DNA Polymerase beta (POLB) (NM_002690) Human Tagged ORF Clone

CAT#: RG210765

POLB (GFP-tagged) - Human polymerase (DNA directed), beta (POLB)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLB
• Synonyms: MGC125976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210765 representing NM_002690
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACAGAACTCGCAA
  ACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGAAAAGCAGCATCTGTTATAGC
  AAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAGAAATTGCCTGGAGTAGGAACAAAAATTGCT
  GAAAAGATTGATGAGTTTTTAGCAACTGGAAAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGA
  GTTCATCCATCAATTTCCTGACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGA
  AGGAATTAAAACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG
  AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAA
  ATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTGCAGAGTC
  CAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCAACCAAACAGCCAAAACTG
  TTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAA
  AGTTCATGGGTGTTTGCCAGCTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATAT
  CAGGTTGATACCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG
  AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCA
  CTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAGTGGAAATA
  CCGGGAACCCAAGGACCGGAGCGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210765 representing NM_002690
  Red=Cloning site Green=Tags(s)
  
  MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIA
  EKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGL
  KYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKL
  LHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK
  NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002690
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002690.1, NP_002681.1
• RefSeq Size: 1259 bp
• RefSeq ORF: 1008 bp
• Locus ID: 5423
• Cytogenetics: 8p11.21
• Domains: POLXc, HhH1
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: 'The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]'