NEUROD1 (NM_002500) Human Untagged Clone

CAT#: SC118625

NEUROD1 (untagged)-Human neurogenic differentiation 1 (NEUROD1)


  "NM_002500" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEUROD1
Synonyms BETA2; BHF-1; bHLHa3; MODY6; NEUROD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002500, the custom clone sequence may differ by one or more nucleotides


ATGACCAAATCGTACAGCGAGAGTGGGCTGATGGGCGAGCCTCAGCCCCAAGGTCCTCCAAGCTGGACAG
ACGAGTGTCTCAGTTCTCAGGACGAGGAGCACGAGGCAGACAAGAAGGAGGACGACCTCGAAGCCATGAA
CGCAGAGGAGGACTCACTGAGGAACGGGGGAGAGGAGGAGGACGAAGATGAGGACCTGGAAGAGGAGGAA
GAAGAGGAAGAGGAGGATGACGATCAAAAGCCCAAGAGACGCGGCCCCAAAAAGAAGAAGATGACTAAGG
CTCGCCTGGAGCGTTTTAAATTGAGACGCATGAAGGCTAACGCCCGGGAGCGGAACCGCATGCACGGACT
GAACGCGGCGCTAGACAACCTGCGCAAGGTGGTGCCTTGCTATTCTAAGACGCAGAAGCTGTCCAAAATC
GAGACTCTGCGCTTGGCCAAGAACTACATCTGGGCTCTGTCGGAGATCCTGCGCTCAGGCAAAAGCCCAG
ACCTGGTCTCCTTCGTTCAGACGCTTTGCAAGGGCTTATCCCAACCCACCACCAACCTGGTTGCGGGCTG
CCTGCAACTCAATCCTCGGACTTTTCTGCCTGAGCAGAACCAGGACATGCCCCCCCACCTGCCGACGGCC
AGCGCTTCCTTCCCTGTACACCCCTACTCCTACCAGTCGCCTGGGCTGCCCAGTCCGCCTTACGGTACCA
TGGACAGCTCCCATGTCTTCCACGTTAAGCCTCCGCCGCACGCCTACAGCGCAGCGCTGGAGCCCTTCTT
TGAAAGCCCTCTGACTGATTGCACCAGCCCTTCCTTTGATGGACCCCTCAGCCCGCCGCTCAGCATCAAT
GGCAACTTCTCTTTCAAACACGAACCGTCCGCCGAGTTTGAGAAAAATTATGCCTTTACCATGCACTATC
CTGCAGCGACACTGGCAGGGGCCCAAAGCCACGGATCAATCTTCTCAGGCACCGCTGCCCCTCGCTGCGA
GATCCCCATAGACAATATTATGTCCTTCGATAGCCATTCACATCATGAGCGAGTCATGAGTGCCCAGCTC
AATGCCATATTTCATGATTAG


>OriGene 5' read for NM_002500 unedited
GGTTCACATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGCG
GCGTCCGGAGCCCCAGGTTATGAGACTATCACTGCTCAGGACCTACTAACAACAAAGGAA
ATCGAAACATGACCAAATCGTACAGCGAGAGTGGGCTGATGGGCGAGCCTCAGCCCCAAG
GTCCTCCAAGCTGGACAGACGAGTGTCTCAGTTCTCAGGACGAGGAGCACGAGGCAGACA
AGAAGGAGGACGACCTCGAAACCATGAACGCAGAGGAGGACTCACTGAGGAACGGGGGAG
AGGAGGAGGACGAAGATGAGGACCTGGAAGAGGAGGAAGAAGAGGAAGAGGAGGATGACG
ATCAAAAGCCCAAGAGACGCGGCCCCAAAAAGAAGAAGATGACTAAGGCTCGCCTGGAGC
GTTTTAAATTGAGACGCATGAAGGCTAACGCCCGGGAGCGGAACCGCATGCACGGACTGA
ACGCGGCGCTAGACAACCTGCGCAAGGTGGTGCCTTGCTATTCTAAGACGCAGAAGCTGT
CCAAAATCGAGACTCTGCGCTTGGCCAAGAACTACATCTGGGCTCTGTCGGAGATCCTGC
GCTCAGGCAAAAGCCCAGACCTGGTCTCCTTCGTTCAGACGCTTTGCAAGGGCTTATCCC
AACCCACCACCAACCCTGTTGCGGGCTGCCTGCAACTCAATCCTCGCACTCTTCTGCCTG
AGCAGAACCAGGACACTGCCCCCCCACCTGCCCGACGGCCAACGCTTCCTTCCCTGTACC
ACCCCTACTCCTACCATTCGCCTGGGCTTGCCAGTCCCGCCTACGGACCCATGGACAGCT
CCCATGTCTTCCAGTTAAGCCCTCGCGNACGCCTACAGGGCAAGGCTGGAGCCCTCTTTG
AAAGCCTCTGACTG
>OriGene 3' read for NM_002500 unedited
ACGCGGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTGCAATTGGAGAGGAAAGAAT
GCTAAGGCAACACAATAACTTTCTAAGCACTTTTCTGCTGGTTTAAATTAGTTAAATTAT
TTTGTATAAATTAGATATAATTCTACTTTTCTGATATGTATAAAAATTGATGAAGCTGCA
TGGTTTTAAAATAGGAAATCCACATTATAAAAAGTCATTAAAAATTCTGTACAAAATCAC
AACAAAATAATTCAGCATGGGAAAGGTAACAAGTAGTAAAATGCTGGTTGAAAAATATAT
ATTTATATATATTTTAATTGGCCCATTACTAGTTTAAAAATTGCATAGATCCTAATTATT
GCTTGTGATTTTGTTATCCCGATCAGATAATTAATACGATCTGAATACAGCCACACCAAA
TTCGTGGTGTATTTTTTAAACTTTACTGTATTTTTTTATTTTTTAAATAGAAGAGCTATA
GAAAATAATACATAAGGCGAACAGGAACTTTGATCCCCTGTTTCTTCCAAAGGCTGTAAT
GACAATAAATACATATATCACTACAAGCTTGGAGTATTTGCACTTTGCAGCAGTAGTACC
CAAAGGGCTGCCTTTTGTAAACACGACAGTCACTGTTAGCACAGTGGGTTCGTTTCCCGG
AAATGGTGAAACTGGCGTGCCCTCTATCATGAAATATGGCATTGAGCTGGGCACTCATGA
CTCGCTCATGATGTGAATGGCTATCGGACGACATAATATTGTCTATGGGGATCTCNCACC
GAGGGGCAACGGTCGCCTGAAAATATTGATCCGCGGCCTTTGGCCCCCTGCCATGGTCCC
TGCAGAATAATGCATGGTAAGGCTTATTTTTTTCCAACTCGGCGGACGTTCCGGGTTAAA
AGAAAATTCCATTGTGCCTAACGGCGGCCTCAAGGGCCCTCAAAGGAAAGCTGGGCATTA
TTCAAAGGCCTTTAAGAAGGCCCCACGCTTCCCTTAAGGCCCCGCGAAGCTTACCGGAAT
ACTAGGAGCCCCC
Restriction Sites ECoRI-NOT     
ACCN NM_002500
Insert Size 2020 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002500.1, NP_002491.1
RefSeq Size 3002 bp
RefSeq ORF 1071 bp
Locus ID 4760
Cytogenetics 2q31.3
Domains HLH
Protein Families Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways Maturity onset diabetes of the young
Gene Summary 'This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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