Myosin (MYL4) (NM_002476) Human Untagged Clone

CAT#: SC118608

MYL4 (untagged)-Human myosin, light chain 4, alkali, atrial, embryonic (MYL4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL4
• Synonyms: ALC1; AMLC; GT1; PRO1957
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118608 sequence for NM_002476 edited (data generated by NextGen Sequencing)
  ATGGCTCCCAAGAAGCCTGAGCCTAAGAAGGAGGCAGCCAAGCCAGCTCCAGCTCCAGCT
  CCAGCCCCTGCACCAGCCCCTGCCCCAGCTCCTGAGGCTCCCAAGGAACCTGCCTTTGAC
  CCCAAGAGTGTAAAGATAGACTTCACTGCCGACCAGATTGAAGAGTTCAAAGAGGCCTTT
  TCATTGTTTGACCGGACCCCGACTGGAGAGATGAAGATCACCTACGGCCAGTGCGGGGAT
  GTACTGCGGGCCCTGGGCCAGAACCCTACCAATGCCGAGGTGCTGCGTGTGCTGGGCAAG
  CCCAAGCCTGAAGAGATGAATGTCAAGATGCTGGACTTTGAGACGTTCTTGCCCATCCTG
  CAGCACATTTCCCGCAACAAGGAGCAGGGCACCTATGAGGACTTCGTGGAGGGCCTGCGT
  GTCTTTGACAAGGAGAGCAATGGCACGGTCATGGGTGCTGAGCTTCGGCACGTCCTTGCC
  ACCCTGGGAGAGAAGATGACTGAGGCTGAAGTGGAGCAGCTGTTAGCTGGGCAAGAGGAT
  GCCAATGGCTGCATCAATTATGAAGCCTTTGTCAAGCACATCATGTCAGGGTGA
  
  Clone variation with respect to NM_002476.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002476 unedited
  NTTCATTACCCCGCCCGTTGNAGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATAT
  AAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCC
  GCGAATTCGGCACCAGGACGCCACGTCTCTCGGTTTCTTCTTAGATCACTCCTCTGCCAA
  AGATCCCAACAAGACAACATGGCTCCCAAGAAGCCTGAGCCTAAGAAGGAGGCAGCCAAG
  CCAGCTCCAGCTCCAGCTCCAGCCCCTGCACCAGCCCCTGCCCCAGCTCCTGAGGCTCCC
  AAGGAACCTGCCTTTGACCCCAAGAGTGTAAAGATAGACTTCACTGCCGACCAGATTGAA
  GAGTTCAAAGAGGCCTTTTCATTGTTTGACCGGACCCCGACTGGAGAGATGAAGATCACC
  TACGGCCAGTGCGGNGATGTACTGCGGGCCCTGGGCCAGAACCCTACCAATGCCGAGGTG
  CTGCGTGTGCTGGGCAAGCCAAGCCTGAGAGATGAATGTCAAGATGCTGGACTTTGAGAC
  GTTCTTGCCCATCCTGCAGCACATTTTCCGCAACAGGGAGCAGGGCACCTATGAGGACTT
  CGTGGAGGGCCTGCGTGTCTTTGACCAGGGAGAGCATGGCACGGTCATGGGGTGCTGAGC
  TTCGGCACGTCCTTTGCCACCCTGGNAGAAGAAATGACTGAGGGCTGAGTGGACNCACTG
  TTANCTGGGCCAGAGGATGCCAAGGGCTGCATCAATTATTGAAGCCTTTTCCAGCACATC
  AGGTCCGGGTGAAACAAAATCCTTCAGGTGCTGGGCCTTGGCTTTAACCCTACCAGGGTG
  ATTAAAAAAAGGCCCGGGCTGGTGAACCGAAAGGGAACCCCGAATATAACCCACCCCGTG
  CCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002476
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002476.2, NP_002467.1
• RefSeq Size: 855 bp
• RefSeq ORF: 594 bp
• Locus ID: 4635
• Cytogenetics: 17q21.32
• Gene Summary: 'Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks the 5' exon but has an alternate 5' end, as compared to variant 1.