  CTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTGTGCCATCAGAATCCAGTGACT
  GGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAATGTGTACAGC
  ATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGAT
  AAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCAC
  TGCATGATCAGACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGAT
  AGCCTNCATGCAAAGTACAACACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGG
  GGACACCTGCAGTTGGATGCTACCTCTGTGTACCTGCTCTTCTTAGCCCAAATGACTGCC
  TCAGGACTCCATATCATCCACAGCCTAGATGAAGTCAATTTCATACAGGACCCTTGTGTT
  TTACATTGAAGCTGCATATAAAACTGCTGACTNCGGATATGGGAACGTGGAGACAAGACC
  ACCCAGGGATCTCANAGATGAGCCAGTTCAGTTGGATGGCAAAGCAGCCCTGAAGCATAG
  ATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002637 unedited
  NNNNTTTTACTCTGNACCGCGGNNCCGCATTTNANGATCGNGTTTTTTTTTTTTTTTTTT
  AGGNATAAGTTATTATTAACACTTGCTCCCCAAACAGGATTAGAAAACTATATTGGTAAC
  AGATCTAGAGAATAAAAACACATGCAAACTTTTATGAATGATGAAAGAAATAAAGCCAAT
  CATGACAGGCCTGGCTGAGTGTTCATTATACTCATAAGATTGTCACTGGTTCTGCAAGGT
  GAGCCTCCAGGTAAGTGTTCACTTCTTCTACAGTAGTTAGTTTATCGAAAAAGTTCTCTC
  TTCTTGGGAAGGATGGGACAGAAAGGGGCAGGGTTGGAGTGATTAGGCAATAACATTTTA
  GTTCCATGCACAATCAACCGAGGCAGGGACCAGCTGTCAAAAGGCTCCCAGAAGCCAGGA
  ACCAAAGCCCTCATTGCATGGCACAGATGCTGTGGGGCAGGAACTCCTGCACGTAGGTGG
  CGGCTGCCTTGGAGAGGTAGGTCATGGTGCCAAACCTGCCACTGGGTGCACTGTCATACA
  GAAGAGTACAGATGCCAGATGCGGGATCCTTTGCCAACATGGTATCATCTGCGCCAAGGG
  TTTTCTGTTCTTGAAGGAACAAGTCATTGGCAATATGCACTATTTTTTNCACAGCAATGA
  TGCTTCCGATGCTATGAATTTCAATATCTGCCAGCATGGTGAGGACAAGGATGGCTTCTA
  CCACCAGCTGACGGAACTCTTGCTGAAGTCACGATTCAGGACAGACTCCACATGAACAGA
  GAAATTAATCTCACCTGGAGTTATTTCTTTAGTGGTAGAGGAAAGCAAGGCAAACCCCTT
  AAAAATAAATCCCGGGACACTTTTGCAAAATTTCCCTACTTTTGGCAAAAACCCCCCGGA
  ACTTTTTTAGTGTCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002637
• Insert Size: 4710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002637.1, NP_002628.1
• RefSeq Size: 4215 bp
• RefSeq ORF: 3672 bp
• Locus ID: 5255
• Cytogenetics: Xq13.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).