PCNA (NM_002592) Human Untagged Clone

CAT#: SC118528

PCNA (untagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCNA
• Synonyms: ATLD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118528 sequence for NM_002592 edited (data generated by NextGen Sequencing)
  ATGTTCGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAG
  GACCTCATCAACGAGGCCTGCTGGGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATG
  GACTCGTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTAC
  CGCTGCGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAA
  TGCGCCGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCG
  CTAGTATTTGAAGCACCAAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGAT
  TTAGATGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCT
  TCTGGTGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATT
  TCCTGTGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGAAATGGAAACATT
  AAATTGTCACAGACAAGTAATGTCGATAAAGAGGAGGAAGCTGTTACCATAGAGATGAAT
  GAACCAGTTCAACTAACTTTTGCACTGAGGTACCTGAACTTCTTTACAAAAGCCACTCCA
  CTCTCTTCAACGGTGACACTCAGTATGTCTGCAGATGTACCCCTTGTTGTAGAGTATAAA
  ATTGCGGATATGGGACACTTAAAATACTACTTGGCTCCCAAGATCGAGGATGAAGAAGGA
  TCTTAG
  
  Clone variation with respect to NM_002592.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002592 unedited
  NGGGTCAGCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCGCT
  TGCGGACGCGGCGGCATTAAACGGTTGCAGGACCAGCAGAGTGGTCGTTGTCTTTCTAGG
  TCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCTCTGAGGCTCCTGAAGCCGAAACCAG
  CTAGACTTTCCTCCTTCCCGCCTGCCTGTAGCGGCGTTGTTGCCACTCCGCCACCATGTT
  CGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAGGACCT
  CATCAACGAGGCCTGCTGCGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATGGACTC
  GTCCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTACCGCTG
  CGACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAATGCGC
  CGGCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCGCTAGT
  ATTTGAAGCACCCAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGATTTAGA
  TGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCTTCTGG
  TGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATTTCCTG
  CGCAAAAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGGAATGGAAACATTAAATT
  GTCACAGACAAGTAATGTCGATAAAAGANAGGAACCTGTTACCATAGAGATGAATGAACC
  AGTNTCACTAACTTTTGCACTGGAGTACCTGAACTTCTTTACAAAGCCACTCCACTCTCT
  TCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002592 unedited
  CGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATGTTTGAAATTCAAGT
  AACTTTATTTAAATTCAAAAACAATTCTTAAAACTGCATTTAGAGTCAAGACCCTTTTGT
  ATTATAAAAATCACAAGTATTTCTAAGAGACAAAAATACTTCTAGGTTAACTAGACCAGA
  TCTGACTTTGGACTTTATTCTTTAAACAAATTGCAGAGAATAGAGAAAAAAATAGGTTAT
  TTACAGAAAACAATATCTACATATGTACTTAGAGGTACAAATTTGGTGACAGAAAAGACT
  TCAGTATATGCTGGCATCTTAGAAGCAGTTCTCAAAGAGCTTAGTTTTATTTTCTTGAAT
  TTTAAGAATGCCTAAGATCCTTCTTCATCCTCGATCTTGGGAGCCAAGTAGTATTTTAAG
  TGTCCCATATCCGCAATTTTATACTCTACAACAAGGGGTACATCTGCAGACATACTGAGT
  GTCACCGTTGAAGAGAGTGGAGTGGCTTTTGTAAAGAAGTTCAGGTACCTCAGTGCAAAA
  GTTAGTTGAACTGGTTCATTCATCTCTATGGTAACAGCTTCCTCCTCTTTATCGACATTA
  CTTGTCTGTGACAATTTAATGTTTCCATTTCCAAGNTCTCCACTTGCAGAAATTTCACTC
  CGTCTTTTGCACAGGAAATTACCACAGCATCTCCAATATGCCTGAGATCTCGGCATATAC
  GTGCAAATTCACCACGAGGCATCTTTACTACACAGCTGTACCTCTGGTCTGAAATTCCAA
  GTTGTTAACATCTAAATCCATCCACTTCATTCATAGCCTGAAAACTTCTCTGGTTTGGGG
  CCTTAAAAACTACCGCCAAGTATCCCGGTATCTCCGGCCCTTAGGGATGAAACCTCATTG
  CCGGCCTTTTAAATTTTGGCTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002592
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002592.2, NP_002583.1
• RefSeq Size: 1355 bp
• RefSeq ORF: 786 bp
• Locus ID: 5111
• Cytogenetics: 20p12.3
• Domains: PCNA
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.