Moesin (MSN) (NM_002444) Human Untagged Clone

CAT#: SC118629

MSN (untagged)-Human moesin (MSN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSN
• Synonyms: HEL70; IMD50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002444 edited
  ATGCCCAAAACGATCAGTGTGCGTGTGACCACCATGGATGCAGAGCTGGAGTTTGCCATC
  CAGCCCAACACCACCGGGAAGCAGCTATTTGACCAGGTGGTGAAAACTATTGGCTTGAGG
  GAAGTTTGGTTCTTTGGTCTGCAGTACCAGGACACTAAAGGTTTCTCCACCTGGCTGAAA
  CTCAATAAGAAGGTGACTGCCCAGGATGTGCGGAAGGAAAGCCCCCTGCTCTTTAAGTTC
  CGTGCCAAGTTCTACCCTGAGGATGTGTCCGAGGAATTGATTCAGGACATCACTCAGCGC
  CTGTTCTTTCTGCAAGTGAAAGAGGGCATTCTCAATGATGATATTTACTGCCCGCCTGAG
  ACCGCTGTGCTGCTGGCCTCGTATGCTGTCCAGTCTAAGTATGGCGACTTCAATAAGGAA
  GTGCATAAGTCTGGCTACCTGGCCGGAGACAAGTTGCTCCCGCAGAGAGTCCTGGAACAG
  CACAAACTCAACAAGGACCAGTGGGAGGAGCGGATCCAGGTGTGGCATGAGGAACACCGT
  GGCATGCTCAGGGAGGATGCTGTCCTGGAATATCTGAAGATTGCTCAAGATCTGGAGATG
  TATGGTGTGAACTACTTCAGCATCAAGAACAAGAAAGGCTCAGAGCTGTGGCTGGGGGTG
  GATGCCCTGGGTCTCAACATCTATGAGCAGAATGACAGACTAACTCCCAAGATAGGCTTC
  CCCTGGAGTGAAATCAGGAACATCTCTTTCAATGATAAGAAATTTGTCATCAAGCCCATT
  GACAAAAAAGCCCCGGACTTCGTCTTCTATGCTCCCCGGCTGCGGATTAACAAGCGGATC
  TTGGCCTTGTGCATGGGGAACCATGAACTATACATGCGCCGTCGCAAGCCTGATACCATT
  GAGGTGCAGCAGATGAAGGCACAGGCCCGGGAGGAGAAGCACCAGAAGCAGATGGAGCGT
  GCTATGCTGGAAAATGAGAAGAAGAAGCGTGAAATGGCAGAGAAGGAGAAAGAGAAGATT
  GAACGGGAGAAGGAGGAGCTGATGGAGAGGCTGAAGCAGATCGAGGAACAGACTAAGAAG
  GCTCAGCAAGAACTGGAAGAACAGACCCGTAGGGCTCTGGAACTTGAGCAGGAACGGAAG
  CGTGCCCAGAGCGAGGCTGAAAAGCTGGCCAAGGAGCGTCAAGAAGCTGAAGAGGCCAAG
  GAGGCCTTGCTGCAGGCCTCCCGGGACCAGAAAAAGACTCAGGAACAGCTGGCCTTGGAA
  ATGGCAGAGCTGACAGCTCGAATCTCCCAGCTGGAGATGGCCCGACAGAAGAAGGAGAGT
  GAGGCTGTGGAGTGGCAGCAGAAGGCCCAGATGGTACAGGAAGACTTGGAGAAGACCCGT
  GCTGAGCTGAAGACTGCCATGAGTACACCTCATGTGGCAGAGCCTGCTGAGAATGAGCAG
  GATGAGCAGGATGAGAATGGGGCAGAGGCTAGTGCTGACCTACGGGCTGATGCTATGGCC
  AAGGACCGCAGTGAGGAGGAACGTACCACTGAGGCAGAGAAGAATGAGCGTGTGCAGAAG
  CACCTGAAGGCCCTCACTTCGGAGCTGGCCAATGCCAGAGATGAGTCCAAGAAGACTGCC
  AATGACATGATCCATGCTGAGAACATGCGACTGGGCCGAGACAAATACAAGACCCTGCGC
  CAGATCCGGCAGGGCAACACCAAGCAGCGCATTGACGAATTTGAGTCTATGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002444 unedited
  NGGACGGTGCANGATTTGTATACGACTCACTATAGGCGGCCGCGAAATCGGCACGAGGGG
  CCGAATCCAAGCCGTGTGTACTGCGTGCTCAGCACTGCCCGACAGTCCTAGCTAAACTTC
  GCCAACTCCGCTGCCTTTGCCGCCACCATGCCCAAAACGATCAGTGTGCGTGTGACCACC
  ATGGATGCAGAGCTGGAGTTTGCCATCCAGCCCAACACCACCGGGAAGCAGCTATTTGAC
  CAGGTGGTGAAAACTATTGGCTTGAGGGAAGTTTGGTTCTTTGGTCTGCAGTACCAGGAC
  ACTAAAGGTTTCTCCACCTGGCTGAAACTCAATAAGAAGGTGACTGCCCAGGATGTGCGG
  AAGGAAAGCCCCCTGCTCTTTAAGTTCCGTGCCAAGTTCTACCCTGAGGATGTGTCCGAG
  GAATTGATTCAGGACATCACTCAGCGCCTGTTCTTTCTGCAAGTGAAAGAGGGCATTCTC
  AATGATGATATTTACTGCCCGCCTGAGACCGCTGTGCTGCTGGCCTCGTATGCTGTCCAG
  TCTAAGTATGGCGACTTCAATAAGGAAGTGCATAAGTCTGGCTACCTGGCCGGAGACAAG
  TTGCTCCCGCAGAGAGTCCTGGAACAGCACAAACTCAACAAGGACCAGTGGGAGGAGCGG
  ATCCAGGTGTGGCATGAGGAACACCGTGGCATGCTCAGGGAGGATGCTGTCCTGNAATAT
  CTGAAGATTGCTCAAGATCTGGAGATGTATGGTGTGAACTACTTCAGCATCAAGAACAAG
  AAGGCTCANAGCTGTGGCTGGGGGGTGGATGCCCCTGGTCTCACATCTATGAGCAGAATG
  ACAGACTAACTNCCAGATAGGCTTCCNCTGNAGTGAAATCANGAACATCTCTTTTCATGA
  TNAGAAATTTGTCATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002444 unedited
  CGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTGAAGTCTTCCTA
  AATTTTATTGGCACTGAAATAGAACATGGATTGAGCATAAACCCCTCCAAAAACAATTTT
  TAAAAAACCCAAAAAGTACACAAAAAACCCCTGAATACAAAATCTAACCTTTTCCCCCAG
  CCTCCCTAAGGGTAAGTTACTGACTTTAAGGCAGCTATTAATAGATTGCCCCACAATTCC
  AGGTTTCAATTTAGCCAATATAGGACATATCACCAAGTGAGCTAATTCACAGCAATGCAC
  ACAAGACTCCTCAAGGTCAGGCACAGAGTGGGGGGTGGTGGCCAGGGGGAATTGAGGGAG
  GCTCTAAGCTAGGGGCACTGCATGGTGGGACAGGATGGCCCCTTGAGGACTGAACCCTGG
  GGAGAAGACAAACCGTTATAATAAAAACAAATAACAAGTACTTTAAGAATGGATTGTTTG
  ACCTATAGTGACAGATGACATCACTAATACTGAAAGCTTCTTATTTAATAATTTTGGCAA
  AACGTAATTTTGAAAAAAGAAAATGCTTTCCAGGTGTGGGGGCGGAAAAGAATGAGGCCA
  AAAAATTCCTGCCCCAAAACTAATATCTTTCTATGGTGCCTTACCCAGGAAAGCTTAAAC
  CAATTCTTAAATTCCACATAGGCCCTAACCCTGCGGAAGAAGAACAGCCCCTCGACACCA
  AAAAGCGGCGGATGCTTCTGTGTTGAAAACAGGGGCGGGGTGTTGAACGGTCTTGTCCTA
  ATTCGCCTGCTCTTTGGTATAACATTCAAAGGTAATTAATCTGGTTTTATAACTTGTCAC
  AAGTTCTTTATACACGGTNGTACATCATTCTATTTAGCGTACCATTACAAGTGATCTTTC
  TTTCTCTACGGCGCCTATATCTTCCTTCCGTAACATCCAACATATCCTGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002444
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002444.2, NP_002435.1
• RefSeq Size: 3981 bp
• RefSeq ORF: 1734 bp
• Locus ID: 4478
• Cytogenetics: Xq12
• Domains: B41, ERM
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: 'Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008]'