P2RX7 (AB209709) Human Untagged Clone

CAT#: SC118597

(untagged)-Human mRNA for P2X purinoceptor 7 variant protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P2RX7
• Synonyms: P2X7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AB209709, the custom clone sequence may differ by one or more nucleotides
  
  
  CTGTGGCCCTGTCAGGAAGAGTAGAGCTCTGGTCCAGCTCCGCGCAGGGAGGGAGGCTGTCACCATGCCG
  GCCTGCTGCAGCTGCAGTGATGTTTTCCAGTATGAGACGAACAAAGTCACTCGGATCCAGAGCATGAATT
  ATGGCACCATTAAGTGGTTCTTCCACGTGATCATCTTTTCCTACGTTTGCTTTGCTCTGGTGAGTGACAA
  GCTGTACCAGCGGAAAGAGCCTGTCATCAGTTCTGTGCACACCAAGGTGAAGGGGATAGCAGAGGTGAAA
  GAGGAGATCGTGGAGAATGGAGTGAAGAAGTTGGTGCACAGTGTCTTTGACACCGCAGACTACACCTTCC
  CTTTGCAGGGGAACTCTTTCTTCGTGATGACAAACTTTCTCAAAACAGAAGGCCAAGAGCAGCGGTTGTG
  TCCCGAGTATCCCACCCGCAGGACGCTCTGTTCCTCTGACCGAGGTTGTAAAAAGGGATGGATGGACCCG
  CAGAGCAAAGGAATTCAGACCGGAAGGTGTGTAGTGCATGAAGGGAACCAGAAGACCTGTGAAGTCTCTG
  CCTGGTGCCCCATCGAGGCAGTGGAAGAGGCCCCCCGGCCTGCTCTCTTGAACAGTGCCGAAAACTTCAC
  TGTGCTCATCAAGAACAATATCGACTTCCCCGGCCACAACTACACCACGAGAAACATCCTGCCAGGTTTA
  AACATCACTTGTACCTTCCACAAGACTCAGAATCCACAGTGTCCCATTTTCCGACTAGGAGACATCTTCC
  GAGAAACAGGCGATAATTTTTCAGATGTGGCAATTCAGGGCGGAATAATGGGCATTGAGATCTACTGGGA
  CTGCAACCTAGACCGTTGGTTCCATCACTGCCGTCCCAAATACAGTTTCCGTCGCCTTGACGACAAGACC
  ACCAACGTGTCCTTGTACCCTGGCTACAACTTCAGATACGCCAAGTACTACAAGGAAAACAATGTTGAGA
  AACGGACTCTGATAAAAGTCTTCGGGATCCGTTTTGACATCCTGGTTTTTGGCACCGGAGGAAAATTTGA
  CATTATCCAGCTGGTTGTGTACATCGGCTCAACCCTCTCCTACTTCGGTCTGGTAAGAGATTCTCTTTTC
  CATGCTTTAGGAAAATGGTTTGGAGAAGGAAGTGACTAACGCAGCGCTTGTCTGCATTCTCCCCAGGCCA
  CTGTGTTCATCGACTTCCTCATCGACACTTACTCCAGTAACTGCTGTCGCTCCCATATTTATCCCTGGTG
  CAAGTGCTGTCAGCCCTGTGTGGTCAACGAATACTACTACAGGAAGAAGTGCGAGTCCATTGTGGAGCCA
  AAGCCGACATTAAAGTATGTGTCCTTTGTGGATGAATCCCACATTAGGATGGTGAACCAGCAGCTACTAG
  GGAGAAGTCTGCAAGATGTCAAGGGCCAAGAAGTCCCAAGACCTGCGATGGACTTCACAGATTTGTCCAG
  GCTGCCCCTGGCCCTCCATGACACACCCCCGATTCCTGGACAACCAGAGGAGATACAGCTGCTTAGAAAG
  GAGGCGACTCCTAGATCCAGGGATAGCCCCGTCTGGTGCCAGTGTGGAAGCTGCCTCCCATCTCAACTCC
  CTGAGAGCCACAGGTGCCTGGAGGAGCTGTGCTGCCGGAAAAAGCCGGGGGCCTGCATCACCACCTCAGA
  GCTGTTCAGGAAGCTGGTCCTGTCCAGACACGTCCTGCAGTTCCTCCTGCTCTACCAGGAGCCCTTGCTG
  GCGCTGGATGTGGATTCCACCAACAGCCGGCTGCGGCACTGTGCCTACAGGTGCTACGCCACCTGGCGCT
  TCGGCTCCCAGGACATGGCTGACTTTGCCATCCTGCCCAGCTGCTGCCGCTGGAGGATCCGGAAAGAGTT
  TCCAAAGAGTGAAGGGCAGTACAGTGGCTTCAAGAGTCCTTACTGAAGCCAGGCACCGTGGCTCACGTCT
  GTAATCCCAGCGCTTTGGGAGGCCGAGGCAGGCAGATCACCTGAGGTCGGGAGTTGGAGACCCGCCTGGC
  TAACAAGGCGAAATCCTGTCTGTACTAAAAATACAAAAATCAGCCAGACATGGTGGCATGCACCTGCAAT
  CCCAGCTACTCGGGAGGCTGAGGCACAAGAATCACTTGAACCCGGGAGGCAGAGGTTGTAGTGAGCCCAG
  ATTGTGCCACTGCTCTCCAGCCTGGGAGGCACAGCAAACTGTCCC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AB209709 unedited
  NGGTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGGCCCTG
  TCAGGAAGAGTAGAGCTCTGGTCCAGCTCCGCGCAGGGAGGGAGGCTGTCACCATGCCGG
  CCTGCTGCAGCTGCAGTGATGTTTTCCAGTATGAGACGAACAAAGTCACTCGGATCCAGA
  GCATGAATTATGGCACCATTAAGTGGTTCTTCCACGTGATCATCTTTTCCTACGTTTGCT
  TTGCTCTGGTGAGTGACAAGCTGTACCAGCGGAAAGAGCCTGTCATCAGTTCTGTGCACA
  CCAAGGTGAAGGGGATAGCAGAGGTGAAAGAGGAGATCGTGGAGAATGGAGTGAAGAAGT
  TGGTGCACAGTGTCTTTGACACCGCAGACTACACCTTCCCTTTGCAGGGGAACTCTTTCT
  TCGTGATGACAAACTTTCTCAAAACAGAAGGCCAAGAGCAGCGGTTGTGTCCCGAGTATC
  CCACCCGCAGGACGCTCTGTTCCTCTGACCGAGGTTGTAAAAAGGGATGGATGGACCCGC
  AGAGCAAAGGAATTCAGACCGGAAGGTGTGTAGTGCATGAAGGGAACCAGAAGACCTGTG
  AAGTCTCTGCCTGGTGCCCCATCGAGGCAGTGGAAGAGGCCCCCCGGCCTGCTCTCTTGA
  ACAGTGCCGAAACTTTCACTGTGCTCATCAAGAACAATATCGACTTCCCCGGGCCACACT
  ACACCACGAGAAACATCCTGCCAGGTTTAAACATCACTTGTACCTTCCACAAGACTCAGA
  ATCCACAGTGTCCCATTTTTCGACTANGAGACATCTTCCGAGAAACAGGCGATAATTTTT
  CAGATGTGGCAATCAGGNNCGGATATGGGCCATTGAGATCTACTGGGACTGCACCTANAC
  CG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AB209709 unedited
  AGGTCGTTGCTGTGCCCCCCCAGTCTGGNATACAGTGGCACAATCTGGGCTCACTACAAC
  CTCTGCCTCCCGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCGAGTAGCTGGGATTGCA
  GGTGCATGCCACCATGTCTGGCTGATTTTTGTATTTTTAGTACAGACAGGATTTCGCCTT
  GTTAGCCAGGCGGGTCTCCAACTCCCGACCTCAGGTGATCTGCCTGCCTCGGCCTCCCAA
  AGCGCTGGGATTACAGACGTGAGCCACGGTGCCTGGCTTCAGTAAGGACTCTTGAAGCCA
  CTGTACTGCCCTTCACTCTTTGGAAACTCTTTCCGGATCCTCCAGCGGCAGCAGCTGGGC
  AGGATGGCAAAGTCAGCCATGTCCTGGGAGCCGAAGCGCCAGGTGGCGTAGCACCTGTAG
  GCACAGTGCCGCAGCCGGCTGTTGGTGGAATCCACATCCAGCGCCAGCAAGGGCTCCTGG
  TAGAGCAGGAGGAACTGCAGGACGTGTCTGGACAGGACCAGCTTCCTGAACAGCTCTGAG
  GTGGTGATGCAGGCCCCCGGCTTTTTCCGGCAGCACAGCTCCTCCAGGCACCTGTGGCTC
  TCANGGAGTTGAGATGGGAGGCAGCTTCCACACTGGCACCAGACGGGGCTATCCCTGGAT
  CTAGGAGTCGCCTCCTTTCTAAGCAGCTGTATCTCCTCTGGTTGTCCAGGAATCGGGGGT
  GTGTCATGGAAGGCCAGGGGCAGCCTGGACAAATCTGTGAAAGTCATCACAAGTCCTTGG
  GACTTCTTGGCCCTTTGACATCTTTGCAGAACTTCCTCCCTGATAGG
  
• Restriction Sites: NotI-NotI
• ACCN: AB209709
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AB209709.1, BAD92946.1
• RefSeq Size: 2215 bp
• RefSeq ORF: 2215 bp
• Locus ID: 5027
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]'