PBX1 (NM_002585) Human Untagged Clone

CAT#: SC118523

PBX1 (untagged)-Human pre-B-cell leukemia homeobox 1 (PBX1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PBX1
• Synonyms: CAKUHED
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118523 sequence for NM_002585 edited (data generated by NextGen Sequencing)
  ATGGACGAGCAGCCCAGGCTGATGCATTCCCATGCTGGGGTCGGGATGGCCGGACACCCC
  GGCCTGTCCCAGCACTTGCAGGATGGGGCCGGAGGGACCGAGGGGGAGGGCGGGAGGAAG
  CAGGACATTGGAGACATTTTACAGCAAATTATGACCATCACAGACCAGAGTTTGGATGAG
  GCGCAGGCCAGAAAACATGCTTTAAACTGCCACAGAATGAAGCCTGCCTTGTTTAATGTG
  TTGTGTGAAATCAAAGAAAAAACAGTTTTGAGTATCCGAGGAGCCCAGGAGGAGGAACCC
  ACAGACCCCCAGCTGATGCGGCTGGACAACATGCTGTTAGCGGAAGGCGTGGCGGGGCCT
  GAGAAGGGCGGAGGGTCGGCGGCAGCGGCGGCAGCGGCGGCGGCTTCTGGAGGGGCAGGT
  TCAGACAACTCAGTGGAGCATTCAGATTACAGAGCCAAACTCTCACAGATCAGACAAATC
  TACCATACGGAGCTGGAGAAATACGAGCAGGCCTGCAACGAGTTCACCACCCACGTGATG
  AATCTCCTGCGAGAGCAAAGCCGGACCAGGCCCATCTCCCCAAAGGAGATTGAGCGGATG
  GTCAGCATCATCCACCGCAAGTTCAGCTCCATCCAGATGCAGCTCAAGCAGAGCACGTGC
  GAGGCGGTGATGATCCTGCGTTCCCGATTTCTGGATGCGCGGCGGAAGAGACGGAATTTC
  AACAAGCAAGCGACAGAAATCCTGAATGAATATTTCTATTCCCATCTCAGCAACCCTTAC
  CCCAGTGAGGAAGCCAAAGAGGAGTTAGCCAAGAAGTGTGGCATCACAGTCTCCCAGGTA
  TCAAACTGGTTTGGAAATAAGCGAATCCGGTACAAGAAGAACATAGGTAAATTTCAAGAG
  GAAGCCAATATTTATGCTGCCAAAACAGCTGTCACTGCTACCAATGTGTCAGCCCATGGA
  AGCCAAGCTAACTCGCCCTCAACTCCCAACTCGGCTGGTTCTTCCAGTTCTTTTAACATG
  TCAAACTCTGGAGATTTGTTCATGAGCGTGCAGTCACTCAATGGGGATTCTTACCAAGGG
  GCCCAGGTTGGAGCCAACGTGCAATCACAGGTGGATACCCTTCGCCATGTTATCAGCCAG
  ACAGGAGGATACAGTGATGGACTCGCAGCCAGTCAGATGTACAGTCCGCAGGGCATCAGT
  GCTAATGGAGGTTGGCAGGATGCTACTACCCCTTCATCAGTGACCTCCCCTACAGAAGGC
  CCTGGCAGTGTTCACTCTGATACCTCCAACTGA
  
  Clone variation with respect to NM_002585.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002585 unedited
  GGGTGCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCTTGAA
  GGATAAAAAGCCTTGGTGCTTCCCAGGAGCCGAGCCGAGGAGCAGAAGAGGAAGAGCCGG
  GGGCTGCCGTAGCCTTTGGAGATGGACGAGCAGCCCAGGCTGATGCATTCCCATGCTGGG
  GTCGGGATGGCCGGACACCCCGGCCTGTCCCAGCACTTGCAGGATGGGGCCGGAGGGACC
  GAGGGGGAGGGCGGGAGGAAGCAGGACATTGGAGACATTTTACAGCAAATTATGACCATC
  ACAGACCAGAGTTTGGATGAGGCGCAGGCCAGAAAACATGCTTTAAACTGCCACAGAATG
  AAGCCTGCCTTGTTTAATGTGTTGTGTGAAATCAAAGAAAAAACAGTTTTGAGTATCCGA
  GGAGCCCAGGAGGAGGAACCCACAGACCCCCAGCTGATGCGGCTGGACAACATGCTGTTA
  GCGGAAGGCGTGGCGGGGCCTGAGAAGGGCGGAGGGTCGGCGGCAGCGGCGGCAGCGGCG
  GCGGCTTCTGGAGGGGCAGGTTCAGACAACTCAGTGGAGCATTCAGATTACAGAGCCAAA
  CTCTCACAGATCAGACAAATCTACCATACGGAGCTGGAGAAATACGAGCAGGCCTGCAAC
  GAGTTCACCACCCACGTGATGAATCTCCTGCGAGAGCAAGCCNGACCAGGCCCATCTCCC
  CAAAGGAGATTGAGCGGATGGTCAGCATCATCCACCGCAAGTTCAGCTCCATCCAGATGC
  AGCTCAAGCAGAGCACGTGCGAAGCGGTGATGATCCTGCNGTCCCGATTTCTGGATGCGN
  CGCNGNAGAGACGGNATTTCAACAGCAGCGACAGAAATCCTGAATGAATATTNTATTCCC
  ATCTCAGCACCCTTACCCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002585 unedited
  NNNGGGATATATCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTT
  TTTTAGTTTACAAAATGAATTACTGTCACTTTTAAACATTGTGAAACAAGAAATGGATGT
  GCACAACTCGACACTTTTCTAGCATTCTTGAACTAATTCACAAATGCAAGAAAATAAAAG
  AAAAATGAGGGAAATGATGAATGTAGGTAGTCTGGTGTTAAAAACTAATGCAGGAATGAT
  GCGCATTGTCACAGAAAGAAGAGGGGAAATTCCCCATCCTGTTTTGCGTGCTATGAGGGT
  CATTTTTAAAATTTTTATTATAAACACTATTAAATTCAGACCGCTTTTTTGGTTTTTTTT
  TTCTTTATCTGAATATACAAGAACATTCATTATCAAATGTTCATCATCAATACTACAAAG
  AACGAGACACAAGTCGCAAGAAACAATGGAAAATGTTAATAAAGCTGAAAGAATCGTTGA
  GTATTTTTTTGTTTCTTTTAAAGTTTTTTTTAAATTTGAGTTTCTGTAGTTTCATCTTTT
  TTGGTATCGAAGTCAGGTTTTTCTGGGCAGAAAAACAAAGAGCAGGAAAGAGAGAGACAG
  AGAGAAAGATGTGTGAGAAAAGACAACCAGCAAATAAAGAATCACCACCATAGCAAAATA
  AAACATAAATTGAAAGAAGGTGCCACTTGGCAAATGTACTGCACGGGCGTCAATTCCATG
  CCAGAGGGAGGATGAATTAGGGATGAAAAGGAGGAGATAAGGAAGAGTTACAAGGTGGGG
  CAAACCCANGGTCTAGTATCGTTTCTCAGGGGAAAATCGCTGTCGGGGAAAATGGAGCAC
  TCCCACCTCTATTAGAAAATGAGAAAATCCTGGGTGCATCTTGCTCTCTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002585
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002585.1, NP_002576.1
• RefSeq Size: 1819 bp
• RefSeq ORF: 1293 bp
• Locus ID: 5087
• Cytogenetics: 1q23.3
• Domains: homeobox, PBX
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: 'This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1, also known as isoform PBX1a).