NEUROD1 (NM_002500) Human Untagged Clone

CAT#: SC118625

NEUROD1 (untagged)-Human neurogenic differentiation 1 (NEUROD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEUROD1
• Synonyms: BETA2; BHF-1; bHLHa3; MODY6; NEUROD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002500, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCAAATCGTACAGCGAGAGTGGGCTGATGGGCGAGCCTCAGCCCCAAGGTCCTCCAAGCTGGACAG
  ACGAGTGTCTCAGTTCTCAGGACGAGGAGCACGAGGCAGACAAGAAGGAGGACGACCTCGAAGCCATGAA
  CGCAGAGGAGGACTCACTGAGGAACGGGGGAGAGGAGGAGGACGAAGATGAGGACCTGGAAGAGGAGGAA
  GAAGAGGAAGAGGAGGATGACGATCAAAAGCCCAAGAGACGCGGCCCCAAAAAGAAGAAGATGACTAAGG
  CTCGCCTGGAGCGTTTTAAATTGAGACGCATGAAGGCTAACGCCCGGGAGCGGAACCGCATGCACGGACT
  GAACGCGGCGCTAGACAACCTGCGCAAGGTGGTGCCTTGCTATTCTAAGACGCAGAAGCTGTCCAAAATC
  GAGACTCTGCGCTTGGCCAAGAACTACATCTGGGCTCTGTCGGAGATCCTGCGCTCAGGCAAAAGCCCAG
  ACCTGGTCTCCTTCGTTCAGACGCTTTGCAAGGGCTTATCCCAACCCACCACCAACCTGGTTGCGGGCTG
  CCTGCAACTCAATCCTCGGACTTTTCTGCCTGAGCAGAACCAGGACATGCCCCCCCACCTGCCGACGGCC
  AGCGCTTCCTTCCCTGTACACCCCTACTCCTACCAGTCGCCTGGGCTGCCCAGTCCGCCTTACGGTACCA
  TGGACAGCTCCCATGTCTTCCACGTTAAGCCTCCGCCGCACGCCTACAGCGCAGCGCTGGAGCCCTTCTT
  TGAAAGCCCTCTGACTGATTGCACCAGCCCTTCCTTTGATGGACCCCTCAGCCCGCCGCTCAGCATCAAT
  GGCAACTTCTCTTTCAAACACGAACCGTCCGCCGAGTTTGAGAAAAATTATGCCTTTACCATGCACTATC
  CTGCAGCGACACTGGCAGGGGCCCAAAGCCACGGATCAATCTTCTCAGGCACCGCTGCCCCTCGCTGCGA
  GATCCCCATAGACAATATTATGTCCTTCGATAGCCATTCACATCATGAGCGAGTCATGAGTGCCCAGCTC
  AATGCCATATTTCATGATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002500 unedited
  GGTTCACATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGCG
  GCGTCCGGAGCCCCAGGTTATGAGACTATCACTGCTCAGGACCTACTAACAACAAAGGAA
  ATCGAAACATGACCAAATCGTACAGCGAGAGTGGGCTGATGGGCGAGCCTCAGCCCCAAG
  GTCCTCCAAGCTGGACAGACGAGTGTCTCAGTTCTCAGGACGAGGAGCACGAGGCAGACA
  AGAAGGAGGACGACCTCGAAACCATGAACGCAGAGGAGGACTCACTGAGGAACGGGGGAG
  AGGAGGAGGACGAAGATGAGGACCTGGAAGAGGAGGAAGAAGAGGAAGAGGAGGATGACG
  ATCAAAAGCCCAAGAGACGCGGCCCCAAAAAGAAGAAGATGACTAAGGCTCGCCTGGAGC
  GTTTTAAATTGAGACGCATGAAGGCTAACGCCCGGGAGCGGAACCGCATGCACGGACTGA
  ACGCGGCGCTAGACAACCTGCGCAAGGTGGTGCCTTGCTATTCTAAGACGCAGAAGCTGT
  CCAAAATCGAGACTCTGCGCTTGGCCAAGAACTACATCTGGGCTCTGTCGGAGATCCTGC
  GCTCAGGCAAAAGCCCAGACCTGGTCTCCTTCGTTCAGACGCTTTGCAAGGGCTTATCCC
  AACCCACCACCAACCCTGTTGCGGGCTGCCTGCAACTCAATCCTCGCACTCTTCTGCCTG
  AGCAGAACCAGGACACTGCCCCCCCACCTGCCCGACGGCCAACGCTTCCTTCCCTGTACC
  ACCCCTACTCCTACCATTCGCCTGGGCTTGCCAGTCCCGCCTACGGACCCATGGACAGCT
  CCCATGTCTTCCAGTTAAGCCCTCGCGNACGCCTACAGGGCAAGGCTGGAGCCCTCTTTG
  AAAGCCTCTGACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002500 unedited
  ACGCGGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTGCAATTGGAGAGGAAAGAAT
  GCTAAGGCAACACAATAACTTTCTAAGCACTTTTCTGCTGGTTTAAATTAGTTAAATTAT
  TTTGTATAAATTAGATATAATTCTACTTTTCTGATATGTATAAAAATTGATGAAGCTGCA
  TGGTTTTAAAATAGGAAATCCACATTATAAAAAGTCATTAAAAATTCTGTACAAAATCAC
  AACAAAATAATTCAGCATGGGAAAGGTAACAAGTAGTAAAATGCTGGTTGAAAAATATAT
  ATTTATATATATTTTAATTGGCCCATTACTAGTTTAAAAATTGCATAGATCCTAATTATT
  GCTTGTGATTTTGTTATCCCGATCAGATAATTAATACGATCTGAATACAGCCACACCAAA
  TTCGTGGTGTATTTTTTAAACTTTACTGTATTTTTTTATTTTTTAAATAGAAGAGCTATA
  GAAAATAATACATAAGGCGAACAGGAACTTTGATCCCCTGTTTCTTCCAAAGGCTGTAAT
  GACAATAAATACATATATCACTACAAGCTTGGAGTATTTGCACTTTGCAGCAGTAGTACC
  CAAAGGGCTGCCTTTTGTAAACACGACAGTCACTGTTAGCACAGTGGGTTCGTTTCCCGG
  AAATGGTGAAACTGGCGTGCCCTCTATCATGAAATATGGCATTGAGCTGGGCACTCATGA
  CTCGCTCATGATGTGAATGGCTATCGGACGACATAATATTGTCTATGGGGATCTCNCACC
  GAGGGGCAACGGTCGCCTGAAAATATTGATCCGCGGCCTTTGGCCCCCTGCCATGGTCCC
  TGCAGAATAATGCATGGTAAGGCTTATTTTTTTCCAACTCGGCGGACGTTCCGGGTTAAA
  AGAAAATTCCATTGTGCCTAACGGCGGCCTCAAGGGCCCTCAAAGGAAAGCTGGGCATTA
  TTCAAAGGCCTTTAAGAAGGCCCCACGCTTCCCTTAAGGCCCCGCGAAGCTTACCGGAAT
  ACTAGGAGCCCCC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002500
• Insert Size: 2020 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002500.1, NP_002491.1
• RefSeq Size: 3002 bp
• RefSeq ORF: 1071 bp
• Locus ID: 4760
• Cytogenetics: 2q31.3
• Domains: HLH
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.