NRAS (NM_002524) Human Untagged Clone

CAT#: SC118574

NRAS (untagged)-Human neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRAS
• Synonyms: ALPS4; CMNS; N-ras; NCMS; NRAS1; NS6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002524 edited
  GAATTCGGCACGAGGCCTTGGTGGGGGCTGTTCATGGCGGTTCCGGGGTCTCCAACATTT
  TTCCCGGCTGTGGTCCTAAATCTGTCCAAAGCAGAGGCAGTGGAGCTTGAGGTTCTTGCT
  GGTGTGAAATGACTGAGTACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCG
  CACTGACAATCCAGCTAATCCAGAACCACTTTGTAGATGAATATGATCCCACCATAGAGG
  ATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACCTGTTTGTTGGACATACTGGATA
  CAGCTGGACAAGAAGAGTACAGTGCCATGAGAGACCAATACATGAGGACAGGCGAAGGCT
  TCCTCTGTGTATTTGCCATCAATAATAGCAAGTCATTTGCGGATATTAACCTCTACAGGG
  AGCAGATTAAGCGAGTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGT
  GTGATTTGCCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACG
  GGATTCCATTCATTGAAACCTCAGCCAAGACCAGACAGGGTGTTGAAGATGCTTTTTACA
  CACTGGTAAGAGAAATACGCCAGTACCGAATGAAAAAACTCAACAGCAGTGATGATGGGA
  CTCAGGGTTGTATGGGATTGCCATGTGTGGTGATGTAACAAGATACTTTTAAAGTTTTGT
  CAGAAAAGAGCCACTTTCAAGCTGCACTGACACCCTGGTCCTGACTTCCCTGGAGGAGAA
  GTATTCCTGTTGCTGTCTTCAGTCTCACAGAGAAGCTCCTGCTACTTCCCCAGCTCTCAG
  TAGTTTAGTACAATAATCTCTATTTGAGAAGTTCTCAGAATAACTACCTCCTCACTTGGC
  TGTCTGACCAGAGAATGCACCTCTTGTTACTCCCTGTTATTTTTCTGCCCTGGGTTCTTC
  CACAGCACAAACACACCTCTGCCACCCCAGGTTTTTCATCTGAAAAGCAGTTCATGTCTG
  AAACAGAGAACCAAACCGCAAACGTGAAATTCTATTGAAAACAGTGTCTTGAGCTCTAAA
  GTAGCAACTGCTGGTGATTTTTTTTTTCTTTTTACTGTTGAACTTAGAACTATGCTAATT
  TTTGGAGAAATGTCATAAATTACTGTTTTGCCAAGAATATAGTTATTATTGCTGTTTGGT
  TTGTTTATAATGTTATCGGCTCTATTCTCTAAACTGGCATCTGCTCTAGATTCATAAATA
  CAAAAATGAATACTGAATTTTGAGTCTATCCTAGTCTTCACAACTTTGACGTAATTAAAT
  CCAACTTTCACAGTGAAGTGCCTTTTTCCTAGAAGTGGTTTGTAGACTTCCTTTATAATA
  TTTCAGTGGAATAGATGTCTCAAAAATCCTTATGCATGAAATGAATGTCTGAGATACGTC
  TGTGACTTATCTACCATTGAAGGAAAGCTATATCTATTTGAGAGCAGATGCCATTTTGTA
  CATGTATGAAATTGGTTTTCCAGAGGCCTGTTTTGGGGCTTTCCCAGGAGAAAGATGAAA
  CTGAAAGCATATGAATAATTTCACTTAATAATTTTTACCTAATCTCCACTTTTTTCATAG
  GTTACTACCTATACAATGTATGTAATTTGTTTCCCCTAGCTTACTGATAAACCTAATATT
  CAATGAACTTCCATTTGTATTCAAATTTGTGTCATACCAGAAAGCTCTACATTTGCAGAT
  GTTCAAATATTGTAAAACTTTGGTGCATTGTTATTTAATAGCTGTGATCAGTGATTTTCA
  AACCTCAAATATAGTATATTAACAAATTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002524 unedited
  TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTGGTGGGGGCTG
  TTCATGGCGGTTCCGGGGTCTCCAACATTTTTCCCGGCTGTGGTCCTAAATCTGTCCAAA
  GCAGAGGCAGTGGAGCTTGAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGG
  TGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACAATCCAGCTAATCCAGAACCACT
  TTGTAGATGAATATGATCCCACCATAGAGGATTCTTACAGAAAACAAGTGGTTATAGATG
  GTGAAACCTGTTTGTTGGACATACTGGATACAGCTGGACAAGAAGAGTACAGTGCCATGA
  GAGACCAATACATGAGGACAGGCGAAGGCTTCCTCTGTGTATTTGCCATCAATAATAGCA
  AGTCATTTGCGGATATTAACCTCTACAGGGAGCAGATTAAGCGAGTAAAAGACTCGGATG
  ATGTACCTATGGTGCTAGTGGGAAACAAGTGTGATTTGCCAACAAGGACAGTTGATACAA
  AACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATTCATTGAAACCTCAGCCAAGA
  CCAGACAGGGTGTTGAAGATGCTTTTTACACACTGGTAAGAGAAATACGCCAGTACCGAA
  TGAAAAAACTCAACAGCAGTGATGATGGGACTCAGGNTTGTATGGGATTGCCATGTGTGG
  TGATGTAACAANGATACTTTAAAGTTTTGTCAGAAAAGAGCCACTTTTCAGCTGCACTGA
  CACCCTGGTCCTGACTTCCCTGGNAGAGAAGTATNCCTGTTGCTGTCTTCAGTCTCACAG
  AGAAGCTNCTGCTACTTNCCCACTCTNAGTAGTTNAGNACAATATCTCTATTTGAGAAGT
  CTCAAATAACTACCTCTCACTGGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002524 unedited
  GTACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTGTTAATATA
  CTATATTTGAGGTTTGAAAATCACTGATCACAGCTATTAAATAACAATGCACCAAAGTTT
  TACAATATTTGAACATCTGCAAATGTAGAGCTTTCTGGTATGACACAAATTTGAATACAA
  ATGGAAGTTCATTGAATATTAGGTTTATCAGTAAGCTAGGGGAAACAAATTACATACATT
  GTATAGGTAGTAACCTATGAAAAAAGTGGAGATTAGGTAAAAATTATTAAGTGAAATTAT
  TCATATGCTTTCAGTTTCATCTTTCTCCTGGGAAAGCCCCAAAACAGGCCTCTGGAAAAC
  CAATTTCATACATGTACAAAATGGCATCTGCTCTCAAATAGATATAGCTTTCCTTCAATG
  GTAGATAAGTCACAGACGTATCTCAGACATTCATTTCATGCATAAGGATTTTTGAGACAT
  CTATTCCACTGAAATATTATAAAGGAAGTCTACAAACCACTTCTAGGAAAAAGGCACTTC
  ACTGTGAAAGTTGGATTTAATTACGTCAAAGTTGTGAAGACTAGGATAGACTCAAAATTC
  AGTATTCATTTTTGTATTTATGAATCTAGAGCAGATGCCAGTTTAGAGAATAGAGCCGAT
  AACATTATAAACAAACCAAACAGCACTAATAACTATATTTCTTGGCAAAACAGTAATTTA
  TGACATTTCTCCAAAAATAGCATAGTTCTAAGTTCAACAGTAAAAAGAAAAAAAAATCAC
  CACCAGTTGCTACTTTAGAGCTCAAGAACTGTTTTCATAGAATTCACGTTTGCGGTTTGG
  TTCTCTGTTCAGACATGACTGCTTTCAGATGAAAACCTGGGTGGCANAAGTTGTGTTGTG
  CTGTGGAAAACCCAGGCCAAATAATACAGGGGTTACAGGAGGGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002524
• Insert Size: 1860 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002524.2, NP_002515.1
• RefSeq Size: 1963 bp
• RefSeq ORF: 570 bp
• Locus ID: 4893
• Cytogenetics: 1p13.2
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
• Gene Summary: 'This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]'