NPAS2 (NM_002518) Human Untagged Clone

CAT#: SC118571

NPAS2 (untagged)-Human neuronal PAS domain protein 2 (NPAS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPAS2
• Synonyms: bHLHe9; MOP4; PASD4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002518 edited
  GAGGGACCCGCGCGGCTGCGGCCCAGGAGCGGCGGCCGCGGAGCCCGGAGACCCGCAGCC
  GCGGCGGCGGCGGCGGCGGCGGCAGCAGCTAGAGCAGCGCCTCCCGCCGCCGCCCGGGAG
  GAGCTCGCCGCGCCCGCTCGCCGCCTCGTCTCCCAGCGGCGGCGGGAGGCGCGTCTCCCC
  GGCCCAGTCCGCGCCCGGCCCCGCGGGGCCGCTCCGGCCCGCTCCGAGGAAAAACTGCAT
  AGAAAATCTAATGGATGAAGATGAGAAAGACAGAGCCAAGAGAGCTTCTCGAAACAAGTC
  TGAGAAGAAGCGTCGGGACCAGTTCAATGTTCTCATCAAAGAGCTCAGTTCCATGCTCCC
  TGGCAACACGCGGAAAATGGACAAAACCACCGTGTTGGAAAAGGTCATCGGATTTTTGCA
  GAAACACAATGAAGTCTCAGCGCAAACGGAAATCTGTGACATTCAGCAAGACTGGAAGCC
  TTCATTCCTCAGTAATGAAGAATTCACCCAGCTGATGTTGGAGGCATTAGATGGCTTCAT
  TATCGCAGTGACAACAGACGGCAGCATCATCTATGTCTCTGACAGTATCACGCCTCTCCT
  TGGGCATTTACCGTCGGATGTCATGGATCAGAATTTGTTAAATTTCCTCCCAGAACAAGA
  ACATTCAGAAGTTTATAAAATCCTTTCTTCCCATATGCTTGTGACGGATTCCCCCTCCCC
  AGAATACTTAAAATCTGACAGCGATTTAGAGTTTTATTGCCATCTTCTCAGAGGCAGCTT
  GAACCCAAAGGAATTTCCAACTTATGAATACATAAAATTTGTAGGAAATTTTCGCTCTTA
  CAACAATGTGCCTAGCCCCTCCTGTAATGGTTTTGACAACACCCTTTCAAGACCTTGCCG
  GGTGCCACTAGGAAAGGAGGTTTGCTTCATTGCCACCGTTCGTCTGGCAACACCACAATT
  CTTAAAGGAAATGTGCATAGTTGACGAACCTTTAGAGGAATTCACTTCAAGGCATAGCTT
  GGAATGGAAATTTTTATTTCTGGATCACAGAGCACCTCCAATCATAGGATACCTGCCTTT
  TGAAGTGCTGGGAACCTCAGGCTATGACTACTACCACATTGATGACCTGGAGCTCCTGGC
  CAGGTGTCACCAGCACCTGATGCAGTTTGGCAAAGGGAAGTCGTGTTGCTACCGGTTTCT
  GACCAAAGGTCAGCAGTGGATCTGGCTGCAGACTCACTACTACATCACCTACCATCAGTG
  GAACTCCAAGCCCGAGTTCATCGTGTGCACACACTCGGTGGTCAGTTACGCAGATGTCCG
  GGTGGAAAGGAGGCAGGAGCTGGCTCTGGAAGACCCGCCATCCGAGGCCCTCCACTCCTC
  AGCACTAAAGGACAAGGGCTCAAGCCTGGAACCTCGGCAGCACTTTAACGCACTCGACGT
  GGGTGCCTCGGGCCTTAATACCAGTCATTCGCCATCGGCGTCCTCAAGAAGTTCCCACAA
  ATCCTCGCACACAGCCATGTCAGAACCCACCTCCACTCCCACCAAGCTGATGGCAGAGGC
  CAGCACCCCGGCTTTGCCAAGATCAGCCACCCTGCCCCAAGAGTTACCTGTCCCCGGGCT
  CAGCCAGGCAGCCACCATGCCGGCCCCTCTGCCTTCCCCATCGTCCTGCGACCTCACACA
  GCAGCTCCTGCCTCAGACCGTTCTGCAGAGCACGCCCGCTCCCATGGCACAGTTTTCGGC
  ACAGTTCAGCATGTTCCAGACCATCAAAGACCAGCTAGAGCAGCGGACGCGGATCCTGCA
  GGCCAATATCCGGTGGCAACAGGAAGAGCTCCACAAGATCCAGGAGCAGCTCTGCCTGGT
  CCAGGACTCCAACGTCCAGATGTTCCTGCAGCAGCCAGCTGTATCCCTGAGCTTCAGCAG
  CACCCAGCGACCTGAGGCTCAGCAGCAGCTACAGCAAAGGTCAGCTGCAGTGACTCAGCC
  CCAGCTCGGGGCGGGCCCCCAACTTCCAGGGCAGATCTCCTCTGCCCAGGTCACAAGCCA
  GCACCTGCTCAGAGAATCAAGTGTGATATCAACCCAGGGTCCAAAGCCAATGAGAAGCTC
  ACAGCTAATGCAGAGCAGCGGCCGCTCTGGAAGCAGCCTAGTGTCCCCGTTCAGCAGCGC
  CACAGCTGCGCTCCCGCCAAGTCTGAATCTGACCACACCTGCTTCCACCTCCCAGGATGC
  CAGCCAGTGCCAGCCCAGCCCAGACTTCAGCCATGATCGGCAGCTCAGGCTGTTGCTGAG
  CCAGCCCATCCAGCCCATGATGCCCGGGTCCTGTGACGCAAGGCAGCCCTCGGAAGTCAG
  CAGGACGGGACGGCAAGTCAAGTACGCCCAGAGCCAGACTGTGTTTCAAAATCCAGACGC
  ACACCCCGCCAACAGCAGCAGCGCCCCGATGCCCGTCCTGCTGATGGGGCAGGCGGTGCT
  CCACCCCAGCTTCCCTGCCTCCCAACCATCGCCCCTGCAGCCTGCACAGGCCCGGCAGCA
  GCCACCGCAGCACTACCTGCAGGTACAGGCACCAACCTCTTTGCACAGTGAGCAGCAGGA
  CTCGCTACTTCTCTCCACCTACTCACAACAGCCAGGGACCCTGGGCTACCCCCAACCACC
  CCCAGCACAGCCCCAGCCCCTACGTCCTCCCCGAAGGGTCAGCAGTCTGTCTGAGTCGTC
  AGGCCTCCAGCAGCCGCCCCGATAAT:GCCCCGGC:ACTGAAGTCGGGACACAATCAGCT
  TTAACCAATGGATGAGGGGGGTGGCCACAGGAGATGGGGAGAGGAGTCTGAACTAAACCC
  CTGGCTTTTGTGCACACTGCATACGTTTCAGAACTCCTGGATGGTAACCATCTCTGGAGT
  GCAGCGCTTGCTGCAGTGGAAATGATCAGGAATACTGACCGTGTTTCTCTTGCCTCCGAG
  GTTCTTGGGCACACTCTATAGCCATACTGGACAGGAACCAGGTGCCCCGTGTAGGCATCG
  TCGGTCGGTTTGCCGTCAGAGATGGCGCATCTCGCTGCATCCCCCGAGAGTACACCGGTT
  GCTCTAGCCACCTGCGGCCCGCCCATCTGCGCTAGCTGGCCTTCACGCTCTTGATCGTCT
  TTCCTTTGTATTGGAGAAGGACTGGGTCAGAGATCTGTTGGAGAGAGAGAATAAAGAGAT
  TATTTTTCATTATTTTTAAATGGTTGTTTTTGTTTTAATTTGCACAGCTACACAGAGGAA
  ATAACTTAGGTACTTTCTGTTTTTTTAAAAAAAATAATAAGGTCTCATGGCTTCATTCAG
  AGACCACAGTAACAACAGCAGCCCACCAATCAGAGAAGCTGGTTGTTATTAACCAAGCTA
  CAGATTCACACTTTCTGGCCTAAACCCTAATGGGATGAGGCTTTTCACCCCAGGCCATGC
  TGGTGGTGATTTTTTAGCCCCTAAATAAAACACTGGACTATTTCCTGTTTACTTCATTGA
  TTGCAACTACAAAGGTGGACTCAAAGCAAAGCACAATCATGCCAGCCAACATTCCAGAAT
  TCTGCTGAGAACTCCAAGTCTGTGAGGGGAGAGGTTTTACAAGCCAGACAGGCCTGGGGG
  ACTGCAGTCCCCAAGGAGACCCTGCCACATGCTGGCCCTTTGAGTGAGAATGCTGCATCT
  TTCTACATATCTTCATGAGAATACTGAGAATTGGATTTTCCTTTTCAAAATGCACTTTGC
  TTTTTTTGTATGTTTTGTTATGTTGAGATGTTTCTAAAGAAAAGATTTTATGTAATTATA
  AGATGAAGCGTAGTGAATTGTACAGCTGTTGTAATAATGACCTATTTCTATATAAAATAA
  AATTGTATGGCTTATGTGTAAATTATTTTGTATCTGAGATACCAGTTCCTTTTC:CCAAA
  TATAAAAGTATAAAAGTTAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002518
• Insert Size: 3900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002518.3, NP_002509.2
• RefSeq Size: 4004 bp
• RefSeq ORF: 2475 bp
• Locus ID: 4862
• Cytogenetics: 2q11.2
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: 'The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq, Jul 2008]'