FURIN (NM_002569) Human Untagged Clone

CAT#: SC118550

FURIN (untagged)-Human furin (paired basic amino acid cleaving enzyme) (FURIN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FURIN
• Synonyms: FUR; PACE; PCSK3; SPC1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118550 sequence for NM_002569 edited (data generated by NextGen Sequencing)
  ATGGAGCTGAGGCCCTGGTTGCTATGGGTGGTAGCAGCAACAGGAACCTTGGTCCTGCTA
  GCAGCTGATGCTCAGGGCCAGAAGGTCTTCACCAACACGTGGGCTGTGCGCATCCCTGGA
  GGCCCAGCGGTGGCCAACAGTGTGGCACGGAAGCATGGGTTCCTCAACCTGGGCCAGATC
  TTCGGGGACTATTACCACTTCTGGCATCGAGGAGTGACGAAGCGGTCCCTGTCGCCTCAC
  CGCCCGCGGCACAGCCGGCTGCAGAGGGAGCCTCAATGGCTGGAACAGCAGGTGGCAAAG
  CGACGGACTAAACGGGACGTGTACCAGGAGCCCACAGACCCCAAGTTTCCTCAGCAGTGG
  TACCTGTCTGGTGTCACTCAGCGGGACCTGAATGTGAAGGCGGCCTGGGCGCAGGGCTAC
  ACAGGGCACGGCATTGTGGTCTCCATTCTGGACGATGGCATCGAGAAGAACCACCCGGAC
  TTGGCAGGCAATTATGATCCTGGGGCCAGTTTTGATGTCAATGACCAGGACCCTGACCCC
  CAGCCTCGGTACACACAGATGAATGACAACAGGCACGGCACACGGTGTGCGGGGGAAGTG
  GCTGCGGTGGCCAACAACGGTGTCTGTGGTGTAGGTGTGGCCTACAACGCCCGCATTGGA
  GGGGTGCGCATGCTGGATGGCGAGGTGACAGATGCAGTGGAGGCACGCTCGCTGGGCCTG
  AACCCCAACCACATCCACATCTACAGTGCCAGCTGGGGCCCCGAGGATGACGGCAAGACA
  GTGGATGGGCCAGCCCGCCTCGCCGAGGAGGCCTTCTTCCGTGGGGTTAGCCAGGGCCGA
  GGGGGGCTGGGCTCCATCTTTGTCTGGGCCTCGGGGAACGGGGGCCGGGAACATGACAGC
  TGCAACTGCGACGGCTACACCAACAGTATCTACACGCTGTCCATCAGCAGCGCCACGCAG
  TTTGGCAACGTGCCGTGGTACAGCGAGGCCTGCTCGTCCACACTGGCCACGACCTACAGC
  AGTGGCAACCAGAATGAGAAGCAGATCGTGACGACTGACTTGCGGCAGAAGTGCACGGAG
  TCTCACACGGGCACCTCAGCCTCTGCCCCCTTAGCAGCCGGCATCATTGCTCTCACCCTG
  GAGGCCAATAAGAACCTCACATGGCGGGACATGCAACACCTGGTGGTACAGACCTCGAAG
  CCAGCCCACCTCAATGCCAACGACTGGGCCACCAATGGTGTGGGCCGGAAAGTGAGCCAC
  TCATATGGCTACGGGCTTTTGGACGCAGGCGCCATGGTGGCCCTGGCCCAGAATTGGACC
  ACAGTGGCCCCCCAGCGGAAGTGCATCATCGACATCCTCACCGAGCCCAAAGACATCGGG
  AAACGGCTCGAGGTGCGGAAGACCGTGACCGCGTGCCTGGGCGAGCCCAACCACATCACT
  CGGCTGGAGCACGCTCAGGCGCGGCTCACCCTGTCCTATAATCGCCGTGGCGACCTGGCC
  ATCCACCTGGTCAGCCCCATGGGCACCCGCTCCACCCTGCTGGCAGCCAGGCCACATGAC
  TACTCCGCAGATGGGTTTAATGACTGGGCCTTCATGACAACTCATTCCTGGGATGAGGAT
  CCCTCTGGCGAGTGGGTCCTAGAGATTGAAAACACCAGCGAAGCCAACAACTATGGGACG
  CTGACCAAGTTCACCCTCGTACTCTATGGCACCGCCCCTGAGGGGCTGCCCGTACCTCCA
  GAAAGCAGTGGCTGCAAGACCCTCACGTCCAGTCAGGCCTGTGTGGTGTGCGAGGAAGGC
  TTCTCCCTGCACCAGAAGAGCTGTGTCCAGCACTGCCCTCCAGGCTTCGCCCCCCAAGTC
  CTCGATACGCACTATAGCACCGAGAATGACGTGGAGACCATCCGGGCCAGCGTCTGCGCC
  CCCTGCCACGCCTCATGTGCCACATGCCAGGGGCCGGCCCTGACAGACTGCCTCAGCTGC
  CCCAGCCACGCCTCCTTGGACCCTGTGGAGCAGACTTGCTCCCGGCAAAGCCAGAGCAGC
  CGAGAGTCCCCGCCACAGCAGCAGCCACCTCGGCTGCCCCCGGAGGTGGAGGCGGGGCAA
  CGGCTGCGGGCAGGGCTGCTGCCCTCACACCTGCCTGAGGTGGTGGCCGGCCTCAGCTGC
  GCCTTCATCGTGCTGGTCTTCGTCACTGTCTTCCTGGTCCTGCAGCTGCGCTCTGGCTTT
  AGTTTTCGGGGGGTGAAGGTGTACACCATGGACCGTGGCCTCATCTCCTACAAGGGGCTG
  CCCCCTGAAGCCTGGCAGGAGGAGTGCCCGTCTGACTCAGAAGAGGACGAGGGCCGGGGC
  GAGAGGACCGCCTTTATCAAAGACCAGAGCGCCCTCTGA
  
  Clone variation with respect to NM_002569.2
  277 g=>-;278 t=>-;279 a=>-;280 c=>-;281 a=>-;282 g=>-;1851 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002569 unedited
  CCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA
  GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT
  TCGGCACGAGGCCAAGGAGACGGGCGCTCCAGGGTCCCAGCCACCTGTCCCCCCCATGGA
  GCTGAGGCCCTGGTTGCTATGGGTGGTAGCAGCAACAGGAACCTTGGTCCTGCTAGCAGC
  TGATGCTCAGGGCCAGAAGGTCTTCACCAACACGTGGGCTGTGCGCATCCCTGGAGGCCC
  AGCGGTGGCCAACAGTGTGGCACGGAAGCATGGGTTCCTCAACCTGGGCCAGATCTTCGG
  GGACTATTACCACTTCTGGCATCGAGGAGTGACGAAGCGGTCCCTGTCGCCTCACCGCCC
  GCGGCACAGCCGGCTGCAGAGGGAGCCTCAATGGCTGGAACAGCAGGTGGCAAAGCGACG
  GACTAAACGGGACGTGTACCAGGAGCCCACAGACCCCAAGTTTCCTCAGCAGTGGTACCT
  GTCTGGTGTCACTCAGCGGGACCTGAATGTGAAGGCGGCCTGGGCGCAGGGCTACACAGG
  GCACGGCATTGTGGTCTCCATTCTGGACGATGGCATCGAGAAGAACCACCCGGACTTGGC
  AGGCAATTATGATCCTGGGGCCAGTTTTGATGTCAATGACCAGGACCCTGACCCCCAGCC
  TCGGTACACACAGATGAATGACAACAGGCACGGCACACGGTGTGCGGNGGAAGTGGCTGC
  GGTGGCCAACAAACGTGTCTGTGGTGTANGGTGTGGCCCTACACGCCCGCATTGGAGGGG
  TGCGCATGCTGGATGGCGAGGTGACAGATGCAGTGGAGGCACGCTCGCTGGGCCTGAACC
  CCAACCCATNCCATCTACGTGCCAGCTGGNGCCCGAGGATGACGCAGACATNGATGGGCC
  ACCCGCTCGCGAGGAGCCTCTCCGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002569 unedited
  GGTTTGTTCAGCCCGATCATGGGTTTAAAAAAAACCCATTATCTCATGTCCATTAAATTT
  AACCAGCAATGCTGGGCCTGTTTAAATTACAAGAAAAAAATCACTGTGCACCAACCCAGT
  ATCTTACAAAACCAGCCGGGCTGGCCACGAGGGCAGGGGGATGGGAGAGGAGGGGGCACC
  CCTGGGCAGATGGCTGGGTACCTGGAATGGCTTGGGTAGAAAAGAAAAGGGGACTCCCAG
  ATGGGAGGGGACTGATTGTCCTAATGGGAGGGGTGCAAAAGGCACGTCAGATGAGGAGGT
  TTGAGGGGGGCATGACAGGTCAGTGCTTTCTCCACCAGGGCACAGTGTTGGGGGGGCTCA
  GTGCCACCACCTGCCCACCTCTGGCACTTGGAGAGCCAGTGACCTATGAACCCCCGGCAT
  GGCGAGCCCCACTTGAAACACAGATGTCAGCCTGCCCCTCCCACTGGGACCCCCTTTCCT
  TTGAGCCCGCCTGGCTGGCCCGCCCTCTTCAGGACTTGGGGGCTCAACTTATTCCTCAAT
  TCTTAAGCAACATTGCTGCAAGCCCCCACACCTTAAGGCCAGGGCCAACCAGGGAGGAGC
  CAGGCGGGACGGTGGTGGGTGAAAACGTGGGTTGGTCCAGCTGGCTCCCCCCATTTAGAA
  TACTAAAACCGAAATCATCCGGCACTCTACGGGGGAAGTATCCCCAGGGTGGAAAAATTT
  ATCGCTTAAAACGGGCCCCTTGGGTATCCTCAACCAGGGGGAAAAACCACATATTTGTGG
  TGGGGAAGGTCACAAAGAAGGCCGGGCGTGGGGCCATACGCGCGTCTTCCCAACACACCC
  CACCCTGGGGGCTTCGGAGTTGTGTGGAGGTCCCAATAAGCCTAGGCGAGCCCGGGCGCC
  TAAAAGAAACAGGGTCCATGTGGGCCCCTGTACCGAAGAGAAGGCGGGGTCTCAGAAGAA
  GGCGACGCGGTGGTTCAAGCAGACACCACCNACATAAGTGTTCACCCACCACAGTGTATG
  CTGTTAGTGGTTGTCGCCACACACGGGGTGGCCCCCACGGGCGATGGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002569
• Insert Size: 4600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002569.2, NP_002560.1
• RefSeq Size: 4180 bp
• RefSeq ORF: 2385 bp
• Locus ID: 5045
• Cytogenetics: 15q26.1
• Domains: Peptidase_S8, P_proprotein, FU
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.