PFKP (NM_002627) Human Untagged Clone

CAT#: SC118507

PFKP (untagged)-Human phosphofructokinase, platelet (PFKP), transcript variant 1


  "NM_002627" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PFKP
Synonyms ATP-PFK; PFK-C; PFK-P; PFKF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118507 sequence for NM_002627 edited (data generated by NextGen Sequencing)
ATGGACGCGGACGACTCCCGGGCCCCCAAGGGCTCCTTGCGGAAGTTCCTGGAGCACCTC
TCCGGGGCCGGCAAGGCCATCGGCGTGCTGACCAGCGGCGGGGATGCTCAAGGTATGAAC
GCTGCCGTCCGTGCCGTGGTGCGCATGGGTATCTACGTGGGGGCCAAGGTGTACTTCATC
TACGAGGGCTACCAGGGCATGGTGGACGGAGGCTCAAACATCGCAGAGGCCGACTGGGAG
AGTGTCTCCAGCATCCTGCAAGTGGGCGGGACGATCATTGGCAGTGCGCGGTGCCAGGCC
TTCCGCACGCGGGAAGGCCGCCTGAAGGCTGCTTGCAACCTGCTGCAGCGCGGCATCACC
AACCTGTGTGTGATCGGCGGGGACGGGAGCCTCACCGGGGCCAACCTCTTCCGGAAGGAG
TGGAGTGGGCTGCTGGAGGAGCTGGCCAGGAACGGCCAGATCGATAAGGAGGCCGTGCAG
AAGTACGCCTACCTCAACGTGGTGGGCATGGTGGGCTCCATCGACAATGATTTCTGCGGC
ACCGACATGACCATCGGCACGGACTCCGCCCTGCACAGGATCATCGAGGTCGTCGACGCC
ATCATGACCACGGCCCAGAGCCACCAGAGGACCTTCGTTCTGGAGGTGATGGGACGACAC
TGTGGGTACCTGGCCCTGGTGAGTGCCTTGGCCTGCGGTGCGGACTGGGTGTTCCTTCCA
GAATCTCCACCAGAGGAAGGCTGGGAGGAGCAGATGTGTGTCAAACTCTCGGAGAACCGT
GCCCGGAAAAAAAGGCTGAATATTATTATTGTGGCTGAAGGAGCAATTGATACCCAAAAT
AAACCCATCACCTCTGAGAAAATCAAAGAGCTTGTCGTCACGCAGCTGGGCTATGACACA
CGTGTGACCATCCTCGGGCACGTGCAGAGAGGAGGGACCCCTTCGGCATTTGACAGGATC
TTGGCCAGCCGCATGGGAGTGGAGGCAGTCATCGCCTTGCTAGAGGCCACCCCGGACACC
CCAGCTTGCGTCGTGTCACTGAACGGGAACCACGCCGTGCGCCTGCCGCTGATGGAGTGC
GTGCAGATGACTCAGGATGTGCAGAAGGCGATGGACGAGAGGAGATTTCAAGATGCGGTT
CGACTCCGAGGGAGGAGCTTTGCGGGCAACCTGAACACCTACAAGCGACTTGCCATCAAG
CTGCCGGATGATCAGATCCCAAAGACCAATTGCAACGTAGCTGTCATCAACGTGGGGGCA
CCCGCGGCTGGGATGAACGCGGCCGTACGCTCAGCTGTGCGCGTGGGCATTGCCGACGGC
CACAGGATGCTCGCCATCTATGATGGCTTTGACGGCTTCGCCAAGGGCCAGATCAAAGAA
ATCGGCTGGACAGATGTCGGGGGCTGGACCGGCCAAGGAGGCTCCATTCTTGGGACAAAA
CGCGTTCTCCCGGGGAAGTACTTGGAAGAGATCGCCACACAGATGCGCACGCACAGCATC
AACGCGCTGCTGATCATCGGTGGATTCGAGGCCTACCTGGGACTCCTGGAGCTGTCAGCC
GCCCGGGAGAAGCACGAGGAGTTCTGTGTCCCCATGGTCATGGTTCCCGCTACTGTGTCC
AACAATGTGCCGGGTTCCGATTTCAGCATCGGGGCAGACACCGCCCTGAACACTATCACC
GACACCTGCGACCGCATCAAGCAGTCCGCCAGCGGAACCAAGCGGCGCGTGTTCATCATC
GAGACCATGGGCGGCTACTGTGGCTACCTGGCCAACATGGGGGGGCTCGCGGCTGGAGCT
GATGCCGCATACATTTTCGAAGAGCCCTTCGACATCAGGGATCTGCAGTCCAACGTGGAG
CACCTGACGGAGAAAATGAAGACCACCATCCAGAGAGGCCTTGTGCTCAGAAATGAGAGC
TGCAGTGAAAACTACACCACCGACTTCATTTACCAGCTGTATTCAGAAGAGGGCAAAGGC
GTGTTTGACTGCAGGAAGAACGTGCTGGGTCACATGCAGCAGGGTGGGGCACCCTCTCCA
TTTGATAGAAACTTTGGAACCAAAATCTCTGCCAGAGCTATGGAGTGGATCACTGCAAAA
CTCAAGGAGGCCCGGGGCAGAGGAAAAAAATTTACCACCGATGATTCCATTTGTGTGCTG
GGAATAAGCAAAAGAAACGTTATTTTTCAACCTGTGGCAGAGCTGAAGAAGCAAACGGAT
TTTGAGCACAGGATTCCCAAAGAACAGTGGTGGCTCAAGCTACGGCCCCTCATGAAAATC
CTGGCCAAGTACAAGGCCAGCTATGACGTGTCGGACTCAGGCCAGCTGGAACATGTGCAG
CCCTGGAGTGTCTGA

Clone variation with respect to NM_002627.4
951 c=>t;1281 a=>g;1794 c=>t
>OriGene 5' read for NM_002627 unedited
GTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCCCATTGCC
TGCTGCGCACCCGGACGTGCGGCTCCCCTCGGCCTCCTCGCCATGGACGCGGACGACTCC
CGGGCCCCCAGGGCTCCTTGCGGAAGTTCCTGGAGCACCTCTCCGGGGCCGGCAAGGCCA
TCGGCGTGCTGACCAGCGGCGGGGATGCTCAAGGTATGAACGCTGCCGTCCGTGCCGTGG
TGCGCATGGGTATCTACGTGGGGGCCAAGGTGTACTTCATCTACGAGGGCTACCAGGGCA
TGGTGGACGGAGGCTCAAACATCGCAGAGGCCGACTGGGAGAGTGTCTCCAGCATCCTGC
AAGTGGGCGGGACGATCATTGGCAGTGCGCGGTGCCAGGCCTTCCGCACGCGGGAAGGCC
GCCTGAAGGCTGCTTGCAACCTGCTGCAGCGCGGCATCACCAACCTGTGTGTGATCGGCG
GGGACGGGAGCCTCACCGGGGCCAACCTCTTCCGGAAGGAGTGGAGTGGGCTGCTGGAGG
AGCTGGCCAGGAACGGCCAGATCGATAAGGAGGCCGTGCAGAAGTACGCCTACCTCAACG
TGGTGGGCATGGTGGGCTCCATCGACAATGATTTCTGCGGCACCGACATGACCATCGGCA
CGGACTCCGCCCTGCACAGGATCATCGAGGTCGTCGACGCCATCATGACCACGGCCCAGA
GCCACCAGAGGACCTTCGTTCTGGNAGGTGATGGGACGACACTGTGGGTACCTGGCCCTG
GTGAGTGCCTTGGCCTGCGGTGCGACTCTGGTTGTTCCTTNNCAGAATCTCCACCANAGA
ANGNCTGGGAGGAGCANNATGTGTGTCAAACTCTCGGAGAACCGTGCCCGGAAAAAAGGC
TGATTTTATATTGGG
>OriGene 3' read for NM_002627 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTTGTAGGCAAATGTTTA
ATTCTGCTCATATGCACATCTGAAAGCATGAGACACACTCCACAGACAGCACGCACTGGG
GCTGGTGGGGCAGATGGGCACTCGCCGATTAGGTATTAATGTTAATAATACGTGCATAAA
GTGCTGATAAAATAACTTAAGTGTTACAAAAACAGACAGTCCACGGTGGCTGCAGGCACA
TGCAGGCGGGACTGGGTCAGACACTCCAGGGCTGCACATGTTCCAGCTGGCCTGAGTCCG
ACACGTCATAGCTGGCCTTGTACTTGGCCAGGATTTTCATGAGGGGCCGTAGCTTGAGCC
ACCACTGTTCTTTGGGAATCCTGTGCTCAAAATCCGTTTGCTTCTTCAGCTCTGCCACAG
GTTGAAAAATAACGTTTCTTTTGCTTATTCCCAGCACACAAATGGAATCATCGGTGGTAA
ATTTTTTTCCTCTGCCCCGGGCCTCCTTGAGTTTTGCAGTGATCCACTCCATAGCTCTGG
CAGAGATTTTGGTTCCAAAGTTTCTATCAAATGGAGAGGGTGCCCCACCCTGCTGCATGT
GACCCAGCACGTTCTTCCTGCAGTCAAACACGCCTTTGCCCTCTTCTGAATACAGCTGGT
AAATGAAGTCGGTGGTGTAGTTTTCACTGCAGCTCTCATTTCTGAGCACAAGGCCTCTCT
GGATGGTGGTCTTCATTCTCTCCGTCACGTGCTCCACGTTTGACTGCACATCCCTGATGT
CNAAAGGCTCTTCCAAATGTATGCGGCATCAGCTCCAGCCGCGAGCCCCCCCATTTTGGC
CAAGTACCCCAGTTACCGCCCATGTTCTTNATGATGACACCCCCGCTTGGTTCCCTTGCC
GACTGTTTGATCCGTCCAGGTGTCGGAAATTGTTAGGCCGTGTTTGCCCGATGTGAAATC
GAACCGGCAAT
Restriction Sites NotI-NotI     
ACCN NM_002627
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002627.3, NP_002618.1
RefSeq Size 2628 bp
RefSeq ORF 2355 bp
Locus ID 5214
Cytogenetics 10p15.2
Domains PFK
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
Gene Summary 'This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]'
Transcript Variant: This variant (1) encodes the longest isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.