Occludin (OCLN) (NM_002538) Human Untagged Clone

CAT#: SC118584

OCLN (untagged)-Human occludin (OCLN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OCLN
• Synonyms: BLCPMG; PPP1R115; PTORCH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118584 sequence for NM_002538 edited (data generated by NextGen Sequencing)
  ATGTCATCCAGGCCTCTTGAAAGTCCACCTCCTTACAGGCCTGATGAATTCAAACCGAAT
  CATTATGCACCAAGCAATGACATATATGGTGGAGAGATGCATGTTCGACCAATGCTCTCT
  CAGCCAGCCTACTCTTTTTACCCAGAAGATGAAATTCTTCACTTCTACAAATGGACCTCT
  CCTCCAGGAGTGATTCGGATCCTGTCTATGCTCATTATTGTGATGTGCATTGCCATCTTT
  GCCTGTGTGGCCTCCACGCTTGCCTGGGACAGAGGCTATGGAACTTCCCTTTTAGGAGGT
  AGTGTAGGCTACCCTTATGGAGGAAGTGGCTTTGGTAGCTACGGAAGTGGCTATGGCTAT
  GGCTATGGTTATGGCTATGGCTACGGAGGCTATACAGACCCAAGAGCAGCAAAGGGCTTC
  ATGTTGGCCATGGCTGCCTTTTGTTTCATTGCCGCGTTGGTGATCTTTGTTACCAGTGTT
  ATAAGATCTGAAATGTCCAGAACAAGAAGATACTACTTAAGTGTGATAATAGTGAGTGCT
  ATCCTGGGCATCATGGTGTTTATTGCCACAATTGTCTATATAATGGGAGTGAACCCAACT
  GCTCAGTCTTCTGGATCTCTATATGGTTCACAAATATATGCCCTCTGCAACCAATTTTAT
  ACACCTGCAGCTACTGGACTCTACGTGGATCAGTATTTGTATCACTACTGTGTTGTGGAT
  CCCCAGGAGGCCATTGCCATTGTACTGGGGTTCATGATTATTGTGGCTTTTGCTTTAATA
  ATTTTCTTTGCTGTGAAAACTCGAAGAAAGATGGACAGGTATGACAAGTCCAATATTTTG
  TGGGACAAGGAACACATTTATGATGAGCAGCCCCCCAATGTCGAGGAGTGGGTTAAAAAT
  GTGTCTGCAGGCACACAGGACGTGCCTTCACCCCCATCTGACTATGTGGAAAGAGTTGAC
  AGTCCCATGGCATACTCTTCCAATGGCAAAGTGAATGACAAGCGGTTTTATCCAGAGTCT
  TCCTATAAATCCACGCCGGTTCCTGAAGTGGTTCAGGAGCTTCCATTAACTTCGCCTGTG
  GATGACTTCAGGCAGCCTCGTTACAGCAGCGGTGGTAACTTTGAGACACCTTCAAAAAGA
  GCACCTGCAAAGGGAAGAGCAGGAAGGTCAAAGAGAACAGAGCAAGATCACTATGAGACA
  GACTACACAACTGGCGGCGAGTCCTGTGATGAGCTGGAGGAGGACTGGATCAGGGAATAT
  CCACCTATCACTTCAGATCAACAAAGACAACTGTACAAGAGGAATTTTGACACTGGCCTA
  CAGGAATACAAGAGCTTACAATCAGAACTTGATGAGATCAATAAAGAACTCTCCCGTTTG
  GATAAAGAATTGGATGACTATAGAGAAGAAAGTGAAGAGTACATGGCTGCTGCTGATGAA
  TACAATAGACTGAAGCAAGTGAAGGGATCTGCAGATTACAAAAGTAAGAAGAATCATTGC
  AAGCAGTTAAAGAGCAAATTGTCACACATCAAGAAGATGGTTGGAGACTATGATAGACAG
  AAAACATAG
  
  Clone variation with respect to NM_002538.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002538 unedited
  NNGGGGCCNNNNNNNNNNNNNAACCCCNNGGTTCAGAATTGTATACGACTCACTATAGGC
  GGCCGCGAATTCGGCACCAGAGGAAACCGAGAACCAGGTCCAGAGCGCCGAGGAGCCGGT
  CTAGGACGCAGCAGATTGGTTTATCTTGGAAGCTAAAGGGCATTGCTCATCCTGAAGATC
  AGCTGACCATTGACAATCAGCCATGTCATCCAGGCCTCTTGAAAGTCCACCTCCTTACAG
  GCCTGATGAATTCAAACCGAATCATTATGCACCAAGCAATGACATATATGGTGGAGAGAT
  GCATGTTCGACCAATGCTCTCTCAGCCAGCCTACTCTTTTTACCCAGAAGATGAAATTCT
  TCACTTCTACAAATGGACCTCTCCTCCAGGAGTGATTCGGATCCTGTCTATGCTCATTAT
  TGTGATGTGCATTGCCATCTTTGCCTGTGTGGCCTCCACGCTTGCCTGGGACAGAGGCTA
  TGGAACTTCCCTTTTAGGAGGTAGTGTAGGCTACCCTTATGGAGGAAGTGGCTTTGGTAG
  CTACGGAAGTGGCTATGGCTATGGCTATGGTTATGGCTATGGCTACGGAGGCTATACAGA
  CCCAAGAGCAGCAAAGGGCTTCATGTTGGCCATGGCTGCCTTTTGTTTCATTGCCGCGTT
  GGTGATCTTTGTTACCAGTGTTATAAGATCTGANATGTCCAGAACAAGAAGATACTACTT
  AAGTGTGATAATAGTGAGTGCTATCCTGGGCATCATGGTGTTNNATTGCCACANTGTCTA
  TATAATGGGAGTGAACCCNACTGCTCAGTCTTCTGGATCTCTATATGGNTCACAAATATA
  TGCCCTCTGNCACCAATTTATACACCTGCAGCTACTGGACTCTACGTGGATCAGTATTGT
  ATCACTACTGNNGTNGGGNATCCCCAGNAGGCCATNGNNCATNGTACTGGGGNNTCATGA
  TTATGNGGGCTNNTGCTTATAAATNTCTTTGCTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002538 unedited
  GTTGAGTTTTATTTTTTTTTTTTTTTACTTTTACATGATCTTTATTATTTAAGAAAAACC
  TCTTTTAACCATTTATATAACAGAAAAAAAATAGGGAGGCTGGTAGATCATCACATATAT
  AGTAGCTAAAATATGAAAGGCCAGGGAATTTATTATTAATGAAGTCATAAAACAGACTTA
  ACCAAAAGTGTGTGCTAGGAAACAAGCAGTTTCACTTCAGAGACTTCATTGCAGGAACCC
  AGTTTCCTTATGTGGAAAAAAGTGATTATAAATAACAGTTATCTGAAAGGTGGTTGAGAG
  GATTAAATGAGATCACCTATGCAAACAAATACATGTAGGTATGAAGACATCGTCTGGGGT
  GTGAAAGTTTAGTTCACACCAGAACCTTCCTTTAAGGCTTAAAAACCTCAAACAGTATTT
  CATTCTTAATACAAGAACCAATGTTTAAAGCATAAAATCCTTAATTGGAGTGTTCAGCCC
  AGTTGATTATCAAAAGCGATTTATAAAATGCTTCAATACTGATGATGTTAAAGCAACCAA
  AACTTTGTGATGCTCACAGAGGTTTGGCTTCCGGGGTTATGGTCCAAAGTCATTTGCCTT
  CTGAGAAGATTGCAGAGATGTCAGATACTTAATTTCTCAAACAACTTGGCATCAGCCTTC
  TATGTTTTCTGTCTATCATAGTCTCCCACCATCTTCTTGATGTGTGACAATTTGCTCTTT
  AACTGGCTGCAATGANNTCTNNCTACTTTGNAATCTGCAGATCCCCTCACTTGCTTCAGT
  CTATTGTATTCATCAGCAGCAGCCATGTACTCTTCACTTTCTTCTCTATAGTCATCCAAT
  TTCTTTATCCAACGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002538
• ORF Size: 1569 bp
• Insert Size: 2500
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_002538.2, NP_002529.1
• RefSeq Size: 2648
• RefSeq ORF: 1569
• Locus ID: 100506658
• Domains: MARVEL
• Gene Summary: This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.