PDK2 (NM_002611) Human Untagged Clone

CAT#: SC118540

PDK2 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_002611" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PDK2
Synonyms PDHK2; PDKII
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002611 edited
ATGCGCTGGGTGTGGGCGCTGCTGAAGAATGCGTCCCTGGCAGGGGCGCCCAAGTACATA
GAGCACTTCAGCAAGTTCTCCCCGTCCCCGCTGTCCATGAAGCAGTTTCTGGACTTCGGA
TCCAGCAATGCCTGTGAGAAAACCTCCTTCACCTTCCTCAGGCAGGAGCTGCCTGTGCGC
CTGGCCAACATCATGAAAGAGATCAACCTGCTTCCCGACCGAGTGCTGAGCACACCCTCC
GTGCAGCTGGTGCAGAGCTGGTATGTCCAGAGCCTCCTGGACATCATGGAGTTCCTGGAC
AAGGATCCCGAGGACCATCGCACCCTGAGCCAGTTCACTGACGCCCTGGTCACCATCCGG
AACCGGCACAACGACGTGGTGCCCACCATGGCACAAGGCGTGCTTGAGTACAAGGACACC
TACGGCGATGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTC
AGCCGCATCTCCATCCGCATGCTCATCAACCAGCACACCCTCATCTTTGATGGCAGCACC
AACCCAGCCCATCCCAAACACATCGGCAGCATCGACCCCAACTGCAACGTCTCTGAGGTG
GTCAAAGATGCCTACGACATGGCTAAGCTCCTGTGTGACAAGTATTACATGGCCTCACCT
GACCTGGAGATCCAGGAGATCAATGCAGCCAACTCCAAACAGCCGATTCACATGGTCTAC
GTCCCCTCCCACCTCTACCACATGCTCTTTGAGCTCTTCAAGAATGCCATGAGGGCGACT
GTGGAAAGCCATGAGTCCAGCCTCATTCTCCCACCCATCAAGGTCATGGTGGCCTTGGGT
GAGGAAGATCTGTCCATCAAGATGAGTGACCGAGGTGGGGGTGTTCCCTTGAGGAAGATT
GAGCGACTCTTCAGCTACATGTACTCCACAGCACCCACCCCCCAGCCTGGCACCGGGGGA
ACGCCGCTGGCTGGCTTTGGTTATGGGCTCCCCATTTCCCGCCTCTACGCCAAGTACTTC
CAGGGAGACCTGCAGCTCTTCTCCATGGAAGGCTTTGGGACCGATGCTGTCATCTATCTC
AAGGCCCTGTCCACGGACTCGGTGGAGCGCCTGCCTGTCTACAACAAGTCAGCCTGGCGC
CACTACCAGACCATCCAGGAGGCCGGCGACTGGTGTGTGCCCAGCACGGAGCCCAAGAAC
ACGTCCACGTACCGCGTCAGCTAA
>OriGene 5' read for NM_002611 unedited
GCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGCAGCCATGCGCT
GGGTGTGGGCGCTGCTGAAGATGCGTCCCTGGCAGGGGCGCCCAAGTACATAGAGCACTT
CAGCAAGTTCTCCCCGTCCCCGCTGTCCATGAAGCAGTTTCTGGACTTCGGATCCAGCAA
TGCCTGTGAGAAAACCTCCTTCACCTTCCTCAGGCAGGAGCTGCCTGTGCGCCTGGCCAA
CATCATGAAAGAGATCAACCTGCTTCCCGACCGAGTGCTGAGCACACCCTCCGTGCAGCT
GGTGCAGAGCTGGTATGTCCAGAGCCTCCTGGACATCATGGAGTTCCTGGACAAGGATCC
CGAGGACCATCGCACCCTGAGCCAGTTCACTGACGCCCTGGTCACCATCCGGAACCGGCA
CAACGACGTGGTGCCCACCATGGCACAAGGCGTGCTTGAGTACAAGGACACCTACGGCGA
TGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTCAGCCGCAT
CTCCATCCGCATGCTCATCAACCAGCACACCCTCATCTTTGATGGCAGCACCAACCCAGC
CCATCCCAAACACATCGGCAGCATCGACCCCAACTGCAACGTCTCTGAGGTGGTCAAAGA
TGCCTACGACATGGCTAAGCTCCTGTGTGACAAGTATTACATGGCCTCACCTGACCTGNA
GATCCAGGAGATCAATGCAGCCAACTCCAAACAGCCGATTCACATGGTCTACGTCCCCCT
CCACCTCTACCACATGCTCTTTGAGCTCTTCAGAATGCCCTGAGGGCGACTGTGGAAAGC
CATGAGTCCAGCCTCATTCTCCCACCATCAAGTCATGGTGCCCTTGGTGAGGAAAGACTG
TCCATCAGATATGACGAGGGNNGGGTGGTCCTTGAGGAGATGACCCATCTCACCTN
>OriGene 3' read for NM_002611 unedited
TATGAACCGCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTCTCATTCAGT
CTCCTCTTTACTGTGGGTGTGGGTGTCACTGTCACTGCCACAGCCACTGGGAGGGACACA
CAGCTTTAACCCCTGTGTGCTTAGGGGAAGGGTGGGGGCATTCAGGGTTATAAAACTAAC
TATATACACAGAAGGTCCTAGGGAGAAAGCCACCCTGAGCACACATGTCTGGGCACAGTG
GGGGCTGGGGGCTGGAGCTCAGGCAGGATGGACTAGGCTTGTGGAGGATCGGGTGGGCAT
GAGCATGTGAGGACATGCTGGGAGGGCTCAGGAGGTGGCACAGACATTGCCAAGGCCACT
GCAGGGCCTCGAGCAGCTTGGCTGGATGGCAAGCTCTCGGTCTAACAAGGTTCTTCCACT
TTAGAGTTGGGCCCTGTGAGAGCCACCCCCTCCGCCACCGACCTTCAAGCCCTGAGCGGA
GGGAGAGGCTGCAGGACCAGCCTCATTCCCCACTCACGGGTGTAACTCCTTTCCCCACTC
AGTCCCCAGGTCTGGAGGGCACCCACCCTCTTCCTAACACTTCAGCAGACCAAGGCCAGG
GTGCCTTTGTGGGCTGGGGGAATAAGGGCACGGGACATCCACTTGGAAGGCCTGTGCTGT
GGAGGGTGCTGAGGGGGACCCAGTACCCCTGGCAGAAAAGTGACAGCCCTGGTCTCAGAG
AGGAGGACTGCCATGGGATAATGTCTCTGGGACACCCCATCAGGGTGGAGACATCCCTAA
CCCTCAGGCATTCGCCACAGAGATAAGGNAGCCACCNGAGGGTCTNCCANAAGAGGACTG
CCCTTAGGGACCTCACAGACCTTCACCGCAATGACTCCATATAACAAAACCTGGCATCAG
GAAAACCCACCCCCTGCTCCCCAGTAGATGGTGAGGGCACCACGTGGGGGCACCAAGCGG
CATTCCGTCTTTTAGGGCAAAA
Restriction Sites NotI-NotI     
ACCN NM_002611
Insert Size 2320 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002611.3, NP_002602.2
RefSeq Size 2319 bp
RefSeq ORF 1224 bp
Locus ID 5164
Cytogenetics 17q21.33
Domains HATPase_c
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]'
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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