ME1 (NM_002395) Human Untagged Clone

CAT#: SC118663

ME1 (untagged)-Human malic enzyme 1, NADP(+)-dependent, cytosolic (ME1)


  "NM_002395" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ME1
Synonyms HUMNDME; MES
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118663 sequence for NM_002395 edited (data generated by NextGen Sequencing)
ATGGAGCCCGAAGCCCCCCGTCGCCGCCACACCCATCAGCGCGGCTACCTGCTGACACGG
AACCCTCACCTCAACAAGGACTTGGCCTTTACCCTGGAAGAGAGACAGCAATTGAACATT
CATGGATTGTTGCCACCTTCCTTCAACAGTCAGGAGATCCAGGTTCTTAGAGTAGTAAAA
AATTTCGAGCATCTGAACTCTGACTTTGACAGGTATCTTCTCTTAATGGATCTCCAAGAT
AGAAATGAAAAACTCTTTTATAGAGTGCTGACATCTGACATTGAGAAATTCATGCCTATT
GTTTATACTCCCACTGTGGGTCTGGCTTGCCAACAATATAGTTTGGTGTTTCGGAAGCCA
AGAGGTCTCTTTATTACTATCCACGATCGAGGGCATATTGCTTCAGTTCTCAATGCATGG
CCAGAAGATGTCATCAAGGCCATTGTGGTGACTGATGGAGAGCGTATTCTTGGCTTGGGA
GACCTTGGCTGTAATGGAATGGGCATCCCTGTGGGTAAATTGGCTCTATATACAGCTTGC
GGAGGGATGAATCCTCAAGAATGTCTGCCTGTCATTCTGGATGTGGGAACCGAAAATGAG
GAGTTACTTAAAGATCCACTCTACATTGGACTACGGCAGAGAAGAGTAAGAGGTTCTGAA
TATGATGATTTTTTGGACGAATTCATGGAGGCAGTTTCTTCCAAGTATGGCATGAATTGC
CTTATTCAGTTTGAAGATTTTGCCAATGTGAATGCATTTCGTCTCCTGAACAAGTATCGA
AACCAGTATTGCACATTCAATGATGATATTCAAGGAACAGCATCTGTTGCAGTTGCAGGT
CTCCTTGCAGCTCTTCGAATAACCAAGAACAAACTGTCTGATCAAACAATACTATTCCAA
GGAGCTGGAGAGGCTGCCCTAGGGATTGCACACCTGATTGTGATGGCCTTGGAAAAAGAA
GGTTTACCAAAAGAGAAAGCCATCAAAAAGATATGGCTGGTTGATTCAAAAGGATTAATA
GTTAAGGGACGTGCTTCCTTAACACAAGAGAAAGAGAAGTTTGCCCATGAACATGAAGAA
ATGAAGAACCTAGAAGCCATTGTTCAAGAAATAAAACCAACTGCCCTCATAGGAGTTGCT
GCAATTGGTGGTGCATTCTCAGAACAAATTCTCAAAGATATGGCTGCCTTCAATGAACGG
CCTATTATTTTTGCTTTGAGTAATCCAACTAGCAAAGCAGAATGTTCTGCAGAGCAGTGC
TACAAAATAACCAAGGGACGTGCAATTTTTGCCAGTGGCAGTCCTTTTGATCCAGTCACT
CTTCCAAATGGACAGACCCTATATCCTGGCCAAGGCAACAATTCCTATGTGTTCCCTGGA
GTTGCTCTTGGTGTTGTGGCGTGTGGATTGAGGCAGATCACAGATAATATTTTCCTCACT
ACTGCTGAGGTTATAGCTCAGCAAGTGTCAGATAAACACTTGGAAGAGGGTCGGCTTTAT
CCTCCTTTGAATACCATTAGAGATGTTTCTCTGAAAATTGCAGAAAAGATTGTGAAAGAT
GCATACCAAGAAAAGACAGCCACAGTTTATCCTGAACCGCAAAACAAAGAAGCATTTGTC
CGCTCCCAGATGTATAGTACTGATTATGACCAGATTCTACCTGATTGTTATTCTTGGCCT
GAAGAGGTGCAGAAAATACAGACCAAAGTTGACCAGTAG

Clone variation with respect to NM_002395.4
>OriGene 5' read for NM_002395 unedited
AACATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCGCCGCCAC
AGCTGCAGTCAGCACCGTCACCCCAGCAGCATCCGCCGCCTGCACCGCGCGTGCGGCCCG
CCCCGGCCTGACCCCGCCGCCGAACCCGGCGCCAGCCATGGAGCCCGAAGCCCCCCGTCG
CCGCCACACCCATCAGCGCGGCTACCTGCTGACACGGAACCCTCACCTCAACAAGGACTT
GGCCTTTACCCTGGAAGAGAGACAGCAATTGAACATTCATGGATTGTTGCCACCTTCCTT
CAACAGTCAGGAGATCCAGGTTCTTAGAGTAGTAAAAAATTTCGAGCATCTGAACTCTGA
CTTTGACAGGTATCTTCTCTTAATGGATCTCCAAGATAGAAATGAAAAACTCTTTTATAG
AGTGCTGACATCTGACATTGAGAAATTCATGCCTATTGTTTATACTCCCACTGTGGGTCT
GGCTTGCCAACAATATAGTTTGGTGTTTCGGAAGCCAAGAGGTCTCTTTATTACTATCCA
CGATCGAGGGCATATTGCTTCAGTTCTCAATGCATGGCCAGAAGATGTCATCAAGGCCAT
TGTGGTGACTGATGGAGAGCGTATTCTTGGCTTGGGAGACCTTGGCTGTAATGGAATGGG
CATCCCTGTGGGTAAATTGGCTCTATATACAGCTTGCGGAGGGATGAATCCTCAAGATGT
CTGCCTGTCATTCTGGATGTGGGAACCGAAATGANGAGTTACTTNAAGACCACTCTACAT
TGGACTACNGCAGAGAAGAAGTAGAAGTTCTGATATGATGATTNTTTGGACGAATTCATG
GAGGCAGTTTCTTCCAGTATGGCATGAATTGCCTTATTCAGNTTGAAGAATTNTGCCAAT
GTGAATGGCATTTCGTCTCCCTGAACAGTATCGAAAACAGTATTGCACATTNCATG
>OriGene 3' read for NM_002395 unedited
CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGTTTTAAT
GTTGGTTATGTCAATTTATTAGTAATTTTGGTCTTCCCATTAGTGTCCTGTATAAAAATG
ATGAGTTTCTCCGTAGTACGTACAACAGCTTTTAAAGAGAACGTAGGCAGAATAATTCAG
ACAGAAACCATAAATAACCAGAAGGCAAAGTGAAGCAAAATTGTCTCATGTGATGTTTAT
CCCAATTTATATCGATATTCTTCTATCAATTTTTAGACTGTTAAAGTAAAATCTTAATCT
AAGTGTCTTATGAATCATTATAAAAGATTCCAACCTTTAAAAATTATGAAAGGTTTAAAG
ACCTCATTAATAGAGTTAGAAATGTTTGCTATTATCCTACTGGTCAACTTTGGTCTGTAT
TTTCTGCACCTCTTCAGGCCAAGAATAACAATCAGGTAGAATCTGGTCATAATCAGTACT
ATACATCTGGGAGCGGACAAATGCTTCTTTGTTTTGCGGTTCAGGATAAACTGTGGCTGT
CTTTTCTTGGTATGCATCTTTCACAATCTTTTCTGCAATTTTCAGAGAAACATCTCTAAT
GGTATTCAAAGGAGGATAAAGCCGACCCTCTTCCAAGTGTTTATCTGACACTTGCTGAGC
TATAACCTCAGCAGTAGTGAGGAAAATATTATCTGTGATCTGCCTCAATCCACACGCCAC
AACACCAAGAGCAACTCCAGGGAACACATANGAATTGGTGCCTTGGCCAGNATATAGGGT
CTGTCCATTTGGAAGATGACTGGATCAAAGGACTGCCACTGGCAAAATTGCACGTCCCTT
GGGTATTTGTAGCACTGCTCTGCAAACATTCTGTTTGCTAGNTGGATACTCAAACAAAAT
AATAGCCGTCATTGAAGGCACCATACTTTTGGAATTGTCTGGGAAGCCCACCATGCGCAC
TCTTGG
Restriction Sites NotI-NotI     
ACCN NM_002395
Insert Size 2100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002395.2, NP_002386.1
RefSeq Size 2212 bp
RefSeq ORF 1719 bp
Locus ID 4199
Cytogenetics 6q14.2
Domains malic
Protein Pathways Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism
Gene Summary 'This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.