p95 NBS1 (NBN) (NM_002485) Human Untagged Clone

CAT#: SC118613

NBN (untagged)-Human nibrin (NBN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NBN
• Synonyms: AT-V1; AT-V2; ATV; NBS; NBS1; P95
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118613 sequence for NM_002485 edited (data generated by NextGen Sequencing)
  ATGTGGAAACTGCTGCCCGCCGCGGGCCCGGCAGGAGGAGAACCATACAGACTTTTGACT
  GGCGTTGAGTACGTTGTTGGAAGGAAAAACTGTGCCATTCTGATTGAAAATGATCAGTCG
  ATCAGCCGAAATCATGCTGTGTTAACTGCTAACTTTTCTGTAACCAACCTGAGTCAAACA
  GATGAAATCCCTGTATTGACATTAAAAGATAATTCTAAGTATGGTACCTTTGTTAATGAG
  GAAAAAATGCAGAATGGCTTTTCCCGAACTTTGAAGTCGGGGGATGGTATTACTTTTGGA
  GTGTTTGGAAGTAAATTCAGAATAGAGTATGAGCCTTTGGTTGCATGCTCTTCTTGTTTA
  GATGTCTCTGGGAAAACTGCTTTAAATCAAGCTATATTGCAACTTGGAGGATTTACTGTA
  AACAATTGGACAGAAGAATGCACTCACCTTGTCATGGTATCAGTGAAAGTTACCATTAAA
  ACAATATGTGCACTCATTTGTGGACGTCCAATTGTAAAGCCAGAATATTTTACTGAATTC
  CTGAAAGCAGTTGAGTCCAAGAAGCAGCCTCCACAAATTGAAAGTTTTTACCCACCTCTT
  GATGAACCATCTATTGGAAGTAAAAATGTTGATCTGTCAGGACGGCAGGAAAGAAAACAA
  ATCTTCAAAGGGAAAACATTTATATTTTTGAATGCCAAACAGCATAAGAAATTGAGTTCC
  GCAGTTGTCTTTGGAGGTGGGGAAGCTAGGTTGATAACAGAAGAGAATGAAGAAGAACAT
  AATTTCTTTTTGGCTCCGGGAACGTGTGTTGTTGATACAGGAATAACAAACTCACAGACC
  TTAATTCCTGACTGTCAGAAGAAATGGATTCAGTCAATAATGGATATGCTCCAAAGGCAA
  GGTCTTAGACCTATTCCTGAAGCAGAAATTGGATTGGCGGTGATTTTCATGACTACAAAG
  AATTACTGTGATCCTCAGGGCCATCCCAGTACAGGATTAAAGACAACAACTCCAGGACCA
  AGCCTTTCACAAGGCGTGTCAGTTGATGAAAAACTAATGCCAAGCGCCCCAGTGAACACT
  ACAACATACGTAGCTGACACAGAATCAGAGCAAGCAGATACATGGGATTTGAGTGAAAGG
  CCAAAAGAAATCAAAGTCTCCAAAATGGAACAAAAATTCAGAATGCTTTCACAAGACGCA
  CCCACTGTAAAGGAGTCCTGCAAAACAAGCTCTAATAATAATAGTATGGTATCAAATACT
  TTGGCTAAGATGAGAATCCCAAACTATCAGCTTTCACCAACTAAATTGCCAAGTATAAAT
  AAAAGTAAAGATAGGGCTTCTCAGCAGCAGCAGACCAACTCCATCAGAAACTACTTTCAG
  CCGTCTACCAAAAAAAGGGAAAGGGATGAAGAAAATCAAGAAATGTCTTCATGCAAATCA
  GCAAGAATAGAAACGTCTTGTTCTCTTTTAGAACAAACACAACCTGCTACACCCTCATTG
  TGGAAAAATAAGGAGCAGCATCTATCTGAGAATGAGCCTGTGGACACAAACTCAGACAAT
  AACTTATTTACAGATACAGATTTAAAATCTATTGTGAAAAATTCTGCCAGTAAATCTCAT
  GCTGCAGAAAAGCTAAGATCAAATAAAAAAAGGGAAATGGATGATGTGGCCATAGAAGAT
  GAAGTATTGGAACAGTTATTCAAGGACACAAAACCAGAGTTAGAAATTGATGTGAAAGTT
  CAAAAACAGGAGGAAGATGTCAATGTTAGAAAAAGGCCAAGGATGGATATAGAAACAAAT
  GACACTTTCAGTGATGAAGCAGTACCAGAAAGTAGCAAAATATCTCAAGAAAATGAAATT
  GGGAAGAAACGTGAACTCAAGGAAGACTCACTATGGTCAGCTAAAGAAATATCTAACAAT
  GACAAACTTCAGGATGATAGTGAGATGCTTCCAAAAAAGCTGTTATTGACTGAATTTAGA
  TCACTGGTGATTAAAAACTCTACTTCCAGAAATCCATCTGGCATAAATGATGATTATGGT
  CAACTAAAAAATTTCAAGAAATTCAAAAAGGTCACATATCCTGGAGCAGGAAAACTTCCA
  CACATCATTGGAGGATCAGATCTAATAGCTCATCATGCTCGAAAGAATACAGAACTAGAA
  GAGTGGCTAAGGCAGGAAATGGAGGTACAAAATCAACATGCAAAAGAAGAGTCTCTTGCT
  GATGATCTTTTTAGATACAATCCTTATTTAAAAAGGAGAAGATAA
  
  Clone variation with respect to NM_002485.4
  1197 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002485 unedited
  GGGCGGGCCGGGNNCGCTTTTTCTCCCCCCGGGGTTGAGATTTGTATACAACTTACTATA
  GGCGGCCGCGNAATTCGCACGAGGCCGACCGCAGGCGCCGTATCCGCGCTCGTCTAGCAG
  CCCCGGTTACGCGGTTGCACGTCGGCCCCAGCCCTGAGGAGCCGGACCGATGTGGAAACT
  GCTGCCCGCCGCGGGCCCGGCAGGAGGAGAACCATACAGACTTTTGACTGGCGTTGAGTA
  CGTTGTTGGAAGGAAAAACTGTGCCATTCTGATTGAAAATGATCAGTCGATCAGCCGAAA
  TCATGCTGTGTTAACTGCTAACTTTTCTGTAACCAACCTGAGTCAAACAGATGAAATCCC
  TGTATTGACATTAAAAGATAATTCTAAGTATGGTACCTTTGTTAATGAGGAAAAAATGCA
  GAATGGCTTTTCCCGAACTTTGAAGTCGGGGGATGGTATTACTTTTGGAGTGTTTGGAAG
  TAAATTCAGAATAGAGTATGAGCCTTTGGTTGCATGCTCTTCTTGTTTAGATGTCTCTGG
  GAAAACTGCTTTAAATCAAGCTATATTGCAACTTGGAGGATTTACTGTAAACAATTGGAC
  AGAAGAATGCACTCACCTTGTCATGGTATCAGTGAAAGTTACCATTAAAACAATATGTGC
  ACTCATTTGTGGACGTCCAATTGTAAAGCCAGAATATTTTACTGAATTCCTGAAAGCAGT
  TGAGTCCAAGAAGCAGCCTCCACAAATTGAAAGTTTTACCCACCTCTTGATGAACCATCT
  ATTGGAAAGTAAAATGTTGATCTGTCAGGACGGCAGGAAAGACAACAAATCTTCAAAAGG
  AAAACATTTTATTTTTTGGATGCCAAACAGCATAAGAAATTGGGTTCCGCCATTGGTTTT
  TGAAAGTGGGGAACCTTGGTTGTTACTCAAGGAGATGAAGAAAAACCTAATTTCTTTTTG
  GCTCCCGGAACCTGTGTTGTTGAACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002485 unedited
  CCGCGGTCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTAGGGGAAAAGGATTTTTTACTAATTGGCAAGGGAATTTAATGAGGAAA
  GGATAATTTTTTCAATAAACAGGGTTACAATGACTGGATACTTGTTTGGGGGAAAAAAAA
  AACCCTGATCCATAATTCACAACATACATTTCAATTCAGGGGGGATCACAAATTTAAAAA
  TTTAAAGTTTTTTTAATAGAGCAAAATTTTTGAAATTTTGGGGTTAAATTGGAGAGAAAA
  AATTTCAAACCAAAGAAAAAAATAAATTTGACCTTGTCAAAATTTAAAACTTTTTTTCTT
  CAAAAAACATAAAAAAAACAGTCAAGCCACAAACTAGGGGTAATATCTCAATACCTATAT
  CCGACAAAAAACTTGCATTTAAAATGTATAAAAAATTTCTATGACCCAATTATTGCTTTC
  AGGGATTTACAAATTAAAACCAAAATGAAACATCCCTACCCACCGATTGGAATGGTTAAA
  AAGGAAAAATACTGACAACACCAATATTTGTAAAGACAGGAGGTCCCAAAACTCTCATTC
  ATTATATTCATAAATTGACAAATATAAAAACTGCTATAGTAGGGCAGTCTTCCTTATAAG
  GGATTGTGGGCATTACANAAAACAATATTAATCTGGGCATTATATTCCTTAACTGTAAAA
  TGGAGACCATATGTTCCACCAGCTTCACTTTGGAATTTATGAACCTGGCCTTTTAAGAAA
  CTCAAAAGACTCCCTTTTATCCACCTAATATGCCCTGGAAATTCTACCTTTAAAGTTTCC
  CTGTTCTTTTCCATGTCCTACCACTATTATTATTTAAGGGTCCTAACCTCTTTATATTAT
  TTCAATAATTCAACCTGGGCCCAATGCGCTGTGANAAAAAATTGCAGAATGGCTCCCCCC
  ATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002485
• Insert Size: 4420 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002485.4, NP_002476.2
• RefSeq Size: 4639 bp
• RefSeq ORF: 2265 bp
• Locus ID: 4683
• Cytogenetics: 8q21.3
• Domains: FHA
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• Gene Summary: 'Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).