ME2 (NM_002396) Human Untagged Clone

CAT#: SC118666

ME2 (untagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ME2
• Synonyms: ODS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118666 sequence for NM_002396 edited (data generated by NextGen Sequencing)
  ATGTTGTCCCGGTTAAGAGTAGTTTCCACCACTTGTACTTTGGCATGTCGACATTTGCAC
  ATAAAAGAAAAAGGCAAGCCACTTATGCTGAACCCAAGAACAAACAAGGGAATGGCATTT
  ACTTTACAAGAACGACAAATGCTTGGTCTTCAAGGACTTCTACCTCCCAAAATAGAGACA
  CAAGATATTCAAGCCTTACGATTTCATAGAAACTTGAAGAAAATGACTAGCCCTTTGGAA
  AAATATATCTACATAATGGGAATACAAGAAAGAAATGAGAAATTGTTTTATAGAATACTG
  CAAGATGACATTGAGAGTTTAATGCCAATTGTATATACACCGACGGTTGGTCTTGCCTGC
  TCCCAGTATGGACACATCTTTAGAAGACCTAAGGGATTATTTATTTCGATCTCAGACAGA
  GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTAGTG
  ACTGATGGAGAGAGAATTCTGGGTCTTGGAGATCTGGGTGTCTATGGAATGGGAATTCCA
  GTAGGAAAACTTTGTTTGTATACAGCTTGTGCAGGAATACGGCCTGATAGATGCCTGCCA
  GTGTGTATTGATGTGGGAACTGATAATATCGCACTCTTAAAAGACCCATTTTACATGGGC
  TTGTACCAGAAACGAGATCGCACACAACAGTATGATGACCTGATTGATGAGTTTATGAAA
  GCTATTACTGACAGATATGGCCGGAACACACTCATTCAGTTCGAAGACTTTGGAAATCAT
  AATGCATTCAGGTTCTTGAGAAAGTACCGAGAAAAATATTGTACTTTCAATGATGATATT
  CAAGGGACAGCTGCAGTAGCTCTAGCAGGTCTTCTTGCAGCACAAAAAGTTATTAGTAAA
  CCAATCTCCGAACACAAAATCTTATTCCTTGGAGCAGGAGAGGCTGCTCTTGGAATTGCA
  AATCTTATAGTTATGTCTATGGTAGAAAATGGCCTGTCAGAACAAGAGGCACAAAAGAAA
  ATCTGGATGTTTGACAAGTATGGTTTATTAGTTAAGGGACGGAAAGCAAAAATAGATAGT
  TATCAGGAACCATTTACTCACTCAGCCCCAGAGAGCATACCTGATACTTTTGAAGATGCA
  GTGAATATACTGAAGCCTTCAACTATAATTGGAGTTGCAGGTGCTGGCCGTCTTTTCACT
  CCTGATGTAATCAGAGCCATGGCCTCTATCAATGAAAGGCCTGTAATATTTGCATTAAGT
  AATCCTACAGCACAGGCAGAGTGCACGGCTGAAGAAGCATATACACTTACAGAGGGCAGG
  TGTTTGTTTGCCAGTGGCAGTCCATTTGGGCCAGTGAAACTTACAGATGGGCGAGTCTTT
  ACACCAGGTCAAGGAAACAATGTTTATATTTTTCCAGGTGTGGCTTTAGCTGTTATTCTC
  TGTAACACCCGGCATATTAGTGACAGTGTTTTCCTAGAAGCTGCAAAGGCCCTGACAAGC
  CAATTGACAGATGAAGAGCTAGCCCAAGGGAGACTTTACCCACCGCTTGCTAATATTCAG
  GAAGTTTCTATTAACATTGCTATTAAAGTTACAGAATACCTATATGCTAATAAAATGGCT
  TTCCGATACCCAGAACCTGAAGACAAGGCCAAATATGTTAAAGAAAGAACATGGCGGAGT
  GAATATGATTCCCTGCTGCCAGATGTGTATGAATGGCCAGAATCTGCATCAAGCCCTCCT
  GTGATAACAGAATAG
  
  Clone variation with respect to NM_002396.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002396 unedited
  NGGGTCAATTTTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGCTCGC
  AGAAGGGAGGGCCGGGCGGCGCGGGAGCTGAGCATCGCCAGGGCGGGCGGCAGGGCGCGG
  CCTCTCCGCCGGGTGTACCACCTGTCGCGGCGCGAGACCTCTGGTGAAAGAAAAGATGTT
  GTCCCGGTTAAGAGTAGTTTCCACCACTTGTACTTTGGCATGTCGACATTTGCACATAAA
  AGAAAAAGGCAAGCCACTTATGCTGAACCCAAGAACAAACAAGGGAATGGCATTTACTTT
  ACAAGAACGACAAATGCTTGGTCTTCAAGGACTTCTACCTCCCAAAATAGAGACACAAGA
  TATTCAAGCCTTACGATTTCATAGAAACTTGAAGAAAATGACTAGCCCTTTGGAAAAATA
  TATCTACATAATGGGAATACAAGAAAGAAATGAGAAATTGTTTTATAGAATACTGCAAGA
  TGACATTGAGAGTTTAATGCCAATTGTATATACACCGACGGTTGGTCTTGCCTGCTCCCA
  GTATGGACACATCTTTAGAAGACCTAAGGGATTATTTATTTCGATCTCAGACAGAGGTCA
  TGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTAGTGACTGA
  TGGAGAGAGAATTCTGGGTCTTGGAGATCTGGGTGTCTATGGAATGGGAATTCCAGTAGG
  AAAACTTTGTTTGTATACAGCTTGTGCAGGAATACGGCCTGATAGATGCCTGCCAGTGTG
  TATTGATGTGGGAACTGATATATCGCACTCTTAANAGACCCATTTCATGGGCTTGTACCA
  GAACGAGATCGCACACACAGTATGATGACCTGATTGATGAGTTTATGAAAGCTATTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002396 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACTGTTGAAAACAAT
  GACTTTTATTTACTTAAAGCCAGCAGTAGTTCCCATTACTCTCATAATGTTATAGTTAAG
  GCTTGATTTAGTTCCAGAAAATAAATAGGGTAAATTTTTAATATTTCCCTAGCTCTGTCT
  GCTATAGGGAATTTCAGAGTATGAAGGTAAGATGAAGCAGATATATAAGAACATTTTTAG
  ATAATGACAATTTTTCCTTAAAATTTGGTGAAAATTTAGTTTCTTCTCAAAATTCTGTAC
  TTCTATCCATAAAAGTAAATTTCTATTTTAGTAGCTCTGTAAGAACTAGGCCAGAGAAGA
  GTATTACCCATAATAGTAAATAGCAAATACTTTGGCAAGTCTGAATTAGAGTACAAGTGA
  AGACATTCACAAACACACTTTTTACATCTCCTGGATGTGGTACGGGCTGTATGTTAGAAT
  TAAAGCATCACAACTATCTGATTGTAGGGTGCTGGTGGGCAATGCAATCAATCAACACGT
  CTACCCCAACAGATGTGGAGACGCATGGAAAAAATACATCAACCAAAGTGGTCAGGGAGA
  ACAAAACACAGAAAACACCATAAAACTGAAGACATTATCTCTTCTTGTCTGAAAAAAGGG
  GTTCCCTGGAGCACAGAAAGTATTTATCAGGGGAGTGCTTCTATTCTGTTATCACAGGAN
  GGGCTTGATGCAGATTCTGGCCATTCATACACATCTGGCAGCAGGGAATCATATTCACTC
  CGCCATGTTCTTTCTTTAACATATTGGCCTTGNCTTCAGTTCTGGGTATCGGAAGCCATT
  TATTAGCATATAGTATCTGTACTTTATAGCATGTTATAGAACTTCTGATATANCAGCGTG
  GGTAAGCTCCTTGCCTCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002396
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002396.3, NP_002387.1
• RefSeq Size: 2730 bp
• RefSeq ORF: 1755 bp
• Locus ID: 4200
• Cytogenetics: 18q21.2
• Domains: malic
• Protein Pathways: Pyruvate metabolism
• Gene Summary: 'This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.