DNA Polymerase beta (POLB) (NM_002690) Human Untagged Clone

CAT#: SC118518

POLB (untagged)-Human polymerase (DNA directed), beta (POLB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLB
• Synonyms: MGC125976
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118518 sequence for NM_002690 edited (data generated by NextGen Sequencing)
  ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
  GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
  AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
  AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
  AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
  ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
  ACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG
  AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGAT
  ATTGTACTAAATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGT
  TTCAGAAGAGGTGCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTC
  ACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAG
  GTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAG
  CTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATA
  CCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG
  AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCC
  TTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTT
  GATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
  
  Clone variation with respect to NM_002690.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002690 unedited
  CCCCTGTTAGAGTTGAATCTCGACTCATATAGGCGGCCGCTGAACGGCACGAGGCCGGCA
  CTGGGTTGCTCCTGCTCCCGTCTCCAAGTCCTGGTACCTCCTTCAAGCTGGGAGAGGGCT
  CTAGTCCCTGGTTCTGAACACTCTGGGGTTCTCGGGTGCAGGCCGCCATGAGCAAACGGA
  AGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACAGAACTCGCAAACT
  TTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGAAAAGCAGCATCTG
  TTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAGAAATTGCCTGGAG
  TAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGAAAATTACGTAAAC
  TGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTGACTCGAGTTAGTG
  GCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAAACACTAGAAGATC
  TCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGGG
  ACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAATG
  AAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTG
  CAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCACCCAGCTTCACTTCAGAATCAAC
  CAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGCTACAAAGGGTTCATTTATCACA
  CATACCCCTGCCAAGGGTGAGACAAAGCTCATGGGTGTTTGCCAGCTTCCCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002690 unedited
  AACCTTCCAGGGCCAGGNANAGCACTGGGGNAGGGGGTCACAGGGNATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTAATAGTACATGAGGTTTTATTGACTACATTGCAAGCAAAAGCATGAAGAAGAAACAA
  TCATTTAAAAACACCAAAGACCCTTACATAGCAAAGGTTAAGAAATAAATTAAGACTCCT
  ATTGGGTTGTGTCTGCCAGGGAGGATACAGGCCTCATTCGCTCCGGTCCTTGGGTTCCCG
  GTATTTCCACTGGATGTAATCAAAGATGTCTTTTTCACTATCCACTGGCAGGGGTTCTCC
  TGCAACTCCAGTGACTCCCAAGGGACGGATGGTGTACTCATTGATTGTGAAACCCTTTTC
  TAGGGCATGAGCCCTCATATTCTTATTGAAAATATCACTCCCAGTGAAATAGAGAACACC
  ACAGTAATACTGATCTTTGGGTATCAACCTGATATCAATTCTTCTGTGTGGATATTCTTT
  TTCATCATTTTTACTGGGAAGCTGGCAAACACCCATGAACTTTGTCTCACCCTTTGACAG
  GGTATCTGTGATAAAATGAACCTTTTGTAACTGCTCCACAACCTGATGTAACAGTTTTGG
  CTGTTTGGTTGATTCTGAAGTGAAGCTGGGATGGGTCAGGAGAACATCCATGTCACCACT
  GGACTCTGCACCTCTTCTGAAACTGCCACAGACTGTAGCAATGTATTCAGAATCCACTTT
  TTTAACTTCATTTAGTACAATATCTTGCATTTGTAACATCTCTTCACGAGGAATNCTTTT
  NTNCAAGTCCCCAAATATNTCAGCCCAATTCGCTGAGATGGTCAATTATCTTCATTTTNC
  TGAATCTTCTAGGTTTAATTCTTATTAACAACTNCTGCAGAGAGGCCATGCCCTACTCCG
  TCAGAATGATGATGACTCNTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002690
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002690.1, NP_002681.1
• RefSeq Size: 1259 bp
• RefSeq ORF: 1008 bp
• Locus ID: 5423
• Cytogenetics: 8p11.21
• Domains: POLXc, HhH1
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: 'The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]'