ME2 (NM_002396) Human Untagged Clone

CAT#: SC118666

ME2 (untagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_002396" in other vectors (6)

Reconstitution Protocol

USD 1,000.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ME2
Synonyms ODS1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118666 sequence for NM_002396 edited (data generated by NextGen Sequencing)
ATGTTGTCCCGGTTAAGAGTAGTTTCCACCACTTGTACTTTGGCATGTCGACATTTGCAC
ATAAAAGAAAAAGGCAAGCCACTTATGCTGAACCCAAGAACAAACAAGGGAATGGCATTT
ACTTTACAAGAACGACAAATGCTTGGTCTTCAAGGACTTCTACCTCCCAAAATAGAGACA
CAAGATATTCAAGCCTTACGATTTCATAGAAACTTGAAGAAAATGACTAGCCCTTTGGAA
AAATATATCTACATAATGGGAATACAAGAAAGAAATGAGAAATTGTTTTATAGAATACTG
CAAGATGACATTGAGAGTTTAATGCCAATTGTATATACACCGACGGTTGGTCTTGCCTGC
TCCCAGTATGGACACATCTTTAGAAGACCTAAGGGATTATTTATTTCGATCTCAGACAGA
GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTAGTG
ACTGATGGAGAGAGAATTCTGGGTCTTGGAGATCTGGGTGTCTATGGAATGGGAATTCCA
GTAGGAAAACTTTGTTTGTATACAGCTTGTGCAGGAATACGGCCTGATAGATGCCTGCCA
GTGTGTATTGATGTGGGAACTGATAATATCGCACTCTTAAAAGACCCATTTTACATGGGC
TTGTACCAGAAACGAGATCGCACACAACAGTATGATGACCTGATTGATGAGTTTATGAAA
GCTATTACTGACAGATATGGCCGGAACACACTCATTCAGTTCGAAGACTTTGGAAATCAT
AATGCATTCAGGTTCTTGAGAAAGTACCGAGAAAAATATTGTACTTTCAATGATGATATT
CAAGGGACAGCTGCAGTAGCTCTAGCAGGTCTTCTTGCAGCACAAAAAGTTATTAGTAAA
CCAATCTCCGAACACAAAATCTTATTCCTTGGAGCAGGAGAGGCTGCTCTTGGAATTGCA
AATCTTATAGTTATGTCTATGGTAGAAAATGGCCTGTCAGAACAAGAGGCACAAAAGAAA
ATCTGGATGTTTGACAAGTATGGTTTATTAGTTAAGGGACGGAAAGCAAAAATAGATAGT
TATCAGGAACCATTTACTCACTCAGCCCCAGAGAGCATACCTGATACTTTTGAAGATGCA
GTGAATATACTGAAGCCTTCAACTATAATTGGAGTTGCAGGTGCTGGCCGTCTTTTCACT
CCTGATGTAATCAGAGCCATGGCCTCTATCAATGAAAGGCCTGTAATATTTGCATTAAGT
AATCCTACAGCACAGGCAGAGTGCACGGCTGAAGAAGCATATACACTTACAGAGGGCAGG
TGTTTGTTTGCCAGTGGCAGTCCATTTGGGCCAGTGAAACTTACAGATGGGCGAGTCTTT
ACACCAGGTCAAGGAAACAATGTTTATATTTTTCCAGGTGTGGCTTTAGCTGTTATTCTC
TGTAACACCCGGCATATTAGTGACAGTGTTTTCCTAGAAGCTGCAAAGGCCCTGACAAGC
CAATTGACAGATGAAGAGCTAGCCCAAGGGAGACTTTACCCACCGCTTGCTAATATTCAG
GAAGTTTCTATTAACATTGCTATTAAAGTTACAGAATACCTATATGCTAATAAAATGGCT
TTCCGATACCCAGAACCTGAAGACAAGGCCAAATATGTTAAAGAAAGAACATGGCGGAGT
GAATATGATTCCCTGCTGCCAGATGTGTATGAATGGCCAGAATCTGCATCAAGCCCTCCT
GTGATAACAGAATAG

Clone variation with respect to NM_002396.4
>OriGene 5' read for NM_002396 unedited
NGGGTCAATTTTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGCTCGC
AGAAGGGAGGGCCGGGCGGCGCGGGAGCTGAGCATCGCCAGGGCGGGCGGCAGGGCGCGG
CCTCTCCGCCGGGTGTACCACCTGTCGCGGCGCGAGACCTCTGGTGAAAGAAAAGATGTT
GTCCCGGTTAAGAGTAGTTTCCACCACTTGTACTTTGGCATGTCGACATTTGCACATAAA
AGAAAAAGGCAAGCCACTTATGCTGAACCCAAGAACAAACAAGGGAATGGCATTTACTTT
ACAAGAACGACAAATGCTTGGTCTTCAAGGACTTCTACCTCCCAAAATAGAGACACAAGA
TATTCAAGCCTTACGATTTCATAGAAACTTGAAGAAAATGACTAGCCCTTTGGAAAAATA
TATCTACATAATGGGAATACAAGAAAGAAATGAGAAATTGTTTTATAGAATACTGCAAGA
TGACATTGAGAGTTTAATGCCAATTGTATATACACCGACGGTTGGTCTTGCCTGCTCCCA
GTATGGACACATCTTTAGAAGACCTAAGGGATTATTTATTTCGATCTCAGACAGAGGTCA
TGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTAGTGACTGA
TGGAGAGAGAATTCTGGGTCTTGGAGATCTGGGTGTCTATGGAATGGGAATTCCAGTAGG
AAAACTTTGTTTGTATACAGCTTGTGCAGGAATACGGCCTGATAGATGCCTGCCAGTGTG
TATTGATGTGGGAACTGATATATCGCACTCTTAANAGACCCATTTCATGGGCTTGTACCA
GAACGAGATCGCACACACAGTATGATGACCTGATTGATGAGTTTATGAAAGCTATTCTG
>OriGene 3' read for NM_002396 unedited
TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACTGTTGAAAACAAT
GACTTTTATTTACTTAAAGCCAGCAGTAGTTCCCATTACTCTCATAATGTTATAGTTAAG
GCTTGATTTAGTTCCAGAAAATAAATAGGGTAAATTTTTAATATTTCCCTAGCTCTGTCT
GCTATAGGGAATTTCAGAGTATGAAGGTAAGATGAAGCAGATATATAAGAACATTTTTAG
ATAATGACAATTTTTCCTTAAAATTTGGTGAAAATTTAGTTTCTTCTCAAAATTCTGTAC
TTCTATCCATAAAAGTAAATTTCTATTTTAGTAGCTCTGTAAGAACTAGGCCAGAGAAGA
GTATTACCCATAATAGTAAATAGCAAATACTTTGGCAAGTCTGAATTAGAGTACAAGTGA
AGACATTCACAAACACACTTTTTACATCTCCTGGATGTGGTACGGGCTGTATGTTAGAAT
TAAAGCATCACAACTATCTGATTGTAGGGTGCTGGTGGGCAATGCAATCAATCAACACGT
CTACCCCAACAGATGTGGAGACGCATGGAAAAAATACATCAACCAAAGTGGTCAGGGAGA
ACAAAACACAGAAAACACCATAAAACTGAAGACATTATCTCTTCTTGTCTGAAAAAAGGG
GTTCCCTGGAGCACAGAAAGTATTTATCAGGGGAGTGCTTCTATTCTGTTATCACAGGAN
GGGCTTGATGCAGATTCTGGCCATTCATACACATCTGGCAGCAGGGAATCATATTCACTC
CGCCATGTTCTTTCTTTAACATATTGGCCTTGNCTTCAGTTCTGGGTATCGGAAGCCATT
TATTAGCATATAGTATCTGTACTTTATAGCATGTTATAGAACTTCTGATATANCAGCGTG
GGTAAGCTCCTTGCCTCCTC
Restriction Sites NotI-NotI     
ACCN NM_002396
Insert Size 2880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002396.3, NP_002387.1
RefSeq Size 2730 bp
RefSeq ORF 1755 bp
Locus ID 4200
Cytogenetics 18q21.2
Domains malic
Protein Pathways Pyruvate metabolism
Gene Summary 'This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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