Phospholipase D1 (PLD1) (NM_002662) Human Untagged Clone

CAT#: SC118496

PLD1 (untagged)-Human phospholipase D1, phosphatidylcholine-specific (PLD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLD1
• Synonyms: CVDD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002662, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCACTGAAAAACGAGCCACGGGTAAATACCTCTGCACTGCAGAAAATTGCTGCTGACATGAGTAATA
  TCATAGAAAATCTGGACACGCGGGAACTCCACTTTGAGGGAGAGGAGGTAGACTACGACGTGTCTCCCAG
  CGATCCCAAGATACAAGAAGTGTATATCCCTTTCTCTGCTATTTATAACACTCAAGGATTTAAGGAGCCT
  AATATACAGACGTATCTCTCCGGCTGTCCAATAAAAGCACAAGTTCTGGAAGTGGAACGCTTCACATCTA
  CAACAAGGGTACCAAGTATTAATCTTTACACTATTGAATTAACACATGGGGAATTTAAATGGCAAGTTAA
  GAGGAAATTCAAGCATTTTCAAGAATTTCACAGAGAGCTGCTCAAGTACAAAGCCTTTATCCGCATCCCC
  ATTCCCACTAGAAGACACACGTTTAGGAGGCAAAACGTCAGAGAGGAGCCTCGAGAGATGCCCAGTTTGC
  CCCGTTCATCTGAAAACATGATAAGAGAAGAACAATTCCTTGGTAGAAGAAAACAACTGGAAGATTACTT
  GACAAAGATACTAAAAATGCCCATGTATAGAAACTATCATGCCACAACAGAGTTTCTTGATATAAGCCAG
  CTGTCTTTCATCCATGATTTGGGACCAAAGGGCATAGAAGGTATGATAATGAAAAGATCTGGAGGACACA
  GAATACCAGGCTTGAATTGCTGTGGTCAGGGAAGAGCCTGCTACAGATGGTCAAAAAGATGGTTAATAGT
  GAAAGATTCCTTTTTATTGTATATGAAACCAGACAGCGGTGCCATTGCCTTCGTCCTGCTGGTAGACAAA
  GAATTCAAAATTAAGGTGGGGAAGAAGGAGACAGAAACGAAATATGGAATCCGAATTGATAATCTTTCAA
  GGACACTTATTTTAAAATGCAACAGCTATAGACATGCTCGGTGGTGGGGAGGGGCTATAGAAGAATTCAT
  CCAGAAACATGGCACCAACTTTCTCAAAGATCATCGATTTGGGTCATATGCTGCTATCCAAGAGAATGCT
  TTAGCTAAATGGTATGTTAATGCCAAAGGATATTTTGAAGATGTGGCAAATGCAATGGAAGAGGCAAATG
  AAGAGATTTTTATCACAGACTGGTGGCTGAGTCCAGAAATCTTCCTGAAACGCCCAGTGGTTGAGGGAAA
  TCGTTGGAGGTTGGACTGCATTCTTAAACGAAAAGCACAACAAGGAGTGAGGATCTTCATAATGCTCTAC
  AAAGAGGTGGAACTCGCTCTTGGCATCAATAGTGAATACACCAAGAGGACTTTGATGCGTCTACATCCCA
  ACATAAAGGTGATGAGACACCCGGATCATGTGTCATCCACCGTCTATTTGTGGGCTCACCATGAGAAGCT
  TGTCATCATTGACCAATCGGTGGCCTTTGTGGGAGGGATTGACCTGGCCTATGGAAGGTGGGACGACAAT
  GAGCACAGACTCACAGACGTGGGCAGTGTGAAGCGGGTCACTTCAGGACCGTCTCTGGGTTCCCTCCCAC
  CTGCCGCAATGGAGTCTATGGAATCCTTAAGACTCAAAGATAAAAATGAGCCTGTTCAAAACCTACCCAT
  CCAGAAGAGTATTGATGATGTGGATTCAAAACTGAAAGGAATAGGAAAGCCAAGAAAGTTCTCCAAATTT
  AGTCTCTACAAGCAGCTCCACAGGCACCACCTGCACGACGCAGATAGCATCAGCAGCATTGACAGCACCT
  CCAGTTATTTTAATCACTATAGAAGTCATCACAATTTAATCCATGGTTTAAAACCCCACTTCAAACTCTT
  TCACCCGTCCAGTGAGTCTGAGCAAGGACTCACTAGACCTCATGCTGATACCGGGTCCATCCGTAGTTTA
  CAGACAGGTGTGGGAGAGCTGCATGGGGAAACCAGATTCTGGCATGGAAAGGACTACTGCAATTTCGTCT
  TCAAAGACTGGGTTCAACTTGATAAACCTTTTGCTGATTTCATTGACAGGTACTCCACGCCCCGGATGCC
  CTGGCATGACATTGCCTCTGCAGTCCACGGGAAGGCGGCTCGTGATGTGGCACGTCACTTCATCCAGCGC
  TGGAACTTCACAAAAATTATGAAATCAAAATATCGGTCCCTTTCTTATCCTTTTCTGCTTCCAAAGTCTC
  AAACAACAGCCCATGAGTTGAGATATCAAGTGCCTGGGTCTGTCCATGCTAACGTACAGTTGCTCCGCTC
  TGCTGCTGATTGGTCTGCTGGTATAAAGTACCATGAAGAGTCCATCCACGCCGCTTACGTCCATGTGATA
  GAGAACAGCAGGCACTATATCTATATCGAAAACCAGTTTTTCATAAGCTGTGCTGATGACAAAGTTGTGT
  TCAACAAGATAGGCGATGCCATTGCCCAGAGGATCCTGAAAGCTCACAGGGAAAACCAGAAATACCGGGT
  ATATGTCGTGATACCACTTCTGCCAGGGTTCGAAGGAGACATTTCAACCGGCGGAGGAAATGCTCTACAG
  GCAATCATGCACTTCAACTACAGAACCATGTGCAGAGGAGAAAATTCCATCCTTGGACAGTTAAAAGCAG
  AGCTTGGTAATCAGTGGATAAATTACATATCATTCTGTGGTCTTAGAACACATGCAGAGCTCGAAGGAAA
  CCTAGTAACTGAGCTTATCTATGTCCACAGCAAGTTGTTAATTGCTGATGATAACACTGTTATTATTGGC
  TCTGCCAACATAAATGACCGCAGCATGCTGGGAAAGCGTGACAGTGAAATGGCTGTCATTGTGCAAGATA
  CAGAGACTGTTCCTTCAGTAATGGATGGAAAAGAGTACCAAGCTGGCCGGTTTGCCCGAGGACTTCGGCT
  ACAGTGCTTTAGGGTTGTCCTTGGCTATCTTGATGACCCAAGTGAGGACATTCAGGATCCAGTGAGTGAC
  AAATTCTTCAAGGAGGTGTGGGTTTCAACAGCAGCTCGAAATGCTACAATTTATGACAAGGTTTTCCGGT
  GCCTTCCCAATGATGAAGTACACAATTTAATTCAGCTGAGAGACTTTATAAACAAGCCCGTATTAGCTAA
  GGAAGATCCCATTCGAGCTGAGGAGGAACTGAAGAAGATCCGTGGATTTTTGGTGCAATTCCCCTTTTAT
  TTCTTGTCTGAAGAAAGCCTACTGCCTTCTGTTGGGACCAAAGAGGCCATAGTGCCCATGGAGGTTTGGA
  CTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002662 unedited
  TGCATTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCAACGCGCA
  GGTGCTAGCGGCCCCTTCGCCCTGCAGCCCCTTTGCTTTTACTCTGTCCAAAGTTAACAT
  GTCACTGAAAAACGAGCCACGGGTAAATACCTCTGCACTGCAGAAAATTGCTGCTGACAT
  GAGTAATATCATAGAAAATCTGGACACGCGGGAACTCCACTTTGAGGGAGAGGAGGTAGA
  CTACGACGTGTCTCCCAGCGATCCCAAGATACAAGAAGTGTATATCCCTTTCTCTGCTAT
  TTATAACACTCAAGGATTTAAGGAGCCTAATATACAGACGTATCTCTCCGGCTGTCCAAT
  AAAAGCACAAGTTCTGGAAGTGGAACGCTTCACATCTACAACAAGGGTACCAAGTATTAA
  TCTTTACACTATTGAATTAACACATGGGGAATTTAAATGGCAAGTTAAGAGGAAATTCAA
  GCATTTTCAAGAATTTCACAGAGAGCTGCTCAAGTACAAAGCCTTTATCCGCATCCCCAT
  TCCCACTAGAAGACACACGTTTAGGAGGCAAAACGTCAGAGAGGAGCCTCGAGAGATGCC
  CAGTTTGCCCCGTTCATCTGAAAACATGATAAGAGAAGAACAATTCCTTGGTAGAAGAAA
  ACAACTGGAAGATTACTTGACAAAGATACTAAAAATGCCCATGTATAGAAACTATCATGC
  CACAACAGAGTTTCTTGATATAAGCCAGCTGTCTTTCATCCATGATTTGGGACCAAAGGG
  CATAGAGTATGATATGAAAAGATCTGGAGACACAGATACCAGCTGAATTGCTGTGTCAGG
  GAGAGCCTGCTACAGATGTCAAAAAGATGTATAGTGAAGATCTTTTATTGTATATGGATC
  AGAACGCGGTGCATTGCCTCGTCTGCTGTAGACAAGATCAATAGTGGAAAGGAACAGAAC
  GAAATTGAATCCGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_002662
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002662.2, NP_002653.1
• RefSeq Size: 5494 bp
• RefSeq ORF: 3225 bp
• Locus ID: 5337
• Cytogenetics: 3q26.31
• Protein Pathways: Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways, Pancreatic cancer, Pathways in cancer
• Gene Summary: 'This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).