PMM1 (NM_002676) Human Untagged Clone

CAT#: SC322342

PMM1 (untagged)-Human phosphomannomutase 1 (PMM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMM1
• Synonyms: PMM 1; PMMH-22; Sec53
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322342 CGCGGACCTGCAGCCATGGCAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTCCTC
  TGCCTGTTTGACGTGGACGGGACCCTCACGCCGGCTCGCCAGAAAATTGACCCTGAGGTG
  GCCGCCTTCCTGCAGAAGCTACGAAGTAGAGTGCAGATCGGTGTGGTGGGCGGCTCTGAC
  TACTGTAAGATCGCTGAGCAGCTGGGTGACGGGGATGAAGTCATTGAGAAGTTTGATTAT
  GTGTTTGCCGAGAACGGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGCAGACC
  ATCCAGAACCACCTGGGGGAGGAGCTGCTGCAGGACTTGATCAACTTCTGCCTCAGCTAC
  ATGGCCCTGCTCAGGCTGCCCAAGAAGCGTGGAACCTTCATCGAGTTCCGGAATGGCATG
  CTGAACATCTCGCCCATCGGCCGGAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCCGAA
  CTGGACAAGAAAGAGAAGATCCGGGAGAAGTTCGTGGAAGCCCTGAAAACAGAGTTTGCT
  GGCAAAGGGCTGAGGTTCTCTCGAGGAGGCATGATCAGCTTTGACGTCTTCCCCGAGGGC
  TGGGACAAGCGCTACTGCCTGGATAGCCTGGACCAGGACAGCTTCGACACCATCCACTTC
  TTTGGGAACGAGACTAGCCCTGGTGGGAACGACTTTGAGATCTTTGCCGACCCCCGGACT
  GTTGGCCACAGCGTGGTGTCTCCTCAGGACACGGTGCAGCGATGCCGGGAGATTTTCTTC
  CCAGAGACAGCTCATGAGGCGTGACCGGGGCCCACATCTGTGTGTCGTGACTTCTGAAGA
  GTTTGGCCTAGGCCTAAAGAGAGGTCCTGGTGTTGGATAGATGCCAGGGCCCCTCCTCTG
  GCCCAGGACGCCTGCTGCAAGCCCACCCAGATGGGGCCAGAGTCTGTGTGGACAACCGTC
  CCCAGCCAGTCTGCTCCTAGTGGCACTGGCTTCGTCCTCCCAGGGCCCAGAGTGTTCCCC
  ATGCTCCACCTGGTGGCCCAGGCCACAGCTGCTGCTTGTATTTCGGTACAGAAGAGGTTT
  CTTTCTGCACCAGGAGGAGGCGTGCTCAAGTATCGGTACGAGATCTAGCCTGCCCTGCCT
  GCCTGCCCTGGGCGATGAGGTACGGTGGGGAAGGTGCCTATTTTAGAGAACTTTGTCACA
  GTATTAAAGTTCCCAGAACAAAGTAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002676
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002676.1, NP_002667.1
• RefSeq Size: 1210 bp
• RefSeq ORF: 789 bp
• Locus ID: 5372
• Cytogenetics: 22q13.2
• Domains: PMM
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: 'Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008]'