Myosin Phosphatase (PPP1R12A) (NM_002480) Human Untagged Clone

CAT#: SC118610

PPP1R12A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R12A
• Synonyms: M130; MBS; MYPT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001143886 edited
  GGGCGGCCGCGAATTCGGCACGAGGCCCTCTCCGCCTCCCCCTTCCCCCCCTCGCGATAA
  GAAGACCCGGCGGCAGGAGAGGGGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAG
  CAGCTGAAACGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCGGTGGTGAAGCGCCAG
  AAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGCTTGCTCCAGCGGCGAC
  ACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGAC
  GGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTG
  GTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCA
  GCAGCTTCCTGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTA
  GGGGCTGTCAACAGTGAAGGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAA
  GAGCTACTTCAAAATGAAGTTAATCGGCAAGGGGTTGATATAGAAGCAGCTCGAAAGGAA
  GAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAGTGGTCATATAAATGAT
  GTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGCTATACG
  GAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGC
  TGGACACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTG
  GACAATCTGTGTGATATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCA
  GATGAAGACATTTTAGGATATTTAGAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGT
  GAAAAACGGGACAAGAAATCTCCACTAATTGAATCAACAGCAAATATGGACAATAATCAG
  TCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACCAGAGAAAAATGCATCC
  CGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAGGATGAG
  TCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGAT
  AAGACAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCT
  GTAGCTGTTACAACACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAA
  AAGTTTCCAACCACAGCTACAAAAATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCT
  CCTGCAACTTGGAGGTTAGGACTTAGAAAGACGGGCAGCTATGGTGCACTTGCTGAAATC
  ACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGTTACACGTTCAGCTTCA
  AGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAAGGAACT
  AGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAA
  GAGAAAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAA
  TGGGAAGATGATCTTAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGT
  TGCTCCTTTGGTAGAAGACAAGATGATTTGATTAGTTCTAGTGTTCCAAGCACCACATCA
  ACACCAACAGTTACCTCTGCAGCTGGGCTTCAGAAAAGCCTGCTTTCCAGCACAAGCACT
  ACTACAAAGATTACAACGGGTTCTTCCTCAGCAGGCACACAAAGCAGTACCTCAAATCGT
  TTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACCATT
  CCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACT
  GCTGGCACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCACTCCT
  GTTAGGGATGAAGAGTCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCT
  AGAAGATCAACACAGGGAGTGACATTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGA
  AGAAGTCGTTCTACCCGAACCAGAGAACAAGAAAATGAAGAAAAAGAAAAAGAGGAAAAA
  GAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAACATCTAGAGAAGAT
  GAATATAAACAAAAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGGCCAGTA
  TCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTAT
  GCTTCAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGA
  GGAATAACAAAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAA
  GATAAATCACAACCTAAATCAATCAGAGAACGACGACGACCAAGAGAGAAAAGAAGATCT
  ACAGGAGTTTCATTTTGGACACAAGATAGTGATGAAAATGAACAAGAACAACAATCAGAC
  ACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCATTTCTAGATATGAAACC
  AGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCATACAGT
  TACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTT
  AAAAAGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGAT
  ACAAATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAA
  AGATTTGCTGATAGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGA
  AGAATATCTGAAATGGAAGAAGAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAG
  AGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAAAAAAAA
  AAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCG
  GGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAG
  TGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCT
  TCTATAATAT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002480 unedited
  CTATAGGGCGGCCGCGAATTCGGCACGAGGCCCTCTCCGCCTCCCCCTTCCCCCCCTCGC
  GATAAGAAGACCCGGCGGCAGGAGAGGGGATGAAGATGGCGGACGCGAAGCAGAAGCGGA
  ACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCGGTGGTGAAGC
  GCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGCTTGCTCCAGCG
  GCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATG
  TGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGT
  TTCTGGTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTAC
  ATGCAGCAGCTTCCTGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCAC
  ATGTAGGGGCTGTCAACAGTGAAGGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAA
  TGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGGTTGATATAGAAGCAGCTCGAA
  GGAAGAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAGTGGTCATATAATG
  ATGTCCGGCATGCAAATCTNGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGCTTACN
  GGAAGTTTAAAACTTTTATACAGGCAGGCTATGATGNNTATATTAAGACTATGATGGCTG
  GACACCTCTTATGCTGCAGCTCATTGGGTAAGAAGAGCATGTCGAATTTATGGACAATCT
  GTGTGATTGGAGATGTCACAAAAGTGCGCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002480 unedited
  TATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGAAAGTTTGC
  TTATAACTCTGATCAAGGCCCCATTTTCATCCTTTAGCCTCTGGTTGTCTGCTTTTAGGT
  CTGGTAACATTTTGAGCTCTTCTTCCATTTCAGATATTCTTCTTTCTAGAGCTCTTCGTT
  CCCTTTTTTCCATTTCCAACAGTGATCTATCAGCAAATCTTTCTTGTCTCTGGGTGGCCT
  TTTCCAACTGTAATTTAAGATCTGTTAGTTCCATATTTGTATCATGTAGCTGTGCCTTCA
  GCTTTTCATTTTCAGCTAGAATTTGTTCATAAAGCTTTTTAAAGTCAGTTGAGTCATCCT
  TTTCTAGCCTGCTGCTGTAAGGTTTTCTTTCTTCTAAGTAACTGTATGATCCAGAGCGAC
  CCAGCAAGGAATCATATCGATCACCAGCTGATGTAGAACTGGTTTCATATCTAGAAATGG
  AATCCGTCTGAGTTTCTTTCTTATTGGATCCCTCTTCTGTGTCTGATTGTTGTTCTTGTT
  CATTTTCATCACTATCTTGTGTCCAAAATGAAACTCCTGTAGATCTTCTTTTCTCTCTTG
  GTCGTCGTCGTTCTCTGATTGATTTAGGTTGTGATTTATCTTCTCCTTCTTTCTCCTCTT
  CTCTTTTTTCTCCCTCTCTTTCATTTTCTTTTGGTATTCCTCTGGAGTAAGCAGAAGTTA
  TGCCTACAAGACTATTTGGCCTGTTTAGTTGACTTGAAGCATACAGTGAACTGCTCATAN
  TANAAAGTGAANAGGATGGAGTGGGTGAACTTTGAGTTGATACTGGCCTATTACGCTGGT
  NAGTCTCATCATACGTTCTGGGAGGACTTTGGTTATATTCATCCTCCTCTAGAGTGTCTG
  ACACCCTCCTTTCCTCTTGGTCCCCTTAACCTGTTTCCCTTTTCCCCCTTTTCTTTTTCT
  NCAATTCCTGGGCCCGGGGCGGGTGAACGACTCTTCTATGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002480
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002480.1, NP_002471.1
• RefSeq Size: 4613 bp
• RefSeq ORF: 3093 bp
• Locus ID: 4659
• Cytogenetics: 12q21.2-q21.31
• Domains: ANK
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: 'Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.