PLS1 (NM_002670) Human Untagged Clone

CAT#: SC118504

PLS1 (untagged)-Human plastin 1 (PLS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLS1
• Synonyms: I-PLASTIN; I isoform; Plastin-1; plastin 1; plastin 1 (I isoform)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118504 sequence for NM_002670 edited (data generated by NextGen Sequencing)
  ATGGAAAACAGTACTACTACCATTTCTCGGGAGGAGCTTGAAGAACTACAAGAGGCATTT
  AATAAAATAGATATTGACAATAGTGGGTATGTCAGTGACTATGAACTTCAAGACCTGTTT
  AAGGAAGCAAGCCTTCCTCTGCCTGGCTACAAGGTGCGCGAGATTGTGGAGAAAATTCTA
  TCAGTTGCTGACAGCAACAAAGATGGCAAAATCAGTTTTGAAGAGTTTGTGTCACTAATG
  CAAGAATTAAAAAGCAAAGATATCAGCAAAACATTCCGAAAAATAATTAACAAGAGGGAA
  GGGATTACTGCTATTGGAGGAACTTCAACTATTTCCAGTGAGGGCACACAGCATTCTTAT
  TCAGAGGAAGAAAAAGTGGCTTTTGTTAACTGGATAAACAAAGCCCTGGAGAATGACCCT
  GACTGTAAGCATCTTATACCCATGAATCCCAATGATGATAGTCTTTTCAAGTCACTTGCA
  GATGGCATCCTTCTTTGCAAAATGATCAACTTATCTGAACCAGATACAATTGATGAAAGA
  GCCATCAATAAGAAAAAGCTCACGCCATTCACTATTTCTGAAAATTTAAACCTAGCTCTG
  AATTCTGCCTCAGCCATTGGTTGTACAGTGGTCAACATTGGTGCATCAGATCTCAAAGAA
  GGAAAACCTCACTTGGTCTTGGGACTTCTCTGGCAGATCATCAAAGTTGGCCTTTTTGCT
  GATATTGAGATTTCCAGGAATGAAGCTCTGATTGCATTGTTAAATGAAGGTGAGGAACTA
  GAGGAGCTGATGAAGCTTTCTCCCGAGGAATTACTGCTGCGATGGGTGAACTACCATCTG
  ACCAATGCAGGATGGCATACCATCAGCAACTTCAGCCAAGACATTAAGGACTCGAGAGCC
  TATTTTCATCTGCTTAATCAGATTGCCCCTAAAGGTGGGGAAGATGGACCTGCCATTGCC
  ATTGACCTTTCAGGAATTAATGAGACAAATGACCTGAAGCGTGCTGGACTCATGCTTCAA
  GAAGCAGATAAACTGGGCTGCAAACAGTTTGTTACTCCTGCAGATGTGGTTTCAGGCAAT
  CCTAAACTTAATTTAGCTTTTGTAGCTAATTTGTTTAACACATACCCGTGCCTGCACAAG
  CCGAATAATAATGACATCGATATGAATTTACTGGAAGGAGAGAGCAAGGAAGAGAGAACA
  TTTCGGAACTGGATGAATTCCTTGGGAGTCAACCCATACATTAATCATTTGTACAGTGAC
  CTTGCAGATGCTTTAGTGATCTTTCAGCTCTATGAGATGATCCGAGTGCCAGTCAACTGG
  AGCCATGTCAACAAACCTCCTTATCCTGCCCTTGGAGGGAACATGAAGAAGATTGAAAAC
  TGTAACTATGCAGTGGAACTTGGGAAGAACAAGGCCAAATTCTCCTTGGTTGGCATTGCT
  GGGCAGGACCTAAATGAAGGGAATTCAACACTTACCCTGGCATTGGTATGGCAGCTGATG
  AGAAGGTACACATTGAATGTGTTATCGGATCTTGGAGAGGGTGAAAAAGTAAATGATGAA
  ATTATAATTAAATGGGTCAATCAGACTCTTAAAAGTGCAAACAAAAAGACTTCTATTTCC
  AGCTTCAAGGATAAATCTATAAGCACAAGTTTACCTGTCCTAGATTTAATAGATGCCATT
  GCACCAAATGCAGTTCGTCAAGAAATGATCAGGAGAGAAAACTTATCTGATGAGGACAAG
  CTGAACAATGCTAAATACGCCATTTCAGTTGCTCGAAAGATCGGTGCCCGGATATATGCA
  TTACCTGATGACCTCGTAGAAGTGAAACCAAAGATGGTTATGACGGTGTTTGCATGCTTA
  ATGGGAAAAGGACTGAACAGAATAAAATAA
  
  Clone variation with respect to NM_002670.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002670 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGCGCGGAGAGG
  TAGCCGCAGAGTGGACCTGCAGGTACTTGGATCTCCAGTGGGAGCTGCCCTCTCGAAGGC
  AGGACAGCGGTGGCGGCAGATATAAAGACCTGAAGATAGTCTTTTCTGTCCAAAGATGGA
  AAACAGTACTACTACCATTTCTCGGGAGGAGCTTGAAGAACTACAAGAGGCATTTAATAA
  AATAGATATTGACAATAGTGGGTATGTCAGTGACTATGAACTTCAAGACCTGTTTAAGGA
  AGCAAGCCTTCCTCTGCCTGGCTACAAGGTGCGCGAGATTGTGGAGAAAATTCTATCAGT
  TGCTGACAGCAACAAAGATGGCAAAATCAGTTTTGAAGAGTTTGTGTCACTAATGCAAGA
  ATTAAAAAGCAAAGATATCAGCAAAACATTCCGAAAAATAATTAACAAGAGGGAAGGGAT
  TACTGCTATTGGAGGAACTTCAACTATTTCCAGTGAGGGCACACAGCATTCTTATTCAGA
  GGAAGAAAAAGTGGCTNTTGTTAACTGGATAAACAAAGCCCTGGAGAATGACCCTGACTG
  TAAGCATCTTATACCCATGAATCCCATGATGATAGTCTTTTCAAGTCACTTGCAGATGGC
  ATCCTTCTTTGCANATGATCAACTTATCTGAACCAGATACCATTGATGAAAGAGCCATCA
  ATAAGAAAAGCTCACGCCATTCACTATNTCTGAAAATTTAAACCTAGCTCTGAATTCTGC
  CTCAGCCATNGGNNTGTCACAGTGGTCAACAATGGNTGCATCAGAATCTCAAAGAANGGA
  AACCCTCACTTGGNTCTTGGGACTTCTCTGGCAGATCATCAAGNTGGGCCTTTTTGCTGA
  TATTGAGATTCCAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002670 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATATACACAGCATTTT
  TATTAAATATACTCTGTGAGAAAAAGAACACCCTAAAGTGCCAGATTTCATGTAAATCCA
  ATAACTTAGTAAATACAATGGTGAAAAAAAATCAGAGGGAAACATAATTTAAATAATCTT
  ATAAAAGAGCAAGAACATATCTTAATCTTAAGAAAACAATCATCAAAATATAAAAGGGAA
  AGAAATCTCTTTTGTAGAAGAATATATTTCAAATTCCACCTGTTGATCATTTCATTTAAA
  AAATTCTATTCTAAACACATGGGCAGTCTCTCTTACCAATATATATATTTAAATCAATGG
  TAGAAAGGGAATTTAGACTACTCATTCACACTGAGATAAAGTGACATTTTTGTTGCACAA
  CAGTATTGCTCACTGGCACTAACAGTGGAAGATGGAGAAAGTAGATTTCTGGATTCAATT
  GTTAGCAGCAGAAATGGACTAATGCTAAAAATTTAGAAAAAAATAGTCCAAGCTGTAGCG
  ATTTCCAAGTAATACAAACATTAACCAAACATTGCCAATTTGTAACGGGCTGTGAAAAAC
  ATATGAAAATACTCTACATTGAATACTAGCTTTATGTTACTTTCTATTAAAGATATTCAG
  TGGGCATATTCTAAATTATATAGGGCTTCTATAGAGACCTANACTAGCAAGTATAATATG
  AAGCTAACAGGACAGTGTCAGCATATCTTGGGGATGTCTAGCCAATGGTTGGGCTACAGT
  ATTTGGAAGGGNGTCAATACTATATGCACATTCAAATGAATACTTATAAAAGAAATCTTA
  TGTATCAGATATCACAGNACTGAAAGTGTACACATGTTGTGGGGGGAAATACTGATGGNT
  GGGCTGATGACCCATTAACATACCACTTATGAAAGCTGGNTGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002670
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002670.1, NP_002661.1
• RefSeq Size: 3639 bp
• RefSeq ORF: 1890 bp
• Locus ID: 5357
• Cytogenetics: 3q23
• Domains: CH, EFh
• Gene Summary: 'Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (2) contains an alternate 5' terminal non-coding exon, thus has a different 5' UTR compared to variant 1. Transcript variants 1, 2 and 3 encode the same protein.