Midkine (MDK) (NM_002391) Human Untagged Clone

CAT#: SC118659

MDK (untagged)-Human midkine (neurite growth-promoting factor 2) (MDK), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MDK
• Synonyms: ARAP; MK; NEGF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118659 sequence for NM_002391 edited (data generated by NextGen Sequencing)
  ATGCAGCACCGAGGCTTCCTCCTCCTCACCCTCCTCGCCCTACTGGCGCTCACCTCCGCG
  GTCGCCAAAAAGAAAGATAAGGTGAAGAAGGGCGGCCCGGGGAGCGAGTGCGCTGAGTGG
  GCCTGGGGGCCCTGCACCCCCAGCAGCAAGGATTGCGGCGTGGGTTTCCGCGAGGGCACC
  TGCGGGGCCCAGACCCAGCGCATCCGGTGCAGGGTGCCCTGCAACTGGAAGAAGGAGTTT
  GGAGCCGACTGCAAGTACAAGTTTGAGAACTGGGGTGCGTGTGATGGGGGCACAGGCACC
  AAAGTCCGCCAAGGCACCCTGAAGAAGGCGCGCTACAATGCTCAGTGCCAGGAGACCATC
  CGCGTCACCAAGCCCTGCACCCCCAAGACCAAAGCAAAGGCCAAAGCCAAGAAAGGGAAG
  GGAAAGGACTAG
  
  Clone variation with respect to NM_002391.3
  42 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002391 unedited
  NATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAGCGAAGC
  AGCGCGGGCAGCGAGCGAGATGCAGCACCGAGGCTTCCTCCTCCTCACCCTCCTCGCCCT
  ACTGGCGCTCACCTCCGCGGTCGCCAAAAAGAAAGATAAGGTGAAGAAGGGCGGCCCGGG
  GAGCGAGTGCGCTGAGTGGGCCTGGGGGCCCTGCACCCCCAGCAGCAAGGATTGCGGCGT
  GGGTTTCCGCGAGGGCACCTGCGGGGCCCAGACCCAGCGCATCCGGTGCAGGGTGCCCTG
  CAACTGGAAGAAGGAGTTTGGAGCCGACTGCAAGTACAAGTTTGAGAACTGGGGTGCGTG
  TGATGGGGGCACAGGCACCAAAGTCCGCCAAGGCACCCTGAAGAAGGCGCGCTACAATGC
  TCAGTGCCAGGAGACCATCCGCGTCACCAAGCCCTGCACCCCCAAGACCAAAGCAAAGGC
  CAAAGCCAAGAAAGGGAAGGGAAAGGACTAGACGCCAAGCCTGGATGCCAAGGAGCCCCT
  GGTGTCACATGGGGCCTGGCCCACGCCCTCCCTCTCCCAGGCCCGAGATGTGACCCACCA
  GTGCCTTCTGTCTGCTCGTTAGCTTTAATCAATCATGCCCTGCCTTGTCCCTCTCACTCC
  CCAGCCCCACCCCTAAGTGCCCAAAGTGGGGAGGGACAAGGGATTCTGGGAAGCTTGAGC
  CTCCCCCAAGCATGTGAGTCCCAGAGCCGCTTTTGNTCTTCCCCACATTNCCTTACTAAG
  AACACTCAAATAAACTGACTTTTTTCCCCCAAAAAAAAANAAAACTCGCTCTAGATTGCG
  NCGCGTCATAGCTGTTTCTGAACAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002391 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGGGGAAAAATCAGTTTA
  TTTGATGTGTTTCTTAGTAATGGAATTGTGGGGAAGAACAAAAGCGGGCTCTGGGACTCA
  CATTGCTTTGGGGGAGGCTCAAGCTTCCCAGAATCCCTTGTCCCTCCCCACTTTGGGCAC
  TTAAGGGTGGGGCTGGGGAGTGAGAGGGACAAGGCAGGGCATGATTGATTAAAGCTAACG
  AGCAGACAGAAGGCACTGGTGGGTCACATCTCGGGCCTGGGAGAGGGAGGGCGTGGGCCA
  AGCCCCATGTGACACCAGGGGCTCCTTGGCATCCAGGCTTGGCGTCTAGTCCTTTCCCTT
  CCCTTTCTTGGCTTTGGCCTTTGCTTTGGTCTTGGGGGTGCAAGGCTTGGTGACGCGGAT
  GGTCTCCTGGCACTGAGCATTGTAGCGCGCCTTCTTCAAGGTGCCTTGGCGGACTTTGGT
  GCCTGTGCCCCCATCACACGCACCCCAGTTCTCAAACTTGTACTTGCAGTCGGCTCCAAA
  CTCCTTCTTCCAGTTGCAGGGCACCCTGCACCGGATGCGCTGGGTCTGGGCCCCGCAAGT
  GCCCTCGCGGAAACCCACGCCGCAATCCTTGCTGCTGGGGGTGCAAGGCCCCCAGGCCCA
  CTCAGNGCACTCGCTCCCCGGGCCGCCCTTCTTCACCTTATCTTTCTTTTTGGCGACCGC
  GGATGTGATCGCCACTATGGCCAAGAAGGTGATGAAGAGGAAACCTTGGTGCTGCATCTC
  GCTCGCTGCCCGCGCCTGCTTGCTCCCCCCGTGCCCGAATTCCGGGCGCCCTTAGTGAGT
  CGTATTACAAATTCTGAGGGTTACTAACGAGCTCTGCTTATTAGACCTCCACGTAACGCC
  TACCCCATTTGGTAACGGGGGCGGGTATTACAACATTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002391
• Insert Size: 750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002391.3, NP_002382.1
• RefSeq Size: 866 bp
• RefSeq ORF: 432 bp
• Locus ID: 4192
• Cytogenetics: 11p11.2
• Domains: PTN
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, and 5 encode the same isoform (a).