PDK4 (NM_002612) Human Untagged Clone

CAT#: SC118542

PDK4 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 4 (PDK4), nuclear gene encoding mitochondrial protein


  "NM_002612" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PDK4
Synonyms FLJ40832
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002612 edited
ATGAAGGCGGCCCGCTTCGTGCTGCGCAGCGCTGGCTCGCTCAACGGCGCCGGCCTGGTG
CCCCGAGAGGTGGAGCATTTCTCGCGCTACAGCCCGTCCCCGCTGTCCATGAAGCAGCTA
CTGGACTTTGGTTCAGAAAATGCATGTGAAAGAACTTCTTTTGCATTTTTGCGACAAGAA
TTGCCTGTGAGACTCGCCAACATTCTGAAGGAAATTGATATCCTCCCGACCCAATTAGTA
AATACCTCTTCAGTGCAATTGGTTAAAAGCTGGTATATACAGAGCCTGATGGATTTGGTG
GAATTCCATGAGAAAAGCCCAGATGACCAGAAAGCATTATCAGACTTTGTAGATACACTC
ATCAAAGTTCGAAATAGACACCATAATGTAGTCCCTACAATGGCACAAGGAATCATAGAG
TATAAAGATGCCTGTACAGTTGACCCAGTCACCAATCAAAATCTTCAATATTTCTTGGAT
CGATTTTACATGAACCGTATTTCTACTCGGATGCTGATGAACCAGCACATTCTTATATTT
AGTGACTCACAGACAGGAAACCCAAGCCACATTGGAAGCATTGATCCTAACTGTGATGTG
GTAGCAGTGGTCCAAGATGCCTTTGAGTGTTCAAGGATGCTCTGTGATCAGTATTATTTA
TCATCTCCAGAATTAAAGCTTACACAAGTGAATGGAAAATTTCCAGACCAACCAATTCAC
ATCGTGTATGTTCCTTCTCACCTCCATCATATGCTCTTTGAACTATTTAAGAATGCAATG
CGGGCAACAGTTGAACACCAGGAAAATCAGCCTTCCCTTACACCAATAGAGGTTATTGTT
GTCTTGGGAAAAGAAGACCTTACCATTAAGATTTCAGACAGAGGAGGTGGTGTTCCCCTG
AGAATTATTGACCGCCTCTTTAGTTATACATACTCCACTGCACCAACGCCTGTGATGGAT
AATTCCCGGAATGCTCCTTTGGCTGGTTTTGGTTACGGCTTGCCAATTTCTCGTCTGTAT
GCCAAGTACTTTCAAGGAGATCTGAATCTCTACTCTTTATCAGGATATGGAACAGATGCT
ATCATCTACTTAAAGGCTTTGTCTTCTGAGTCTATAGAAAAACTTCCAGTTTTTAACAAG
TCAGCCTTCAAACATTATCAGATGAGCTCTGAGGCTGATGACTGGTGTATCCCAAGCAGG
GAACCAAAGAACCTGGCAAAAGAAGTGGCCATGTGA
>OriGene 5' read for NM_002612 unedited
NGGGGGTGCACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTTGA
ACTTGAGTCTCGAACCACTGCATCTCCGACCCTCTGCCCAGACTCTTCACTCCGCGGCAC
CCTCAAACCCCAGCCCAGGCCGGGGCGCACGAGCCAGCCAGCGCACCTGCAGTCCTCGCC
CGGACGCGCCGCGCCCCCTCGGAACCAGGCTCTGCTCCGAGCAGCCTTCGCCCCTCAAGC
CAGCCACAGTCCCCGCCAGGCCGGGTGGGCGTCAAGATGAAGGCGGCCCGCTTCGTGCTG
CGCAGCGCTGGCTCGCTCAACGGCGCCGGCCTGGTGCCCCGAGAGGTGGAGCATTTCTCG
CGCTACAGCCCGTCCCCGCTGTCCATGAAGCAGCTACTGGACTTTGGTTCAGAAAATGCA
TGTGAAAGAACTTCTTTTGCATTTTTGCGACAAGAATTGCCTGTGAGACTCGCCAACATT
CTGAAGGAAATTGATATCCTCCCGACCCAATTAGTAAATACCTCTTCAGTGCAATTGGTT
AAAAGCTGGTATATACAGAGCCTGATGGATTTGGTGGAATTCCATGAGAAAAGCCCAGAT
GACCAGAAAGCATTATCAGACTTTGTAGATACACTCATCAAAGTTCGAAATAGACACCAT
AATGTAGTCCCTACAATGGCACAAGGAATCATAGAGTATAAAGATGCCTGTACAGTTGAC
CCAGTCACCAATCAAAATCTTCAATATTTCTTGGATCGATTNTACATGAACCGTATTTCT
ACTCGGATGCTGATGAACCAGCACATTCTTATATTTAGTGACTCACAGACAGGNAAACCC
AGCCACATTGGAAGCATTGATCCTAAACTGTGATGTGGTAGCAGTGGTCCAAGATGCCTT
TTGAGTGTCAAG
Restriction Sites NotI-NotI     
ACCN NM_002612
Insert Size 3850 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002612.2, NP_002603.1
RefSeq Size 3634 bp
RefSeq ORF 1236 bp
Locus ID 5166
Cytogenetics 7q21.3
Domains HATPase_c
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]'

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