PDE6D (NM_002601) Human Untagged Clone

CAT#: SC118535

PDE6D (untagged)-Human phosphodiesterase 6D, cGMP-specific, rod, delta (PDE6D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE6D
• Synonyms: JBTS22; PDED
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002601 edited
  ATGTCAGCCAAGGACGAGCGGGCCAGGGAGATCCTGAGGGGCTTCAAACTAAATTGGATG
  AACCTTCGGGATGCTGAGACAGGGAAGATACTCTGGCAAGGAACAGAAGACCTGTCTGTC
  CCTGGTGTGGAGCATGAAGCCCGTGTTCCCAAGAAAATCCTCAAGTGCAAGGCAGTGTCT
  CGAGAACTTAATTTTTCTTCGACAGAACAAATGGAAAAATTCCGCCTGGAACAAAAAGTT
  TACTTCAAAGGGCAATGCCTAGAAGAATGGTTCTTCGAGTTTGGCTTTGTGATCCCTAAC
  TCCACAAATACCTGGCAGTCCTTGATAGAGGCAGCACCCGAGTCCCAGATGATGCCAGCA
  AGCGTCTTAACTGGGAACGTTATCATAGAAACAAAGTTTTTTGACGACGATCTTCTTGTA
  AGCACATCCAGAGTGAGACTTTTCTATGTTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002601 unedited
  TTTGTACTACGAACTCACTATAGGGCGGCCGCGACATTCGCACGAGGGAGAGAGAGGCCG
  GTCCTGGTCTGGCCGCTGCGGCTGCTAGCCCGAGGTCTCCAAGCCGGGCTGCGGCTCCAT
  CCTCGGCTCCTGGGCACCGTCTGCGAGGCTCCGCCGACCAGAGTGAGAAAGCCGCGGGCG
  GCGACCGCCGCATCATGTCAGCCAAGGACGAGCGGGCCAGGGAGATCCTGAGGGGCTTCA
  AACTAAATTGGATGAACCTTCGGGATGCTGAGACAGGGAAGATACTCTGGCAAGGAACAG
  AAGACCTGTCTGTCCCTGGTGTGGAGCATGAAGCCCGTGTTCCCAAGAAAATCCTCAAGT
  GCAAGGCAGTGTCTCGAGAACTTAATTTTTCTTCGACAGAACAAATGGAAAAATTCCGCC
  TGGAACAAAAAGTTTACTTCAAAGGGCAATGCCTAGAAGAATGGTTCTTCGAGTTTGGCT
  TTGTGATCCCTAACTCCACAAATACCTGGCAGTCCTTGATAGAGGCAGCACCCGAGTCCC
  AGATGATGCCAGCAAGCGTCTTAACTGGGAACGTTATCATAGAAACAAAGTTTTTTGACG
  ACGATCTTCTTGTAAGCACATCCAGAGTGAGACTTTTCTATGTTTGAAAGAAGAATGTGT
  GTACATTTCAAGAATTTGGGTTTTTTGGAGGGAGGAGGAAACTGTTTACTTTTTTCCTNC
  ACACGTTTGATTTTNGACACATACACCCCTAATTCCCTCACAGCAGAACCTACCTGCAGC
  CACCANGGGACCAGCTCTGTGTAGGTACCAGATGGCTCTTTTCCAGCCGNCATCTTCAGC
  TGACAGACTAACTNCCAACCCAGACAGGCAGGGGAACAGTTTAAGTCCTTCCANCTACCA
  CGGAACAAAACATACACAACGGTACTGACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002601 unedited
  GGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
  TTCATATTTTTAATTACATTATGTAATAATATCACAGTCTATTTTACAAGTTAAATGTAT
  GAAAATGTATGAAATAAAAATGGTAGGAAAAAACTAAACATGGGTCAGGAACACGGAATT
  TTTCCAAAACCAGGGGCCAAAGGTAAATGTGTAGCCTGTAGGGCCCAAGGAGGAAACAAG
  GAGGGTGACAGGTACAGTTACCGGTTTGGGGTATGTGTTTGTTCCCTGTGTGTATGCTGG
  GAAGGACTTGAAACCTGTCCCCTGCCCTGGTCTGGGGTTGGGAGTTTAGTCTGGTCAGCT
  GGAAAATGGCGGCTTGGGAAAAAGAGCCATCTGGTTACCTACACAGAGCTGGTCCCCTGG
  TGGCTGCAGGTAGGTTCTGCTGTTGAGGGAATTAGGGGTGTATGTGTCAAAAATCAAACG
  TGTGGAGGAAAAAAGTAAACAGTTTCCTCCTCCCTCCAAAAAACCCAAATTCTTGAAATG
  TACACACATTCTTCTTTCAAACATAGAAAAGTCTCACTCTGGATGTGCTTACAAGAAGAT
  CGTCGTCAAAAAACTTTGTTTCTATGATAACGTTCCCAGGTAAGACGCTTGCTGGCATCA
  TCTGGGACCTCGGTGCTGCCTCTTCAAGGACTGCCAGTTTTTTGTGGATTAGGGATTCCA
  AACCCAACTTCAAGACCCTTTTTCTAGGCATTGCCCTTTAAATAAACTTTTTGTCCAGGC
  GGAATTTTTCTTTTGTTTTGTCAAAAAAAATAATTCTCAAAAACTGCCTGCCTTGAGGAT
  TTTTTGGAACCCGGCTTATGCTTCCCACAGGGAGACAGGGTTTTGTCTTTTCCAAATTTT
  TTCTCGTTAATCCCAAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002601
• Insert Size: 1070 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002601.2, NP_002592.1
• RefSeq Size: 1140 bp
• RefSeq ORF: 453 bp
• Locus ID: 5147
• Cytogenetics: 2q37.1
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).