Myosin Phosphatase (PPP1R12A) (NM_002480) Human Untagged Clone

CAT#: SC118610

PPP1R12A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1


  "NM_002480" in other vectors (6)

Reconstitution Protocol

USD 2,100.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP1R12A
Synonyms M130; MBS; MYPT1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_001143886 edited
GGGCGGCCGCGAATTCGGCACGAGGCCCTCTCCGCCTCCCCCTTCCCCCCCTCGCGATAA
GAAGACCCGGCGGCAGGAGAGGGGATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAG
CAGCTGAAACGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCGGTGGTGAAGCGCCAG
AAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGCTTGCTCCAGCGGCGAC
ACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATGTGGAC
GGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTG
GTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCA
GCAGCTTCCTGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTA
GGGGCTGTCAACAGTGAAGGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAA
GAGCTACTTCAAAATGAAGTTAATCGGCAAGGGGTTGATATAGAAGCAGCTCGAAAGGAA
GAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAGTGGTCATATAAATGAT
GTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGCTATACG
GAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGC
TGGACACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTG
GACAATCTGTGTGATATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCA
GATGAAGACATTTTAGGATATTTAGAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGT
GAAAAACGGGACAAGAAATCTCCACTAATTGAATCAACAGCAAATATGGACAATAATCAG
TCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACCAGAGAAAAATGCATCC
CGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAGGATGAG
TCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGAT
AAGACAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCT
GTAGCTGTTACAACACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAA
AAGTTTCCAACCACAGCTACAAAAATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCT
CCTGCAACTTGGAGGTTAGGACTTAGAAAGACGGGCAGCTATGGTGCACTTGCTGAAATC
ACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGTTACACGTTCAGCTTCA
AGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAAGGAACT
AGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAA
GAGAAAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAA
TGGGAAGATGATCTTAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGT
TGCTCCTTTGGTAGAAGACAAGATGATTTGATTAGTTCTAGTGTTCCAAGCACCACATCA
ACACCAACAGTTACCTCTGCAGCTGGGCTTCAGAAAAGCCTGCTTTCCAGCACAAGCACT
ACTACAAAGATTACAACGGGTTCTTCCTCAGCAGGCACACAAAGCAGTACCTCAAATCGT
TTGTGGGCTGAGGATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACCATT
CCTGTTGCTCCAACTGTTGTAAATGCTGCAGCTTCTACCACAACCCTGACTACAACTACT
GCTGGCACTGTCTCCTCCACAACAGAGGTCAGGGAGAGACGCAGATCATACCTCACTCCT
GTTAGGGATGAAGAGTCTGAATCCCAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCT
AGAAGATCAACACAGGGAGTGACATTAACTGATCTTCAAGAAGCTGAGAAAACAATAGGA
AGAAGTCGTTCTACCCGAACCAGAGAACAAGAAAATGAAGAAAAAGAAAAAGAGGAAAAA
GAGAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAACATCTAGAGAAGAT
GAATATAAACAAAAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGGCCAGTA
TCAACTTCAAGTTCAACCACTCCATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTAT
GCTTCAAGTCAACTAAACAGGCCAAATAGTCTTGTAGGCATAACTTCTGCTTACTCCAGA
GGAATAACAAAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAAGAGGAGAAAGAAGGAGAA
GATAAATCACAACCTAAATCAATCAGAGAACGACGACGACCAAGAGAGAAAAGAAGATCT
ACAGGAGTTTCATTTTGGACACAAGATAGTGATGAAAATGAACAAGAACAACAATCAGAC
ACAGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCATTTCTAGATATGAAACC
AGTTCTACATCAGCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCATACAGT
TACTTAGAAGAAAGAAAACCTTACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTT
AAAAAGCTTTATGAACAAATTCTAGCTGAAAATGAAAAGCTGAAGGCACAGCTACATGAT
ACAAATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAGGCCACCCAGAGACAAGAA
AGATTTGCTGATAGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTCTAGAAAGA
AGAATATCTGAAATGGAAGAAGAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAG
AGGCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAAAAAAAA
AAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCG
GGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAG
TGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCT
TCTATAATAT
>OriGene 5' read for NM_002480 unedited
CTATAGGGCGGCCGCGAATTCGGCACGAGGCCCTCTCCGCCTCCCCCTTCCCCCCCTCGC
GATAAGAAGACCCGGCGGCAGGAGAGGGGATGAAGATGGCGGACGCGAAGCAGAAGCGGA
ACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACCTCGAGCCTCCGGTGGTGAAGC
GCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGCTTGCTCCAGCG
GCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAATG
TGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGT
TTCTGGTAGAAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTAC
ATGCAGCAGCTTCCTGTGGATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCAC
ATGTAGGGGCTGTCAACAGTGAAGGAGATACACCTTTAGATATTGCGGAGGAGGAGGCAA
TGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGGTTGATATAGAAGCAGCTCGAA
GGAAGAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAGTGGTCATATAATG
ATGTCCGGCATGCAAATCTNGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGCTTACN
GGAAGTTTAAAACTTTTATACAGGCAGGCTATGATGNNTATATTAAGACTATGATGGCTG
GACACCTCTTATGCTGCAGCTCATTGGGTAAGAAGAGCATGTCGAATTTATGGACAATCT
GTGTGATTGGAGATGTCACAAAAGTGCGCAAA
>OriGene 3' read for NM_002480 unedited
TATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGAAAGTTTGC
TTATAACTCTGATCAAGGCCCCATTTTCATCCTTTAGCCTCTGGTTGTCTGCTTTTAGGT
CTGGTAACATTTTGAGCTCTTCTTCCATTTCAGATATTCTTCTTTCTAGAGCTCTTCGTT
CCCTTTTTTCCATTTCCAACAGTGATCTATCAGCAAATCTTTCTTGTCTCTGGGTGGCCT
TTTCCAACTGTAATTTAAGATCTGTTAGTTCCATATTTGTATCATGTAGCTGTGCCTTCA
GCTTTTCATTTTCAGCTAGAATTTGTTCATAAAGCTTTTTAAAGTCAGTTGAGTCATCCT
TTTCTAGCCTGCTGCTGTAAGGTTTTCTTTCTTCTAAGTAACTGTATGATCCAGAGCGAC
CCAGCAAGGAATCATATCGATCACCAGCTGATGTAGAACTGGTTTCATATCTAGAAATGG
AATCCGTCTGAGTTTCTTTCTTATTGGATCCCTCTTCTGTGTCTGATTGTTGTTCTTGTT
CATTTTCATCACTATCTTGTGTCCAAAATGAAACTCCTGTAGATCTTCTTTTCTCTCTTG
GTCGTCGTCGTTCTCTGATTGATTTAGGTTGTGATTTATCTTCTCCTTCTTTCTCCTCTT
CTCTTTTTTCTCCCTCTCTTTCATTTTCTTTTGGTATTCCTCTGGAGTAAGCAGAAGTTA
TGCCTACAAGACTATTTGGCCTGTTTAGTTGACTTGAAGCATACAGTGAACTGCTCATAN
TANAAAGTGAANAGGATGGAGTGGGTGAACTTTGAGTTGATACTGGCCTATTACGCTGGT
NAGTCTCATCATACGTTCTGGGAGGACTTTGGTTATATTCATCCTCCTCTAGAGTGTCTG
ACACCCTCCTTTCCTCTTGGTCCCCTTAACCTGTTTCCCTTTTCCCCCTTTTCTTTTTCT
NCAATTCCTGGGCCCGGGGCGGGTGAACGACTCTTCTATGGTT
Restriction Sites NotI-NotI     
ACCN NM_002480
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002480.1, NP_002471.1
RefSeq Size 4613 bp
RefSeq ORF 3093 bp
Locus ID 4659
Cytogenetics 12q21.2-q21.31
Domains ANK
Protein Families Druggable Genome
Protein Pathways Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
Gene Summary 'Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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