PMM1 (NM_002676) Human Untagged Clone

CAT#: SC118505

PMM1 (untagged)-Human phosphomannomutase 1 (PMM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMM1
• Synonyms: PMM 1; PMMH-22; Sec53
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118505 sequence for NM_002676 edited (data generated by NextGen Sequencing)
  ATGGCAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTCCTCTGCCTGTTTGACGTG
  GACGGGACCCTCACGCCGGCTCGCCAGAAAATTGACCCTGAGGTGGCCGCCTTCCTGCAG
  AAGCTACGAAGTAGAGTGCAGATCGGTGTGGTGGGCGGCTCTGACTACTGTAAGATCGCT
  GAGCAGCTGGGTGACGGGGATGAAGTCATTGAGAAGTTTGATTATGTGTTTGCCGAGAAC
  GGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGCAGACCATCCAGAACCACCTG
  GGGGAGGAGCTGCTGCAGGACTTGATCAACTTCTGCCTCAGCTACATGGCCCTGCTCAGG
  CTGCCCAAGAAGCGTGGAACCTTCATCGAGTTCCGGAATGGCATGCTGAACATCTCGCCC
  ATCGGCCGGAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCCGAACTGGACAAGAAAGAG
  AAGATCCGGGAGAAGTTCGTGGAAGCCCTGAAAACAGAGTTTGCTGGCAAAGGGCTGAGG
  TTCTCTCGAGGAGGCATGATCAGCTTTGACGTCTTCCCCGAGGGCTGGGACAAGCGCTAC
  TGCCTGGATAGCCTGGACCAGGACAGCTTCGACACCATCCACTTCTTTGGGAACGAGACT
  AGCCCTGGTGGGAACGACTTTGAGATCTTTGCCGACCCCCGGACTGTTGGCCACAGCGTG
  GTGTCTCCTCAGGACACGGTGCAGCGATGCCGGGAGATTTTCTTCCCAGAGACAGCTCAT
  GAGGCGTGA
  
  Clone variation with respect to NM_002676.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002676 unedited
  CTCTGCATATTTGTCAATACGTACTCCACTATCAGGGGCGCGCCCGCGCAATTCGGCACG
  AGGGCGGTACCTGCAGCCATGGTAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTC
  CTCTGCCTGTTTGACGTGGACGGGACCCTCACGCCGGCTCGCCAGAAAATTGACCCTGAG
  GTGGCCGCCTTCCTGCAGAAGCTACGAAGTAGAGTGCAGATCGGTGTGGTGGGCGGCTCT
  GACTACTGTAAGATCGCTGAGCAGCTGGGTGACGGGGATGAAGTCATTGAGAAGTTTGAT
  TATGTGTTTGCCGAGAACGGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGCAG
  ACCATCCAGAACCACCTGGGGGACGAGCTGCTGCAGGACTTGATCAACTCCTGCCTCAAT
  TACATGGCCCTGTTAAGGCTGCCAAAAAACGCGGAACCTTCATCGAGTCCCGAAAGGCTG
  GCTTAAAATTTTCCCTACGGGCGGGGTTGCCCCTGGGGGAAAAGGATCAAGTTTTCCAAC
  CGGCCAAAAAAAAAAAACCCGGCAAAAATCGGGAAACCCTAAAAAAAAAATTTTCGCGAA
  AGGGCGGGGCTTTTCCCGGGGGGCATAAACCCCTTCTACCCCCCCCGGGAGGGGCGAAAA
  CCCTCTTTTGTGTAATATGCCAACAAAAAAATCTCCACCCCCTCCCTCTTTGTGGAAAAA
  ACACCCCCTGGGGAGACAAACTTAATATTTTCCCCCCCCCGCCGTGTGGACACCGGGGGT
  TTTTCTCCTCCAATGCCCCCCCCGGGAACTCTCTTCCCTAAAACCACCCAGGAGGGGGCG
  CCCCCCCCGTGGCCGCCCCTTTCAAAAACTCCCCCCCAAAACCGGGGGTGCGCAAACCGG
  CCCCCCTCTCCCGTGACGTCCCCCCACCCCAGCGAGCGGGGCCCGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002676 unedited
  CTCTCTTTTTTCTTTTTTCTTTTCCTTTGTTCCTGGGAAACTTTAATACTGTGACAAAGT
  TCTCTAAAATAGGCACCTTCCCCACCGTACCTCATCGCCCAGGGCAGGCAGGCAGGGCAG
  GCTAAATCTCGTACCGATACTTGAGCACGCCTCCTCCTGGTGCAAAAAGAAACCTCTTCT
  GTACCGAAATACAAGCAGCAGCTGTGGCCTGGGCCACCAGGTGGAGCATGGGGAACACTC
  TGGGCCCTGGGAGGACGAAGCCAGTGCCACTAGGAGCAGACTGGCTGGGGACGGTTGTCC
  ACACAGACTCTGGCCCCATCTGGGTGGGCTTGCAGCAGGCGTCCTGGGCCAGAGGAGGGG
  CCCTGGCATCTATCCAACACCAGGACCTCTCTTTAGGCCTAGGCCAAACTCTTCAGAAGT
  CACGACACACAGATGTGGGCCCCGGTCACGCCTCATGAGCTGTCTCTGGGAAGAAAATCT
  CCCGGCATCGCTGCACCGTGTCCTGAGGAGACACCACGCTGTGGCCAACAGTCCGGGGGT
  CGGCAAAGATCTCAAAGTCGTTCCCACCAGGGCTAGTCTCGTTCCCAAAGAAGTGGATGG
  TGTCGAAGCTGTCCTGGTCCAGGCTATCCAGGCAGTAGCGCTTGTCCCAGCCCTCGGGGA
  AGACGTCAAAGCTGATCATGCCTCCTCGAGAAACCTCAGCCCTTTGCCAGCAAACTCTGT
  TTTCAGGGCTTCCACGAACTTCTCCCGGATCTTCTCTTTCTTGTCCAGTTCGGAGAACTC
  GATCCTCTCCTCCAGGTGCANCTCCGGCCGATGGNCGAGATGTTCANCATGCCCTTCCCG
  GACTCGATGAAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002676
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002676.1, NP_002667.1
• RefSeq Size: 1210 bp
• RefSeq ORF: 789 bp
• Locus ID: 5372
• Cytogenetics: 22q13.2
• Domains: PMM
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: 'Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008]'