NDUFA2 (NM_002488) Human Untagged Clone

CAT#: SC118616

NDUFA2 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa (NDUFA2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFA2
• Synonyms: B8; CD14; CIB8; MC1DN13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002488 edited
  ATGGCGGCGGCCGCAGCAAGTCGAGGAGTCGGGGCAAAGCTGGGCCTGCGTGAGATTCGC
  ATCCACTTATGTCAGCGCTCGCCCGGCAGCCAGGGCGTCAGGGACTTCATTGAGAAACGC
  TACGTGGAGCTGAAGAAGGCGAATCCCGACCTACCCATCCTAATCCGCGAATGCTCCGAT
  GTGCAGCCCAAGCTCTGGGCCCGCTACGCATTTGGCCAAGAGACGAATGTCCCTTTGAAC
  AACTTCAGTGCTGATCAGGTAACCAGAGCCCTGGAGAACGTTCTAAGTGGTAAAGCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002488 unedited
  ATTCGGCACGAGCTGGAATTGGCGACTTCGATATTAACAAGGATGGCGGCGGCCGCAGCA
  AGTCGAGGAGTCGGGGCAAAGCTGGGCCTGCGTGAGATTCGCATCCACTTATGTCAGCGC
  TCGCCCGGCAGCCAGGGCGTCAGGGACTTCATTGAGAAACGCTACGTGGAGCTGAAGAAG
  GCGAATCCCGACCTACCCATCCTAATCCGCGAATGCTCCGATGTGCAGCCCAAGCTCTGG
  GCCCGCTACGCATTTGGCCAAGAGACGAATGTCCCTTTGAACAACTTCAGTGCTGATCAG
  GTAACCAGAGCCCTGGAGAACGTTCTAAGTGGTAAAGCCTGAAGCCTCCACTGAGGATTA
  AGAGCAACAGCCCCAGAGCCTGGGCTCTGCTGGACTTAGTATAATGTGAAAAAATGTGTT
  CTCCTATTCCTCATAAAGCTTTGTGCTGTAAAAAAAAAAAAAAAAAAAAAAACTCGACTC
  TAGATTGCGGCCGCGGTCATAGCNTGTTTCCTGAACAGATCCCGGGTGGGCATCCTGTGA
  CCCCTTCCCCAGTGCCTCTCCCTGGCCTTGGAAGGTTGCCACTCCAGNGGCCCACCCACC
  CTTGGTCTAATAAAAATTAAGTTGCATCATTTTGGCTGACTAGGTGTCCNTTATATATAT
  GGGANGGGGGGGGGGGGGGGNGNGNNNNGNGGNNNNNNNGNNNNNNNNNNNNAANNNNAN
  NNNNGGGNCNNNGGGGGGGNNNNNGGGNGNNNNNNNNGGGNGNGGGGGNNNGCANNNNGG
  TGNGNGGCCGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002488 unedited
  TCTGGACCGGCGGCCGCAATCTAGNAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTACAG
  CACAAGCTTTATGAGGAATAGGAGAACACATTTTTTTCACATTATACTAAGTCCAGCAGA
  GCCCAGGCTCTGGGGCTGTTGCTCTTAATCCTCAGTGGAGGCTTCAGGCTTTACCACTTA
  AAACGTTCTCCAGGGCTCTGGTTACCTGATCAGCACTGAAGTTGTTCAAAGGGACATTCG
  TCTCTTGGCCAAATGCGTAGCGGGCCCAGAGCTTGGGCTGCACATCGGAGCATTCGCGGA
  TTAGGATGGGTAGGTCGGGATTCGCCTTCTTCAGCTCCACGTAGCGTTTCTCAATGAAGT
  CCCTGACGCCCTGTCTGCCGGGCGAGCGCTGACATAAGTGGATGCCAATCTCACGCAAGC
  CCCTCTTTGCCCCCACTCCTCCACTTGTTGCCCCCCGCTTCCTCCTTTTTTTCATTCCCC
  CCCTCTCCCCCTTTCTCCTCTCCCCCCCCCTCTCCCCCCCCCCTTTCTTTTTCCACCCCT
  CTCTCCCCCCCCACCTTCTCTTTCCTCCCCCCCCCCCCTCCCTTTCTCCCCCCCTCTCCC
  GCCCTCTCTTTCCCTTCCCGCAAATTACCCACATCACCCCTCNGCTCCTCCCAACACGCC
  NTACCTACCACATGCGCGATGCCCCCCCCCGCTCCCCTCCTCCCCTCCCCCCTTTTCCCT
  CCCCCCCTCCCCTCATCCCTCCTTCCCCTCATCATCTTTTCCGCACNACCTCTCACCCCC
  CTCTTTTACCATCCCCCCCATCCTCCCTCCTTTACTACCTCTATATTCCCTCCTCCCCAT
  CCCCCTCCCCTTTACTTCTCCCCCTCTTTTTCCCCCCCCCTTCCCTCCCTTCCCCCCCCC
  CCCTTTTCCCCCACCTCCTCTTTCCCCACCCCTTTTCCCCCCCCTCTTCCGTCCCTCCCC
  TCCTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002488
• Insert Size: 430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002488.2, NP_002479.1
• RefSeq Size: 600 bp
• RefSeq ORF: 300 bp
• Locus ID: 4695
• Cytogenetics: 5q31.3
• Domains: L51_S25_CI-B8
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1).