ME1 (NM_002395) Human Untagged Clone

CAT#: SC118663

ME1 (untagged)-Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ME1
• Synonyms: HUMNDME; MES
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118663 sequence for NM_002395 edited (data generated by NextGen Sequencing)
  ATGGAGCCCGAAGCCCCCCGTCGCCGCCACACCCATCAGCGCGGCTACCTGCTGACACGG
  AACCCTCACCTCAACAAGGACTTGGCCTTTACCCTGGAAGAGAGACAGCAATTGAACATT
  CATGGATTGTTGCCACCTTCCTTCAACAGTCAGGAGATCCAGGTTCTTAGAGTAGTAAAA
  AATTTCGAGCATCTGAACTCTGACTTTGACAGGTATCTTCTCTTAATGGATCTCCAAGAT
  AGAAATGAAAAACTCTTTTATAGAGTGCTGACATCTGACATTGAGAAATTCATGCCTATT
  GTTTATACTCCCACTGTGGGTCTGGCTTGCCAACAATATAGTTTGGTGTTTCGGAAGCCA
  AGAGGTCTCTTTATTACTATCCACGATCGAGGGCATATTGCTTCAGTTCTCAATGCATGG
  CCAGAAGATGTCATCAAGGCCATTGTGGTGACTGATGGAGAGCGTATTCTTGGCTTGGGA
  GACCTTGGCTGTAATGGAATGGGCATCCCTGTGGGTAAATTGGCTCTATATACAGCTTGC
  GGAGGGATGAATCCTCAAGAATGTCTGCCTGTCATTCTGGATGTGGGAACCGAAAATGAG
  GAGTTACTTAAAGATCCACTCTACATTGGACTACGGCAGAGAAGAGTAAGAGGTTCTGAA
  TATGATGATTTTTTGGACGAATTCATGGAGGCAGTTTCTTCCAAGTATGGCATGAATTGC
  CTTATTCAGTTTGAAGATTTTGCCAATGTGAATGCATTTCGTCTCCTGAACAAGTATCGA
  AACCAGTATTGCACATTCAATGATGATATTCAAGGAACAGCATCTGTTGCAGTTGCAGGT
  CTCCTTGCAGCTCTTCGAATAACCAAGAACAAACTGTCTGATCAAACAATACTATTCCAA
  GGAGCTGGAGAGGCTGCCCTAGGGATTGCACACCTGATTGTGATGGCCTTGGAAAAAGAA
  GGTTTACCAAAAGAGAAAGCCATCAAAAAGATATGGCTGGTTGATTCAAAAGGATTAATA
  GTTAAGGGACGTGCTTCCTTAACACAAGAGAAAGAGAAGTTTGCCCATGAACATGAAGAA
  ATGAAGAACCTAGAAGCCATTGTTCAAGAAATAAAACCAACTGCCCTCATAGGAGTTGCT
  GCAATTGGTGGTGCATTCTCAGAACAAATTCTCAAAGATATGGCTGCCTTCAATGAACGG
  CCTATTATTTTTGCTTTGAGTAATCCAACTAGCAAAGCAGAATGTTCTGCAGAGCAGTGC
  TACAAAATAACCAAGGGACGTGCAATTTTTGCCAGTGGCAGTCCTTTTGATCCAGTCACT
  CTTCCAAATGGACAGACCCTATATCCTGGCCAAGGCAACAATTCCTATGTGTTCCCTGGA
  GTTGCTCTTGGTGTTGTGGCGTGTGGATTGAGGCAGATCACAGATAATATTTTCCTCACT
  ACTGCTGAGGTTATAGCTCAGCAAGTGTCAGATAAACACTTGGAAGAGGGTCGGCTTTAT
  CCTCCTTTGAATACCATTAGAGATGTTTCTCTGAAAATTGCAGAAAAGATTGTGAAAGAT
  GCATACCAAGAAAAGACAGCCACAGTTTATCCTGAACCGCAAAACAAAGAAGCATTTGTC
  CGCTCCCAGATGTATAGTACTGATTATGACCAGATTCTACCTGATTGTTATTCTTGGCCT
  GAAGAGGTGCAGAAAATACAGACCAAAGTTGACCAGTAG
  
  Clone variation with respect to NM_002395.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002395 unedited
  AACATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCGCCGCCAC
  AGCTGCAGTCAGCACCGTCACCCCAGCAGCATCCGCCGCCTGCACCGCGCGTGCGGCCCG
  CCCCGGCCTGACCCCGCCGCCGAACCCGGCGCCAGCCATGGAGCCCGAAGCCCCCCGTCG
  CCGCCACACCCATCAGCGCGGCTACCTGCTGACACGGAACCCTCACCTCAACAAGGACTT
  GGCCTTTACCCTGGAAGAGAGACAGCAATTGAACATTCATGGATTGTTGCCACCTTCCTT
  CAACAGTCAGGAGATCCAGGTTCTTAGAGTAGTAAAAAATTTCGAGCATCTGAACTCTGA
  CTTTGACAGGTATCTTCTCTTAATGGATCTCCAAGATAGAAATGAAAAACTCTTTTATAG
  AGTGCTGACATCTGACATTGAGAAATTCATGCCTATTGTTTATACTCCCACTGTGGGTCT
  GGCTTGCCAACAATATAGTTTGGTGTTTCGGAAGCCAAGAGGTCTCTTTATTACTATCCA
  CGATCGAGGGCATATTGCTTCAGTTCTCAATGCATGGCCAGAAGATGTCATCAAGGCCAT
  TGTGGTGACTGATGGAGAGCGTATTCTTGGCTTGGGAGACCTTGGCTGTAATGGAATGGG
  CATCCCTGTGGGTAAATTGGCTCTATATACAGCTTGCGGAGGGATGAATCCTCAAGATGT
  CTGCCTGTCATTCTGGATGTGGGAACCGAAATGANGAGTTACTTNAAGACCACTCTACAT
  TGGACTACNGCAGAGAAGAAGTAGAAGTTCTGATATGATGATTNTTTGGACGAATTCATG
  GAGGCAGTTTCTTCCAGTATGGCATGAATTGCCTTATTCAGNTTGAAGAATTNTGCCAAT
  GTGAATGGCATTTCGTCTCCCTGAACAGTATCGAAAACAGTATTGCACATTNCATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002395 unedited
  CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGTTTTAAT
  GTTGGTTATGTCAATTTATTAGTAATTTTGGTCTTCCCATTAGTGTCCTGTATAAAAATG
  ATGAGTTTCTCCGTAGTACGTACAACAGCTTTTAAAGAGAACGTAGGCAGAATAATTCAG
  ACAGAAACCATAAATAACCAGAAGGCAAAGTGAAGCAAAATTGTCTCATGTGATGTTTAT
  CCCAATTTATATCGATATTCTTCTATCAATTTTTAGACTGTTAAAGTAAAATCTTAATCT
  AAGTGTCTTATGAATCATTATAAAAGATTCCAACCTTTAAAAATTATGAAAGGTTTAAAG
  ACCTCATTAATAGAGTTAGAAATGTTTGCTATTATCCTACTGGTCAACTTTGGTCTGTAT
  TTTCTGCACCTCTTCAGGCCAAGAATAACAATCAGGTAGAATCTGGTCATAATCAGTACT
  ATACATCTGGGAGCGGACAAATGCTTCTTTGTTTTGCGGTTCAGGATAAACTGTGGCTGT
  CTTTTCTTGGTATGCATCTTTCACAATCTTTTCTGCAATTTTCAGAGAAACATCTCTAAT
  GGTATTCAAAGGAGGATAAAGCCGACCCTCTTCCAAGTGTTTATCTGACACTTGCTGAGC
  TATAACCTCAGCAGTAGTGAGGAAAATATTATCTGTGATCTGCCTCAATCCACACGCCAC
  AACACCAAGAGCAACTCCAGGGAACACATANGAATTGGTGCCTTGGCCAGNATATAGGGT
  CTGTCCATTTGGAAGATGACTGGATCAAAGGACTGCCACTGGCAAAATTGCACGTCCCTT
  GGGTATTTGTAGCACTGCTCTGCAAACATTCTGTTTGCTAGNTGGATACTCAAACAAAAT
  AATAGCCGTCATTGAAGGCACCATACTTTTGGAATTGTCTGGGAAGCCCACCATGCGCAC
  TCTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002395
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002395.2, NP_002386.1
• RefSeq Size: 2212 bp
• RefSeq ORF: 1719 bp
• Locus ID: 4199
• Cytogenetics: 6q14.2
• Domains: malic
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism
• Gene Summary: 'This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]'