PDK2 (NM_002611) Human Untagged Clone

CAT#: SC118540

PDK2 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 2 (PDK2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDK2
• Synonyms: PDHK2; PDKII
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002611 edited
  ATGCGCTGGGTGTGGGCGCTGCTGAAGAATGCGTCCCTGGCAGGGGCGCCCAAGTACATA
  GAGCACTTCAGCAAGTTCTCCCCGTCCCCGCTGTCCATGAAGCAGTTTCTGGACTTCGGA
  TCCAGCAATGCCTGTGAGAAAACCTCCTTCACCTTCCTCAGGCAGGAGCTGCCTGTGCGC
  CTGGCCAACATCATGAAAGAGATCAACCTGCTTCCCGACCGAGTGCTGAGCACACCCTCC
  GTGCAGCTGGTGCAGAGCTGGTATGTCCAGAGCCTCCTGGACATCATGGAGTTCCTGGAC
  AAGGATCCCGAGGACCATCGCACCCTGAGCCAGTTCACTGACGCCCTGGTCACCATCCGG
  AACCGGCACAACGACGTGGTGCCCACCATGGCACAAGGCGTGCTTGAGTACAAGGACACC
  TACGGCGATGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTC
  AGCCGCATCTCCATCCGCATGCTCATCAACCAGCACACCCTCATCTTTGATGGCAGCACC
  AACCCAGCCCATCCCAAACACATCGGCAGCATCGACCCCAACTGCAACGTCTCTGAGGTG
  GTCAAAGATGCCTACGACATGGCTAAGCTCCTGTGTGACAAGTATTACATGGCCTCACCT
  GACCTGGAGATCCAGGAGATCAATGCAGCCAACTCCAAACAGCCGATTCACATGGTCTAC
  GTCCCCTCCCACCTCTACCACATGCTCTTTGAGCTCTTCAAGAATGCCATGAGGGCGACT
  GTGGAAAGCCATGAGTCCAGCCTCATTCTCCCACCCATCAAGGTCATGGTGGCCTTGGGT
  GAGGAAGATCTGTCCATCAAGATGAGTGACCGAGGTGGGGGTGTTCCCTTGAGGAAGATT
  GAGCGACTCTTCAGCTACATGTACTCCACAGCACCCACCCCCCAGCCTGGCACCGGGGGA
  ACGCCGCTGGCTGGCTTTGGTTATGGGCTCCCCATTTCCCGCCTCTACGCCAAGTACTTC
  CAGGGAGACCTGCAGCTCTTCTCCATGGAAGGCTTTGGGACCGATGCTGTCATCTATCTC
  AAGGCCCTGTCCACGGACTCGGTGGAGCGCCTGCCTGTCTACAACAAGTCAGCCTGGCGC
  CACTACCAGACCATCCAGGAGGCCGGCGACTGGTGTGTGCCCAGCACGGAGCCCAAGAAC
  ACGTCCACGTACCGCGTCAGCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002611 unedited
  GCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGCAGCCATGCGCT
  GGGTGTGGGCGCTGCTGAAGATGCGTCCCTGGCAGGGGCGCCCAAGTACATAGAGCACTT
  CAGCAAGTTCTCCCCGTCCCCGCTGTCCATGAAGCAGTTTCTGGACTTCGGATCCAGCAA
  TGCCTGTGAGAAAACCTCCTTCACCTTCCTCAGGCAGGAGCTGCCTGTGCGCCTGGCCAA
  CATCATGAAAGAGATCAACCTGCTTCCCGACCGAGTGCTGAGCACACCCTCCGTGCAGCT
  GGTGCAGAGCTGGTATGTCCAGAGCCTCCTGGACATCATGGAGTTCCTGGACAAGGATCC
  CGAGGACCATCGCACCCTGAGCCAGTTCACTGACGCCCTGGTCACCATCCGGAACCGGCA
  CAACGACGTGGTGCCCACCATGGCACAAGGCGTGCTTGAGTACAAGGACACCTACGGCGA
  TGACCCCGTCTCCAACCAGAACATCCAGTACTTCCTGGACCGCTTCTACCTCAGCCGCAT
  CTCCATCCGCATGCTCATCAACCAGCACACCCTCATCTTTGATGGCAGCACCAACCCAGC
  CCATCCCAAACACATCGGCAGCATCGACCCCAACTGCAACGTCTCTGAGGTGGTCAAAGA
  TGCCTACGACATGGCTAAGCTCCTGTGTGACAAGTATTACATGGCCTCACCTGACCTGNA
  GATCCAGGAGATCAATGCAGCCAACTCCAAACAGCCGATTCACATGGTCTACGTCCCCCT
  CCACCTCTACCACATGCTCTTTGAGCTCTTCAGAATGCCCTGAGGGCGACTGTGGAAAGC
  CATGAGTCCAGCCTCATTCTCCCACCATCAAGTCATGGTGCCCTTGGTGAGGAAAGACTG
  TCCATCAGATATGACGAGGGNNGGGTGGTCCTTGAGGAGATGACCCATCTCACCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002611 unedited
  TATGAACCGCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTCTCATTCAGT
  CTCCTCTTTACTGTGGGTGTGGGTGTCACTGTCACTGCCACAGCCACTGGGAGGGACACA
  CAGCTTTAACCCCTGTGTGCTTAGGGGAAGGGTGGGGGCATTCAGGGTTATAAAACTAAC
  TATATACACAGAAGGTCCTAGGGAGAAAGCCACCCTGAGCACACATGTCTGGGCACAGTG
  GGGGCTGGGGGCTGGAGCTCAGGCAGGATGGACTAGGCTTGTGGAGGATCGGGTGGGCAT
  GAGCATGTGAGGACATGCTGGGAGGGCTCAGGAGGTGGCACAGACATTGCCAAGGCCACT
  GCAGGGCCTCGAGCAGCTTGGCTGGATGGCAAGCTCTCGGTCTAACAAGGTTCTTCCACT
  TTAGAGTTGGGCCCTGTGAGAGCCACCCCCTCCGCCACCGACCTTCAAGCCCTGAGCGGA
  GGGAGAGGCTGCAGGACCAGCCTCATTCCCCACTCACGGGTGTAACTCCTTTCCCCACTC
  AGTCCCCAGGTCTGGAGGGCACCCACCCTCTTCCTAACACTTCAGCAGACCAAGGCCAGG
  GTGCCTTTGTGGGCTGGGGGAATAAGGGCACGGGACATCCACTTGGAAGGCCTGTGCTGT
  GGAGGGTGCTGAGGGGGACCCAGTACCCCTGGCAGAAAAGTGACAGCCCTGGTCTCAGAG
  AGGAGGACTGCCATGGGATAATGTCTCTGGGACACCCCATCAGGGTGGAGACATCCCTAA
  CCCTCAGGCATTCGCCACAGAGATAAGGNAGCCACCNGAGGGTCTNCCANAAGAGGACTG
  CCCTTAGGGACCTCACAGACCTTCACCGCAATGACTCCATATAACAAAACCTGGCATCAG
  GAAAACCCACCCCCTGCTCCCCAGTAGATGGTGAGGGCACCACGTGGGGGCACCAAGCGG
  CATTCCGTCTTTTAGGGCAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002611
• Insert Size: 2320 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002611.3, NP_002602.2
• RefSeq Size: 2319 bp
• RefSeq ORF: 1224 bp
• Locus ID: 5164
• Cytogenetics: 17q21.33
• Domains: HATPase_c
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.